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u133 plus2.0 microarray  (Thermo Fisher)


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    Structured Review

    Thermo Fisher u133 plus2.0 microarray
    U133 Plus2.0 Microarray, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/u133+microarray/pm39804049-74-21-21
    Average 90 stars, based on 1 article reviews
    u133 plus2.0 microarray - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    other:

    Article Title: Sideroflexin family genes were dysregulated and associated with tumor progression in prostate cancers
    Article Snippet: Total RNA was isolated and puri ed using the RNeasy Mini Kit (Qiagen) for cDNA microarray analysis with an Affymetrix Human Genome U133 Plus2.0, as described [26].

    Article Title: Cyclin C promotes development and progression of B-cell acute lymphoblastic leukemia by counteracting p53-mediated stress responses.
    Article Snippet: Expression of CCNC at first diagnosis was determined with Affymetrix U133 plus2.0 gene expression microarrays using probeset 201955_at after vsnrma normalization27.

    Article Title: Pan-Cancer Analysis of PGAM1 and Its Experimental Validation in Uveal Melanoma Progression
    Article Snippet: The on-chip sequencing data (Affymetrix Human Genome U133 Plus2.0 Array platform) and the clinical information files, designated GSE22138 , were also collected from the GEO database ( https://www.ncbi.nlm.nih.gov/geo/ ) download.

    Article Title: Glutaminase inhibitor therapy
    Article Snippet: All wells were then stained with CM-H2DCFDA (5 μM, Life Technologies) for 30 minutes at 37° C., washed with 1×PBS, harvested by trypsinization, and re-suspended in 400 μl phenol-free RPMI medium.

    Article Title: Silencing GRHL3 promotes multiple organ distant metastasis of lung squamous cell carcinoma cells by enhancing SOX2 stability via SIRT1.
    Article Snippet: Aberrant expression of grainyhead-like transcription factor 3 (GRHL3) has been extensively reported in the development and progression of several squamous cell carcinomas, such as cutaneous, head and neck, and esophageal squamous cell carcinoma.. However, the clinical significance and biological roles of GRHL3 in lung squamous cell (LUSC) carcinoma are largely unclear.. Herein, we report that GRHL3 was significantly upregulated in lung squamous epithelium of LUSC tissues, bronchiole, and bronchus.

    Sequencing:

    Article Title: Neoepitope load, T cell signatures and PD-L2 as combined biomarker strategy for response to checkpoint inhibition immunotherapy
    Article Snippet: Blood samples were collected in EDTA tubes, and genomic DNA was extracted using a Tecan automation workstation (Promega). .. Molecular profiling consists of whole-exome sequencing (Illumina platform) and mRNA expression arrays (Human U133 Plus2.0, Affymetrix). ..

    Expressing:

    Article Title: Neoepitope load, T cell signatures and PD-L2 as combined biomarker strategy for response to checkpoint inhibition immunotherapy
    Article Snippet: Blood samples were collected in EDTA tubes, and genomic DNA was extracted using a Tecan automation workstation (Promega). .. Molecular profiling consists of whole-exome sequencing (Illumina platform) and mRNA expression arrays (Human U133 Plus2.0, Affymetrix). ..

    Generated:

    Article Title: Comparison between Three Different Techniques for the Detection of EGFR Mutations in Liquid Biopsies of Patients with Advanced Stage Lung Adenocarcinoma.
    Article Snippet: The ready-to-use Ion AmpliSeq Cancer Hotspot Panel v.2 (CHPv2 250 amplicons) (ThermoFisher Scientific, Waltham, MA, USA) was used to evaluate the mutational status of approximately 2800 COSMIC (catalogue of somatic mutation in cancer) mutations from 50 oncogenes and tumour suppressor genes. .. Libraries were generated starting from 10 ng of input ctDNA with the Ion AmpliSeq Library Kit Plus2.0 and barcoded with Ion Xpress Barcode Adapters (Life Technologies). ..

    Gene Expression:

    Article Title: The CLCA1/TMEM16A/Cl – current axis associates with H 2 S deficiency in diabetic kidney injury
    Article Snippet: .. Kidney biopsy tissue procurement and gene expression profiling were performed on GeneChip Array Human Genome series U133A and Plus 2.0 (Affymetrix) as described previously ( – ). .. Affymetrix image files were obtained, processed, normalized, and batch corrected as described previously.



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    Fam170a is located in the nucleus of spermatids at steps 9–13 and has a low level of mRNA in human sperm from individuals with teratozoospermia. ( A , B ) Immunofluorescence staining displayed the localization and distribution of Fam170a protein in mouse and human seminiferous tubules, respectively. Roman numerals indicate the spermatogenic stages of the tubules in WT testicular sections. S: spermatid. The specific stages of mouse tubules were determined according to published criteria [ , ]. ( C ) Schematic summary of Fam170a protein localization during spermiogenesis. ( D ) Three different microarrays captured Fam170a gene transcription levels in sperm samples from normal and teratozoospermia men. The original data source is marked at the top of the histogram. GDS2695 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2695±FAM170A ), GDS2696 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2696+FAM170A ), and <t>GDS2697</t> ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2697+FAM170A ); ** P < .01, * P < .005. The data are displayed as mean ± SEM. A t -test was performed, and false discovery rate (FDR) P -values were shown. ( E ) The relative Fam170a mRNA abundance in sperm samples from normal ( n = 4) and teratozoospermia ( n = 3) men after qRT-PCR analysis. The control data are displayed as mean ± SEM.
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    Fam170a is located in the nucleus of spermatids at steps 9–13 and has a low level of mRNA in human sperm from individuals with teratozoospermia. ( A , B ) Immunofluorescence staining displayed the localization and distribution of Fam170a protein in mouse and human seminiferous tubules, respectively. Roman numerals indicate the spermatogenic stages of the tubules in WT testicular sections. S: spermatid. The specific stages of mouse tubules were determined according to published criteria [ , ]. ( C ) Schematic summary of Fam170a protein localization during spermiogenesis. ( D ) Three different microarrays captured Fam170a gene transcription levels in sperm samples from normal and teratozoospermia men. The original data source is marked at the top of the histogram. GDS2695 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2695±FAM170A ), GDS2696 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2696+FAM170A ), and <t>GDS2697</t> ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2697+FAM170A ); ** P < .01, * P < .005. The data are displayed as mean ± SEM. A t -test was performed, and false discovery rate (FDR) P -values were shown. ( E ) The relative Fam170a mRNA abundance in sperm samples from normal ( n = 4) and teratozoospermia ( n = 3) men after qRT-PCR analysis. The control data are displayed as mean ± SEM.
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    Fam170a is located in the nucleus of spermatids at steps 9–13 and has a low level of mRNA in human sperm from individuals with teratozoospermia. ( A , B ) Immunofluorescence staining displayed the localization and distribution of Fam170a protein in mouse and human seminiferous tubules, respectively. Roman numerals indicate the spermatogenic stages of the tubules in WT testicular sections. S: spermatid. The specific stages of mouse tubules were determined according to published criteria [ , ]. ( C ) Schematic summary of Fam170a protein localization during spermiogenesis. ( D ) Three different microarrays captured Fam170a gene transcription levels in sperm samples from normal and teratozoospermia men. The original data source is marked at the top of the histogram. GDS2695 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2695±FAM170A ), GDS2696 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2696+FAM170A ), and <t>GDS2697</t> ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2697+FAM170A ); ** P < .01, * P < .005. The data are displayed as mean ± SEM. A t -test was performed, and false discovery rate (FDR) P -values were shown. ( E ) The relative Fam170a mRNA abundance in sperm samples from normal ( n = 4) and teratozoospermia ( n = 3) men after qRT-PCR analysis. The control data are displayed as mean ± SEM.
    U133 Plus2.0 Microarray, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/u133+microarray/pm39804049-74-21-21
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    Fam170a is located in the nucleus of spermatids at steps 9–13 and has a low level of mRNA in human sperm from individuals with teratozoospermia. ( A , B ) Immunofluorescence staining displayed the localization and distribution of Fam170a protein in mouse and human seminiferous tubules, respectively. Roman numerals indicate the spermatogenic stages of the tubules in WT testicular sections. S: spermatid. The specific stages of mouse tubules were determined according to published criteria [ , ]. ( C ) Schematic summary of Fam170a protein localization during spermiogenesis. ( D ) Three different microarrays captured Fam170a gene transcription levels in sperm samples from normal and teratozoospermia men. The original data source is marked at the top of the histogram. GDS2695 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2695±FAM170A ), GDS2696 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2696+FAM170A ), and <t>GDS2697</t> ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2697+FAM170A ); ** P < .01, * P < .005. The data are displayed as mean ± SEM. A t -test was performed, and false discovery rate (FDR) P -values were shown. ( E ) The relative Fam170a mRNA abundance in sperm samples from normal ( n = 4) and teratozoospermia ( n = 3) men after qRT-PCR analysis. The control data are displayed as mean ± SEM.
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    Fam170a is located in the nucleus of spermatids at steps 9–13 and has a low level of mRNA in human sperm from individuals with teratozoospermia. ( A , B ) Immunofluorescence staining displayed the localization and distribution of Fam170a protein in mouse and human seminiferous tubules, respectively. Roman numerals indicate the spermatogenic stages of the tubules in WT testicular sections. S: spermatid. The specific stages of mouse tubules were determined according to published criteria [ , ]. ( C ) Schematic summary of Fam170a protein localization during spermiogenesis. ( D ) Three different microarrays captured Fam170a gene transcription levels in sperm samples from normal and teratozoospermia men. The original data source is marked at the top of the histogram. GDS2695 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2695±FAM170A ), GDS2696 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2696+FAM170A ), and <t>GDS2697</t> ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2697+FAM170A ); ** P < .01, * P < .005. The data are displayed as mean ± SEM. A t -test was performed, and false discovery rate (FDR) P -values were shown. ( E ) The relative Fam170a mRNA abundance in sperm samples from normal ( n = 4) and teratozoospermia ( n = 3) men after qRT-PCR analysis. The control data are displayed as mean ± SEM.
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    Image Search Results


    Fam170a is located in the nucleus of spermatids at steps 9–13 and has a low level of mRNA in human sperm from individuals with teratozoospermia. ( A , B ) Immunofluorescence staining displayed the localization and distribution of Fam170a protein in mouse and human seminiferous tubules, respectively. Roman numerals indicate the spermatogenic stages of the tubules in WT testicular sections. S: spermatid. The specific stages of mouse tubules were determined according to published criteria [ , ]. ( C ) Schematic summary of Fam170a protein localization during spermiogenesis. ( D ) Three different microarrays captured Fam170a gene transcription levels in sperm samples from normal and teratozoospermia men. The original data source is marked at the top of the histogram. GDS2695 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2695±FAM170A ), GDS2696 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2696+FAM170A ), and GDS2697 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2697+FAM170A ); ** P < .01, * P < .005. The data are displayed as mean ± SEM. A t -test was performed, and false discovery rate (FDR) P -values were shown. ( E ) The relative Fam170a mRNA abundance in sperm samples from normal ( n = 4) and teratozoospermia ( n = 3) men after qRT-PCR analysis. The control data are displayed as mean ± SEM.

    Journal: Nucleic Acids Research

    Article Title: Fam170a deficiency causes male infertility by impairing histone-to-protamine exchange during mouse spermiogenesis

    doi: 10.1093/nar/gkaf023

    Figure Lengend Snippet: Fam170a is located in the nucleus of spermatids at steps 9–13 and has a low level of mRNA in human sperm from individuals with teratozoospermia. ( A , B ) Immunofluorescence staining displayed the localization and distribution of Fam170a protein in mouse and human seminiferous tubules, respectively. Roman numerals indicate the spermatogenic stages of the tubules in WT testicular sections. S: spermatid. The specific stages of mouse tubules were determined according to published criteria [ , ]. ( C ) Schematic summary of Fam170a protein localization during spermiogenesis. ( D ) Three different microarrays captured Fam170a gene transcription levels in sperm samples from normal and teratozoospermia men. The original data source is marked at the top of the histogram. GDS2695 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2695±FAM170A ), GDS2696 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2696+FAM170A ), and GDS2697 ( https://www.ncbi.nlm.nih.gov/geoprofiles/?term=GDS2697+FAM170A ); ** P < .01, * P < .005. The data are displayed as mean ± SEM. A t -test was performed, and false discovery rate (FDR) P -values were shown. ( E ) The relative Fam170a mRNA abundance in sperm samples from normal ( n = 4) and teratozoospermia ( n = 3) men after qRT-PCR analysis. The control data are displayed as mean ± SEM.

    Article Snippet: Similarly, the data from the Illumina Sentrix Expression (HumanRef-8) microbead microarray (five normal and eight teratozoospermia samples, GDS2696) and Affymetrix U133 plus2 microarray (13 normal and 8 teratozoospermia samples, GDS2697) revealed significantly decreased Fam170a mRNA abundance in the teratozoospermia samples (Fig. ; P < .05, P < .01).

    Techniques: Immunofluorescence, Staining, Quantitative RT-PCR, Control