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5× reverse transcriptase (rt) buffer  (Toyobo)


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    Structured Review

    Toyobo 5× reverse transcriptase (rt) buffer
    5× Reverse Transcriptase (Rt) Buffer, supplied by Toyobo, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/5%C3%97+reverse+transcriptase+(rt)+buffer/5%C3%97+reverse+transcriptase++rt++buffer/10__2147_slash_copd__s107396-58-36-40
    Average 90 stars, based on 1 article reviews
    5× reverse transcriptase (rt) buffer - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: A Suspension Induction for Myocardial Differentiation of Rat Mesenchymal Stem Cells on Various Extracellular Matrix Proteins
    Article Snippet: The microenvironment of bone marrow-derived mesenchymal stem cells (MSCs) strictly regulates their differentiation.. In this study, we have developed a new suspension induction method for myocardial differentiation of bone marrow-derived rat MSCs (rMSCs) in vitro on various extracellular matrix (ECM) proteins.. Myocardial differentiation of rMSCs was induced with a conventional monolayer method and our suspension method.

    Article Title: The inhibitory mechanism of Cordyceps sinensis on cigarette smoke extract-induced senescence in human bronchial epithelial cells
    Article Snippet: Briefly, 1 μg of total RNA per sample was denaturated at 70°C for 10 minutes and laid on ice for 10 minutes, PCR reactions were performed in a volume of 20 μL containing 4 μL 5× reverse transcriptase (RT) buffer (Toyobo, Osaka, Japan), 1 μL RT Enzyme Mix (Toyobo), 1.0 μL (5 pmol) of each primer (sense and antisense) in the presence of PCR buffer (Toyobo).

    Article Title: The inhibitory mechanism of <em>Cordyceps sinensis</em> on cigarette smoke extract-induced senescence in human bronchial epithelial cells
    Article Snippet: Briefly, 1 μg of total RNA per sample was denaturated at 70°C for 10 minutes and laid on ice for 10 minutes, PCR reactions were performed in a volume of 20 μL containing 4 μL 5× reverse transcriptase (RT) buffer (Toyobo, Osaka, Japan), 1 μL RT Enzyme Mix (Toyobo), 1.0 μL (5 pmol) of each primer (sense and antisense) in the presence of PCR buffer (Toyobo).

    Reverse Transcription:

    Article Title: Effect of cigarette smoking on mRNA and protein levels of oxytocin receptor and on contractile sensitivity of uterine myometrium to oxytocin in pregnant women.
    Article Snippet: Objectives: Although smoking is the most important modifiable risk factor associated with preterm delivery, the underlying mechanism by which smoking stimulates premature uterine contractions is still poorly understood.. In the present study, we investigated whether cigarette smoking affects the contractile sensitivity of uterine myometrium to oxytocin in pregnant women.. Study design: Cigarette smoking habits of pregnant women were evaluated by direct interviews and by measuring exhaled carbon monoxide (CO).

    Article Title: Activation of the circAGFG1/miR-195-5p/PD-L1 axis induces lung injury in sepsis
    Article Snippet: Next, 1 μg of the RNA was mixed with Oligo-dT primers (ThermoFisher) and ddH 2 O and incubated for 5 min at 70°C. .. The sample was placed on ice, then mixed with 4 μL 5 × reverse transcriptase (RT) Buffer (ReverTra Ace qPCR RT Master Mix, TOYOBO, Osaka, Japan) and 2 μL 10 mM dNTPs (ThermoFisher), followed by a 5-min incubation at 37°C. .. Finally, 0.5 μL of RT (TOYOBO, Osaka, Japan) was added, and the sample was incubated at 42°C for 60 min and 70°C for 5 min.

    Article Title: Activation of the circAGFG1/miR-195-5p/PD-L1 axis induces lung injury in sepsis.
    Article Snippet: Next, 1 μg of the RNA was mixed with Oligo-dT primers (ThermoFisher) and ddH2O and incubated for 5 min at 70°C. .. The sample was placed on ice, then mixed with 4 μL 5 × reverse transcriptase (RT) Buffer (ReverTra Ace qPCR RT Master Mix, TOYOBO, Osaka, Japan) and 2 μL 10 mM dNTPs (ThermoFisher), followed by a 5-min incubation at 37°C. .. Finally, 0.5 μL of RT (TOYOBO, Osaka, Japan) was added, and the sample was incubated at 42°C for 60 min and 70°C for 5 min. qRT‐PCR The cDNA was diluted 10 × and 4 μL was added to each well of a 384-well plate and centrifuged at 1,000 rpm. qRT-PCR was performed using the SYBR Green PCR master mix (ThermoFisher, 4,368,708), according to the manufacturer’s instructions, the primers (Sangon Biotech, Shanghai, China) for the indicated genes, and water were mixed at a ratio of 1:1:3, added to each well and mixed again, and then qRT-PCR was performed.

    Real-time Polymerase Chain Reaction:

    Article Title: Activation of the circAGFG1/miR-195-5p/PD-L1 axis induces lung injury in sepsis
    Article Snippet: Next, 1 μg of the RNA was mixed with Oligo-dT primers (ThermoFisher) and ddH 2 O and incubated for 5 min at 70°C. .. The sample was placed on ice, then mixed with 4 μL 5 × reverse transcriptase (RT) Buffer (ReverTra Ace qPCR RT Master Mix, TOYOBO, Osaka, Japan) and 2 μL 10 mM dNTPs (ThermoFisher), followed by a 5-min incubation at 37°C. .. Finally, 0.5 μL of RT (TOYOBO, Osaka, Japan) was added, and the sample was incubated at 42°C for 60 min and 70°C for 5 min.

    Article Title: Activation of the circAGFG1/miR-195-5p/PD-L1 axis induces lung injury in sepsis.
    Article Snippet: Next, 1 μg of the RNA was mixed with Oligo-dT primers (ThermoFisher) and ddH2O and incubated for 5 min at 70°C. .. The sample was placed on ice, then mixed with 4 μL 5 × reverse transcriptase (RT) Buffer (ReverTra Ace qPCR RT Master Mix, TOYOBO, Osaka, Japan) and 2 μL 10 mM dNTPs (ThermoFisher), followed by a 5-min incubation at 37°C. .. Finally, 0.5 μL of RT (TOYOBO, Osaka, Japan) was added, and the sample was incubated at 42°C for 60 min and 70°C for 5 min. qRT‐PCR The cDNA was diluted 10 × and 4 μL was added to each well of a 384-well plate and centrifuged at 1,000 rpm. qRT-PCR was performed using the SYBR Green PCR master mix (ThermoFisher, 4,368,708), according to the manufacturer’s instructions, the primers (Sangon Biotech, Shanghai, China) for the indicated genes, and water were mixed at a ratio of 1:1:3, added to each well and mixed again, and then qRT-PCR was performed.

    Incubation:

    Article Title: Activation of the circAGFG1/miR-195-5p/PD-L1 axis induces lung injury in sepsis
    Article Snippet: Next, 1 μg of the RNA was mixed with Oligo-dT primers (ThermoFisher) and ddH 2 O and incubated for 5 min at 70°C. .. The sample was placed on ice, then mixed with 4 μL 5 × reverse transcriptase (RT) Buffer (ReverTra Ace qPCR RT Master Mix, TOYOBO, Osaka, Japan) and 2 μL 10 mM dNTPs (ThermoFisher), followed by a 5-min incubation at 37°C. .. Finally, 0.5 μL of RT (TOYOBO, Osaka, Japan) was added, and the sample was incubated at 42°C for 60 min and 70°C for 5 min.

    Article Title: Activation of the circAGFG1/miR-195-5p/PD-L1 axis induces lung injury in sepsis.
    Article Snippet: Next, 1 μg of the RNA was mixed with Oligo-dT primers (ThermoFisher) and ddH2O and incubated for 5 min at 70°C. .. The sample was placed on ice, then mixed with 4 μL 5 × reverse transcriptase (RT) Buffer (ReverTra Ace qPCR RT Master Mix, TOYOBO, Osaka, Japan) and 2 μL 10 mM dNTPs (ThermoFisher), followed by a 5-min incubation at 37°C. .. Finally, 0.5 μL of RT (TOYOBO, Osaka, Japan) was added, and the sample was incubated at 42°C for 60 min and 70°C for 5 min. qRT‐PCR The cDNA was diluted 10 × and 4 μL was added to each well of a 384-well plate and centrifuged at 1,000 rpm. qRT-PCR was performed using the SYBR Green PCR master mix (ThermoFisher, 4,368,708), according to the manufacturer’s instructions, the primers (Sangon Biotech, Shanghai, China) for the indicated genes, and water were mixed at a ratio of 1:1:3, added to each well and mixed again, and then qRT-PCR was performed.



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    Image Search Results


    Journal: iScience

    Article Title: Time course transcriptomic profiling suggests Crp/Fnr transcriptional regulation of nosZ gene in a N 2 O-reducing thermophile

    doi: 10.1016/j.isci.2024.111074

    Figure Lengend Snippet:

    Article Snippet: Maxima H Minus Reverse Transcriptase (200U/μL) with 5×RT Buffer , ThermoFisher , Cat# EP0751.

    Techniques: Virus, Recombinant, Reverse Transcription, Isolation, Control, Sequencing, Mass Spectrometry, Software, In Silico, Targeted Proteomics