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5× reverse-transcriptase (rt) buffer  (Thermo Fisher)


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    Thermo Fisher 5× reverse-transcriptase (rt) buffer
    5× Reverse Transcriptase (Rt) Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/5%C3%97+reverse+transcriptase+(rt)+buffer/pmc06807149-93-67-70
    Average 90 stars, based on 1 article reviews
    5× reverse-transcriptase (rt) buffer - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    cDNA Synthesis:

    Article Title: Somatic ‘Soluble’ Adenylyl Cyclase Isoforms Are Unaffected in Sacy tm1Lex /Sacy tm1Lex ‘Knockout’ Mice
    Article Snippet: .. An equal volume of cDNA Synthesis Buffer was added, yielding final concentrations of 1× Reverse Transcriptase Buffer (Invitrogen), 10 mM DTT, 125 mM MgCl 2 , 40U RNaseOUT, 200U SuperScript III Reverse Trancriptase, and the reaction was incubated for 60 minutes at 50°C. ..

    Article Title: The Novel Oral BET-CBP/p300 Dual Inhibitor NEO2734 Is Highly Effective in Eradicating Acute Myeloid Leukemia Blasts and Stem/Progenitor Cells
    Article Snippet: Concentration and quality of the RNA was determined with a NanoDrop 1000 (ThermoFisher Scientific). .. For cDNA synthesis, 1000 ng of RNA was incubated with reverse transcriptase buffer (Invitrogen), 1 mM dithiothreitol (Invitrogen), 1 mM deoxyribonucleotide triphosphates (Roche), 1.89 μg random hexamer primers (Roche), 300 U/μL moloney-murine leukemia virus reverse transcriptase (Invitrogen), 40 U/μL RNasin (Sphaero Q) in nuclease-free water, and incubated for 2 hours at 37°C and 10 minutes at 65°C. .. Quantitative real-time polymerase chain reactions (Q-RT-PCR) reactions were performed in duplicate with 2 μL cDNA (1 μg), 10 μL TaqMan Gene Expression Master Mix (Applied Biosystems), 1 μL Bcl2 (Hs00608023_m1) or c-Myc (Hs00153408_m1) 20x TaqMan Gene Expression Assay probe mix (Applied Biosystems) and 7 μL nuclease-free water.

    Reverse Transcription:

    Article Title: Somatic ‘Soluble’ Adenylyl Cyclase Isoforms Are Unaffected in Sacy tm1Lex /Sacy tm1Lex ‘Knockout’ Mice
    Article Snippet: .. An equal volume of cDNA Synthesis Buffer was added, yielding final concentrations of 1× Reverse Transcriptase Buffer (Invitrogen), 10 mM DTT, 125 mM MgCl 2 , 40U RNaseOUT, 200U SuperScript III Reverse Trancriptase, and the reaction was incubated for 60 minutes at 50°C. ..

    Article Title: Ex Vivo Analysis of Human Memory B Lymphocytes Specific for A and B Influenza Hemagglutinin by Polychromatic Flow-Cytometry
    Article Snippet: .. The mix contains 150 ng of random hexamer primers (Roche), 0.5% v/v Igepal (SIGMA CA-630), 1 U RNAse Inhibitor (QIAGEN), 4 U RNAsin (Promega), 10 mM DTT (Invitrogen), 0.5 mM dNTP mix (Roche), 50 U SuperScript III Reverse Transcriptase (Invitrogen), 5× Reverse Transcriptase Buffer (Invitrogen) diluted in nuclease free water (Invitrogen). ..

    Article Title: Distinct expression patterns of the E3 ligase SIAH-1 and its partner Kid/KIF22 in normal tissues and in the breast tumoral processes
    Article Snippet: .. One microgram of total RNA was reverse-transcribed in a final volume of 20 μL containing 1 × reverse transcriptase buffer (Invitrogen), 1.25 mM dNTPs (Quantum Biotechnologies) 10 mM DTT (Invitrogen), 5 ng/μL random hexamers (Roche), 1 U/μL RNAse inhibitor and 10 U/μL M-MLV reverse transcriptase (Invitrogen). ..

    Article Title: Induction of viral mimicry upon loss of DHX9 and ADAR1 in breast cancer cells
    Article Snippet: Ribosomal RNA was removed by an RNase-H method using RiboErase kit (Kapa Biosystems). .. After rRNA depletion, the remaining RNA was then fragmented in reverse transcriptase buffer (Life Technologies) by heating to 94 degrees for 8 minutes. .. The RNA was reverse transcribed to yield cDNA using SuperScript III RT and random hexamers (Life Technologies) per manufacturer’s instructions.

    Article Title: The Novel Oral BET-CBP/p300 Dual Inhibitor NEO2734 Is Highly Effective in Eradicating Acute Myeloid Leukemia Blasts and Stem/Progenitor Cells
    Article Snippet: Concentration and quality of the RNA was determined with a NanoDrop 1000 (ThermoFisher Scientific). .. For cDNA synthesis, 1000 ng of RNA was incubated with reverse transcriptase buffer (Invitrogen), 1 mM dithiothreitol (Invitrogen), 1 mM deoxyribonucleotide triphosphates (Roche), 1.89 μg random hexamer primers (Roche), 300 U/μL moloney-murine leukemia virus reverse transcriptase (Invitrogen), 40 U/μL RNasin (Sphaero Q) in nuclease-free water, and incubated for 2 hours at 37°C and 10 minutes at 65°C. .. Quantitative real-time polymerase chain reactions (Q-RT-PCR) reactions were performed in duplicate with 2 μL cDNA (1 μg), 10 μL TaqMan Gene Expression Master Mix (Applied Biosystems), 1 μL Bcl2 (Hs00608023_m1) or c-Myc (Hs00153408_m1) 20x TaqMan Gene Expression Assay probe mix (Applied Biosystems) and 7 μL nuclease-free water.

    Article Title: A semi-synthetic flavonoid from Bauhinia pulchella stem attenuates inflammatory osteolysis in periodontitis in rats: Impact on cytokine levels, oxidative stress, and RANK/RANKL/OPG pathway.
    Article Snippet: Objectives: Many species of the Bauhinia genus have been widely used in folk medicine as analgesic, anti-inflammatory and antioxidant agents. (-)-Fisetinidol palmitate is a semi-syntetic flavonoid obtained from the ethanolic extract of the stem of Bauhinia pulchella.. This study aimed to evaluate the antiresorptive effect of the semi-syntetic (-)-fisetinidol palmitate in ligature-induced periodontitis in rats.. Also, it evaluated the mechanism of action of (-)fisetinidol palmitate and its toxicity.

    Incubation:

    Article Title: Somatic ‘Soluble’ Adenylyl Cyclase Isoforms Are Unaffected in Sacy tm1Lex /Sacy tm1Lex ‘Knockout’ Mice
    Article Snippet: .. An equal volume of cDNA Synthesis Buffer was added, yielding final concentrations of 1× Reverse Transcriptase Buffer (Invitrogen), 10 mM DTT, 125 mM MgCl 2 , 40U RNaseOUT, 200U SuperScript III Reverse Trancriptase, and the reaction was incubated for 60 minutes at 50°C. ..

    Article Title: The Novel Oral BET-CBP/p300 Dual Inhibitor NEO2734 Is Highly Effective in Eradicating Acute Myeloid Leukemia Blasts and Stem/Progenitor Cells
    Article Snippet: Concentration and quality of the RNA was determined with a NanoDrop 1000 (ThermoFisher Scientific). .. For cDNA synthesis, 1000 ng of RNA was incubated with reverse transcriptase buffer (Invitrogen), 1 mM dithiothreitol (Invitrogen), 1 mM deoxyribonucleotide triphosphates (Roche), 1.89 μg random hexamer primers (Roche), 300 U/μL moloney-murine leukemia virus reverse transcriptase (Invitrogen), 40 U/μL RNasin (Sphaero Q) in nuclease-free water, and incubated for 2 hours at 37°C and 10 minutes at 65°C. .. Quantitative real-time polymerase chain reactions (Q-RT-PCR) reactions were performed in duplicate with 2 μL cDNA (1 μg), 10 μL TaqMan Gene Expression Master Mix (Applied Biosystems), 1 μL Bcl2 (Hs00608023_m1) or c-Myc (Hs00153408_m1) 20x TaqMan Gene Expression Assay probe mix (Applied Biosystems) and 7 μL nuclease-free water.

    Random Hexamer:

    Article Title: Ex Vivo Analysis of Human Memory B Lymphocytes Specific for A and B Influenza Hemagglutinin by Polychromatic Flow-Cytometry
    Article Snippet: .. The mix contains 150 ng of random hexamer primers (Roche), 0.5% v/v Igepal (SIGMA CA-630), 1 U RNAse Inhibitor (QIAGEN), 4 U RNAsin (Promega), 10 mM DTT (Invitrogen), 0.5 mM dNTP mix (Roche), 50 U SuperScript III Reverse Transcriptase (Invitrogen), 5× Reverse Transcriptase Buffer (Invitrogen) diluted in nuclease free water (Invitrogen). ..

    Article Title: The Novel Oral BET-CBP/p300 Dual Inhibitor NEO2734 Is Highly Effective in Eradicating Acute Myeloid Leukemia Blasts and Stem/Progenitor Cells
    Article Snippet: Concentration and quality of the RNA was determined with a NanoDrop 1000 (ThermoFisher Scientific). .. For cDNA synthesis, 1000 ng of RNA was incubated with reverse transcriptase buffer (Invitrogen), 1 mM dithiothreitol (Invitrogen), 1 mM deoxyribonucleotide triphosphates (Roche), 1.89 μg random hexamer primers (Roche), 300 U/μL moloney-murine leukemia virus reverse transcriptase (Invitrogen), 40 U/μL RNasin (Sphaero Q) in nuclease-free water, and incubated for 2 hours at 37°C and 10 minutes at 65°C. .. Quantitative real-time polymerase chain reactions (Q-RT-PCR) reactions were performed in duplicate with 2 μL cDNA (1 μg), 10 μL TaqMan Gene Expression Master Mix (Applied Biosystems), 1 μL Bcl2 (Hs00608023_m1) or c-Myc (Hs00153408_m1) 20x TaqMan Gene Expression Assay probe mix (Applied Biosystems) and 7 μL nuclease-free water.

    Virus:

    Article Title: The Novel Oral BET-CBP/p300 Dual Inhibitor NEO2734 Is Highly Effective in Eradicating Acute Myeloid Leukemia Blasts and Stem/Progenitor Cells
    Article Snippet: Concentration and quality of the RNA was determined with a NanoDrop 1000 (ThermoFisher Scientific). .. For cDNA synthesis, 1000 ng of RNA was incubated with reverse transcriptase buffer (Invitrogen), 1 mM dithiothreitol (Invitrogen), 1 mM deoxyribonucleotide triphosphates (Roche), 1.89 μg random hexamer primers (Roche), 300 U/μL moloney-murine leukemia virus reverse transcriptase (Invitrogen), 40 U/μL RNasin (Sphaero Q) in nuclease-free water, and incubated for 2 hours at 37°C and 10 minutes at 65°C. .. Quantitative real-time polymerase chain reactions (Q-RT-PCR) reactions were performed in duplicate with 2 μL cDNA (1 μg), 10 μL TaqMan Gene Expression Master Mix (Applied Biosystems), 1 μL Bcl2 (Hs00608023_m1) or c-Myc (Hs00153408_m1) 20x TaqMan Gene Expression Assay probe mix (Applied Biosystems) and 7 μL nuclease-free water.

    other:

    Article Title: Long-Lasting Inhibitory Effects of Fetal Liver Mesenchymal Stem Cells on T-Lymphocyte Proliferation
    Article Snippet: One microgram of total RNA was incubated for 30 min at 37°C with 0.2 U RQ1 DNAse (Promega, Madison, WI) in reverse transcriptase (RT) buffer (Gibco, Life Technologies) in a final volume of 20 μl.



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    Image Search Results


    Journal: iScience

    Article Title: Time course transcriptomic profiling suggests Crp/Fnr transcriptional regulation of nosZ gene in a N 2 O-reducing thermophile

    doi: 10.1016/j.isci.2024.111074

    Figure Lengend Snippet:

    Article Snippet: Maxima H Minus Reverse Transcriptase (200U/μL) with 5×RT Buffer , ThermoFisher , Cat# EP0751.

    Techniques: Virus, Recombinant, Reverse Transcription, Isolation, Control, Sequencing, Mass Spectrometry, Software, In Silico, Targeted Proteomics