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columbamine  (MedChemExpress)
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MedChemExpress columbamine
HPLC conditions for quantifying minor alkaloids.
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HPLC conditions for quantifying minor alkaloids.

Journal: Frontiers in Plant Science

Article Title: Microscopic distribution of alkaloids in freeze-fixed stems of Phellodendron amurense

doi: 10.3389/fpls.2023.1203768

Figure Lengend Snippet: HPLC conditions for quantifying minor alkaloids.

Article Snippet: Alkaloid standards including berberine chloride, palmatine chloride, and magnoflorine (SIGMA-Aldrich, MA, USA), tetrahydropalmatine and jatrorrhizine chloride (Tokyo Chemical Industry Co., Ltd., Tokyo, Japan), phellodendrine (Nacalai Tesque Inc., Kyoto, Japan), 8-oxoepiberberine and columbamine (MedChem Express, NJ, USA) were purchased and used as received.

Techniques:

Contents of minor alkaloids in fall and summer P. amurense stems quantified by HPLC.

Journal: Frontiers in Plant Science

Article Title: Microscopic distribution of alkaloids in freeze-fixed stems of Phellodendron amurense

doi: 10.3389/fpls.2023.1203768

Figure Lengend Snippet: Contents of minor alkaloids in fall and summer P. amurense stems quantified by HPLC.

Article Snippet: Alkaloid standards including berberine chloride, palmatine chloride, and magnoflorine (SIGMA-Aldrich, MA, USA), tetrahydropalmatine and jatrorrhizine chloride (Tokyo Chemical Industry Co., Ltd., Tokyo, Japan), phellodendrine (Nacalai Tesque Inc., Kyoto, Japan), 8-oxoepiberberine and columbamine (MedChem Express, NJ, USA) were purchased and used as received.

Techniques:

Cryo-TOF-SIMS spectra and chemical structures of (A) berberine, (B) columbamine, (C) jatrorrhizine, (D) magnoflorine, (E) phellodendrine, (F) 8-oxoepiberberine, (G) palmatine and (H) tetrahydropalmatine. Cryo-TOF-SIMS spectra obtained from the frozen, hydrated transverse surface of a P. amurense stem from (I) fall and (J) summer in the region containing phloem, cambial zone, and xylem. Alkaloid standard chemicals were dissolved at ca. 50 mM in ACN and frozen for measurements.

Journal: Frontiers in Plant Science

Article Title: Microscopic distribution of alkaloids in freeze-fixed stems of Phellodendron amurense

doi: 10.3389/fpls.2023.1203768

Figure Lengend Snippet: Cryo-TOF-SIMS spectra and chemical structures of (A) berberine, (B) columbamine, (C) jatrorrhizine, (D) magnoflorine, (E) phellodendrine, (F) 8-oxoepiberberine, (G) palmatine and (H) tetrahydropalmatine. Cryo-TOF-SIMS spectra obtained from the frozen, hydrated transverse surface of a P. amurense stem from (I) fall and (J) summer in the region containing phloem, cambial zone, and xylem. Alkaloid standard chemicals were dissolved at ca. 50 mM in ACN and frozen for measurements.

Article Snippet: Alkaloid standards including berberine chloride, palmatine chloride, and magnoflorine (SIGMA-Aldrich, MA, USA), tetrahydropalmatine and jatrorrhizine chloride (Tokyo Chemical Industry Co., Ltd., Tokyo, Japan), phellodendrine (Nacalai Tesque Inc., Kyoto, Japan), 8-oxoepiberberine and columbamine (MedChem Express, NJ, USA) were purchased and used as received.

Techniques:

Cryo-TOF-SIMS/SEM images of a freeze-fixed stem of P. amurense in fall at the transverse surface. (A) Cryo-SEM image of the freeze-etched sample after cryo-TOF-SIMS analysis. Cryo-TOF-SIMS images for positive ions of (B) total ions, (C) potassium at m / z 39, (D) phosphatidylcholine at m / z 58, (E) berberine at m / z 336, (F) columbamine and jatrorrhizine at m / z 338, (G) magnoflorine and phellodendrine at m / z 342, (H) 8-oxoepiberberine and palmatine at m / z 352, (I) tetrahydropalmatine at m / z 356. (J) An optical microscopy image of a freeze-fixed stem of P. amurense from the fall season in the sample holder showing the measured area. Scale bars are 200 μm for (A-I) and 1.0 mm for (J) .

Journal: Frontiers in Plant Science

Article Title: Microscopic distribution of alkaloids in freeze-fixed stems of Phellodendron amurense

doi: 10.3389/fpls.2023.1203768

Figure Lengend Snippet: Cryo-TOF-SIMS/SEM images of a freeze-fixed stem of P. amurense in fall at the transverse surface. (A) Cryo-SEM image of the freeze-etched sample after cryo-TOF-SIMS analysis. Cryo-TOF-SIMS images for positive ions of (B) total ions, (C) potassium at m / z 39, (D) phosphatidylcholine at m / z 58, (E) berberine at m / z 336, (F) columbamine and jatrorrhizine at m / z 338, (G) magnoflorine and phellodendrine at m / z 342, (H) 8-oxoepiberberine and palmatine at m / z 352, (I) tetrahydropalmatine at m / z 356. (J) An optical microscopy image of a freeze-fixed stem of P. amurense from the fall season in the sample holder showing the measured area. Scale bars are 200 μm for (A-I) and 1.0 mm for (J) .

Article Snippet: Alkaloid standards including berberine chloride, palmatine chloride, and magnoflorine (SIGMA-Aldrich, MA, USA), tetrahydropalmatine and jatrorrhizine chloride (Tokyo Chemical Industry Co., Ltd., Tokyo, Japan), phellodendrine (Nacalai Tesque Inc., Kyoto, Japan), 8-oxoepiberberine and columbamine (MedChem Express, NJ, USA) were purchased and used as received.

Techniques: Microscopy

Cryo-TOF-SIMS/SEM images of a freeze-fixed stem of P. amurense in summer at the transverse surface. (A) Cryo-SEM image of the freeze-etched sample after cryo-TOF-SIMS analysis. Cryo-TOF-SIMS images for positive ions of (B) total ions, (C) potassium at m / z 39, (D) phosphatidylcholine at m / z 58, (E) berberine at m / z 336, (F) columbamine and jatrorrhizine at m / z 338, (G) magnoflorine and phellodendrine at m / z 342, (H) 8-oxoepiberberine and palmatine at m / z 352, (I) tetrahydropalmatine at m / z 356. (J) Optical microscopy image of a freeze-fixed stem of P. amurense from the summer season in the sample holder showing the measured area. Scale bars are 200 μm for (A−I) and 1.0 mm for (J) .

Journal: Frontiers in Plant Science

Article Title: Microscopic distribution of alkaloids in freeze-fixed stems of Phellodendron amurense

doi: 10.3389/fpls.2023.1203768

Figure Lengend Snippet: Cryo-TOF-SIMS/SEM images of a freeze-fixed stem of P. amurense in summer at the transverse surface. (A) Cryo-SEM image of the freeze-etched sample after cryo-TOF-SIMS analysis. Cryo-TOF-SIMS images for positive ions of (B) total ions, (C) potassium at m / z 39, (D) phosphatidylcholine at m / z 58, (E) berberine at m / z 336, (F) columbamine and jatrorrhizine at m / z 338, (G) magnoflorine and phellodendrine at m / z 342, (H) 8-oxoepiberberine and palmatine at m / z 352, (I) tetrahydropalmatine at m / z 356. (J) Optical microscopy image of a freeze-fixed stem of P. amurense from the summer season in the sample holder showing the measured area. Scale bars are 200 μm for (A−I) and 1.0 mm for (J) .

Article Snippet: Alkaloid standards including berberine chloride, palmatine chloride, and magnoflorine (SIGMA-Aldrich, MA, USA), tetrahydropalmatine and jatrorrhizine chloride (Tokyo Chemical Industry Co., Ltd., Tokyo, Japan), phellodendrine (Nacalai Tesque Inc., Kyoto, Japan), 8-oxoepiberberine and columbamine (MedChem Express, NJ, USA) were purchased and used as received.

Techniques: Microscopy