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5 reverse transcriptase (rt) buffer  (Thermo Fisher)


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    Thermo Fisher 5 reverse transcriptase (rt) buffer
    5 Reverse Transcriptase (Rt) Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/5%C3%97+reverse+transcriptase+(rt)+buffer/us10844393-503-36-39
    Average 90 stars, based on 1 article reviews
    5 reverse transcriptase (rt) buffer - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Induction of Viral Mimicry Upon Loss of DHX9 and ADAR1 in Breast Cancer Cells
    Article Snippet: Ribosomal RNA was removed by an RNase-H method using RiboErase kit (Kapa Biosystems). .. After rRNA depletion, the remaining RNA was then fragmented in reverse transcriptase buffer (Life Technologies) by heating to 94 degrees for 8 minutes. .. The RNA was reverse transcribed to yield cDNA using SuperScript III RT and random hexamers (Life Technologies) per manufacturer's instructions.

    Article Title: Ethylhexadecyldimethylammonium bromide, a quaternary ammonium compound, controls inflammatory response through NRF2 pathway in a human immortalized keratinocyte cell line
    Article Snippet: Total RNA isolation was performed with PureLink RNA Mini (Invitrogen). .. 500 ng total RNA were reverse-transcribed using the thermal cycler T100 (Bio-Rad) in a total-20 μL reaction mixture: 2.5 μM of Oligo (dT) (Promega, Charbonnières-les-Bains, France), 0.5 mM of deoxy-nucleotide triphosphate (dNTP; MP Biomedicals, Illkirch, France), 1 U/μL of Superscript IV Reverse Transcriptase (Invitrogen), 1 U/μL of Recombinant RNasin Ribonuclease Inhibitor (Promega), 5 mM of dithiothreitol (Invitrogen), 5 mM of reverse transcriptase buffer (Invitrogen). .. 500 ng total RNA were reverse-transcribed using the thermal cycler T100 (Bio-Rad) in a total-20 μL reaction mixture: 2.5 μM of Oligo (dT) (Promega, Charbonnières-les-Bains, France), 0.5 mM of deoxy-nucleotide triphosphate (dNTP; MP Biomedicals, Illkirch, France), 1 U/μL of Superscript IV Reverse Transcriptase (Invitrogen), 1 U/μL of Recombinant RNasin Ribonuclease Inhibitor (Promega), 5 mM of dithiothreitol (Invitrogen), 5 mM of reverse transcriptase buffer (Invitrogen).

    Article Title: Anti-obesity Effect of Catechin Added Functional Kimchi and its Metabolites
    Article Snippet: .. Page 4/17 Measurement of mRNA expression levels The total RNA of liver and epididymal tissues was isolated using TRIzol reagent according to the manufacturer's instructions (Invitrogen, Carlsbad, CA, USA). cDNA was obtained using oligo dT18 (Invitrogen), reverse transcriptase buffer (Invitrogen), dNTPs (Invitrogen), reverse transcriptase (Invitrogen), and RNase inhibitor (Invitrogen). cDNA synthesis of isolated total RNA was performed using a 2720 thermal cycler (Applied Biosystems, Foster, CA, USA). .. The synthesized cDNA was ampli ed using a thermal cycler Bio-Rad CFX-96 real-time system (Bio-Rad, Hercules, CA, USA).

    Article Title: Does non-steroidal anti-inflammatory drugs-related preemptive analgesia modulate SOCS3/IL-6 pathway in oral surgery?
    Article Snippet: The dental surgeon performs several surgical procedures that tend to significantly affect the patient's quality of life in the postoperative period; surgery of the mandibular third molars is a standard procedure that substantially involves the quality of life of patients in the postoperative period, mainly on the third day after surgery, due to the intensity of pain felt and inflammatory events leading to edema and trismus caused by this type of surgical intervention (Benediktsdóttir et al. 2004; Costa et al. 2015).. In this context, preemptive analgesia represents an antinociceptive treatment that prevents establishing an altered afferent input process, which would amplify postoperative pain (Ong et al. 2005).. Preemptive analgesia in mandibular third molar extractions is a pharmacological strategy that provides greater patient comfort and reduces the intake of analgesic medications for pain control in the postoperative period, reducing the patient's recovery time (Al-Sukhun et al. 2012).

    Article Title: Ethylhexadecyldimethylammonium bromide, a quaternary ammonium compound, controls inflammatory response through NRF2 pathway in a human immortalized keratinocyte cell line.
    Article Snippet: .. 500 ng total RNA were reverse-transcribed using the thermal cycler T100 (Bio-Rad) in a total-20 μL reaction mixture: 2.5 μM of Oligo (dT) (Promega, Charbonnières-les-Bains, France), 0.5 mM of deoxy-nucleotide triphosphate (dNTP; MP Biomedicals, Illkirch, France), 1 U/μL of Superscript IV Reverse Transcriptase (Invitrogen), 1 U/μL of Recombinant RNasin Ribonuclease Inhibitor (Promega), 5 mM of dithiothreitol (Invitrogen), 5 mM of reverse transcriptase buffer (Invitrogen). .. 1/20th of each generated cDNA was used for real-time PCR analysis, which was performed using the SYBR Green technology (Sso Advanced universal SYBR Green SuperMix, Bio-Rad) on thermal cycler CFX384 (Bio-Rad).

    Article Title: α-Pinene Improves Follicle Morphology and Increases the Expression of mRNA for Nuclear Factor Erythroid 2-Related Factor 2 and Peroxiredoxin 6 in Bovine Ovarian Tissues Cultured In Vitro
    Article Snippet: .. Reverse transcription was performed in a total volume of 20 μL composed of 4 μL reverse transcriptase buffer (Invitrogen), 10 μL of RNA sample, eight units of RNAse, 150 units of reverse transcriptase (Superscript III, Invitrogen, São Paulo, Brazil), 10 mM dithiothreitol, 0.036 U random primers, and 0.5 mM of each dNTP (Invitrogen). ..

    Article Title: Antiobesity effect and metabolite analysis of catechin functional kimchi
    Article Snippet: .. The total RNA of liver and epididymal tissues was isolated using TRIzol reagent according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA, USA). cDNA was obtained using oligo dT18 (Invitrogen), reverse transcriptase buffer (Invitrogen), dNTPs (Invitrogen), reverse transcriptase (Invitrogen), and RNase inhibitor (Invitrogen). cDNA synthesis of isolated total RNA was performed using a 2720 thermal cycler (Applied Biosystems, Foster, CA, USA). .. The synthesized cDNA was amplified using a thermal cycler Bio-Rad CFX-96 real-time system (Bio-Rad, Hercules, CA, USA).

    Recombinant:

    Article Title: Ethylhexadecyldimethylammonium bromide, a quaternary ammonium compound, controls inflammatory response through NRF2 pathway in a human immortalized keratinocyte cell line
    Article Snippet: Total RNA isolation was performed with PureLink RNA Mini (Invitrogen). .. 500 ng total RNA were reverse-transcribed using the thermal cycler T100 (Bio-Rad) in a total-20 μL reaction mixture: 2.5 μM of Oligo (dT) (Promega, Charbonnières-les-Bains, France), 0.5 mM of deoxy-nucleotide triphosphate (dNTP; MP Biomedicals, Illkirch, France), 1 U/μL of Superscript IV Reverse Transcriptase (Invitrogen), 1 U/μL of Recombinant RNasin Ribonuclease Inhibitor (Promega), 5 mM of dithiothreitol (Invitrogen), 5 mM of reverse transcriptase buffer (Invitrogen). .. 500 ng total RNA were reverse-transcribed using the thermal cycler T100 (Bio-Rad) in a total-20 μL reaction mixture: 2.5 μM of Oligo (dT) (Promega, Charbonnières-les-Bains, France), 0.5 mM of deoxy-nucleotide triphosphate (dNTP; MP Biomedicals, Illkirch, France), 1 U/μL of Superscript IV Reverse Transcriptase (Invitrogen), 1 U/μL of Recombinant RNasin Ribonuclease Inhibitor (Promega), 5 mM of dithiothreitol (Invitrogen), 5 mM of reverse transcriptase buffer (Invitrogen).

    Article Title: Ethylhexadecyldimethylammonium bromide, a quaternary ammonium compound, controls inflammatory response through NRF2 pathway in a human immortalized keratinocyte cell line.
    Article Snippet: .. 500 ng total RNA were reverse-transcribed using the thermal cycler T100 (Bio-Rad) in a total-20 μL reaction mixture: 2.5 μM of Oligo (dT) (Promega, Charbonnières-les-Bains, France), 0.5 mM of deoxy-nucleotide triphosphate (dNTP; MP Biomedicals, Illkirch, France), 1 U/μL of Superscript IV Reverse Transcriptase (Invitrogen), 1 U/μL of Recombinant RNasin Ribonuclease Inhibitor (Promega), 5 mM of dithiothreitol (Invitrogen), 5 mM of reverse transcriptase buffer (Invitrogen). .. 1/20th of each generated cDNA was used for real-time PCR analysis, which was performed using the SYBR Green technology (Sso Advanced universal SYBR Green SuperMix, Bio-Rad) on thermal cycler CFX384 (Bio-Rad).

    Expressing:

    Article Title: Anti-obesity Effect of Catechin Added Functional Kimchi and its Metabolites
    Article Snippet: .. Page 4/17 Measurement of mRNA expression levels The total RNA of liver and epididymal tissues was isolated using TRIzol reagent according to the manufacturer's instructions (Invitrogen, Carlsbad, CA, USA). cDNA was obtained using oligo dT18 (Invitrogen), reverse transcriptase buffer (Invitrogen), dNTPs (Invitrogen), reverse transcriptase (Invitrogen), and RNase inhibitor (Invitrogen). cDNA synthesis of isolated total RNA was performed using a 2720 thermal cycler (Applied Biosystems, Foster, CA, USA). .. The synthesized cDNA was ampli ed using a thermal cycler Bio-Rad CFX-96 real-time system (Bio-Rad, Hercules, CA, USA).

    Isolation:

    Article Title: Anti-obesity Effect of Catechin Added Functional Kimchi and its Metabolites
    Article Snippet: .. Page 4/17 Measurement of mRNA expression levels The total RNA of liver and epididymal tissues was isolated using TRIzol reagent according to the manufacturer's instructions (Invitrogen, Carlsbad, CA, USA). cDNA was obtained using oligo dT18 (Invitrogen), reverse transcriptase buffer (Invitrogen), dNTPs (Invitrogen), reverse transcriptase (Invitrogen), and RNase inhibitor (Invitrogen). cDNA synthesis of isolated total RNA was performed using a 2720 thermal cycler (Applied Biosystems, Foster, CA, USA). .. The synthesized cDNA was ampli ed using a thermal cycler Bio-Rad CFX-96 real-time system (Bio-Rad, Hercules, CA, USA).

    Article Title: Antiobesity effect and metabolite analysis of catechin functional kimchi
    Article Snippet: .. The total RNA of liver and epididymal tissues was isolated using TRIzol reagent according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA, USA). cDNA was obtained using oligo dT18 (Invitrogen), reverse transcriptase buffer (Invitrogen), dNTPs (Invitrogen), reverse transcriptase (Invitrogen), and RNase inhibitor (Invitrogen). cDNA synthesis of isolated total RNA was performed using a 2720 thermal cycler (Applied Biosystems, Foster, CA, USA). .. The synthesized cDNA was amplified using a thermal cycler Bio-Rad CFX-96 real-time system (Bio-Rad, Hercules, CA, USA).

    cDNA Synthesis:

    Article Title: Antiobesity effect and metabolite analysis of catechin functional kimchi
    Article Snippet: .. The total RNA of liver and epididymal tissues was isolated using TRIzol reagent according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA, USA). cDNA was obtained using oligo dT18 (Invitrogen), reverse transcriptase buffer (Invitrogen), dNTPs (Invitrogen), reverse transcriptase (Invitrogen), and RNase inhibitor (Invitrogen). cDNA synthesis of isolated total RNA was performed using a 2720 thermal cycler (Applied Biosystems, Foster, CA, USA). .. The synthesized cDNA was amplified using a thermal cycler Bio-Rad CFX-96 real-time system (Bio-Rad, Hercules, CA, USA).



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    Image Search Results


    Journal: iScience

    Article Title: Time course transcriptomic profiling suggests Crp/Fnr transcriptional regulation of nosZ gene in a N 2 O-reducing thermophile

    doi: 10.1016/j.isci.2024.111074

    Figure Lengend Snippet:

    Article Snippet: Maxima H Minus Reverse Transcriptase (200U/μL) with 5×RT Buffer , ThermoFisher , Cat# EP0751.

    Techniques: Virus, Recombinant, Reverse Transcription, Isolation, Control, Sequencing, Mass Spectrometry, Software, In Silico, Targeted Proteomics