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Fisher Scientific superscript ii reverse transcriptase
Superscript Ii Reverse Transcriptase, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+ii+reverse+transcriptase/m+mlv+reverse+transcriptase/pmc12904577-326-5-9
Average 86 stars, based on 1 article reviews
superscript ii reverse transcriptase - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Synthesized:

Article Title: A single dose of purified human antibody from transchromosomic bovines mitigates aerosolized Venezuelan equine encephalitis virus disease in cynomolgus macaques.
Article Snippet: .. cDNA was synthesized from viral RNA using InvitrogenTM M-MLV Reverse Transcriptase (Fisher Scientific) in 20 μL reactions with either 100ng of total RNA from tissue-type samples or 5 μL of total RNA prep (1/8 of total RNA isolation volume) from liquid-type samples such as serum plasma. ..

Reverse Transcription:

Article Title: A single dose of purified human antibody from transchromosomic bovines mitigates aerosolized Venezuelan equine encephalitis virus disease in cynomolgus macaques.
Article Snippet: .. cDNA was synthesized from viral RNA using InvitrogenTM M-MLV Reverse Transcriptase (Fisher Scientific) in 20 μL reactions with either 100ng of total RNA from tissue-type samples or 5 μL of total RNA prep (1/8 of total RNA isolation volume) from liquid-type samples such as serum plasma. ..

Article Title: Gasdermin D Cleavage and Cytokine Release, Indicative of Pyroptotic Cell Death, Induced by Ophiobolin A in Breast Cancer Cell Lines.
Article Snippet: .. Total RNA was isolated from cells treated with or without OpA (1 μM) for 6 h, using TRIzol reagent (15596018, Ambion, Fisher Scientific) according to the manufacturer’s protocol. cDNA was generated using 250 ng of RNA with MultiScribe reverse transcriptase (4319983, Fisher Scientific) according to the manufacturer’s protocol. .. The synthesized cDNA was used to perform real-time quantitative PCR using qPCR blue mix (AZ2305, Azura Genomics, Raynham, MA, USA) by Quantstudio5 system (Applied Biosciences, Fisher Scientific).

Article Title: miRNA 183 Knockout Alters Cone Subtype Distribution, Transcriptional Activity and ERG Signals in the Tetrachromatic Zebrafish Visual System
Article Snippet: RNA concentration and quality were measured using a NanoDrop ND-2000 spectrophotometer (Thermal Fisher Scientific, Waltham, MA, USA). qRT-PCR was performed using the ViiA 7 real-time PCR system (Applied Biosystems, Carlsbad, CA, USA) to detect photoreceptor-related gene expression. .. M-MLV reverse transcriptase (28025021, Thermal Fisher Scientific, Inc.) was used to reverse transcribe 1 μg total RNA to cDNA according to the manufacturer’s protocol. .. An amount of 50 ng cDNA was mixed with PowerTrackTM SYBR Green Master Mix (A46012, Thermal Fisher Scientific, Waltham, MA, USA) and photoreceptor-related gene primers ( ) at a final concentration of 1 μM to produce a 15 μL reaction mixture.

Article Title: Recurrent mutations in the stress regulator Cap1 reveal a trade-off between azole resistance and oxidative stress response in Candida albicans
Article Snippet: .. cDNA was prepared using the SuperScript II Reverse Transcriptase (Fisher Scientific) according to the manufacturer’s instructions with oligo dT primers and 100 ng of RNA. cDNA was then diluted 1:10 with nuclease-free water for qPCR measurement. .. Realtime qPCR was conducted using the PowerUp SYBR Green Master Mix (Applied Biosystems) according to the manufacturer’s instructions to measure cDNA.

Article Title: CFIm25-dependent alternative polyadenylation in AKT2 mRNA programs macrophage polarization
Article Snippet: Anchored Oligo dT (22) primer , IDT , Cat# 51-01-15-09. .. Superscript III Reverse Transcriptase , Fisher Scientific , Cat# 18-080-093. .. Trypan Blue staining solution (0.4%) , Thermo Fisher Scientific , Cat# 15250061.

Article Title: miRNA 183 Knockout Alters Cone Subtype Distribution, Transcriptional Activity and ERG Signals in the Tetrachromatic Zebrafish Visual System.
Article Snippet: RNA concentration and quality were measured using a NanoDrop ND-2000 spectrophotometer (Thermal Fisher Scientific, Waltham, MA, USA). qRT-PCR was performed using the ViiA 7 real-time PCR system (Applied Biosystems, Carlsbad, CA, USA) to detect photoreceptor-related gene expression. .. M-MLV reverse transcriptase (28025021, Thermal Fisher Scientific, Inc.) was used to reverse transcribe 1 μg total RNA to cDNA according to the manufacturer’s protocol. .. An amount of 50 ng cDNA was mixed with PowerTrackTM SYBR Green Master Mix (A46012, Thermal Fisher Scientific, Waltham, MA, USA) and photoreceptor-related gene primers (Supplementary Table S1) at a final concentration of 1 μM to produce a 15 μL reaction mixture.

Article Title: ThermiQuant™ MegaScan: High-throughput isothermal reactor with quantitative colorimetric readout for paper-based nucleic acid amplification tests
Article Snippet: To prepare 1,000 μL of 2× LAMP mix, the solution was prepared by combining 100 μL KCl (1,000 mM; Sigma-Aldrich, P9541), 160 μL MgSO 4 (100 mM; Sigma-Aldrich, M2773), 280 μL dNTP mix (10 mM; Fisher Scientific, FERR0182), 2.8 μL dUTP (100 mM; Fisher Scientific, FERR0133), 0.4 μL Antarctic Thermolabile UDG (1 U/μL; New England Biolabs, M0372S), 5.4 μL Bst 2.0 DNA polymerase (120 U/μL; New England Biolabs, M0537M), 20 μL phenol red (25 mM; Sigma-Aldrich, P3532), 100 μL Tween-20 (20%; Sigma-Aldrich, P9416), and 331.4 μL nuclease-free water (Fisher Scientific, 43-879-36). .. For diluted NP swab samples containing SARS-CoV-2, this formulation was modified to enable reverse transcription by replacing the 40 μL of RO water with an equivalent volume of WarmStart RTx Reverse Transcriptase (Fisher Scientific, 43-879-36). .. To prepare 200 μL of the LAMP final master mix, 125 μL of the 2× LAMP solution was combined with 25 μL 10× primer mix (16 μM FIP/BIP, 2 μM F3/B3, and 4 μM LF/LB; final concentrations 1.6 μM FIP/BIP, 0.2 μM F3/B3, 0.4 μM LF/LB; see Table S6), 0.67 μL Bst 2.0 DNA polymerase, 1 μL betaine (5 M; Sigma-Aldrich, B03005VL), 3.13 μL bovine serum albumin (BSA) (40 mg/mL; Sigma-Aldrich, A2153), 36.0 μL trehalose (1.75 M; Thermo Scientific Chemicals, 182550250), and 9.2 μL nuclease-free water.

Article Title: Direct Virus-Bacteria Binding Enhances Streptococcus equi subsp. zooepidemicus Colonisation and Bacterial-Driven Immune Activation During H3N8 Equine Influenza A Virus Co-Infection
Article Snippet: .. Total RNA (0.5–1 μg) was reverse-transcribed into complementary DNA (cDNA) using the SuperScriptTM IV Reverse Transcriptase kit (Fisher Scientific, UK). .. Expression of host genes was quantified by RT-qPCR using a CFX Connect Real-Time PCR Detection System (Bio-Rad) with the SensiFAST SYBR No-ROX Kit (Bioline, UK).

Isolation:

Article Title: A single dose of purified human antibody from transchromosomic bovines mitigates aerosolized Venezuelan equine encephalitis virus disease in cynomolgus macaques.
Article Snippet: .. cDNA was synthesized from viral RNA using InvitrogenTM M-MLV Reverse Transcriptase (Fisher Scientific) in 20 μL reactions with either 100ng of total RNA from tissue-type samples or 5 μL of total RNA prep (1/8 of total RNA isolation volume) from liquid-type samples such as serum plasma. ..

Article Title: Gasdermin D Cleavage and Cytokine Release, Indicative of Pyroptotic Cell Death, Induced by Ophiobolin A in Breast Cancer Cell Lines.
Article Snippet: .. Total RNA was isolated from cells treated with or without OpA (1 μM) for 6 h, using TRIzol reagent (15596018, Ambion, Fisher Scientific) according to the manufacturer’s protocol. cDNA was generated using 250 ng of RNA with MultiScribe reverse transcriptase (4319983, Fisher Scientific) according to the manufacturer’s protocol. .. The synthesized cDNA was used to perform real-time quantitative PCR using qPCR blue mix (AZ2305, Azura Genomics, Raynham, MA, USA) by Quantstudio5 system (Applied Biosciences, Fisher Scientific).

Clinical Proteomics:

Article Title: A single dose of purified human antibody from transchromosomic bovines mitigates aerosolized Venezuelan equine encephalitis virus disease in cynomolgus macaques.
Article Snippet: .. cDNA was synthesized from viral RNA using InvitrogenTM M-MLV Reverse Transcriptase (Fisher Scientific) in 20 μL reactions with either 100ng of total RNA from tissue-type samples or 5 μL of total RNA prep (1/8 of total RNA isolation volume) from liquid-type samples such as serum plasma. ..

Generated:

Article Title: Gasdermin D Cleavage and Cytokine Release, Indicative of Pyroptotic Cell Death, Induced by Ophiobolin A in Breast Cancer Cell Lines.
Article Snippet: .. Total RNA was isolated from cells treated with or without OpA (1 μM) for 6 h, using TRIzol reagent (15596018, Ambion, Fisher Scientific) according to the manufacturer’s protocol. cDNA was generated using 250 ng of RNA with MultiScribe reverse transcriptase (4319983, Fisher Scientific) according to the manufacturer’s protocol. .. The synthesized cDNA was used to perform real-time quantitative PCR using qPCR blue mix (AZ2305, Azura Genomics, Raynham, MA, USA) by Quantstudio5 system (Applied Biosciences, Fisher Scientific).

Real-time Polymerase Chain Reaction:

Article Title: Recurrent mutations in the stress regulator Cap1 reveal a trade-off between azole resistance and oxidative stress response in Candida albicans
Article Snippet: .. cDNA was prepared using the SuperScript II Reverse Transcriptase (Fisher Scientific) according to the manufacturer’s instructions with oligo dT primers and 100 ng of RNA. cDNA was then diluted 1:10 with nuclease-free water for qPCR measurement. .. Realtime qPCR was conducted using the PowerUp SYBR Green Master Mix (Applied Biosystems) according to the manufacturer’s instructions to measure cDNA.

Formulation:

Article Title: ThermiQuant™ MegaScan: High-throughput isothermal reactor with quantitative colorimetric readout for paper-based nucleic acid amplification tests
Article Snippet: To prepare 1,000 μL of 2× LAMP mix, the solution was prepared by combining 100 μL KCl (1,000 mM; Sigma-Aldrich, P9541), 160 μL MgSO 4 (100 mM; Sigma-Aldrich, M2773), 280 μL dNTP mix (10 mM; Fisher Scientific, FERR0182), 2.8 μL dUTP (100 mM; Fisher Scientific, FERR0133), 0.4 μL Antarctic Thermolabile UDG (1 U/μL; New England Biolabs, M0372S), 5.4 μL Bst 2.0 DNA polymerase (120 U/μL; New England Biolabs, M0537M), 20 μL phenol red (25 mM; Sigma-Aldrich, P3532), 100 μL Tween-20 (20%; Sigma-Aldrich, P9416), and 331.4 μL nuclease-free water (Fisher Scientific, 43-879-36). .. For diluted NP swab samples containing SARS-CoV-2, this formulation was modified to enable reverse transcription by replacing the 40 μL of RO water with an equivalent volume of WarmStart RTx Reverse Transcriptase (Fisher Scientific, 43-879-36). .. To prepare 200 μL of the LAMP final master mix, 125 μL of the 2× LAMP solution was combined with 25 μL 10× primer mix (16 μM FIP/BIP, 2 μM F3/B3, and 4 μM LF/LB; final concentrations 1.6 μM FIP/BIP, 0.2 μM F3/B3, 0.4 μM LF/LB; see Table S6), 0.67 μL Bst 2.0 DNA polymerase, 1 μL betaine (5 M; Sigma-Aldrich, B03005VL), 3.13 μL bovine serum albumin (BSA) (40 mg/mL; Sigma-Aldrich, A2153), 36.0 μL trehalose (1.75 M; Thermo Scientific Chemicals, 182550250), and 9.2 μL nuclease-free water.

Modification:

Article Title: ThermiQuant™ MegaScan: High-throughput isothermal reactor with quantitative colorimetric readout for paper-based nucleic acid amplification tests
Article Snippet: To prepare 1,000 μL of 2× LAMP mix, the solution was prepared by combining 100 μL KCl (1,000 mM; Sigma-Aldrich, P9541), 160 μL MgSO 4 (100 mM; Sigma-Aldrich, M2773), 280 μL dNTP mix (10 mM; Fisher Scientific, FERR0182), 2.8 μL dUTP (100 mM; Fisher Scientific, FERR0133), 0.4 μL Antarctic Thermolabile UDG (1 U/μL; New England Biolabs, M0372S), 5.4 μL Bst 2.0 DNA polymerase (120 U/μL; New England Biolabs, M0537M), 20 μL phenol red (25 mM; Sigma-Aldrich, P3532), 100 μL Tween-20 (20%; Sigma-Aldrich, P9416), and 331.4 μL nuclease-free water (Fisher Scientific, 43-879-36). .. For diluted NP swab samples containing SARS-CoV-2, this formulation was modified to enable reverse transcription by replacing the 40 μL of RO water with an equivalent volume of WarmStart RTx Reverse Transcriptase (Fisher Scientific, 43-879-36). .. To prepare 200 μL of the LAMP final master mix, 125 μL of the 2× LAMP solution was combined with 25 μL 10× primer mix (16 μM FIP/BIP, 2 μM F3/B3, and 4 μM LF/LB; final concentrations 1.6 μM FIP/BIP, 0.2 μM F3/B3, 0.4 μM LF/LB; see Table S6), 0.67 μL Bst 2.0 DNA polymerase, 1 μL betaine (5 M; Sigma-Aldrich, B03005VL), 3.13 μL bovine serum albumin (BSA) (40 mg/mL; Sigma-Aldrich, A2153), 36.0 μL trehalose (1.75 M; Thermo Scientific Chemicals, 182550250), and 9.2 μL nuclease-free water.



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