u133 plus2 microarray (Thermo Fisher)
Structured Review
![Fam170a is located in the nucleus of spermatids at steps 9–13 and has a low level of mRNA in human sperm from individuals with teratozoospermia. ( A , B ) Immunofluorescence staining displayed the localization and distribution of Fam170a protein in mouse and human seminiferous tubules, respectively. Roman numerals indicate the spermatogenic stages of the tubules in WT testicular sections. S: spermatid. The specific stages of mouse tubules were determined according to published criteria [ , ]. ( C ) Schematic summary of Fam170a protein localization during spermiogenesis. ( D ) Three different microarrays captured Fam170a gene transcription levels in sperm samples from normal and teratozoospermia men. The original data source is marked at the top of the histogram. GDS2695 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2695±FAM170A ), GDS2696 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2696+FAM170A ), and <t>GDS2697</t> ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2697+FAM170A ); ** P < .01, * P < .005. The data are displayed as mean ± SEM. A t -test was performed, and false discovery rate (FDR) P -values were shown. ( E ) The relative Fam170a mRNA abundance in sperm samples from normal ( n = 4) and teratozoospermia ( n = 3) men after qRT-PCR analysis. The control data are displayed as mean ± SEM.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_0970/pmc11760970/pmc11760970__gkaf023fig1.jpg)
U133 Plus2 Microarray, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Fam170a deficiency causes male infertility by impairing histone-to-protamine exchange during mouse spermiogenesis"
Article Title: Fam170a deficiency causes male infertility by impairing histone-to-protamine exchange during mouse spermiogenesis
Journal: Nucleic Acids Research
doi: 10.1093/nar/gkaf023
Figure Legend Snippet: Fam170a is located in the nucleus of spermatids at steps 9–13 and has a low level of mRNA in human sperm from individuals with teratozoospermia. ( A , B ) Immunofluorescence staining displayed the localization and distribution of Fam170a protein in mouse and human seminiferous tubules, respectively. Roman numerals indicate the spermatogenic stages of the tubules in WT testicular sections. S: spermatid. The specific stages of mouse tubules were determined according to published criteria [ , ]. ( C ) Schematic summary of Fam170a protein localization during spermiogenesis. ( D ) Three different microarrays captured Fam170a gene transcription levels in sperm samples from normal and teratozoospermia men. The original data source is marked at the top of the histogram. GDS2695 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2695±FAM170A ), GDS2696 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2696+FAM170A ), and GDS2697 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2697+FAM170A ); ** P < .01, * P < .005. The data are displayed as mean ± SEM. A t -test was performed, and false discovery rate (FDR) P -values were shown. ( E ) The relative Fam170a mRNA abundance in sperm samples from normal ( n = 4) and teratozoospermia ( n = 3) men after qRT-PCR analysis. The control data are displayed as mean ± SEM.
Techniques Used: Immunofluorescence, Staining, Quantitative RT-PCR, Control
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Expressing:Article Title: Fam170a deficiency causes male infertility by impairing histone-to-protamine exchange during mouse spermiogenesis. Article Snippet: Illumina Sentrix Expression (HumanRef-8) microbead microarray detection of 10 human male sperm samples (GDS2695, four normal and six teratozoospermia samples) revealed that the Fam170a transcription levels were significantly lower in the teratozoospermia samples than in the normal controls after t -test analysis (Fig. 1 D, P < .01). .. Similarly, the data from the Illumina Sentrix Expression (HumanRef-8) microbead microarray (five normal and eight teratozoospermia samples, GDS2696) and Article Title: Fam170a deficiency causes male infertility by impairing histone-to-protamine exchange during mouse spermiogenesis Article Snippet: Illumina Sentrix Expression (HumanRef-8) microbead microarray detection of 10 human male sperm samples (GDS2695, four normal and six teratozoospermia samples) revealed that the Fam170a transcription levels were significantly lower in the teratozoospermia samples than in the normal controls after t -test analysis (Fig. , P < .01). .. Similarly, the data from the Illumina Sentrix Expression (HumanRef-8) microbead microarray (five normal and eight teratozoospermia samples, GDS2696) and Microarray:Article Title: Fam170a deficiency causes male infertility by impairing histone-to-protamine exchange during mouse spermiogenesis. Article Snippet: Illumina Sentrix Expression (HumanRef-8) microbead microarray detection of 10 human male sperm samples (GDS2695, four normal and six teratozoospermia samples) revealed that the Fam170a transcription levels were significantly lower in the teratozoospermia samples than in the normal controls after t -test analysis (Fig. 1 D, P < .01). .. Similarly, the data from the Illumina Sentrix Expression (HumanRef-8) microbead microarray (five normal and eight teratozoospermia samples, GDS2696) and Article Title: Fam170a deficiency causes male infertility by impairing histone-to-protamine exchange during mouse spermiogenesis Article Snippet: Illumina Sentrix Expression (HumanRef-8) microbead microarray detection of 10 human male sperm samples (GDS2695, four normal and six teratozoospermia samples) revealed that the Fam170a transcription levels were significantly lower in the teratozoospermia samples than in the normal controls after t -test analysis (Fig. , P < .01). .. Similarly, the data from the Illumina Sentrix Expression (HumanRef-8) microbead microarray (five normal and eight teratozoospermia samples, GDS2696) and |