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smartscribe reverse transcriptase  (TaKaRa)


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    Structured Review

    TaKaRa smartscribe reverse transcriptase
    Smartscribe Reverse Transcriptase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1130 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transcriptase/SMARTScribe+Reverse+Transcriptase/pmc13076092-32-22-25
    Average 96 stars, based on 1130 article reviews
    smartscribe reverse transcriptase - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Synthesized:

    Article Title: DC-STAMP and OC-STAMP cooperatively regulate osteoclast and foreign body giant cell cell-cell fusion.
    Article Snippet: Total RNA was isolated from osteoclasts or FBGCs using an RNeasy mini kit (Qiagen, Hilden, Germany), as described above. .. Single-stranded complementary DNAs (cDNAs) were synthesized with reverse transcriptase (Takara Bio Inc, Shiga Japan). .. Real-time PCR was performed using TB Green®Premix Ex Taq GC (Takara Bio Inc, Shiga Japan) with a DICE Thermal cycler (Takara Bio Inc.), according to the manufacturer’s instructions.

    Article Title: Depletion of tropomyosin 1.6 alleviates TGF-β1-induced fibroblast activation by promoting the α-SMA-MMP9 matrix-degrading structure
    Article Snippet: .. Total cell RNAs were extracted by TRIzol (Invitrogen), and complementary DNA was synthesized from 1000 ng of total RNA with oligo-dT/random primers and the reverse transcriptase (Takara). ..

    Reverse Transcription:

    Article Title: DC-STAMP and OC-STAMP cooperatively regulate osteoclast and foreign body giant cell cell-cell fusion.
    Article Snippet: Total RNA was isolated from osteoclasts or FBGCs using an RNeasy mini kit (Qiagen, Hilden, Germany), as described above. .. Single-stranded complementary DNAs (cDNAs) were synthesized with reverse transcriptase (Takara Bio Inc, Shiga Japan). .. Real-time PCR was performed using TB Green®Premix Ex Taq GC (Takara Bio Inc, Shiga Japan) with a DICE Thermal cycler (Takara Bio Inc.), according to the manufacturer’s instructions.

    Article Title: Depletion of tropomyosin 1.6 alleviates TGF-β1-induced fibroblast activation by promoting the α-SMA-MMP9 matrix-degrading structure
    Article Snippet: .. Total cell RNAs were extracted by TRIzol (Invitrogen), and complementary DNA was synthesized from 1000 ng of total RNA with oligo-dT/random primers and the reverse transcriptase (Takara). ..

    Article Title: DGKβ accelerates the progression of cervical cancer through ANGPT4-mediated tumor angiogenesis.
    Article Snippet: Background: Cervical cancer (CC) is a major cause of morbidity and mortality in women, with complex etiology and progression.. Diacylglycerol kinases (DGKs) are pivotal in lipid metabolism.. Although diacylglycerol kinase beta (DGKβ) is well-studied in neurology, its role in cancer, especially CC, remains underexplored.

    Article Title: WATER NEWS: a field approach for sustainable detection of pathogens in wastewater
    Article Snippet: .. To evaluate the LoD of RNA and assay performance on real samples, 0.8 μL of reverse transcriptase (Shanghai Takara Bio Co., China) was added to the one-pot reaction mixture, followed by fluorescence measurements at 37°C or 42°C. ..

    Article Title: Rab10 plays a protective role in the development of pathological cardiac hypertrophy
    Article Snippet: Total RNA was extracted from cultured cardiomyocytes or fresh mouse hearts using the TRIzol reagent (Invitrogen). .. RNA (1–2 μg) was reverse transcribed to generate first-strand cDNA with reverse transcriptase (Takara, Japan). .. Quantitative real-time PCR (qPCR) analysis was run in triplicate using the ABI 7500 detector system according to manufacturer's instructions.

    Article Title: circRNA-associated-ceRNA networks in patients with traumatic tracheal stenosis.
    Article Snippet: Verification of hub circRNAs, miRNAs, and mRNA by qRT-PCR Through integrated bioinformatics analysis(Mireap, MIRanda, TargetScan, miRTarBase and circBase), we established a competing endogenous RNA network. .. Given that inflammation and fibrosis represent the predominant histopathological manifestations in TTS, we prioritized the hsa_circ_030284/hsa-miR-12075p/S100A2 axis for experimental validation via qRT-PCR.Through employing the reverse transcriptase (Takara, China) in accordance with the protocol of the manufacturer, the total RNAs of 6 of TTS tissues and 6 of normal control tissues extracted by utilizing the TRIzol reagent (Takara, China) were then reversely transcribed. .. The qRT-PCR was then performed by applying the QuantStudioTM 5 Real-Time PCR System (Agilent Technologies) by utilizing the SYBR Green PCR Kit (Takara) in accordance with the manufacturer’s protocol.

    Article Title: Revealing the expression pattern of FLO gene family in Saccharomyces pastorianus
    Article Snippet: In industrial beer production, flocculation of yeast is an environmentally friendly and cost-effective way to separate yeast cells from fermentation broth for subsequent production.. Revealing the mechanism of flocculation will help breweries improve production technology for better regulation of yeast flocculation.. The FLO gene family are well-known for mediating flocculation ability.

    Article Title: circRNA-associated-ceRNA networks in patients with traumatic tracheal stenosis
    Article Snippet: Through integrated bioinformatics analysis(Mireap, MIRanda, TargetScan, miRTarBase and circBase), we established a competing endogenous RNA network. .. Given that inflammation and fibrosis represent the predominant histopathological manifestations in TTS, we prioritized the hsa_circ_030284/hsa-miR-1207-5p/ S100A2 axis for experimental validation via qRT-PCR.Through employing the reverse transcriptase (Takara, China) in accordance with the protocol of the manufacturer, the total RNAs of 6 of TTS tissues and 6 of normal control tissues extracted by utilizing the TRIzol reagent (Takara, China) were then reversely transcribed. .. The qRT-PCR was then performed by applying the QuantStudioTM 5 Real-Time PCR System (Agilent Technologies) by utilizing the SYBR Green PCR Kit (Takara) in accordance with the manufacturer’s protocol.

    Isolation:

    Article Title: DGKβ accelerates the progression of cervical cancer through ANGPT4-mediated tumor angiogenesis.
    Article Snippet: Background: Cervical cancer (CC) is a major cause of morbidity and mortality in women, with complex etiology and progression.. Diacylglycerol kinases (DGKs) are pivotal in lipid metabolism.. Although diacylglycerol kinase beta (DGKβ) is well-studied in neurology, its role in cancer, especially CC, remains underexplored.

    Fluorescence:

    Article Title: WATER NEWS: a field approach for sustainable detection of pathogens in wastewater
    Article Snippet: .. To evaluate the LoD of RNA and assay performance on real samples, 0.8 μL of reverse transcriptase (Shanghai Takara Bio Co., China) was added to the one-pot reaction mixture, followed by fluorescence measurements at 37°C or 42°C. ..

    Biomarker Discovery:

    Article Title: circRNA-associated-ceRNA networks in patients with traumatic tracheal stenosis.
    Article Snippet: Verification of hub circRNAs, miRNAs, and mRNA by qRT-PCR Through integrated bioinformatics analysis(Mireap, MIRanda, TargetScan, miRTarBase and circBase), we established a competing endogenous RNA network. .. Given that inflammation and fibrosis represent the predominant histopathological manifestations in TTS, we prioritized the hsa_circ_030284/hsa-miR-12075p/S100A2 axis for experimental validation via qRT-PCR.Through employing the reverse transcriptase (Takara, China) in accordance with the protocol of the manufacturer, the total RNAs of 6 of TTS tissues and 6 of normal control tissues extracted by utilizing the TRIzol reagent (Takara, China) were then reversely transcribed. .. The qRT-PCR was then performed by applying the QuantStudioTM 5 Real-Time PCR System (Agilent Technologies) by utilizing the SYBR Green PCR Kit (Takara) in accordance with the manufacturer’s protocol.

    Article Title: circRNA-associated-ceRNA networks in patients with traumatic tracheal stenosis
    Article Snippet: Through integrated bioinformatics analysis(Mireap, MIRanda, TargetScan, miRTarBase and circBase), we established a competing endogenous RNA network. .. Given that inflammation and fibrosis represent the predominant histopathological manifestations in TTS, we prioritized the hsa_circ_030284/hsa-miR-1207-5p/ S100A2 axis for experimental validation via qRT-PCR.Through employing the reverse transcriptase (Takara, China) in accordance with the protocol of the manufacturer, the total RNAs of 6 of TTS tissues and 6 of normal control tissues extracted by utilizing the TRIzol reagent (Takara, China) were then reversely transcribed. .. The qRT-PCR was then performed by applying the QuantStudioTM 5 Real-Time PCR System (Agilent Technologies) by utilizing the SYBR Green PCR Kit (Takara) in accordance with the manufacturer’s protocol.

    Control:

    Article Title: circRNA-associated-ceRNA networks in patients with traumatic tracheal stenosis.
    Article Snippet: Verification of hub circRNAs, miRNAs, and mRNA by qRT-PCR Through integrated bioinformatics analysis(Mireap, MIRanda, TargetScan, miRTarBase and circBase), we established a competing endogenous RNA network. .. Given that inflammation and fibrosis represent the predominant histopathological manifestations in TTS, we prioritized the hsa_circ_030284/hsa-miR-12075p/S100A2 axis for experimental validation via qRT-PCR.Through employing the reverse transcriptase (Takara, China) in accordance with the protocol of the manufacturer, the total RNAs of 6 of TTS tissues and 6 of normal control tissues extracted by utilizing the TRIzol reagent (Takara, China) were then reversely transcribed. .. The qRT-PCR was then performed by applying the QuantStudioTM 5 Real-Time PCR System (Agilent Technologies) by utilizing the SYBR Green PCR Kit (Takara) in accordance with the manufacturer’s protocol.

    Article Title: circRNA-associated-ceRNA networks in patients with traumatic tracheal stenosis
    Article Snippet: Through integrated bioinformatics analysis(Mireap, MIRanda, TargetScan, miRTarBase and circBase), we established a competing endogenous RNA network. .. Given that inflammation and fibrosis represent the predominant histopathological manifestations in TTS, we prioritized the hsa_circ_030284/hsa-miR-1207-5p/ S100A2 axis for experimental validation via qRT-PCR.Through employing the reverse transcriptase (Takara, China) in accordance with the protocol of the manufacturer, the total RNAs of 6 of TTS tissues and 6 of normal control tissues extracted by utilizing the TRIzol reagent (Takara, China) were then reversely transcribed. .. The qRT-PCR was then performed by applying the QuantStudioTM 5 Real-Time PCR System (Agilent Technologies) by utilizing the SYBR Green PCR Kit (Takara) in accordance with the manufacturer’s protocol.



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