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Sangon Biotech u6 upstream primer 268
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Related Articles

Transfection:

Article Title: scRNA-seq reveals different cell clusters in the testes of Mongolian cattle and EGR1/FOS/JUN regulation in Sertoli cells.
Article Snippet: The fluorescence signals were captured under a microscope (Nikon). .. Cell transfection and quantitative real-time PCR (qPCR) Three siRNAs for EGR1 (Supplemental_Table_S9) were designed and synthesized (Sangon Biotech, Shanghai, China), and an overexpression plasmid with the CDS (NM_001045875.1) region of EGR1 (pcDNA 3.1-EGR1) was constructed. .. Sertoli cells were incubated in a 6-well plate to grow to 50%~70% confluence and were transfected with EGR1-siRNAs and overexpression plasmids using Lipofectamine 2000 (Invitrogen) in DMEM/F12 medium supplemented with antibiotics according to the manufacturer’s instructions.

Real-time Polymerase Chain Reaction:

Article Title: scRNA-seq reveals different cell clusters in the testes of Mongolian cattle and EGR1/FOS/JUN regulation in Sertoli cells.
Article Snippet: The fluorescence signals were captured under a microscope (Nikon). .. Cell transfection and quantitative real-time PCR (qPCR) Three siRNAs for EGR1 (Supplemental_Table_S9) were designed and synthesized (Sangon Biotech, Shanghai, China), and an overexpression plasmid with the CDS (NM_001045875.1) region of EGR1 (pcDNA 3.1-EGR1) was constructed. .. Sertoli cells were incubated in a 6-well plate to grow to 50%~70% confluence and were transfected with EGR1-siRNAs and overexpression plasmids using Lipofectamine 2000 (Invitrogen) in DMEM/F12 medium supplemented with antibiotics according to the manufacturer’s instructions.

Synthesized:

Article Title: scRNA-seq reveals different cell clusters in the testes of Mongolian cattle and EGR1/FOS/JUN regulation in Sertoli cells.
Article Snippet: The fluorescence signals were captured under a microscope (Nikon). .. Cell transfection and quantitative real-time PCR (qPCR) Three siRNAs for EGR1 (Supplemental_Table_S9) were designed and synthesized (Sangon Biotech, Shanghai, China), and an overexpression plasmid with the CDS (NM_001045875.1) region of EGR1 (pcDNA 3.1-EGR1) was constructed. .. Sertoli cells were incubated in a 6-well plate to grow to 50%~70% confluence and were transfected with EGR1-siRNAs and overexpression plasmids using Lipofectamine 2000 (Invitrogen) in DMEM/F12 medium supplemented with antibiotics according to the manufacturer’s instructions.

Article Title: Cellular and molecular mechanism for reproductive capacity of male Mongolian cattle basing on single-cell sequencing for testis
Article Snippet: The fluorescence signals were captured under microscope (Nikon) .. Three siRNAs for EGR1 (Supplemental_Table_S6) were designed and synthesized (Sangon Biotech, Shanghai, China) and the overexpression plasmid with CDS (NM_001045875.1) region of EGR1 (pcDNA 3.1- EGR1 ) were constructed. .. Sertoli cells were incubated in a 6-well plate to grow at a confluence of 50-70%, which were used to transfect with EGR1 -siRNAs and overexprssion plasmid using Lipofectamine 2000 (Invitrogen) in DMEM/F12 medium with antibiotic free according to the manufacture’s instructions.

Over Expression:

Article Title: scRNA-seq reveals different cell clusters in the testes of Mongolian cattle and EGR1/FOS/JUN regulation in Sertoli cells.
Article Snippet: The fluorescence signals were captured under a microscope (Nikon). .. Cell transfection and quantitative real-time PCR (qPCR) Three siRNAs for EGR1 (Supplemental_Table_S9) were designed and synthesized (Sangon Biotech, Shanghai, China), and an overexpression plasmid with the CDS (NM_001045875.1) region of EGR1 (pcDNA 3.1-EGR1) was constructed. .. Sertoli cells were incubated in a 6-well plate to grow to 50%~70% confluence and were transfected with EGR1-siRNAs and overexpression plasmids using Lipofectamine 2000 (Invitrogen) in DMEM/F12 medium supplemented with antibiotics according to the manufacturer’s instructions.

Article Title: Cellular and molecular mechanism for reproductive capacity of male Mongolian cattle basing on single-cell sequencing for testis
Article Snippet: The fluorescence signals were captured under microscope (Nikon) .. Three siRNAs for EGR1 (Supplemental_Table_S6) were designed and synthesized (Sangon Biotech, Shanghai, China) and the overexpression plasmid with CDS (NM_001045875.1) region of EGR1 (pcDNA 3.1- EGR1 ) were constructed. .. Sertoli cells were incubated in a 6-well plate to grow at a confluence of 50-70%, which were used to transfect with EGR1 -siRNAs and overexprssion plasmid using Lipofectamine 2000 (Invitrogen) in DMEM/F12 medium with antibiotic free according to the manufacture’s instructions.

Plasmid Preparation:

Article Title: scRNA-seq reveals different cell clusters in the testes of Mongolian cattle and EGR1/FOS/JUN regulation in Sertoli cells.
Article Snippet: The fluorescence signals were captured under a microscope (Nikon). .. Cell transfection and quantitative real-time PCR (qPCR) Three siRNAs for EGR1 (Supplemental_Table_S9) were designed and synthesized (Sangon Biotech, Shanghai, China), and an overexpression plasmid with the CDS (NM_001045875.1) region of EGR1 (pcDNA 3.1-EGR1) was constructed. .. Sertoli cells were incubated in a 6-well plate to grow to 50%~70% confluence and were transfected with EGR1-siRNAs and overexpression plasmids using Lipofectamine 2000 (Invitrogen) in DMEM/F12 medium supplemented with antibiotics according to the manufacturer’s instructions.

Article Title: Cellular and molecular mechanism for reproductive capacity of male Mongolian cattle basing on single-cell sequencing for testis
Article Snippet: The fluorescence signals were captured under microscope (Nikon) .. Three siRNAs for EGR1 (Supplemental_Table_S6) were designed and synthesized (Sangon Biotech, Shanghai, China) and the overexpression plasmid with CDS (NM_001045875.1) region of EGR1 (pcDNA 3.1- EGR1 ) were constructed. .. Sertoli cells were incubated in a 6-well plate to grow at a confluence of 50-70%, which were used to transfect with EGR1 -siRNAs and overexprssion plasmid using Lipofectamine 2000 (Invitrogen) in DMEM/F12 medium with antibiotic free according to the manufacture’s instructions.

Construct:

Article Title: scRNA-seq reveals different cell clusters in the testes of Mongolian cattle and EGR1/FOS/JUN regulation in Sertoli cells.
Article Snippet: The fluorescence signals were captured under a microscope (Nikon). .. Cell transfection and quantitative real-time PCR (qPCR) Three siRNAs for EGR1 (Supplemental_Table_S9) were designed and synthesized (Sangon Biotech, Shanghai, China), and an overexpression plasmid with the CDS (NM_001045875.1) region of EGR1 (pcDNA 3.1-EGR1) was constructed. .. Sertoli cells were incubated in a 6-well plate to grow to 50%~70% confluence and were transfected with EGR1-siRNAs and overexpression plasmids using Lipofectamine 2000 (Invitrogen) in DMEM/F12 medium supplemented with antibiotics according to the manufacturer’s instructions.

Article Title: Cellular and molecular mechanism for reproductive capacity of male Mongolian cattle basing on single-cell sequencing for testis
Article Snippet: The fluorescence signals were captured under microscope (Nikon) .. Three siRNAs for EGR1 (Supplemental_Table_S6) were designed and synthesized (Sangon Biotech, Shanghai, China) and the overexpression plasmid with CDS (NM_001045875.1) region of EGR1 (pcDNA 3.1- EGR1 ) were constructed. .. Sertoli cells were incubated in a 6-well plate to grow at a confluence of 50-70%, which were used to transfect with EGR1 -siRNAs and overexprssion plasmid using Lipofectamine 2000 (Invitrogen) in DMEM/F12 medium with antibiotic free according to the manufacture’s instructions.

other:

Article Title: LncRNA 1500026H17Rik knockdown ameliorates high glucose-induced mouse podocyte injuries through the miR-205-5p/EGR1 pathway.
Article Snippet: Background Podocyte injuries and dysfunctions contribute to the development of diabetic nephropathy (DN).. This study aimed to investigate the role and novel mechanism of lncRNA 1500026H17Rik in high glucose (HG)-treated podocytes.. Methods DN mice were induced by streptozotocin, and DN in vitro models were constructed in mouse podocytes treated with HG.

Article Title: Gene therapy targeting miR-212-3p exerts therapeutic effects on MAFLD similar to those of exercise
Article Snippet: The liver cancer cells (HepG2) were plated in six-well plates in antibiotic-free medium for 24 h prior to transfection and transfected at 70% confluency for 48 h using Lipofectamine 2000 reagent (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's instructions.

Article Title: Analysis and estimates of the attributable risk for environmental and genetic risk factors in gastric cancer in a Chinese population.
Article Snippet: This article was downloaded by: [Marshall University] On: 23 May 2013, At: 01:49 Publisher: Taylor & Francis Informa Ltd Registered in England and Wales Registered Number: 1072954 Registered office: Mortimer House, 37-41 Mortimer Street, London W1T 3JH, UK Journal of Toxicology and Environmental Health, Part A: Current Issues Publication details, including instructions for authors and subscription information: http://www.tandfonline.com/loi/uteh20 Analysis and Estimates of the Attributable Risk for Environmental and Genetic Risk Factors in Gastric Cancer in a Chinese Population Xiaobing Shen a , Jiao Zhang b , Yingying Yan a , Yufeng Yang a , Gang Fu a & Yuepu Pu a a School of Public Health, Southeast University, Nanjing, China b School of Clinical Medicine, Southeast University, Nanjing, China Published online: 01 Jun 2009.. To cite this article: Xiaobing Shen , Jiao Zhang , Yingying Yan , Yufeng Yang , Gang Fu & Yuepu Pu (2009): Analysis and Estimates of the Attributable Risk for Environmental and Genetic Risk Factors in Gastric Cancer in a Chinese Population, Journal of Toxicology and Environmental Health, Part A: Current Issues, 72:11-12, 759-766 To link to this article: http://dx.doi.org/10.1080/15287390902841599 PLEASE SCROLL DOWN FOR ARTICLE Full terms and conditions of use: http://www.tandfonline.com/page/terms-and-conditions This article may be used for research, teaching, and private study purposes.. Any substantial or systematic reproduction, redistribution, reselling, loan, sub-licensing, systematic supply, or distribution in any form to anyone is expressly forbidden.

Nucleic Acid Electrophoresis:

Article Title: Screening for and validation of a hepatic fibrosis-related pathway induced by insulin-like growth factor-binding protein-related protein 1
Article Snippet: Objective Our previous studies characterized insulin-like growth factor-binding protein-related protein 1 (IGFBPrP1) as a molecule that promotes hepatic fibrogenesis, but its mechanism has not been fully elucidated.. Here, we have investigated the effect of IGFBPrP1 on gene expression in the hepatic fibrosis-related pathway.. Materials and methods Sprague–Dawley rats received injections of an adenovirus carrying IGFBPrP1 or EGFP cDNA into their tail veins.

Western Blot:

Article Title: Screening for and validation of a hepatic fibrosis-related pathway induced by insulin-like growth factor-binding protein-related protein 1
Article Snippet: Objective Our previous studies characterized insulin-like growth factor-binding protein-related protein 1 (IGFBPrP1) as a molecule that promotes hepatic fibrogenesis, but its mechanism has not been fully elucidated.. Here, we have investigated the effect of IGFBPrP1 on gene expression in the hepatic fibrosis-related pathway.. Materials and methods Sprague–Dawley rats received injections of an adenovirus carrying IGFBPrP1 or EGFP cDNA into their tail veins.



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Image Search Results


The immunological activity of antibodies in rabbit Sera immunized with recombinant adenovirus and protein vaccines. (a) Flow chart of immunization and challenge plan for New Zealand rabbits ( n = 3, respectively). (b) Detection of specific antibody titers at the immune endpoint in the serum of rabbits immunized with different vaccine groups. (c) The concentrations of interleukin‐4 (IL‐4), interleukin‐17A (IL‐17A), and interferon‐gamma (IFN‐γ) in the sera of each vaccine immunization group. (d) The antigen sensitivity of antibodies in each vaccine group and the detection of T.pallidum lysate. (e) Detection of the effects of different sera on the adhesion of T.pallidum to cells by indirect immunofluorescence. (f) Detection of the effects of antisera from each group on the adhesion of T.pallidum to cells by dark‐field microscopy. (g) Bactercidal of antibodies from different groups on the growth of T.pallidum cultivation in vitro. (h) The phagocytosis rate of each group was statistically analyzed according to the number of T.pallidum phagocytized by macrophage and separate tests were conducted on the Nichols reference strain and clinical strains. n.s.: no significance.

Journal: Advanced Science

Article Title: An Adenoviral‐Vectored Tp0326 Vaccine Elicits Robust Functional Antibodies to Prevent Treponema pallidum Dissemination in a Rabbit Model

doi: 10.1002/advs.78078

Figure Lengend Snippet: The immunological activity of antibodies in rabbit Sera immunized with recombinant adenovirus and protein vaccines. (a) Flow chart of immunization and challenge plan for New Zealand rabbits ( n = 3, respectively). (b) Detection of specific antibody titers at the immune endpoint in the serum of rabbits immunized with different vaccine groups. (c) The concentrations of interleukin‐4 (IL‐4), interleukin‐17A (IL‐17A), and interferon‐gamma (IFN‐γ) in the sera of each vaccine immunization group. (d) The antigen sensitivity of antibodies in each vaccine group and the detection of T.pallidum lysate. (e) Detection of the effects of different sera on the adhesion of T.pallidum to cells by indirect immunofluorescence. (f) Detection of the effects of antisera from each group on the adhesion of T.pallidum to cells by dark‐field microscopy. (g) Bactercidal of antibodies from different groups on the growth of T.pallidum cultivation in vitro. (h) The phagocytosis rate of each group was statistically analyzed according to the number of T.pallidum phagocytized by macrophage and separate tests were conducted on the Nichols reference strain and clinical strains. n.s.: no significance.

Article Snippet: ELISA kits for Mouse IL‐4, Mouse IL‐17a, Mouse IFN‐γ, Mouse IFN‐γ were procured from RaybioTech Co., Ltd. (China).

Techniques: Activity Assay, Recombinant, Vaccines, Sensitivity, Immunofluorescence, Microscopy, In Vitro

Protective immune responses in rabbits immunized via different routes with a recombinant adenovirus vaccine against T.pallidum following a low‐dose challenge (1 × 10 2 T. pallidum per site). (a) Flow chart of immunization, challenge, and RIT plan for New Zealand rabbits ( n = 3, respectively). (b) Detection of specific antibody titers at the immune endpoint in the serum of rabbits immunized with different vaccine groups. (c) The concentrations of interleukin‐4 (IL‐4), interleukin‐17A (IL‐17A), and interferon‐gamma (IFN‐γ) in the sera of each vaccine immunization group. (d) Changes in the diameter of chancres following intradermal T.pallidum challenge in rabbits (X = 8/rabbit, respectively). (e) Specific and non‐specific antibodies induced by T.pallidum infection were detected using the T.pallidum particle agglutination assay (TPPA) and the Rapid Plasma Reagin (RPR) test, respectively. (f) The polA gene was amplified by quantitative polymerase chain reaction (qPCR) to measure the T.pallidum burden in the skin at the local site. n.s.: no significance.

Journal: Advanced Science

Article Title: An Adenoviral‐Vectored Tp0326 Vaccine Elicits Robust Functional Antibodies to Prevent Treponema pallidum Dissemination in a Rabbit Model

doi: 10.1002/advs.78078

Figure Lengend Snippet: Protective immune responses in rabbits immunized via different routes with a recombinant adenovirus vaccine against T.pallidum following a low‐dose challenge (1 × 10 2 T. pallidum per site). (a) Flow chart of immunization, challenge, and RIT plan for New Zealand rabbits ( n = 3, respectively). (b) Detection of specific antibody titers at the immune endpoint in the serum of rabbits immunized with different vaccine groups. (c) The concentrations of interleukin‐4 (IL‐4), interleukin‐17A (IL‐17A), and interferon‐gamma (IFN‐γ) in the sera of each vaccine immunization group. (d) Changes in the diameter of chancres following intradermal T.pallidum challenge in rabbits (X = 8/rabbit, respectively). (e) Specific and non‐specific antibodies induced by T.pallidum infection were detected using the T.pallidum particle agglutination assay (TPPA) and the Rapid Plasma Reagin (RPR) test, respectively. (f) The polA gene was amplified by quantitative polymerase chain reaction (qPCR) to measure the T.pallidum burden in the skin at the local site. n.s.: no significance.

Article Snippet: ELISA kits for Mouse IL‐4, Mouse IL‐17a, Mouse IFN‐γ, Mouse IFN‐γ were procured from RaybioTech Co., Ltd. (China).

Techniques: Recombinant, Infection, Agglutination, Clinical Proteomics, Amplification, Real-time Polymerase Chain Reaction

(A) Serum Ang II levels in Control, CKD, SUS and CKD-SUS mice (n=10 per group).(B-C) Quantification and representative images of fluorescence accumulation in the CeA (n = 5; scale bars: left 100 μm and right 50 μm).(D) Representative immunofluorescence images of AT1R (red) and c-Fos (white) expression in CeA sections, with DAPI nuclear staining (blue) (scale bar: 50 μm).(E) Representative co-localization curve corresponding to the region indicated by the green arrow (pointing) in panel (D).Data are presented as mean ± SEM. Statistics used one-way analysis of variance followed by Bonferroni post hoc tests. *p < 0.05, **p < 0.01.

Journal: bioRxiv

Article Title: Chronic kidney disease promotes anxiety susceptibility through an angiotensin II–central amygdala axis

doi: 10.64898/2026.08.16.744184

Figure Lengend Snippet: (A) Serum Ang II levels in Control, CKD, SUS and CKD-SUS mice (n=10 per group).(B-C) Quantification and representative images of fluorescence accumulation in the CeA (n = 5; scale bars: left 100 μm and right 50 μm).(D) Representative immunofluorescence images of AT1R (red) and c-Fos (white) expression in CeA sections, with DAPI nuclear staining (blue) (scale bar: 50 μm).(E) Representative co-localization curve corresponding to the region indicated by the green arrow (pointing) in panel (D).Data are presented as mean ± SEM. Statistics used one-way analysis of variance followed by Bonferroni post hoc tests. *p < 0.05, **p < 0.01.

Article Snippet: Serum and CSF Ang II concentrations were measured using a Mouse Angiotensin II ELISA Kit (RayBiotech, Cat. No. EIA-ANGII, Guangzhou, China).

Techniques: Control, Fluorescence, Immunofluorescence, Expressing, Staining

(A) Cerebrospinal fluid (CSF) Ang II levels. Due to limited CSF volume, each data point represents pooled samples from 3–5 mice (detection limit: 0.1 pg/mL).(B) Representative immunofluorescence images of Ang II in the central amygdala (CeA) (scale bars: 100 μm and 50 μm).(C) Quantification of Ang II fluorescence-positive area in the CeA (n = 4).(D) Representative images of viral transduction and AT1R expression in the CeA (n = 3). Green: virus; red: AT1R; blue: DAPI (scale bar: 100 μm).(E) Quantification of AT1R expression in the CeA after viral transduction (n = 3).Data are presented as mean ± SEM. Statistics used two-tailed unpaired t tests.“ns” indicates no significant difference, **p < 0.01.

Journal: bioRxiv

Article Title: Chronic kidney disease promotes anxiety susceptibility through an angiotensin II–central amygdala axis

doi: 10.64898/2026.08.16.744184

Figure Lengend Snippet: (A) Cerebrospinal fluid (CSF) Ang II levels. Due to limited CSF volume, each data point represents pooled samples from 3–5 mice (detection limit: 0.1 pg/mL).(B) Representative immunofluorescence images of Ang II in the central amygdala (CeA) (scale bars: 100 μm and 50 μm).(C) Quantification of Ang II fluorescence-positive area in the CeA (n = 4).(D) Representative images of viral transduction and AT1R expression in the CeA (n = 3). Green: virus; red: AT1R; blue: DAPI (scale bar: 100 μm).(E) Quantification of AT1R expression in the CeA after viral transduction (n = 3).Data are presented as mean ± SEM. Statistics used two-tailed unpaired t tests.“ns” indicates no significant difference, **p < 0.01.

Article Snippet: Serum and CSF Ang II concentrations were measured using a Mouse Angiotensin II ELISA Kit (RayBiotech, Cat. No. EIA-ANGII, Guangzhou, China).

Techniques: Immunofluorescence, Fluorescence, Transduction, Expressing, Virus, Two Tailed Test