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rt112  (ATCC)


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    ATCC rt112
    Rt112, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 758 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rt112/SV-HUC-1/pm42045965-85-1-8
    Average 97 stars, based on 758 article reviews
    rt112 - by Bioz Stars, 2026-11
    97/100 stars

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    Related Articles

    Virus:

    Article Title: Wireless electrostimulation implants enable sphincter neuromuscular improvement toward mixed urinary incontinence.
    Article Snippet: .. The Simian Virus 40-transformed Human Uroepithelial Cells line (SV-HUC-1) was obtained from the American Type Culture Collection (ATCC, USA) and maintained in an incubator with 5% CO2 at 37°C. .. The cells were cultured in high-glucose Dulbecco's Modified Eagle Medium (High-glucose DMEM, Gibco, USA) as the basic culture medium, supplemented with 10% fetal bovine serum (FBS, Gibco, USA), 100 U/ml streptomycin, and 100 μg/ml penicillin (Gibco, USA).

    Cell Culture:

    Article Title: VEGF-C maturation mediated by CircFOCAD-orchestrated CCBE1 and ADAM10 expression in nuclear-cytoplasmic synergy drives lymphatic metastasis.
    Article Snippet: .. Cell line and cell culture In this study, the T24, UM-UC-3, and 5637 cell lines, which are human BCa cell lines, and SV-HUC-1, which is a normal human bladder epithelial cell line, were obtained from the American Type Culture Collection in Manassas, Virginia, USA and tested negative for mycoplasma contamination. ..

    Article Title: Novel nanotherapeutic strategy targeting mechanical tumor microenvironment in bladder cancer
    Article Snippet: Following tissue collection, total RNA was extracted from the tumor using TRIzol reagent (15596018, Thermo Fisher Scientific, USA), reverse transcription was performed with the PrimeScript RT Reagent Kit (RR037A, Takara, Japan), and luciferase mRNA expression levels were quantified by qPCR. .. Cell Culture and Grouping The following cell lines were used in this study: mouse embryonic fibroblasts NIH-3T3 (CRL-1658, ATCC, USA), human bladder epithelial cells SV-HUC-1 (CRL-9520, ATCC, USA), mouse macrophages RAW264.7 (TIB-71, ATCC, USA), and mouse BC cells MB49 (CRL-1655, ATCC, USA). .. NIH-3T3, RAW264.7, and MB49 cells were cultured in high-glucose DMEM (11965092, Gibco, USA) supplemented with 10% FBS (10099141C, Gibco, USA), 100



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    Effect of berbamine on 5-ALA-induced PpIX accumulation. <t>RT112,</t> RT112 LTT, RT112 CSC (top), and J82, J82 LTT, J82 CSC (bottom) were treated with 5-ALA (0–100 µg/ml) and berbamine (no, ½ IC 50 , IC 50 ). PpIX fluorescence intensity was normalized to cell viability and is shown as individual values with mean as a line. Asterisks indicate significance compared to the sample without berbamine (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).
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    Effect of berbamine on 5-ALA-induced PpIX accumulation. <t>RT112,</t> RT112 LTT, RT112 CSC (top), and J82, J82 LTT, J82 CSC (bottom) were treated with 5-ALA (0–100 µg/ml) and berbamine (no, ½ IC 50 , IC 50 ). PpIX fluorescence intensity was normalized to cell viability and is shown as individual values with mean as a line. Asterisks indicate significance compared to the sample without berbamine (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).
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    Effect of berbamine on 5-ALA-induced PpIX accumulation. RT112, RT112 LTT, RT112 CSC (top), and J82, J82 LTT, J82 CSC (bottom) were treated with 5-ALA (0–100 µg/ml) and berbamine (no, ½ IC 50 , IC 50 ). PpIX fluorescence intensity was normalized to cell viability and is shown as individual values with mean as a line. Asterisks indicate significance compared to the sample without berbamine (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).

    Journal: Scientific Reports

    Article Title: Combining 5-ALA-PDT with berbamine as an in vitro multimodal therapy approach against bladder cancer cells

    doi: 10.1038/s41598-026-46092-x

    Figure Lengend Snippet: Effect of berbamine on 5-ALA-induced PpIX accumulation. RT112, RT112 LTT, RT112 CSC (top), and J82, J82 LTT, J82 CSC (bottom) were treated with 5-ALA (0–100 µg/ml) and berbamine (no, ½ IC 50 , IC 50 ). PpIX fluorescence intensity was normalized to cell viability and is shown as individual values with mean as a line. Asterisks indicate significance compared to the sample without berbamine (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).

    Article Snippet: RT112 (DSMZ, cat. no. ACC-418) and J82 (ATCC, cat. no. HTB-1) cell lines originate from transitional cell carcinoma of the human bladder.

    Techniques: Fluorescence

    Cell viability of RT112, RT112 LTT and RT112 CSC following 5-ALA-PDT treatment with increasing irradiation doses and varying concentrations of 5-ALA and berbamine. Bars represent weighted means ± SD from three independent experiments ( n = 3 × 6; controls without irradiation n = 3 × 36). Asterisks indicate significance of unweighted means compared to the irradiated control sample without 5-ALA C1.1-C1.4 (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).

    Journal: Scientific Reports

    Article Title: Combining 5-ALA-PDT with berbamine as an in vitro multimodal therapy approach against bladder cancer cells

    doi: 10.1038/s41598-026-46092-x

    Figure Lengend Snippet: Cell viability of RT112, RT112 LTT and RT112 CSC following 5-ALA-PDT treatment with increasing irradiation doses and varying concentrations of 5-ALA and berbamine. Bars represent weighted means ± SD from three independent experiments ( n = 3 × 6; controls without irradiation n = 3 × 36). Asterisks indicate significance of unweighted means compared to the irradiated control sample without 5-ALA C1.1-C1.4 (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).

    Article Snippet: RT112 (DSMZ, cat. no. ACC-418) and J82 (ATCC, cat. no. HTB-1) cell lines originate from transitional cell carcinoma of the human bladder.

    Techniques: Irradiation, Control

    Berbamine has cytotoxic effects in ROS generation, cell migration and apoptosis rate for cisplatin-resistant cell lines RT112 LTT and J82 LTT. Tert-butyl hydroperoxide (TBHP; 150 µM) served as positive control for determination of reactive oxygen species (ROS). Detailed data presentation for all cell lines are available in the supplementary section (Supplementary Figs. , and ). Data represent mean ± SD ( n = 3). Asterisks indicate significance relative to the control without berbamine (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).

    Journal: Scientific Reports

    Article Title: Combining 5-ALA-PDT with berbamine as an in vitro multimodal therapy approach against bladder cancer cells

    doi: 10.1038/s41598-026-46092-x

    Figure Lengend Snippet: Berbamine has cytotoxic effects in ROS generation, cell migration and apoptosis rate for cisplatin-resistant cell lines RT112 LTT and J82 LTT. Tert-butyl hydroperoxide (TBHP; 150 µM) served as positive control for determination of reactive oxygen species (ROS). Detailed data presentation for all cell lines are available in the supplementary section (Supplementary Figs. , and ). Data represent mean ± SD ( n = 3). Asterisks indicate significance relative to the control without berbamine (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).

    Article Snippet: RT112 (DSMZ, cat. no. ACC-418) and J82 (ATCC, cat. no. HTB-1) cell lines originate from transitional cell carcinoma of the human bladder.

    Techniques: Migration, Positive Control, Control