Review

superscript ii rnase h2 reverse transcriptase kit (Thermo Fisher)

Thermo Fisher is a verified supplier

Thermo Fisher manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Thermo Fisher superscript ii rnase h2 reverse transcriptase kit
Superscript Ii Rnase H2 Reverse Transcriptase Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+ii+reverse+transcriptase/Ribonuclease+A/pmc12530098-190-21-28
Average 99 stars, based on 1 article reviews

superscript ii rnase h2 reverse transcriptase kit - by Bioz Stars, 2026-09

99/100 stars

Images

Immunoprecipitation:

Article Title: RNA-binding proteins TDP-43 and FUS promote R-loop resolution and regulate transcription termination
Article Snippet: .. RNase-sensitivity analysis for R-loops was carried out by adding 50 U of RNase H (Invitrogen, 18021–014) in 10X RNase H buffer (75 mM KCl, 50 mM Tris pH8.3, 3 mM MgCl2, 10 mM DTT) with 4% glycerol and 20 μg/ml BSA prior to immunoprecipitation; the RNase H treatment was performed for 2 h at 37 °C. ..

Incubation:

Article Title: Therapeutic implications of targeting cancer testis antigen MAGEA1 in cervical cancer
Article Snippet: .. RNase A (50 μ l; 100 μ g/ml; Thermo Fisher Scientific, Inc.) was added and incubated at 37°C water bath for 20 min. Then, the cells were treated with 5 μ l propidium iodide (10 mg/ml, Invitrogen; Thermo Fisher Scientific, Inc.) seeded in 96 well plates with density at 1×10 6 cells/well and incubated at 4°C in dark until flow cytometry readings. .. Cell cycle distribution was measured by flow cytometry (CytoFLEX V0-B3-R2 Flow Cytometer; Beckman Coulter, Inc.).

Flow Cytometry:

Extraction:

Article Title: The nuclear cap-binding complex safeguards stress-resistant protein synthesis and proliferation of stem cells
Article Snippet: .. Briefly, the cells treated with cycloheximide (100 μg/ml; Merck Sigma Millipore) were lysed in a polysome cell extraction buffer [50 mM MOPS (Merck Sigma Millipore), 15 mM MgCl 2 (Merck Sigma Millipore), 150 mM NaCl, cycloheximide, 0.5% (v/v) Triton X-100 (100 μg/ml), heparin sodium salt (1 mg/ml; Merck Sigma Millipore), RiboLock RNase inhibitor (0.2 U/μl; Thermo Fisher Scientific), 2 mM PMSF, and 1 mM benzamidine hydrochloride]. ..