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superscript tm iii reverse transcriptase system  (Thermo Fisher)


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    Thermo Fisher superscript tm iii reverse transcriptase system
    Superscript Tm Iii Reverse Transcriptase System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+tm+iii+reverse+transcriptase/superscript+iii+reverse+transcriptase/pm40609538-844-36-42
    Average 90 stars, based on 1 article reviews
    superscript tm iii reverse transcriptase system - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Quantitative RT-PCR:

    Article Title: TERRA R-loops trigger a switch in telomere maintenance towards break-induced replication and PRIMPOL-dependent repair.
    Article Snippet: .. For RT-qPCR analysis of TERRA levels, 3 μg of RNA was reverse transcribed using 200 U of SuperScript III reverse transcriptase (ThermoFisher Scientific), along with GAPDH and TERRA reverse primers (see “Reagents and Tools Table”). ..

    Reverse Transcription:

    Article Title: TERRA R-loops trigger a switch in telomere maintenance towards break-induced replication and PRIMPOL-dependent repair.
    Article Snippet: .. For RT-qPCR analysis of TERRA levels, 3 μg of RNA was reverse transcribed using 200 U of SuperScript III reverse transcriptase (ThermoFisher Scientific), along with GAPDH and TERRA reverse primers (see “Reagents and Tools Table”). ..

    Article Title: Deletion viral genome diversity among bovine viral diarrhea virus (BVDV) 1a and 1b strains.
    Article Snippet: .. At the time of plaque picking after each passage at 2 different MOIs, viral RNA was extracted, cDNA was generated via Superscript III reverse transcriptase (Thermo Fisher Scientific, Inc.), and whole-genome sequencing was conducted as previously described and detailed above [45, 52]. .. Statistical analysis tests were performed as indicated in each figure legend via GraphPad Prism version 10.2.0 (GraphPad Software, Inc.).

    Article Title: MicroRNA-196a increases apoptosis in B cells through downregulation of FOXO1
    Article Snippet: .. For target gene expression analysis, total RNA was reverse-transcribed into cDNA using SuperScript III Reverse Transcriptase (Thermo Fisher Scientific, Waltham, MA, USA) and Oligo (dT) 12 to 18 primers (Thermo Fisher Scientific) following the manufacturer’s protocol. .. The cDNA was then amplified using SYBR Green PCR Master Mix (Applied Biosystems, Foster City, CA, USA) with the specific primers.

    Article Title: Epigenetic profiling identifies markers of endocrine resistance and therapeutic options for metastatic castration-resistant prostate cancer.
    Article Snippet: .. In brief, after siRNA transfection using the abovementioned protocol RNA was isolated using RNAGEM kit (MicroGEM), and quantified by Quant-iT TM RiboGreen TM RNA Assay Kit (Thermo Fisher Scientific), following quantification cDNA was synthesized using the SuperScript TM III Reverse Transcriptase system (Thermo Fisher Scientific, USA) with random hexamer primers according to the instructions provided by manufacturers. .. Quantitative PCR (qPCR) was performed using the SensiMix TM SYBR Kit (Bioline, UK) according to the manufacturer’s instructions on a QuantStudio TM 6 Flex System (Thermo Fisher Scientific, USA).

    Article Title: Root-derived cytokinin regulates Arabidopsis flowering time through components of the age pathway.
    Article Snippet: 3 4 RNA extraction and analysis of gene expression 5 Total RNA was extracted from hand-dissected shoot apices, leaves or whole rosettes as indicated 6 at Zeitgeber 4 (4 h after the beginning of the light period) with the TRIzol method according to the 7 manufacturer (Thermo Fisher Scientific). .. For cDNA synthesis, 1 μg of total RNA was used in a 8 10 μL SuperScript III Reverse Transcriptase reaction (Thermo Fisher Scientific). ..


    other:

    Article Title: NKG2D receptor ligands are cell surface biomarkers for injured murine and human nociceptive sensory neurons
    Article Snippet: Samples were heated to 65°C for 5 min and cooled on ice before the addition of reaction buffer containing reverse-transcriptase (Superscript III, Invitrogen, Life Technologies). cDNA was synthesised on a thermo-cycler (Biorad T100) (25°C, 5 min; 50°C, 100 min; 85°C, 5 min) and finally 37°C for 20 min in the presence of RNase H. cDNA was stored at −20°C.

    Article Title: IFNα/JAK/STAT1 Axis-Induced FBXO4 Modulates Muscle Cell Differentiation via β-Catenin Degradation in Dermatomyositis
    Article Snippet: First-strand cDNA synthesis was performed using reverse transcriptase (SuperScript III, Thermo Fisher) and random hexamer primers, followed by second-strand cDNA synthesis with DNA polymerase I and RNase H. Double-stranded cDNA was purified using AMPure XP beads (Beckman Coulter), followed by A-tailing and ligation of sequencing adapters (Illumina TruSeq RNA Library Prep Kit).

    Generated:

    Article Title: Deletion viral genome diversity among bovine viral diarrhea virus (BVDV) 1a and 1b strains.
    Article Snippet: .. At the time of plaque picking after each passage at 2 different MOIs, viral RNA was extracted, cDNA was generated via Superscript III reverse transcriptase (Thermo Fisher Scientific, Inc.), and whole-genome sequencing was conducted as previously described and detailed above [45, 52]. .. Statistical analysis tests were performed as indicated in each figure legend via GraphPad Prism version 10.2.0 (GraphPad Software, Inc.).

    Sequencing:

    Article Title: Deletion viral genome diversity among bovine viral diarrhea virus (BVDV) 1a and 1b strains.
    Article Snippet: .. At the time of plaque picking after each passage at 2 different MOIs, viral RNA was extracted, cDNA was generated via Superscript III reverse transcriptase (Thermo Fisher Scientific, Inc.), and whole-genome sequencing was conducted as previously described and detailed above [45, 52]. .. Statistical analysis tests were performed as indicated in each figure legend via GraphPad Prism version 10.2.0 (GraphPad Software, Inc.).

    Targeted Gene Expression:

    Article Title: MicroRNA-196a increases apoptosis in B cells through downregulation of FOXO1
    Article Snippet: .. For target gene expression analysis, total RNA was reverse-transcribed into cDNA using SuperScript III Reverse Transcriptase (Thermo Fisher Scientific, Waltham, MA, USA) and Oligo (dT) 12 to 18 primers (Thermo Fisher Scientific) following the manufacturer’s protocol. .. The cDNA was then amplified using SYBR Green PCR Master Mix (Applied Biosystems, Foster City, CA, USA) with the specific primers.

    Transfection:

    Article Title: Epigenetic profiling identifies markers of endocrine resistance and therapeutic options for metastatic castration-resistant prostate cancer.
    Article Snippet: .. In brief, after siRNA transfection using the abovementioned protocol RNA was isolated using RNAGEM kit (MicroGEM), and quantified by Quant-iT TM RiboGreen TM RNA Assay Kit (Thermo Fisher Scientific), following quantification cDNA was synthesized using the SuperScript TM III Reverse Transcriptase system (Thermo Fisher Scientific, USA) with random hexamer primers according to the instructions provided by manufacturers. .. Quantitative PCR (qPCR) was performed using the SensiMix TM SYBR Kit (Bioline, UK) according to the manufacturer’s instructions on a QuantStudio TM 6 Flex System (Thermo Fisher Scientific, USA).

    Isolation:

    Article Title: Epigenetic profiling identifies markers of endocrine resistance and therapeutic options for metastatic castration-resistant prostate cancer.
    Article Snippet: .. In brief, after siRNA transfection using the abovementioned protocol RNA was isolated using RNAGEM kit (MicroGEM), and quantified by Quant-iT TM RiboGreen TM RNA Assay Kit (Thermo Fisher Scientific), following quantification cDNA was synthesized using the SuperScript TM III Reverse Transcriptase system (Thermo Fisher Scientific, USA) with random hexamer primers according to the instructions provided by manufacturers. .. Quantitative PCR (qPCR) was performed using the SensiMix TM SYBR Kit (Bioline, UK) according to the manufacturer’s instructions on a QuantStudio TM 6 Flex System (Thermo Fisher Scientific, USA).

    Synthesized:

    Article Title: Epigenetic profiling identifies markers of endocrine resistance and therapeutic options for metastatic castration-resistant prostate cancer.
    Article Snippet: .. In brief, after siRNA transfection using the abovementioned protocol RNA was isolated using RNAGEM kit (MicroGEM), and quantified by Quant-iT TM RiboGreen TM RNA Assay Kit (Thermo Fisher Scientific), following quantification cDNA was synthesized using the SuperScript TM III Reverse Transcriptase system (Thermo Fisher Scientific, USA) with random hexamer primers according to the instructions provided by manufacturers. .. Quantitative PCR (qPCR) was performed using the SensiMix TM SYBR Kit (Bioline, UK) according to the manufacturer’s instructions on a QuantStudio TM 6 Flex System (Thermo Fisher Scientific, USA).

    Random Hexamer:

    Article Title: Epigenetic profiling identifies markers of endocrine resistance and therapeutic options for metastatic castration-resistant prostate cancer.
    Article Snippet: .. In brief, after siRNA transfection using the abovementioned protocol RNA was isolated using RNAGEM kit (MicroGEM), and quantified by Quant-iT TM RiboGreen TM RNA Assay Kit (Thermo Fisher Scientific), following quantification cDNA was synthesized using the SuperScript TM III Reverse Transcriptase system (Thermo Fisher Scientific, USA) with random hexamer primers according to the instructions provided by manufacturers. .. Quantitative PCR (qPCR) was performed using the SensiMix TM SYBR Kit (Bioline, UK) according to the manufacturer’s instructions on a QuantStudio TM 6 Flex System (Thermo Fisher Scientific, USA).

    cDNA Synthesis:

    Article Title: Root-derived cytokinin regulates Arabidopsis flowering time through components of the age pathway.
    Article Snippet: 3 4 RNA extraction and analysis of gene expression 5 Total RNA was extracted from hand-dissected shoot apices, leaves or whole rosettes as indicated 6 at Zeitgeber 4 (4 h after the beginning of the light period) with the TRIzol method according to the 7 manufacturer (Thermo Fisher Scientific). .. For cDNA synthesis, 1 μg of total RNA was used in a 8 10 μL SuperScript III Reverse Transcriptase reaction (Thermo Fisher Scientific). ..

    Polymerase Chain Reaction:


    SYBR Green Assay:




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