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superscript ii reverse transcriptase  (TaKaRa)


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    Structured Review

    TaKaRa superscript ii reverse transcriptase
    Superscript Ii Reverse Transcriptase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 725 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+ii+reverse+transcriptase/PrimeScript+II+Reverse+Transcriptase/pmc12664801-200-12-16
    Average 96 stars, based on 725 article reviews
    superscript ii reverse transcriptase - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Circ-ZBTB38 as an oncogenic circular RNA: Mechanisms in disrupting RalGAP complexes and its clinical value in melanoma.
    Article Snippet: Objective: Circular RNAs (circRNAs) hold significant potential both in the regulation of tumor progression and in clinical applications for tumors.. This study aims to investigate the functional mechanism of circ-ZBTB38 in melanoma progression as well as its potential clinical application value.. Methods: Differentially expressed circRNAs in melanoma were screened from the GSE138412 dataset and validated by circBase, qPCR, and Sanger sequencing.

    Article Title: SIM: Discovery of novel RNA-targeting argonautes by self-iterative learning from scarce data
    Article Snippet: Total RNA was extracted using the TRIzol reagent according to the manufacturer’s protocol (Invitrogen). .. The quality and yield of the extracted RNA were analyzed using agarose gel electrophoresis and spectrophotometry, respectively. cDNAs were synthesized from the total RNA (1 mg) using PrimeScript II Reverse Transcriptase (Takara). .. Then, qRT-PCR was performed using TB Green Prime Ex Taq II and the standard protocols of the Takara 3-step instrument (Roche LightCycler 480).

    Article Title: Targeting notch signaling to restore neural development and behavior in mouse models of ASD
    Article Snippet: Total RNA was isolated using RNAiso Plus (Takara, Kyoto, Japan), followed by DNase treatment with Turbo DNase (Ambion, Austin, TX, USA) to remove contaminating DNA. .. Two micrograms of total RNA were reverse transcribed using random hexamers and PrimeScript II Reverse Transcriptase (Takara). .. Quantitative PCR (qPCR) was performed on a StepOnePlus qPCR system (Applied Biosystems, Foster City, CA, USA) using SYBR Green Master Mix (Applied Biosystems) and the comparative CT method.

    Article Title: U2AF1 modulates alternative exon selection and guards zygotic splicing activation in mouse preimplantation embryogenesis.
    Article Snippet: Oocytes and embryos were collected and lysed directly with 0.2% Triton X100 supplied with 4 IU recombinant RNase inhibitor (RRI, Takara, 2313). .. AR TIC LE IN PR ES S ARTICLE IN PRESS The oocytic and embryonic RNAs were reverse-transcribed with random primers employing PrimeScriptTM II Reverse Transcriptase (Takara, 2690), according to the manufacturer’s instructions. .. The cDNAs were subjected to qPCR using Universal SYBR Green Fast qPCR Mix (ABclonal, RK21203) and a Bio-Rad CFX96 Touch Real-Time PCR system.

    Article Title: OsDi19-1 Acts Downstream of OsABI5 to Negatively Regulate ABA-Inhibited Seed Germination and Seedling Growth in Rice.
    Article Snippet: Seeds or seedlings were ground into powder in liquid nitrogen, and total RNA was extracted using the ZYMO RNA extraction kit (ZYMO Research, R2024). .. The RNA samples were then reverse transcribed into cDNA using dNTPs (TaKaRa, 4019), Oligo (dT)18 primer (TaKaRa, 3806), RNase inhibitor (TaKaRa, 2313 A), and PrimeScriptTM II Reverse Transcriptase (TaKaRa, 2690 A). ..

    Article Title: The landscape and regulatory potential of eccDNAs in mammalian preimplantation embryos.
    Article Snippet: .. Reverse transcription was performed using PrimeScript II Reverse Transcriptase (Takara) according to the manufacturer’s instructions. .. Quantitative PCR analysis was carried out with Power SYBR Green PCR Master Mix (Applied Biosystems) on an Applied Biosystems Real-Time PCR system.

    Article Title: Targeting notch signaling to restore neural development and behavior in mouse models of ASD.
    Article Snippet: Total RNA was isolated using RNAiso Plus (Takara, Kyoto, Japan), followed by DNase treatment with Turbo DNase (Ambion, Austin, TX, USA) to remove contaminating DNA. .. Two micrograms of total RNA were reverse transcribed using random hexamers and PrimeScript II Reverse Transcriptase (Takara). .. Nature Communications | (2026) 17:2587 12 Quantitative PCR (qPCR) was performed on a StepOnePlus qPCR system (Applied Biosystems, Foster City, CA, USA) using SYBR Green Master Mix (Applied Biosystems) and the comparative CT method.

    Agarose Gel Electrophoresis:

    Article Title: SIM: Discovery of novel RNA-targeting argonautes by self-iterative learning from scarce data
    Article Snippet: Total RNA was extracted using the TRIzol reagent according to the manufacturer’s protocol (Invitrogen). .. The quality and yield of the extracted RNA were analyzed using agarose gel electrophoresis and spectrophotometry, respectively. cDNAs were synthesized from the total RNA (1 mg) using PrimeScript II Reverse Transcriptase (Takara). .. Then, qRT-PCR was performed using TB Green Prime Ex Taq II and the standard protocols of the Takara 3-step instrument (Roche LightCycler 480).

    Spectrophotometry:

    Article Title: SIM: Discovery of novel RNA-targeting argonautes by self-iterative learning from scarce data
    Article Snippet: Total RNA was extracted using the TRIzol reagent according to the manufacturer’s protocol (Invitrogen). .. The quality and yield of the extracted RNA were analyzed using agarose gel electrophoresis and spectrophotometry, respectively. cDNAs were synthesized from the total RNA (1 mg) using PrimeScript II Reverse Transcriptase (Takara). .. Then, qRT-PCR was performed using TB Green Prime Ex Taq II and the standard protocols of the Takara 3-step instrument (Roche LightCycler 480).

    Synthesized:

    Article Title: SIM: Discovery of novel RNA-targeting argonautes by self-iterative learning from scarce data
    Article Snippet: Total RNA was extracted using the TRIzol reagent according to the manufacturer’s protocol (Invitrogen). .. The quality and yield of the extracted RNA were analyzed using agarose gel electrophoresis and spectrophotometry, respectively. cDNAs were synthesized from the total RNA (1 mg) using PrimeScript II Reverse Transcriptase (Takara). .. Then, qRT-PCR was performed using TB Green Prime Ex Taq II and the standard protocols of the Takara 3-step instrument (Roche LightCycler 480).



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