Journal: Frontiers in plant science
Article Title: Microscopic distribution of alkaloids in freeze-fixed stems of Phellodendron amurense .
doi: 10.3389/fpls.2023.1203768
Figure Lengend Snippet: FIGURE 2 Cryo-TOF-SIMS spectra and chemical structures of (A) berberine, (B) columbamine, (C) jatrorrhizine, (D) magnoflorine, (E) phellodendrine, (F) 8- oxoepiberberine, (G) palmatine and (H) tetrahydropalmatine. Cryo-TOF-SIMS spectra obtained from the frozen, hydrated transverse surface of a P. amurense stem from (I) fall and (J) summer in the region containing phloem, cambial zone, and xylem. Alkaloid standard chemicals were dissolved at ca. 50 mM in ACN and frozen for measurements.
Article Snippet: Alkaloid standards including berberine chloride, palmatine chloride, and magnoflorine (SIGMA-Aldrich, MA, USA), tetrahydropalmatine and jatrorrhizine chloride (Tokyo Chemical Industry Co., Ltd., Tokyo, Japan), phellodendrine (Nacalai Tesque Inc., Kyoto, Japan), 8-oxoepiberberine and columbamine frontiersin.org (MedChem Express, NJ, USA) were purchased and used as received.
Techniques: