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Structured Review

Sangon Biotech u6 upstream primer 268
U6 Upstream Primer 268, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/268/loop+primer+rt+stem/pm42119634-151-2-9
Average 86 stars, based on 1 article reviews
u6 upstream primer 268 - by Bioz Stars, 2026-11
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Related Articles

Polymerase Chain Reaction:

Article Title: A proof-of-principle study: Species identification based on mitochondrial 12S rRNA gene using QNome nanopore sequencing.
Article Snippet: Accurate species identification can provide crucial evidence to aid criminal investigations and preserve species diversity.. DNA barcoding is an effective tool to ascertain the origin of degraded, trace, or processed samples, and the mitochondrial 12S rRNA gene serving as a potential marker due to its low intraspecific variation and high interspecific variation.. QNome nanopore sequencing, with its flexible read lengths, real-time analysis, and portable device size, offers new means for species identification.

Reverse Transcription:

Article Title: MCAO rat model-guided identification of crucial miRNAs and stroke-related networks via high-throughput sequencing.
Article Snippet: Ischemic stroke is a complex polygenic disorder, in which microRNAs (miRNAs) have been implicated in various physiological and pathological processes.. However, the specific regulatory networks and mechanisms involving brain miRNAs in the development of stroke remain inadequately elucidated.. In this study, a middle cerebral artery occlusion (MCAO) model was established in Sprague-Dawley rats to simulate cerebral ischemia.

Synthesized:

Article Title: Effect of the miR‑26a/b‑5p‑STAT3‑YKL‑40 regulatory axis on proliferation, migration, and invasion of endometrial cancer cells
Article Snippet: .. The U6 primer was designed and synthesized by Sangon Biotech (Shanghai) Co., Ltd. .. The primers for GAPDH, miR‐26a‐5p, miR‐26b‐5p, STAT3, and YKL‐40 were designed and synthesized by Takara Biotechnology (Beijing) Co., Ltd.

Article Title: ace-miR-182-x modulates host immunity and microsporidian proliferation through targeting MMP14 and CRQ genes in Apis cerana cerana.
Article Snippet: As indispensable pollinators in nature, honey bees are of great importance for ecosystem equilibrium and agricultural development.. Apis cerana cerana is a major bee species mainly reared in Asian countries, with high ecological and economic value.. Nosema ceranae is a kind of fungal parasite for bees, causing a chronic disease named bee nosemosis, which results in severe losses for the worldwide apicultural industry.

Sequencing:

Article Title: ace-miR-182-x modulates host immunity and microsporidian proliferation through targeting MMP14 and CRQ genes in Apis cerana cerana.
Article Snippet: As indispensable pollinators in nature, honey bees are of great importance for ecosystem equilibrium and agricultural development.. Apis cerana cerana is a major bee species mainly reared in Asian countries, with high ecological and economic value.. Nosema ceranae is a kind of fungal parasite for bees, causing a chronic disease named bee nosemosis, which results in severe losses for the worldwide apicultural industry.

Article Title: ALKB-1-dependent tRNA methylation is required for efficient paternal mitochondrial elimination
Article Snippet: .. The stem-loop structured tRNA probe modified with m 1 A was obtained from Sangon Biotech (Shanghai, China), and the sequence of the probe was as follow: 5′-CACGGUUCG (m 1 A) UUCAAAG-3′, as previously described . ..

Biomarker Discovery:

Article Title: ace-miR-182-x modulates host immunity and microsporidian proliferation through targeting MMP14 and CRQ genes in Apis cerana cerana.
Article Snippet: As indispensable pollinators in nature, honey bees are of great importance for ecosystem equilibrium and agricultural development.. Apis cerana cerana is a major bee species mainly reared in Asian countries, with high ecological and economic value.. Nosema ceranae is a kind of fungal parasite for bees, causing a chronic disease named bee nosemosis, which results in severe losses for the worldwide apicultural industry.

Expressing:

Article Title: ace-miR-182-x modulates host immunity and microsporidian proliferation through targeting MMP14 and CRQ genes in Apis cerana cerana.
Article Snippet: As indispensable pollinators in nature, honey bees are of great importance for ecosystem equilibrium and agricultural development.. Apis cerana cerana is a major bee species mainly reared in Asian countries, with high ecological and economic value.. Nosema ceranae is a kind of fungal parasite for bees, causing a chronic disease named bee nosemosis, which results in severe losses for the worldwide apicultural industry.

Modification:

Article Title: ALKB-1-dependent tRNA methylation is required for efficient paternal mitochondrial elimination
Article Snippet: .. The stem-loop structured tRNA probe modified with m 1 A was obtained from Sangon Biotech (Shanghai, China), and the sequence of the probe was as follow: 5′-CACGGUUCG (m 1 A) UUCAAAG-3′, as previously described . ..



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Image Search Results


The immunological activity of antibodies in rabbit Sera immunized with recombinant adenovirus and protein vaccines. (a) Flow chart of immunization and challenge plan for New Zealand rabbits ( n = 3, respectively). (b) Detection of specific antibody titers at the immune endpoint in the serum of rabbits immunized with different vaccine groups. (c) The concentrations of interleukin‐4 (IL‐4), interleukin‐17A (IL‐17A), and interferon‐gamma (IFN‐γ) in the sera of each vaccine immunization group. (d) The antigen sensitivity of antibodies in each vaccine group and the detection of T.pallidum lysate. (e) Detection of the effects of different sera on the adhesion of T.pallidum to cells by indirect immunofluorescence. (f) Detection of the effects of antisera from each group on the adhesion of T.pallidum to cells by dark‐field microscopy. (g) Bactercidal of antibodies from different groups on the growth of T.pallidum cultivation in vitro. (h) The phagocytosis rate of each group was statistically analyzed according to the number of T.pallidum phagocytized by macrophage and separate tests were conducted on the Nichols reference strain and clinical strains. n.s.: no significance.

Journal: Advanced Science

Article Title: An Adenoviral‐Vectored Tp0326 Vaccine Elicits Robust Functional Antibodies to Prevent Treponema pallidum Dissemination in a Rabbit Model

doi: 10.1002/advs.78078

Figure Lengend Snippet: The immunological activity of antibodies in rabbit Sera immunized with recombinant adenovirus and protein vaccines. (a) Flow chart of immunization and challenge plan for New Zealand rabbits ( n = 3, respectively). (b) Detection of specific antibody titers at the immune endpoint in the serum of rabbits immunized with different vaccine groups. (c) The concentrations of interleukin‐4 (IL‐4), interleukin‐17A (IL‐17A), and interferon‐gamma (IFN‐γ) in the sera of each vaccine immunization group. (d) The antigen sensitivity of antibodies in each vaccine group and the detection of T.pallidum lysate. (e) Detection of the effects of different sera on the adhesion of T.pallidum to cells by indirect immunofluorescence. (f) Detection of the effects of antisera from each group on the adhesion of T.pallidum to cells by dark‐field microscopy. (g) Bactercidal of antibodies from different groups on the growth of T.pallidum cultivation in vitro. (h) The phagocytosis rate of each group was statistically analyzed according to the number of T.pallidum phagocytized by macrophage and separate tests were conducted on the Nichols reference strain and clinical strains. n.s.: no significance.

Article Snippet: ELISA kits for Mouse IL‐4, Mouse IL‐17a, Mouse IFN‐γ, Mouse IFN‐γ were procured from RaybioTech Co., Ltd. (China).

Techniques: Activity Assay, Recombinant, Vaccines, Sensitivity, Immunofluorescence, Microscopy, In Vitro

Protective immune responses in rabbits immunized via different routes with a recombinant adenovirus vaccine against T.pallidum following a low‐dose challenge (1 × 10 2 T. pallidum per site). (a) Flow chart of immunization, challenge, and RIT plan for New Zealand rabbits ( n = 3, respectively). (b) Detection of specific antibody titers at the immune endpoint in the serum of rabbits immunized with different vaccine groups. (c) The concentrations of interleukin‐4 (IL‐4), interleukin‐17A (IL‐17A), and interferon‐gamma (IFN‐γ) in the sera of each vaccine immunization group. (d) Changes in the diameter of chancres following intradermal T.pallidum challenge in rabbits (X = 8/rabbit, respectively). (e) Specific and non‐specific antibodies induced by T.pallidum infection were detected using the T.pallidum particle agglutination assay (TPPA) and the Rapid Plasma Reagin (RPR) test, respectively. (f) The polA gene was amplified by quantitative polymerase chain reaction (qPCR) to measure the T.pallidum burden in the skin at the local site. n.s.: no significance.

Journal: Advanced Science

Article Title: An Adenoviral‐Vectored Tp0326 Vaccine Elicits Robust Functional Antibodies to Prevent Treponema pallidum Dissemination in a Rabbit Model

doi: 10.1002/advs.78078

Figure Lengend Snippet: Protective immune responses in rabbits immunized via different routes with a recombinant adenovirus vaccine against T.pallidum following a low‐dose challenge (1 × 10 2 T. pallidum per site). (a) Flow chart of immunization, challenge, and RIT plan for New Zealand rabbits ( n = 3, respectively). (b) Detection of specific antibody titers at the immune endpoint in the serum of rabbits immunized with different vaccine groups. (c) The concentrations of interleukin‐4 (IL‐4), interleukin‐17A (IL‐17A), and interferon‐gamma (IFN‐γ) in the sera of each vaccine immunization group. (d) Changes in the diameter of chancres following intradermal T.pallidum challenge in rabbits (X = 8/rabbit, respectively). (e) Specific and non‐specific antibodies induced by T.pallidum infection were detected using the T.pallidum particle agglutination assay (TPPA) and the Rapid Plasma Reagin (RPR) test, respectively. (f) The polA gene was amplified by quantitative polymerase chain reaction (qPCR) to measure the T.pallidum burden in the skin at the local site. n.s.: no significance.

Article Snippet: ELISA kits for Mouse IL‐4, Mouse IL‐17a, Mouse IFN‐γ, Mouse IFN‐γ were procured from RaybioTech Co., Ltd. (China).

Techniques: Recombinant, Infection, Agglutination, Clinical Proteomics, Amplification, Real-time Polymerase Chain Reaction

(A) Serum Ang II levels in Control, CKD, SUS and CKD-SUS mice (n=10 per group).(B-C) Quantification and representative images of fluorescence accumulation in the CeA (n = 5; scale bars: left 100 μm and right 50 μm).(D) Representative immunofluorescence images of AT1R (red) and c-Fos (white) expression in CeA sections, with DAPI nuclear staining (blue) (scale bar: 50 μm).(E) Representative co-localization curve corresponding to the region indicated by the green arrow (pointing) in panel (D).Data are presented as mean ± SEM. Statistics used one-way analysis of variance followed by Bonferroni post hoc tests. *p < 0.05, **p < 0.01.

Journal: bioRxiv

Article Title: Chronic kidney disease promotes anxiety susceptibility through an angiotensin II–central amygdala axis

doi: 10.64898/2026.08.16.744184

Figure Lengend Snippet: (A) Serum Ang II levels in Control, CKD, SUS and CKD-SUS mice (n=10 per group).(B-C) Quantification and representative images of fluorescence accumulation in the CeA (n = 5; scale bars: left 100 μm and right 50 μm).(D) Representative immunofluorescence images of AT1R (red) and c-Fos (white) expression in CeA sections, with DAPI nuclear staining (blue) (scale bar: 50 μm).(E) Representative co-localization curve corresponding to the region indicated by the green arrow (pointing) in panel (D).Data are presented as mean ± SEM. Statistics used one-way analysis of variance followed by Bonferroni post hoc tests. *p < 0.05, **p < 0.01.

Article Snippet: Serum and CSF Ang II concentrations were measured using a Mouse Angiotensin II ELISA Kit (RayBiotech, Cat. No. EIA-ANGII, Guangzhou, China).

Techniques: Control, Fluorescence, Immunofluorescence, Expressing, Staining

(A) Cerebrospinal fluid (CSF) Ang II levels. Due to limited CSF volume, each data point represents pooled samples from 3–5 mice (detection limit: 0.1 pg/mL).(B) Representative immunofluorescence images of Ang II in the central amygdala (CeA) (scale bars: 100 μm and 50 μm).(C) Quantification of Ang II fluorescence-positive area in the CeA (n = 4).(D) Representative images of viral transduction and AT1R expression in the CeA (n = 3). Green: virus; red: AT1R; blue: DAPI (scale bar: 100 μm).(E) Quantification of AT1R expression in the CeA after viral transduction (n = 3).Data are presented as mean ± SEM. Statistics used two-tailed unpaired t tests.“ns” indicates no significant difference, **p < 0.01.

Journal: bioRxiv

Article Title: Chronic kidney disease promotes anxiety susceptibility through an angiotensin II–central amygdala axis

doi: 10.64898/2026.08.16.744184

Figure Lengend Snippet: (A) Cerebrospinal fluid (CSF) Ang II levels. Due to limited CSF volume, each data point represents pooled samples from 3–5 mice (detection limit: 0.1 pg/mL).(B) Representative immunofluorescence images of Ang II in the central amygdala (CeA) (scale bars: 100 μm and 50 μm).(C) Quantification of Ang II fluorescence-positive area in the CeA (n = 4).(D) Representative images of viral transduction and AT1R expression in the CeA (n = 3). Green: virus; red: AT1R; blue: DAPI (scale bar: 100 μm).(E) Quantification of AT1R expression in the CeA after viral transduction (n = 3).Data are presented as mean ± SEM. Statistics used two-tailed unpaired t tests.“ns” indicates no significant difference, **p < 0.01.

Article Snippet: Serum and CSF Ang II concentrations were measured using a Mouse Angiotensin II ELISA Kit (RayBiotech, Cat. No. EIA-ANGII, Guangzhou, China).

Techniques: Immunofluorescence, Fluorescence, Transduction, Expressing, Virus, Two Tailed Test