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rtl plus buffer  (Qiagen)


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    Structured Review

    Qiagen rtl plus buffer
    Rtl Plus Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 5292 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rtl+buffer/RNeasy+Plus+Mini+Kit/pm41922576-99-7-10
    Average 99 stars, based on 5292 article reviews
    rtl plus buffer - by Bioz Stars, 2026-10
    99/100 stars

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    Isolation:

    Article Title: Chimeric antigen receptor T cell targeting CD44E variant in HCC holds therapeutic potential
    Article Snippet: .. Total RNA was isolated using the RNeasy Plus Mini kit (Qiagen, cat. no. 74136) according to the manufacturer’s instructions. .. RNA concentration was quantified using a Nanodrop 2000 Spectrophotometer, and cDNA was synthesized from 1 μg of total RNA using SuperScript III First-Strand Synthesis System (Invitrogen, cat. no. 18080051).

    Article Title: IgG Glycosylation-Dependent CLEC7A Signaling Drives Podocyte Dysfunction in Lupus Nephritis
    Article Snippet: .. Briefly, total RNA was isolated from cultured podocytes using the RNeasy Plus Mini Kit (QIAGEN, catalog 74136). .. Purified total RNA (0.2–1 μg) was used to prepare cDNA through high-capacity RNA to c-DNA kit (Applied Biosystems, catalog 4368814).

    Article Title: Inducible chronic NAD deficiency in mice reveals multi-systemic phenotypical, metabolic, and transcriptional changes
    Article Snippet: .. RNA was isolated from cells and mouse tissues using TRIzol (Invitrogen, 15596026) or the Qiagen RNeasy kit (Qiagen, 74136) according to the manufacturers’ instruction. .. cDNA was synthesized using the Qiagen QuantiTect or the ABI High-Capacity cDNA Reverse Transcription kit.

    Reverse Transcription:

    Article Title: The DNMT1 CXXC domain senses CpG islands for DNA methylation inhibition
    Article Snippet: .. Total RNA was extracted using the RNeasy Plus Mini Kit (Qiagen, cat# 74136) and reverse transcribed into cDNA with the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, cat# 4368813). .. Quantitative PCR was performed in triplicate using the iTaq Universal SYBR Green Supermix (Bio-Rad, cat# 1725124) on a ViiA 7 Real-Time PCR System (Applied Biosystems).

    Article Title: Synergistic role of TFEB/TFE3 and mTORC1 in kidney cystogenesis and tumorigenesis.
    Article Snippet: Immunoreactive bands were detected by chemiluminescence and digitally acquired using an ImageQuant LAS4000 imaging system (GE Healthcare) or Amersham Imager 680 (Cytiva). .. RNA extraction, reverse transcription and quantitative PCR RNA samples from mouse kidneys were extracted using the RNeasy Plus Mini Kit (Qiagen cat no. 74136) according to the manufacturer’s instructions. .. cDNA was synthesized using the QuantiTect Reverse Transcription Kit (Qiagen Cat no. / ID.

    Article Title: Prenatal exposure to cannabidiol affects spermatogonial homeostasis and the production of spermatozoa in adulthood
    Article Snippet: .. RNA extraction and Reverse Transcription quantitative real-time PCR (RT-qPCR) AR TIC LE IN PR ES S RNA was extracted from frozen testes using the RNeasy Plus Mini Kit (Cat. No. 74136, QIAGEN) according to the manufacturer’s instructions. .. RNA concentration was determined by a Nanodrop spectrometer.

    RNA Extraction:

    Article Title: Synergistic role of TFEB/TFE3 and mTORC1 in kidney cystogenesis and tumorigenesis.
    Article Snippet: Immunoreactive bands were detected by chemiluminescence and digitally acquired using an ImageQuant LAS4000 imaging system (GE Healthcare) or Amersham Imager 680 (Cytiva). .. RNA extraction, reverse transcription and quantitative PCR RNA samples from mouse kidneys were extracted using the RNeasy Plus Mini Kit (Qiagen cat no. 74136) according to the manufacturer’s instructions. .. cDNA was synthesized using the QuantiTect Reverse Transcription Kit (Qiagen Cat no. / ID.

    Article Title: Prenatal exposure to cannabidiol affects spermatogonial homeostasis and the production of spermatozoa in adulthood
    Article Snippet: .. RNA extraction and Reverse Transcription quantitative real-time PCR (RT-qPCR) AR TIC LE IN PR ES S RNA was extracted from frozen testes using the RNeasy Plus Mini Kit (Cat. No. 74136, QIAGEN) according to the manufacturer’s instructions. .. RNA concentration was determined by a Nanodrop spectrometer.

    Real-time Polymerase Chain Reaction:

    Article Title: Synergistic role of TFEB/TFE3 and mTORC1 in kidney cystogenesis and tumorigenesis.
    Article Snippet: Immunoreactive bands were detected by chemiluminescence and digitally acquired using an ImageQuant LAS4000 imaging system (GE Healthcare) or Amersham Imager 680 (Cytiva). .. RNA extraction, reverse transcription and quantitative PCR RNA samples from mouse kidneys were extracted using the RNeasy Plus Mini Kit (Qiagen cat no. 74136) according to the manufacturer’s instructions. .. cDNA was synthesized using the QuantiTect Reverse Transcription Kit (Qiagen Cat no. / ID.

    Article Title: Prenatal exposure to cannabidiol affects spermatogonial homeostasis and the production of spermatozoa in adulthood
    Article Snippet: .. RNA extraction and Reverse Transcription quantitative real-time PCR (RT-qPCR) AR TIC LE IN PR ES S RNA was extracted from frozen testes using the RNeasy Plus Mini Kit (Cat. No. 74136, QIAGEN) according to the manufacturer’s instructions. .. RNA concentration was determined by a Nanodrop spectrometer.

    other:

    Article Title: Topoisomerase I inhibition yields a CD74 high /MHC high human microglial subtype with enhanced Aβ uptake.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER M-CSF R&D Cat.# 216-MC-010/CF EMEM ATCC Cat#: 30-2003 DMEM/F12 Thermo Fisher Cat.# 11320033 2X insulin-transferrin-selenite Thermo Fisher Cat.# 41400045 B27 Thermo Fisher Cat.# 17504044 N2 Thermo Fisher Cat.# 17502048 1X glutamax Thermo Fisher Cat.# 35050061 1X non-essential amino acids Thermo Fisher Cat.# 11140050 Monothioglycerol Sigma-Aldrich Cat.# M-6145 IL-34 R&D Cat.# 5265-IL-010/CF TGFβ1 STEMCELL Technologies Cat.# 100-0856 CD200 Bon Opus Biosciences Cat#: 50-203-1792 CX3CL1 R&D Systems Cat#: 365-FR-025/CF paraformaldehyde ThermoFisher Cat#: J61899-AP 20% Sucrose Invitrogen Cat#: 15503-022 ethylenediaminetetraacetic acid (EDTA) Sigma Cat#: ED4SS Triton X-100 Millipore Sigma, Cat#: 648466 aquamount Polysciences Inc. Cat#: 18606 RLT buffer Qiagen Cat#: 74136 2-Mercaptoethanol ThermoFisher Cat#:63689 P/S Gibco Cat#: 15140-122 Cytochalasin D Sigma-Aldrich Cat#: C8273 Trypsin Gen Clone Cat#:25-510F FCS Fisher Scientific Cat#: 10-438-026 Cell Staining buffer BioLegend Cat#: 420201 NaOH ThermoFisher Cat#: 63736.AK Critical commercial assays 10x Chromium Next GEM Single Cell 5′ Reagent Kits v2 (Dual Index) 10x Genomics, Pleasanton, CA Cat#: PN-1000263 TapeStation D5000 screening tapes and reagents Agilent, Santa Clara, CA Cat#: 5067-5588; 5067-5589 Qubit HS DNA quantification kit ThermoFisher Cat#: Q32851 STEMdiff Hematopoietic kit STEMCELL Technologies Cat#: 05310 STEMdiffTM Cerebral Organoid Kit STEMCELL Technologies Cat#: 08570, Cat#: 08571 MTT assay Invitrogen Cat#: M6494 Alamar Blue HS Thermo Fisher Cat#: DAL1100 Qiagen RNEasy mini plus kit Qiagen Cat#: 74136 BioRad iScript cDNA Synthesis kit BioRad Cat#:1708891 Qubit HS DNA quantification kit ThermoFisher Cat#: Q32851 Human Cytokine Proinflammatory Focused 15-Plex Discovery Assay Array (HDF15) Eve Technologies N/A Alamar Blue Assay ThermoFisher Cat#: DAL1025 Beta Amyloid (1–42), Aggregation Kit rPeptide A-1170-02 Deposited data AD Knowledge Portal: sc-RNA Sequencing HMC3 compound- treated https://www.synapse.org/Synapse: syn53124932 AD Knowledge Portal: sc-RNA Sequencing iMG compound- treated https://www.synapse.org/Synapse: syn53124932 (Continued on next page) 20 Cell Reports 45, 118031, October 27, 2026

    Purification:

    Article Title: Genetic inactivation of TRPM4 does not alter the temperature-dependent movement of mouse microglia
    Article Snippet: .. Total RNA was purified from mouse brain samples and primary mouse microglia obtained from WT or TRPM4KO mouse using the RNeasy Plus Mini kit (Qiagen) with DNase I (Sigma-Aldrich) treatment to eliminate genomic DNA according to the manufacturer’s protocols. .. cDNA was synthesized from total RNA (up to 1 μg) by reverse transcription using the SuperScript III first-strand synthesis system for RT-PCR (Invitrogen).

    Cell Culture:

    Article Title: IgG Glycosylation-Dependent CLEC7A Signaling Drives Podocyte Dysfunction in Lupus Nephritis
    Article Snippet: .. Briefly, total RNA was isolated from cultured podocytes using the RNeasy Plus Mini Kit (QIAGEN, catalog 74136). .. Purified total RNA (0.2–1 μg) was used to prepare cDNA through high-capacity RNA to c-DNA kit (Applied Biosystems, catalog 4368814).



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