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Becton Dickinson superscript reverse transcriptase kit
Superscript Reverse Transcriptase Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+ii+reverse+transcriptase/superscript+reverse+transcriptase+kit/pmc03586561-467-39-43
Average 90 stars, based on 1 article reviews
superscript reverse transcriptase kit - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Microarray:

Article Title: Rapid Mobilization of Membrane Lipids in Wheat Leaf-Sheaths during Incompatible Interactions with Hessian Fly
Article Snippet: .. Microarray data were deposited to the data base of National Center for Biotechnology Information (NCBI) with accession number GSE34445 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc= GSE34445 ). qPCR analysis DNase-treated total RNA was used for cDNA synthesis using random hexamers with a superscript reverse transcriptase kit (BD Biosciences, San Jose, CA). .. Samples were then treated with RNase H (Invitrogen). cDNA was quantified on a Nanodrop ND-1000 (NanoDrop Technologies Inc., Wilmington, DE) spectrophotometer and samples were diluted to 15 ng/μl to ensure equal amounts of cDNA template for quantification of mRNA abundance. qPCR was performed with iQ SYBR Green Supermix on a iCycler real time detection system (Bio-Rad, Hercules, CA).

Real-time Polymerase Chain Reaction:

Article Title: Rapid Mobilization of Membrane Lipids in Wheat Leaf-Sheaths during Incompatible Interactions with Hessian Fly
Article Snippet: .. Microarray data were deposited to the data base of National Center for Biotechnology Information (NCBI) with accession number GSE34445 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc= GSE34445 ). qPCR analysis DNase-treated total RNA was used for cDNA synthesis using random hexamers with a superscript reverse transcriptase kit (BD Biosciences, San Jose, CA). .. Samples were then treated with RNase H (Invitrogen). cDNA was quantified on a Nanodrop ND-1000 (NanoDrop Technologies Inc., Wilmington, DE) spectrophotometer and samples were diluted to 15 ng/μl to ensure equal amounts of cDNA template for quantification of mRNA abundance. qPCR was performed with iQ SYBR Green Supermix on a iCycler real time detection system (Bio-Rad, Hercules, CA).

cDNA Synthesis:

Article Title: Rapid Mobilization of Membrane Lipids in Wheat Leaf-Sheaths during Incompatible Interactions with Hessian Fly
Article Snippet: .. Microarray data were deposited to the data base of National Center for Biotechnology Information (NCBI) with accession number GSE34445 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc= GSE34445 ). qPCR analysis DNase-treated total RNA was used for cDNA synthesis using random hexamers with a superscript reverse transcriptase kit (BD Biosciences, San Jose, CA). .. Samples were then treated with RNase H (Invitrogen). cDNA was quantified on a Nanodrop ND-1000 (NanoDrop Technologies Inc., Wilmington, DE) spectrophotometer and samples were diluted to 15 ng/μl to ensure equal amounts of cDNA template for quantification of mRNA abundance. qPCR was performed with iQ SYBR Green Supermix on a iCycler real time detection system (Bio-Rad, Hercules, CA).

Article Title: Rapid Mobilization of Membrane Lipids in Wheat Leaf-Sheaths during Incompatible Interactions with Hessian Fly
Article Snippet: Microarray data were deposited to the data base of National Center for Biotechnology Information (NCBI) with accession number GSE34445 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc= GSE34445 ). .. DNase-treated total RNA was used for cDNA synthesis using random hexamers with a superscript reverse transcriptase kit (BD Biosciences, San Jose, CA). .. Samples were then treated with RNase H (Invitrogen). cDNA was quantified on a Nanodrop ND-1000 (NanoDrop Technologies Inc., Wilmington, DE) spectrophotometer and samples were diluted to 15 ng/μl to ensure equal amounts of cDNA template for quantification of mRNA abundance. qPCR was performed with iQ SYBR Green Supermix on a iCycler real time detection system (Bio-Rad, Hercules, CA).

Reverse Transcription:

Article Title: Rapid Mobilization of Membrane Lipids in Wheat Leaf-Sheaths during Incompatible Interactions with Hessian Fly
Article Snippet: .. Microarray data were deposited to the data base of National Center for Biotechnology Information (NCBI) with accession number GSE34445 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc= GSE34445 ). qPCR analysis DNase-treated total RNA was used for cDNA synthesis using random hexamers with a superscript reverse transcriptase kit (BD Biosciences, San Jose, CA). .. Samples were then treated with RNase H (Invitrogen). cDNA was quantified on a Nanodrop ND-1000 (NanoDrop Technologies Inc., Wilmington, DE) spectrophotometer and samples were diluted to 15 ng/μl to ensure equal amounts of cDNA template for quantification of mRNA abundance. qPCR was performed with iQ SYBR Green Supermix on a iCycler real time detection system (Bio-Rad, Hercules, CA).

Article Title: Rapid Mobilization of Membrane Lipids in Wheat Leaf-Sheaths during Incompatible Interactions with Hessian Fly
Article Snippet: Microarray data were deposited to the data base of National Center for Biotechnology Information (NCBI) with accession number GSE34445 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc= GSE34445 ). .. DNase-treated total RNA was used for cDNA synthesis using random hexamers with a superscript reverse transcriptase kit (BD Biosciences, San Jose, CA). .. Samples were then treated with RNase H (Invitrogen). cDNA was quantified on a Nanodrop ND-1000 (NanoDrop Technologies Inc., Wilmington, DE) spectrophotometer and samples were diluted to 15 ng/μl to ensure equal amounts of cDNA template for quantification of mRNA abundance. qPCR was performed with iQ SYBR Green Supermix on a iCycler real time detection system (Bio-Rad, Hercules, CA).

other:

Article Title: Rapid Mobilization of Membrane Lipids in Wheat Leaf Sheaths During Incompatible Interactions with Hessian Fly
Article Snippet: DNase-treated total RNA was used for cDNA synthesis using random hexamers with a superscript reverse-transcriptase kit (BD Biosciences, San Jose, CA, U.S.A.).



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