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high- capacity cdna reverse transcription kit (cat. number 4374966  (Thermo Fisher)


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    Structured Review

    Thermo Fisher high- capacity cdna reverse transcription kit (cat. number 4374966
    High Capacity Cdna Reverse Transcription Kit (Cat. Number 4374966, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transcript+numbers/pm39474783-88-47-56
    Average 90 stars, based on 1 article reviews
    high- capacity cdna reverse transcription kit (cat. number 4374966 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Synthesized:

    Article Title: High-fat diet mouse model receiving L-glucose supplementations propagates liver injury
    Article Snippet: .. Complementary DNA (cDNA) was synthesized from 2 μg of total RNA using a High-Capacity cDNA Reverse Transcription Kit with RNase Inhibitor (Applied Biosystems, Cat # 4374966) following the manufacturer’s instructions. ..

    Reverse Transcription:

    Article Title: High-fat diet mouse model receiving L-glucose supplementations propagates liver injury
    Article Snippet: .. Complementary DNA (cDNA) was synthesized from 2 μg of total RNA using a High-Capacity cDNA Reverse Transcription Kit with RNase Inhibitor (Applied Biosystems, Cat # 4374966) following the manufacturer’s instructions. ..

    Article Title: Seeding-competent TDP-43 persists in human patient and mouse muscle.
    Article Snippet: RNA was extracted from tibialis anterior muscles by homogenization in Tri Reagent (Molecular Research Center Inc.) according to the reagent protocol. .. Total RNA was converted to cDNA using the High Capacity cDNA Reverse Transcription Kit (Applied Biosciences, 4374966). .. Primers for Sh3bgr and Tns1 cryptic exon detection (table S8) were used to amplify the targets using the DreamTaq 2× master mix (Thermo Fisher Scientific, K1081).

    other:

    Article Title: CRISPR screens unveil nutrient-dependent lysosomal and mitochondrial nodes impacting intestinal tissue-resident memory CD8 + T cell formation
    Article Snippet: Briefly, all RNA was isolated from 1×10 5 sort-purified splenic or siIEL OT-I cells using the RNeasy Micro Kit according to the manufacturer’s instructions (catalog #74004, Qiagen), and mRNA was reverse transcribed to cDNA for subsequent real-time PCR analysis using High-Capacity cDNA Reverse Transcription kit (catalog #4374966, Thermo Fisher Scientific).

    Article Title: CRISPR screens unveil nutrient-dependent lysosomal and mitochondrial nodes impacting intestinal tissue-resident memory CD8 + T cell formation.
    Article Snippet: Gene amplification was assessed using an Applied Biosystems QuantStudio 7 Flex quantitative PCR machine (Thermo Fisher Scientific).

    Article Title: Lactate reprograms glioblastoma immunity through CBX3-regulated histone lactylation
    Article Snippet: RNA was extracted using the Direct-zol RNA Microprep Kit (Zymo Research, R2062), according to the manufacturer’s protocol. cDNA was synthesized with 1 μg RNA by reverse transcription using the High-Capacity cDNA Reverse Transcription Kit (Life Technologies, 4374966).

    Article Title: HIF1α gates tendon response to overload and drives tendinopathy independently of vascular recruitment
    Article Snippet: Isolated RNA was stored at -80°C or processed immediately for cDNA synthesis using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, 4374966) as described in the manufacturer’s instructions.

    Article Title: Ramp sequence may explain synonymous variant association with Alzheimer’s disease in the Paired Immunoglobulin-like Type 2 Receptor Alpha ( PILRA )
    Article Snippet: Reverse transcription of the RNA into complementary DNA (cDNA) was then performed to convert the RNA molecules into their corresponding cDNA sequences using the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific, Catalog #4374966).

    Article Title: Epigenetic Regulation of HOXA3 and its Impact on Oral Squamous Cell Carcinoma Progression
    Article Snippet: One microgram of isolated total RNA was converted into cDNA using a High-capacity cDNA reverse transcription kit (Cat. No. 4374966, Applied Biosystems) following the manufacturer’s instructions.



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    Image Search Results


    Correlations between AMP expression and mucosal small intestinal microbiota revealed a novel microbe capable of inducing Reg3g expression.

    Journal: Gut Microbes

    Article Title: Disentangling the impact of obesity, diet, host factors, and microbiota on small intestinal antimicrobial peptide expression

    doi: 10.1080/19490976.2025.2536095

    Figure Lengend Snippet: Correlations between AMP expression and mucosal small intestinal microbiota revealed a novel microbe capable of inducing Reg3g expression.

    Article Snippet: Different factors can impact AMP expression in the murine small intestine. (a, b, c) Schematic experimental setup of the dietary intervention experiments: (a) Intervention 1, male Charles River mice fed a chow or a high-fat diet (HFD) for 8 weeks; (b) Intervention 2, male and female Charles River mice fed a chow or a Western-style diet (WSD) for 8 weeks; (c) Intervention 3, male and female Taconic mice fed a chow or a WSD for 8 weeks. (d) Total amounts of all measured AMP transcripts in the ileum of all mice fed a chow, WSD, or HFD. (e) Total amounts of all measured AMP transcripts in the ileum of male and female mice fed a chow or WSD from interventions 2 and 3. (f) Reg3g transcript copy number in the ileum of chow and WSD fed mice from interventions 2 and 3. (g) Total amounts of all measured AMP transcripts in the ileum of male Charles River or Taconic mice fed a chow or a WSD. (h) Reg3g transcript copy number in the ileum of male Charles River or Taconic mice fed a chow or a WSD. (i) Total amounts of all measured AMP transcripts in the ileum of female Charles River or Taconic mice fed a chow or a WSD. (j) Reg3g transcript copy number in the ileum of female Charles River or Taconic mice fed a chow or a WSD. (k) Principal component analysis (PCoA) evaluating the effect of diet, mouse vendor, sex, or intervention on AMP expression in mice from the dietary interventions.

    Techniques: Expressing

    Patient demographics and tumor characteristics and association of  ALKBH5  levels with clinicopathological features.

    Journal: Scientific Reports

    Article Title: ALKBH5, an m6A demethylase, attenuates tumor growth and inhibits metastasis in papillary thyroid carcinoma

    doi: 10.1038/s41598-024-84352-w

    Figure Lengend Snippet: Patient demographics and tumor characteristics and association of ALKBH5 levels with clinicopathological features.

    Article Snippet: The CDS sequence of the ALKBH5 gene (Transcript accession number: NM_017758.4) was retrieved from the National Center for Biotechnology Information.

    Techniques: Expressing

    ( A ) qRT-PCR and Western blot were used to detect the expression of ALKBH5 to screen PTC cells. ( * P < 0.05 indicates a significant difference between each group compared to the Nthy-ori3-1 group). ( B ) Subcellular Localization of ALKBH5 Protein Detected by Immunofluorescence.

    Journal: Scientific Reports

    Article Title: ALKBH5, an m6A demethylase, attenuates tumor growth and inhibits metastasis in papillary thyroid carcinoma

    doi: 10.1038/s41598-024-84352-w

    Figure Lengend Snippet: ( A ) qRT-PCR and Western blot were used to detect the expression of ALKBH5 to screen PTC cells. ( * P < 0.05 indicates a significant difference between each group compared to the Nthy-ori3-1 group). ( B ) Subcellular Localization of ALKBH5 Protein Detected by Immunofluorescence.

    Article Snippet: The CDS sequence of the ALKBH5 gene (Transcript accession number: NM_017758.4) was retrieved from the National Center for Biotechnology Information.

    Techniques: Quantitative RT-PCR, Western Blot, Expressing, Immunofluorescence

    Transfection validation of ALKBH5 overexpression and interference ( A . IHH4 cells; B . TPC-1 cells).

    Journal: Scientific Reports

    Article Title: ALKBH5, an m6A demethylase, attenuates tumor growth and inhibits metastasis in papillary thyroid carcinoma

    doi: 10.1038/s41598-024-84352-w

    Figure Lengend Snippet: Transfection validation of ALKBH5 overexpression and interference ( A . IHH4 cells; B . TPC-1 cells).

    Article Snippet: The CDS sequence of the ALKBH5 gene (Transcript accession number: NM_017758.4) was retrieved from the National Center for Biotechnology Information.

    Techniques: Transfection, Biomarker Discovery, Over Expression

    Proliferation and invasion of IHH4 and TPC-1 Cells Detected by CCK-8 Assay and Transwell Assay ( A . CCK-8 assay to detect the cell viability of IHH4 and TPC-1 cells; B . Transwell assay to assess the invasion of IHH4 cells; C . Transwell assay to assess the invasion of TPC-1 cells. * P < 0.05 indicates a significant difference between each group compared to the OE-NC group; # P < 0.05 indicates a significant difference between each group compared to the OE-ALKBH5 group; & P < 0.05 indicates a significant difference between each group compared to the Si-NC group).

    Journal: Scientific Reports

    Article Title: ALKBH5, an m6A demethylase, attenuates tumor growth and inhibits metastasis in papillary thyroid carcinoma

    doi: 10.1038/s41598-024-84352-w

    Figure Lengend Snippet: Proliferation and invasion of IHH4 and TPC-1 Cells Detected by CCK-8 Assay and Transwell Assay ( A . CCK-8 assay to detect the cell viability of IHH4 and TPC-1 cells; B . Transwell assay to assess the invasion of IHH4 cells; C . Transwell assay to assess the invasion of TPC-1 cells. * P < 0.05 indicates a significant difference between each group compared to the OE-NC group; # P < 0.05 indicates a significant difference between each group compared to the OE-ALKBH5 group; & P < 0.05 indicates a significant difference between each group compared to the Si-NC group).

    Article Snippet: The CDS sequence of the ALKBH5 gene (Transcript accession number: NM_017758.4) was retrieved from the National Center for Biotechnology Information.

    Techniques: CCK-8 Assay, Transwell Assay

    m6A Methylation detection (A for IHH4 Cells; B for TPC-1 Cells. * P < 0.05 indicates a significant difference between each group compared to the OE-NC group; # P < 0.05 indicates a significant difference between each group compared to the OE-ALKBH5 group; & P < 0.05 indicates a significant difference between each group compared to the Si-NC group).

    Journal: Scientific Reports

    Article Title: ALKBH5, an m6A demethylase, attenuates tumor growth and inhibits metastasis in papillary thyroid carcinoma

    doi: 10.1038/s41598-024-84352-w

    Figure Lengend Snippet: m6A Methylation detection (A for IHH4 Cells; B for TPC-1 Cells. * P < 0.05 indicates a significant difference between each group compared to the OE-NC group; # P < 0.05 indicates a significant difference between each group compared to the OE-ALKBH5 group; & P < 0.05 indicates a significant difference between each group compared to the Si-NC group).

    Article Snippet: The CDS sequence of the ALKBH5 gene (Transcript accession number: NM_017758.4) was retrieved from the National Center for Biotechnology Information.

    Techniques: Methylation