5× reverse-transcriptase (rt) buffer (Thermo Fisher)
90
Structured Review
Thermo Fisher
5× reverse-transcriptase (rt) buffer
5× Reverse Transcriptase (Rt) Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5%C3%97+reverse+transcriptase+(rt)+buffer/pmc06807149-93-67-70
Average 90 stars, based on 1 article reviews
5× Reverse Transcriptase (Rt) Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5%C3%97+reverse+transcriptase+(rt)+buffer/pmc06807149-93-67-70
Average 90 stars, based on 1 article reviews
5× reverse-transcriptase (rt) buffer - by Bioz Stars,
2026-09
90/100 stars
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cDNA Synthesis:Article Title: Somatic ‘Soluble’ Adenylyl Cyclase Isoforms Are Unaffected in Sacy tm1Lex /Sacy tm1Lex ‘Knockout’ Mice Article Snippet: .. An equal volume of cDNA Synthesis Buffer was added, yielding final concentrations of 1× Article Title: The Novel Oral BET-CBP/p300 Dual Inhibitor NEO2734 Is Highly Effective in Eradicating Acute Myeloid Leukemia Blasts and Stem/Progenitor Cells Article Snippet: Concentration and quality of the RNA was determined with a NanoDrop 1000 (ThermoFisher Scientific). .. For cDNA synthesis, 1000 ng of RNA was incubated with reverse Reverse Transcription:Article Title: Somatic ‘Soluble’ Adenylyl Cyclase Isoforms Are Unaffected in Sacy tm1Lex /Sacy tm1Lex ‘Knockout’ Mice Article Snippet: .. An equal volume of cDNA Synthesis Buffer was added, yielding final concentrations of 1× Article Title: Ex Vivo Analysis of Human Memory B Lymphocytes Specific for A and B Influenza Hemagglutinin by Polychromatic Flow-Cytometry Article Snippet: .. The mix contains 150 ng of random hexamer primers (Roche), 0.5% v/v Igepal (SIGMA CA-630), 1 U RNAse Inhibitor (QIAGEN), 4 U RNAsin (Promega), 10 mM DTT (Invitrogen), 0.5 mM dNTP mix (Roche), 50 U SuperScript III Reverse Transcriptase (Invitrogen), 5× Article Title: Distinct expression patterns of the E3 ligase SIAH-1 and its partner Kid/KIF22 in normal tissues and in the breast tumoral processes Article Snippet: .. One microgram of total RNA was reverse-transcribed in a final volume of 20 μL containing 1 × Article Title: Induction of viral mimicry upon loss of DHX9 and ADAR1 in breast cancer cells Article Snippet: Ribosomal RNA was removed by an RNase-H method using RiboErase kit (Kapa Biosystems). .. After rRNA depletion, the remaining RNA was then fragmented in reverse Article Title: The Novel Oral BET-CBP/p300 Dual Inhibitor NEO2734 Is Highly Effective in Eradicating Acute Myeloid Leukemia Blasts and Stem/Progenitor Cells Article Snippet: Concentration and quality of the RNA was determined with a NanoDrop 1000 (ThermoFisher Scientific). .. For cDNA synthesis, 1000 ng of RNA was incubated with reverse Article Title: A semi-synthetic flavonoid from Bauhinia pulchella stem attenuates inflammatory osteolysis in periodontitis in rats: Impact on cytokine levels, oxidative stress, and RANK/RANKL/OPG pathway. Article Snippet: Objectives: Many species of the Bauhinia genus have been widely used in folk medicine as analgesic, anti-inflammatory and antioxidant agents. (-)-Fisetinidol palmitate is a semi-syntetic flavonoid obtained from the ethanolic extract of the stem of Bauhinia pulchella.. This study aimed to evaluate the antiresorptive effect of the semi-syntetic (-)-fisetinidol palmitate in ligature-induced periodontitis in rats.. Also, it evaluated the mechanism of action of (-)fisetinidol palmitate and its toxicity. Incubation:Article Title: Somatic ‘Soluble’ Adenylyl Cyclase Isoforms Are Unaffected in Sacy tm1Lex /Sacy tm1Lex ‘Knockout’ Mice Article Snippet: .. An equal volume of cDNA Synthesis Buffer was added, yielding final concentrations of 1× Article Title: The Novel Oral BET-CBP/p300 Dual Inhibitor NEO2734 Is Highly Effective in Eradicating Acute Myeloid Leukemia Blasts and Stem/Progenitor Cells Article Snippet: Concentration and quality of the RNA was determined with a NanoDrop 1000 (ThermoFisher Scientific). .. For cDNA synthesis, 1000 ng of RNA was incubated with reverse Random Hexamer:Article Title: Ex Vivo Analysis of Human Memory B Lymphocytes Specific for A and B Influenza Hemagglutinin by Polychromatic Flow-Cytometry Article Snippet: .. The mix contains 150 ng of random hexamer primers (Roche), 0.5% v/v Igepal (SIGMA CA-630), 1 U RNAse Inhibitor (QIAGEN), 4 U RNAsin (Promega), 10 mM DTT (Invitrogen), 0.5 mM dNTP mix (Roche), 50 U SuperScript III Reverse Transcriptase (Invitrogen), 5× Article Title: The Novel Oral BET-CBP/p300 Dual Inhibitor NEO2734 Is Highly Effective in Eradicating Acute Myeloid Leukemia Blasts and Stem/Progenitor Cells Article Snippet: Concentration and quality of the RNA was determined with a NanoDrop 1000 (ThermoFisher Scientific). .. For cDNA synthesis, 1000 ng of RNA was incubated with reverse Virus:Article Title: The Novel Oral BET-CBP/p300 Dual Inhibitor NEO2734 Is Highly Effective in Eradicating Acute Myeloid Leukemia Blasts and Stem/Progenitor Cells Article Snippet: Concentration and quality of the RNA was determined with a NanoDrop 1000 (ThermoFisher Scientific). .. For cDNA synthesis, 1000 ng of RNA was incubated with reverse other:Article Title: Long-Lasting Inhibitory Effects of Fetal Liver Mesenchymal Stem Cells on T-Lymphocyte Proliferation Article Snippet: One microgram of total RNA was incubated for 30 min at 37°C with 0.2 U RQ1 DNAse (Promega, Madison, WI) in reverse |
