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hm03  (MedChemExpress)


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    MedChemExpress hm03
    Hm03, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hm03/product/MedChemExpress
    Average 92 stars, based on 6 article reviews
    hm03 - by Bioz Stars, 2026-02
    92/100 stars

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    BAF60c interacts with GRP78 (A) Co-IP of HEK293T cells overexpressing BAF60c-HA and GRP78-FLAG. (B) HEK293T cells were co-transfected with BAF60c-GFP and GRP78-RFP plasmids for 24 h and followed by laser scanning confocal microscopy to observe the colocalization of BAF60c and GRP78. Scale bars, 20 μm.

    Journal: iScience

    Article Title: Exploring the role of SWI/SNF complex subunit BAF60c in lipid metabolism and inflammation in fish

    doi: 10.1016/j.isci.2023.108207

    Figure Lengend Snippet: BAF60c interacts with GRP78 (A) Co-IP of HEK293T cells overexpressing BAF60c-HA and GRP78-FLAG. (B) HEK293T cells were co-transfected with BAF60c-GFP and GRP78-RFP plasmids for 24 h and followed by laser scanning confocal microscopy to observe the colocalization of BAF60c and GRP78. Scale bars, 20 μm.

    Article Snippet: GRP78 agonist (Bip inducer X; MCE) and GRP78 inhibitor (HM03; MCE) were incubated with a concentration of 10 μM for different time points.

    Techniques: Co-Immunoprecipitation Assay, Transfection, Confocal Microscopy

    BAF60c regulates GRP78 expression in fish (A) Relative mRNA expression of BAF60c and GRP78 after BAF60c-siRNA treatment for 36 h in macrophage. (B) Relative mRNA expression of BAF60c and GRP78 after BAF60c-siRNA treatment for 36 h in hepatocyte. (C) Relative mRNA expression of BAF60c and GRP78 after overexpression of BAF60c for 24 h in hepatocyte. (D) Relative mRNA expression of BAF60c and GRP78 after BiP treatment for 4 h with different concentrations in macrophages. (E) Protein level of BAF60c after 10 μM BiP treatment for 4 and 8 h in macrophages. (F) Relative mRNA expression of BAF60c and GRP78 after HM03 treatment for 4 h with different concentrations in macrophages. (G) Protein level of BAF60c after 10 μM HM03 treatment for 3 and 5 h in macrophages. Data are represented as mean ± SEM (n = 3) and were analyzed using independent t test. The “∗” means a significant difference (p < 0.05), and “∗∗” means a highly significant difference (p < 0.01).

    Journal: iScience

    Article Title: Exploring the role of SWI/SNF complex subunit BAF60c in lipid metabolism and inflammation in fish

    doi: 10.1016/j.isci.2023.108207

    Figure Lengend Snippet: BAF60c regulates GRP78 expression in fish (A) Relative mRNA expression of BAF60c and GRP78 after BAF60c-siRNA treatment for 36 h in macrophage. (B) Relative mRNA expression of BAF60c and GRP78 after BAF60c-siRNA treatment for 36 h in hepatocyte. (C) Relative mRNA expression of BAF60c and GRP78 after overexpression of BAF60c for 24 h in hepatocyte. (D) Relative mRNA expression of BAF60c and GRP78 after BiP treatment for 4 h with different concentrations in macrophages. (E) Protein level of BAF60c after 10 μM BiP treatment for 4 and 8 h in macrophages. (F) Relative mRNA expression of BAF60c and GRP78 after HM03 treatment for 4 h with different concentrations in macrophages. (G) Protein level of BAF60c after 10 μM HM03 treatment for 3 and 5 h in macrophages. Data are represented as mean ± SEM (n = 3) and were analyzed using independent t test. The “∗” means a significant difference (p < 0.05), and “∗∗” means a highly significant difference (p < 0.01).

    Article Snippet: GRP78 agonist (Bip inducer X; MCE) and GRP78 inhibitor (HM03; MCE) were incubated with a concentration of 10 μM for different time points.

    Techniques: Expressing, Over Expression

    Knockdown of GRP78 in macrophages and hepatocytes can reduce ER stress, inflammation, and fatty acid biosynthesis (A) Relative mRNA expression of grp78 after GRP78-siRNA treatment for 36 h in macrophages. (B) Relative mRNA expression of ER stress-related genes after GRP78-siRNA treatment for 36 h in macrophages. (C) Relative mRNA expression of inflammatory genes after GRP78-siRNA treatment for 36 h in macrophages. (D) Relative mRNA expression of grp78 after GRP78-siRNA treatment for 36 h in hepatocytes. (E) Relative mRNA expression of ER stress-related genes after GRP78-siRNA treatment for 36 h in hepatocytes. (F) Relative mRNA expression of inflammatory genes after GRP78-siRNA treatment for 36 h in hepatocytes. (G) Relative mRNA expression of lipid metabolism-related genes after GRP78-siRNA treatment for 36 h in hepatocytes. Data are represented as mean ± SEM (n = 3) and were analyzed using independent t test. The “∗” means a significant difference (p < 0.05), and “∗∗” means a highly significant difference (p < 0.01).

    Journal: iScience

    Article Title: Exploring the role of SWI/SNF complex subunit BAF60c in lipid metabolism and inflammation in fish

    doi: 10.1016/j.isci.2023.108207

    Figure Lengend Snippet: Knockdown of GRP78 in macrophages and hepatocytes can reduce ER stress, inflammation, and fatty acid biosynthesis (A) Relative mRNA expression of grp78 after GRP78-siRNA treatment for 36 h in macrophages. (B) Relative mRNA expression of ER stress-related genes after GRP78-siRNA treatment for 36 h in macrophages. (C) Relative mRNA expression of inflammatory genes after GRP78-siRNA treatment for 36 h in macrophages. (D) Relative mRNA expression of grp78 after GRP78-siRNA treatment for 36 h in hepatocytes. (E) Relative mRNA expression of ER stress-related genes after GRP78-siRNA treatment for 36 h in hepatocytes. (F) Relative mRNA expression of inflammatory genes after GRP78-siRNA treatment for 36 h in hepatocytes. (G) Relative mRNA expression of lipid metabolism-related genes after GRP78-siRNA treatment for 36 h in hepatocytes. Data are represented as mean ± SEM (n = 3) and were analyzed using independent t test. The “∗” means a significant difference (p < 0.05), and “∗∗” means a highly significant difference (p < 0.01).

    Article Snippet: GRP78 agonist (Bip inducer X; MCE) and GRP78 inhibitor (HM03; MCE) were incubated with a concentration of 10 μM for different time points.

    Techniques: Knockdown, Expressing

    Journal: iScience

    Article Title: Exploring the role of SWI/SNF complex subunit BAF60c in lipid metabolism and inflammation in fish

    doi: 10.1016/j.isci.2023.108207

    Figure Lengend Snippet:

    Article Snippet: GRP78 agonist (Bip inducer X; MCE) and GRP78 inhibitor (HM03; MCE) were incubated with a concentration of 10 μM for different time points.

    Techniques: Virus, Recombinant, Transfection, Sequencing, Plasmid Preparation, Software