Journal: eLife

Article Title: Murine muscle stem cell response to perturbations of the neuromuscular junction are attenuated with aging

doi: 10.7554/eLife.66749

Figure Lengend Snippet:

Article Snippet: Commercial assay or kit , Satellite Cell Isolation Kit, mouse , Miltenyi Biotec , Miltenyi Biotec 130104268 , .

Techniques: Staining, Activity Assay, Blocking Assay, Plasmid Preparation, Recombinant, Membrane, Hybridization, Sequencing, Cell Isolation, In Situ, Software

Journal: Cell

Article Title: SARS-CoV-2 infection triggers profibrotic macrophage responses and lung fibrosis

doi: 10.1016/j.cell.2021.11.033

Figure Lengend Snippet: CD163 + macrophages accumulate in the lung in severe COVID-19 (A) Overview of study design and analyses. CT, computed tomography; BAL, bronchoalveolar lavage; scRNA-seq, single-cell RNA sequencing; snRNA-seq, single-nucleus RNA sequencing; IHC, immunohistochemistry; IF, immunofluorescence microscopy; MELC, multi-epitope ligand cartography; EM, electron microscopy; VCin, inspiratory vital capacity; PBMC, peripheral blood mononuclear cells; IAV, Influenza A virus. (B) Postmortem analysis of consecutive histological sections of non-COVID-19 (left) and COVID-19 autopsy lung samples (right) by hematoxylin and eosin (H&E; top) and CD68 IHC (bottom). Scale bar, 100 μm. (C) IF of CD68 (green) and CD163 (red) in lung tissue autopsy samples of COVID-19 patients and non-COVID-19 controls. Arrows indicate CD68 + CD163 – macrophages, and arrowheads indicate CD68 + CD163 + macrophages. Scale bar, 20 μm. (D) Quantification of CD68 + macrophage density (left) and the proportion of CD163 + macrophages (right) in lung autopsy samples from fifteen donors (as in C). Mann-Whitney test; ∗ p < 0.05. (E) Representative images of consecutive histological sections of lung autopsy samples. H&E (left), CD68 IHC (middle), and SARS-CoV-2 RNA-FISH (right). Arrowheads indicate SARS-CoV-2 RNA-positive macrophages. Scale bars, 50 μm, 25 μm. RNA-FISH, RNA-fluorescence in situ hybridization. (F) Lung autopsy samples of 9 COVID-19 patients were analyzed by MELC with a panel of 22 markers on 19 fields of view (FOVs). Two-dimensional embedding computed by UMAP on 9,684 computationally identified CD45 positive cells (T cells, CD3 + ; B cells, CD20 + ; NK cells, CD56 + ; neutrophils, MRP14 + /CD66b + ; monocytes, MRP14 + /CCR2 + ; macrophages, MRP14 + /HLA-DR + ). (G) Relative proportion (of total CD45 + cells) of cell types in all 19 FOVs (left), and average cell numbers (summary, right).

Article Snippet: CD66b-PE , Miltenyi Biotec , Cat# 130-122-922, N/A.

Techniques: Computed Tomography, RNA Sequencing Assay, Immunohistochemistry, Immunofluorescence, Microscopy, Electron Microscopy, MANN-WHITNEY, Fluorescence, In Situ Hybridization

Journal: Cell

Article Title: SARS-CoV-2 infection triggers profibrotic macrophage responses and lung fibrosis

doi: 10.1016/j.cell.2021.11.033

Figure Lengend Snippet:

Article Snippet: CD66b-PE , Miltenyi Biotec , Cat# 130-122-922, N/A.

Techniques: Immunohistochemistry, Plasmid Preparation, Recombinant, Staining, Lysis, Protease Inhibitor, Mass Spectrometry, Sequencing, Modification, Bicinchoninic Acid Protein Assay, Enzyme-linked Immunosorbent Assay, Software

Journal: Molecular carcinogenesis

Article Title: CtBP2 Regulates Wnt Signal Through EGR1 to Influence the Proliferation and Apoptosis of DLBCL Cells.

doi: 10.1002/mc.23901

Figure Lengend Snippet: FIGURE 5 | Interfering with EGR1 inhibited the proliferation and cycle acceleration of DLBCL cells and promotes apoptosis. The EGR1 gene in cells was silenced through siRNA transfection. (A) The interference level of EGR1 was measured by western blot analysis. (B) Cell proliferation was detected by CCK8 assay. (C) EdU staining was performed to evaluate the level of cell proliferation; Flow cytometry was conducted to analyze the cell cycle (D) and the level of apoptosis (E). (F) The expressions of CtBP2 and apoptosis‐related proteins were detected by western blot. ***p < 0.001. [Color figure can be viewed at wileyonlinelibrary.com]

Article Snippet: The antibodies utilized in this study included CtBP2 (10346‐1‐AP; Proteintech), EGR‐1 (22008‐1‐AP; Proteintech), Bcl‐2 (12789‐1‐AP; Proteintech), Bax (ab32503; Abcam), Caspase 3 (9961, CST), cleaved‐Caspase 3 (9962, CST), DKK1 (ab307367; Abcam), β‐catenin (ab32572; Abcam) and c‐Myc (ab185656; Abcam).

Techniques: Transfection, Western Blot, CCK-8 Assay, Staining, Flow Cytometry

Journal: Molecular carcinogenesis

Article Title: CtBP2 Regulates Wnt Signal Through EGR1 to Influence the Proliferation and Apoptosis of DLBCL Cells.

doi: 10.1002/mc.23901

Figure Lengend Snippet: FIGURE 6 | Interfere with CtBP2 inhibited the proliferation and cycle acceleration of DLBCL cells and promoted apoptosis through EGR1. The cells were transfected with an Ov‐EGR1 overexpression plasmid. (A) The overexpression level of EGR1 was assessed using Western blot analysis. (B) Cell proliferation was evaluated by CCK‐8 assay. (C) Cell proliferation was measured through EdU staining; Flow cytometry was performed to analyze the cell cycle (D) and the level of apoptosis (E). (F) The expression of apoptosis‐related proteins was detected by western blot assay. ***p < 0.001. [Color figure can be viewed at wileyonlinelibrary.com]

Article Snippet: The antibodies utilized in this study included CtBP2 (10346‐1‐AP; Proteintech), EGR‐1 (22008‐1‐AP; Proteintech), Bcl‐2 (12789‐1‐AP; Proteintech), Bax (ab32503; Abcam), Caspase 3 (9961, CST), cleaved‐Caspase 3 (9962, CST), DKK1 (ab307367; Abcam), β‐catenin (ab32572; Abcam) and c‐Myc (ab185656; Abcam).

Techniques: Transfection, Over Expression, Plasmid Preparation, Western Blot, CCK-8 Assay, Staining, Flow Cytometry, Expressing

Journal: Molecular carcinogenesis

Article Title: CtBP2 Regulates Wnt Signal Through EGR1 to Influence the Proliferation and Apoptosis of DLBCL Cells.

doi: 10.1002/mc.23901

Figure Lengend Snippet: FIGURE 7 | EGR1 may be involved in the regulation of the Wnt/β‐catenin signaling pathway by CtBP2. The expressions of Wnt/β‐catenin signaling‐related proteins DKK1, β‐catenin and c‐MyC were detected by western blot. ***p < 0.001. [Color figure can be viewed at wileyonlinelibrary.com]

Article Snippet: The antibodies utilized in this study included CtBP2 (10346‐1‐AP; Proteintech), EGR‐1 (22008‐1‐AP; Proteintech), Bcl‐2 (12789‐1‐AP; Proteintech), Bax (ab32503; Abcam), Caspase 3 (9961, CST), cleaved‐Caspase 3 (9962, CST), DKK1 (ab307367; Abcam), β‐catenin (ab32572; Abcam) and c‐Myc (ab185656; Abcam).

Techniques: Western Blot