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Magen Biotechnology Co Ltd rtl lysis buffer
Rtl Lysis Buffer, supplied by Magen Biotechnology Co Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rtl+buffer/buffer+lysis+rl/pm40650228-207-8-11
Average 86 stars, based on 1 article reviews
rtl lysis buffer - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

Preserving:

Article Title: Optimizing RNA quality in cryopreserved tissues without preservatives: impact of preservatives, thawing methods and tissue aliquot sizes.
Article Snippet: .. The preservation solution (when present) was carefully discarded and replaced with 750 μL of RL lysis buffer (Magen Biotechnology, China). ..

Article Title: Optimizing RNA quality in cryopreserved tissues without preservatives: impact of preservatives, thawing methods and tissue aliquot sizes
Article Snippet: .. The preservation solution (when present) was carefully discarded and replaced with 750 μL of RL lysis buffer (Magen Biotechnology, China). ..

Lysis:

Article Title: Optimizing RNA quality in cryopreserved tissues without preservatives: impact of preservatives, thawing methods and tissue aliquot sizes.
Article Snippet: .. The preservation solution (when present) was carefully discarded and replaced with 750 μL of RL lysis buffer (Magen Biotechnology, China). ..

Article Title: Analysis of TAS2R bitter taste receptors expression in the nasal epithelium of asthma patients by nextgeneration sequencing
Article Snippet: .. Клетки назального эпителия получали с помощью браш-биопсии из нижней носовой раковины, после чего немедленно помещали в RTL Lysis Buffer (Guangzhou Magen Biotechnology Co., Ltd., КНР) и замораживали при -80°C. .. Общую РНК выделяли коммерческими наборами HiPure Total RNA Kit (Guangzhou Magen Biotechnology Co., Ltd., КНР) согласно протоколу производителя.

Article Title: Optimizing RNA quality in cryopreserved tissues without preservatives: impact of preservatives, thawing methods and tissue aliquot sizes.
Article Snippet: .. The smashed tissue aliquots were weighed, with 10–30 mg aliquots randomly allocated to four groups: (A) neat control group without preservatives; (B) RL lysis buffer (Magen Biotechnology, China); (C) RNALater stabilization solution (Beyotime Biotechnology, China); and (D) TRIzol reagent (Thermo Fisher Scientific, USA). .. Since the Hipure Total RNA Mini Kit (Magen Biotechnology, China) protocol requires tissue lysis in RL buffer, this solution was also used as a (2) thawing on ice for small aliquots (≤ 100 mg) or at -20 °C for larger samples, and (3) minimizing freeze‒thaw cycles, despite observed variations among species.

Article Title: Optimizing RNA quality in cryopreserved tissues without preservatives: impact of preservatives, thawing methods and tissue aliquot sizes
Article Snippet: .. The preservation solution (when present) was carefully discarded and replaced with 750 μL of RL lysis buffer (Magen Biotechnology, China). ..

Article Title: Optimizing RNA quality in cryopreserved tissues without preservatives: impact of preservatives, thawing methods and tissue aliquot sizes
Article Snippet: .. The smashed tissue aliquots were weighed, with 10–30 mg aliquots randomly allocated to four groups: (A) neat control group without preservatives; (B) RL lysis buffer (Magen Biotechnology, China); (C) RNALater stabilization solution (Beyotime Biotechnology, China); and (D) TRIzol reagent (Thermo Fisher Scientific, USA). .. Since the Hipure Total RNA Mini Kit (Magen Biotechnology, China) protocol requires tissue lysis in RL buffer, this solution was also used as a preservative.

Article Title: Nodal Expansion, Tumor Infiltration and Exhaustion of Neoepitope-Specific Th Cells After Prophylactic Peptide Vaccination and Anti-CTLA4 Therapy in Mouse Melanoma B16.
Article Snippet: the resulting cDNA samples (20 μL) were purified with MagPure A4 magnetic beads (Magen Biotechnology) at a 1:1.5 sample/beads ratio (v:v) and resuspended in 20 μL 10 mM TE buffer (10 mM Tris/HCL, 1 mM EDTA, pH8.5). .. The pellets were resuspended in 300 μL of RTL lysis buffer (Magen Biotechnology) and processed with the HiPure Total RNA Kit (Magen Biotechnology) for RNA extraction. .. Next, TCRβ cDNA was generated from 20–40 ng of the eluted RNA with a primer for the TRBV constant region and QScribe III revertase (provided by Ekaterina Barsova) for 30 min at 42 ◦C. the resulting cDNA samples (20 μL) were purified with MagPure A4 magnetic beads (Magen Biotechnology) at a 1:1.5 sample/beads ratio (v:v) and resuspended in 20 μL 10 mM TE buffer (10 mM Tris/HCL, 1 mM EDTA, pH8.5).

Control:

Article Title: Optimizing RNA quality in cryopreserved tissues without preservatives: impact of preservatives, thawing methods and tissue aliquot sizes.
Article Snippet: .. The smashed tissue aliquots were weighed, with 10–30 mg aliquots randomly allocated to four groups: (A) neat control group without preservatives; (B) RL lysis buffer (Magen Biotechnology, China); (C) RNALater stabilization solution (Beyotime Biotechnology, China); and (D) TRIzol reagent (Thermo Fisher Scientific, USA). .. Since the Hipure Total RNA Mini Kit (Magen Biotechnology, China) protocol requires tissue lysis in RL buffer, this solution was also used as a (2) thawing on ice for small aliquots (≤ 100 mg) or at -20 °C for larger samples, and (3) minimizing freeze‒thaw cycles, despite observed variations among species.

Article Title: Optimizing RNA quality in cryopreserved tissues without preservatives: impact of preservatives, thawing methods and tissue aliquot sizes
Article Snippet: .. The smashed tissue aliquots were weighed, with 10–30 mg aliquots randomly allocated to four groups: (A) neat control group without preservatives; (B) RL lysis buffer (Magen Biotechnology, China); (C) RNALater stabilization solution (Beyotime Biotechnology, China); and (D) TRIzol reagent (Thermo Fisher Scientific, USA). .. Since the Hipure Total RNA Mini Kit (Magen Biotechnology, China) protocol requires tissue lysis in RL buffer, this solution was also used as a preservative.

RNA Extraction:

Article Title: Nodal Expansion, Tumor Infiltration and Exhaustion of Neoepitope-Specific Th Cells After Prophylactic Peptide Vaccination and Anti-CTLA4 Therapy in Mouse Melanoma B16.
Article Snippet: the resulting cDNA samples (20 μL) were purified with MagPure A4 magnetic beads (Magen Biotechnology) at a 1:1.5 sample/beads ratio (v:v) and resuspended in 20 μL 10 mM TE buffer (10 mM Tris/HCL, 1 mM EDTA, pH8.5). .. The pellets were resuspended in 300 μL of RTL lysis buffer (Magen Biotechnology) and processed with the HiPure Total RNA Kit (Magen Biotechnology) for RNA extraction. .. Next, TCRβ cDNA was generated from 20–40 ng of the eluted RNA with a primer for the TRBV constant region and QScribe III revertase (provided by Ekaterina Barsova) for 30 min at 42 ◦C. the resulting cDNA samples (20 μL) were purified with MagPure A4 magnetic beads (Magen Biotechnology) at a 1:1.5 sample/beads ratio (v:v) and resuspended in 20 μL 10 mM TE buffer (10 mM Tris/HCL, 1 mM EDTA, pH8.5).



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