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m mlv reverse transcriptase  (TaKaRa)


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    Structured Review

    TaKaRa m mlv reverse transcriptase
    M Mlv Reverse Transcriptase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 1786 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transcriptase/MMLV+Reverse+Transcriptase%2C+GPR/pm42869319-91-12-15
    Average 97 stars, based on 1786 article reviews
    m mlv reverse transcriptase - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    cDNA Synthesis:

    Article Title: Targeting the HIF-1α-BSG metabolic circuit reverses enzalutamide resistance in prostate cancer.
    Article Snippet: Background: Enzalutamide resistance limits durable control of castration-resistant prostate cancer (CRPC), but the mechanisms coordinating metabolic adaptation, cellular plasticity, and residual androgen receptor (AR) signaling remain incompletely understood.. We investigated whether BSG, encoding basigin/CD147, contributes to this resistant state.. Methods: We integrated reanalysis of an androgen-responsive reporter-based CRISPR activation screen with bulk and single-cell transcriptomic datasets, clinical specimens, and outcome data.

    Isolation:

    Article Title: Targeting the HIF-1α-BSG metabolic circuit reverses enzalutamide resistance in prostate cancer.
    Article Snippet: Background: Enzalutamide resistance limits durable control of castration-resistant prostate cancer (CRPC), but the mechanisms coordinating metabolic adaptation, cellular plasticity, and residual androgen receptor (AR) signaling remain incompletely understood.. We investigated whether BSG, encoding basigin/CD147, contributes to this resistant state.. Methods: We integrated reanalysis of an androgen-responsive reporter-based CRISPR activation screen with bulk and single-cell transcriptomic datasets, clinical specimens, and outcome data.

    Article Title: The circadian clock protein CmLHY integrates strigolactone signaling to regulate short-day-induced flowering in chrysanthemum.
    Article Snippet: Flowering is a key ornamental trait in chrysanthemum and a major agronomic trait 2 affecting rice yield.. Strigolactones (SLs) are a class of phytohormones that regulate 3 diverse aspects of plant growth and development, including flowering time.. However, 4 the molecular mechanisms underlying SL-mediated regulation of flowering remain 5 poorly understood.

    Reverse Transcription:

    Article Title: Targeting the HIF-1α-BSG metabolic circuit reverses enzalutamide resistance in prostate cancer.
    Article Snippet: Background: Enzalutamide resistance limits durable control of castration-resistant prostate cancer (CRPC), but the mechanisms coordinating metabolic adaptation, cellular plasticity, and residual androgen receptor (AR) signaling remain incompletely understood.. We investigated whether BSG, encoding basigin/CD147, contributes to this resistant state.. Methods: We integrated reanalysis of an androgen-responsive reporter-based CRISPR activation screen with bulk and single-cell transcriptomic datasets, clinical specimens, and outcome data.

    Article Title: A novel plant defense mechanism: NbSIPK antagonizes TYLCV coat protein-mediated viral infection
    Article Snippet: All primers were synthesized by SunGene Biotech (Shanghai) Co., Ltd. Total RNA was extracted from each sample using Trizol reagent, followed by measurement of RNA purity and concentration. .. Reverse transcription was performed using M-MLV Reverse Transcriptase (TaKaRa, Dalian, China). ..

    Article Title: Grass carp (Ctenopharyngodon idella) SIRT7 negatively regulates HIF-1 signaling pathway by interacting with HIF-1α independent of deacetylase activity under hypoxia
    Article Snippet: Hypoxia is a major environmental stressor in intensive aquaculture, threatening the survival of economically important fish like grass carp (Ctenopharyngodon idella).. Although the Hypoxia-inducible factor 1 (HIF-1) pathway plays a central role in oxygen adaptation, its regulatory mechanisms in teleosts remain unclear.. Here, we cloned the grass carp sirtuin 7 gene (Cisirt7), which encodes a conserved nuclear protein containing a classic SIR2 catalytic domain of deacetylase.

    Article Title: The circadian clock protein CmLHY integrates strigolactone signaling to regulate short-day-induced flowering in chrysanthemum.
    Article Snippet: Flowering is a key ornamental trait in chrysanthemum and a major agronomic trait 2 affecting rice yield.. Strigolactones (SLs) are a class of phytohormones that regulate 3 diverse aspects of plant growth and development, including flowering time.. However, 4 the molecular mechanisms underlying SL-mediated regulation of flowering remain 5 poorly understood.

    Article Title: Complete genome sequence of a novel isolate of Victorivirus nijyusani infecting Botrytis cinerea strain G20-5 associated with strawberry gray mold.
    Article Snippet: 1 State Key Laboratory of Agricultural and Forestry Biosecurity, MOA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Haidian District, Beijing 100193, People’s Republic of China 2 College of Grassland Science and Technology, China Agricultural University, Haidian District, Beijing 100193, People’s Republic of China Abstract In the study, a novel isolate of Victorivirus nijyusani was isolated from Botrytis cinerea strain G20-5, a causal agent of strawberry gray mold, and tentatively named Botrytis cinerea victorivirus 4 (BcVV4).. BcVV4 has a double-stranded RNA (dsRNA) of 5135 bp long and contains two open reading frames (ORFs) 1 and 2, which overlap in the AUGA sequence.. They are predicted to encode a coat protein (CP) of 756-amino acid (aa) with a molecular weight of 80.2 kDa, and an RNA-dependent RNA polymerase (RdRp) of 850-aa with a molecular weight of 93.3 kDa, respectively.

    Article Title: Fibroblast Growth Factor 7 Limits Fibroblast-Driven Matrix Remodeling in Abdominal Aortic Aneurysm.
    Article Snippet: 1 Department of Vascular Surgery, Taizhou University Affiliated Municipal Hospital, Taizhou, Zhejiang 318000, China 2 Department of Vascular Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiaotong University school of medicine, Shanghai 200011, China Abstract Abdominal aortic aneurysm (AAA) is a chronic vascular disorder characterized by progressive aortic dilation and extracellular matrix remodeling, for which effective pharmacological therapies remain limited.. Fibroblast growth factor 7 (FGF7), a paracrine growth factor involved in tissue repair and remodeling, has not been fully investigated in AAA.. Transcriptomic analyses of GSE239620 and GSE57691 revealed dysregulated FGF7 expression in aneurysmal tissues, with predominant localization in fibroblasts.

    Article Title: Antagonistic regulation by mango MiSPL9a and MiSPL9b regulates flowering time, drought and salt stress in Arabidopsis.
    Article Snippet: SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factors, which are unique to plants, contain a highly conserved SBP domain that regulates gene expression by binding to downstream targets.. They play critical roles in various biological processes, especially in the regulation of flowering in plants.. In this study, two SPL-like genes (MiSPL9a and MiSPL9b) were identified from mango genomic and transcriptomic data, and their sequence, expression and function were further analyzed.

    Synthesized:

    Article Title: Grass carp (Ctenopharyngodon idella) SIRT7 negatively regulates HIF-1 signaling pathway by interacting with HIF-1α independent of deacetylase activity under hypoxia
    Article Snippet: Hypoxia is a major environmental stressor in intensive aquaculture, threatening the survival of economically important fish like grass carp (Ctenopharyngodon idella).. Although the Hypoxia-inducible factor 1 (HIF-1) pathway plays a central role in oxygen adaptation, its regulatory mechanisms in teleosts remain unclear.. Here, we cloned the grass carp sirtuin 7 gene (Cisirt7), which encodes a conserved nuclear protein containing a classic SIR2 catalytic domain of deacetylase.

    Article Title: The circadian clock protein CmLHY integrates strigolactone signaling to regulate short-day-induced flowering in chrysanthemum.
    Article Snippet: Flowering is a key ornamental trait in chrysanthemum and a major agronomic trait 2 affecting rice yield.. Strigolactones (SLs) are a class of phytohormones that regulate 3 diverse aspects of plant growth and development, including flowering time.. However, 4 the molecular mechanisms underlying SL-mediated regulation of flowering remain 5 poorly understood.

    Article Title: Fibroblast Growth Factor 7 Limits Fibroblast-Driven Matrix Remodeling in Abdominal Aortic Aneurysm.
    Article Snippet: 1 Department of Vascular Surgery, Taizhou University Affiliated Municipal Hospital, Taizhou, Zhejiang 318000, China 2 Department of Vascular Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiaotong University school of medicine, Shanghai 200011, China Abstract Abdominal aortic aneurysm (AAA) is a chronic vascular disorder characterized by progressive aortic dilation and extracellular matrix remodeling, for which effective pharmacological therapies remain limited.. Fibroblast growth factor 7 (FGF7), a paracrine growth factor involved in tissue repair and remodeling, has not been fully investigated in AAA.. Transcriptomic analyses of GSE239620 and GSE57691 revealed dysregulated FGF7 expression in aneurysmal tissues, with predominant localization in fibroblasts.

    Article Title: Antagonistic regulation by mango MiSPL9a and MiSPL9b regulates flowering time, drought and salt stress in Arabidopsis.
    Article Snippet: SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factors, which are unique to plants, contain a highly conserved SBP domain that regulates gene expression by binding to downstream targets.. They play critical roles in various biological processes, especially in the regulation of flowering in plants.. In this study, two SPL-like genes (MiSPL9a and MiSPL9b) were identified from mango genomic and transcriptomic data, and their sequence, expression and function were further analyzed.

    Generated:

    Article Title: Complete genome sequence of a novel isolate of Victorivirus nijyusani infecting Botrytis cinerea strain G20-5 associated with strawberry gray mold.
    Article Snippet: 1 State Key Laboratory of Agricultural and Forestry Biosecurity, MOA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Haidian District, Beijing 100193, People’s Republic of China 2 College of Grassland Science and Technology, China Agricultural University, Haidian District, Beijing 100193, People’s Republic of China Abstract In the study, a novel isolate of Victorivirus nijyusani was isolated from Botrytis cinerea strain G20-5, a causal agent of strawberry gray mold, and tentatively named Botrytis cinerea victorivirus 4 (BcVV4).. BcVV4 has a double-stranded RNA (dsRNA) of 5135 bp long and contains two open reading frames (ORFs) 1 and 2, which overlap in the AUGA sequence.. They are predicted to encode a coat protein (CP) of 756-amino acid (aa) with a molecular weight of 80.2 kDa, and an RNA-dependent RNA polymerase (RdRp) of 850-aa with a molecular weight of 93.3 kDa, respectively.

    Article Title: Acetylation-driven non-canonical transcriptional repressor ITSN1-S targets RASGRF1-GPX4 axis to induce breast cancer ferroptosis and anti-tumor immune remodeling
    Article Snippet: Total RNA was isolated from cells using Trizol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions. .. cDNA was generated by the RTase M-MLV (Takara, Shiga-ken, Japan) as described in the Cell Death and Disease manufacturer’s protocol. .. Quantification of target mRNA level was done by RT-qPCR using SYBR Green PCR Master Mix (TaKaRa, Shiga-ken, Japan), and the expression of GAPDH was used as the internal control.

    Polymerase Chain Reaction:

    Article Title: Complete genome sequence of a novel isolate of Victorivirus nijyusani infecting Botrytis cinerea strain G20-5 associated with strawberry gray mold.
    Article Snippet: 1 State Key Laboratory of Agricultural and Forestry Biosecurity, MOA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Haidian District, Beijing 100193, People’s Republic of China 2 College of Grassland Science and Technology, China Agricultural University, Haidian District, Beijing 100193, People’s Republic of China Abstract In the study, a novel isolate of Victorivirus nijyusani was isolated from Botrytis cinerea strain G20-5, a causal agent of strawberry gray mold, and tentatively named Botrytis cinerea victorivirus 4 (BcVV4).. BcVV4 has a double-stranded RNA (dsRNA) of 5135 bp long and contains two open reading frames (ORFs) 1 and 2, which overlap in the AUGA sequence.. They are predicted to encode a coat protein (CP) of 756-amino acid (aa) with a molecular weight of 80.2 kDa, and an RNA-dependent RNA polymerase (RdRp) of 850-aa with a molecular weight of 93.3 kDa, respectively.



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