Journal: Clinical and Translational Medicine

Article Title: Multi‐omic profiling defines three distinct molecular subtypes of urothelial carcinoma with implications for precision therapy

doi: 10.1002/ctm2.70638

Figure Lengend Snippet: In vitro response to anti‐cancer drugs and RNA‐seq in urothelial cancer cell lines representative for the three bladder cancer clusters. Response to chemotherapy drugs (A) cisplatin and (B) gemcitabine, (C) EGFR/ERBB inhibitor gefitinib and (D) MAPK inhibitor cobimetinib. Drug response graphs are shown with the corresponding RNA‐seq data for EGFR/ERBB inhibitors (E) neratinib and (F) afatinib, (G) MAPK inhibitor trametinib and (H) FGFR inhibitor erdafitinib. Drug response graphs are furthermore given for the DNA methyltransferase inhibitor (I) decitabine and the ferroptosis activator (J) 1S,3R‐RSL‐3. Confluence was measured after 4 days using the IncuCyte for Cluster #1 cell lines UMUC3 and 639V, Cluster #2 cell lines 5637 and VMCUB1, and Cluster #3 cell lines RT112 and UMUC6. Points represent mean measurements for three independent biological replicates with error bars representing the SEM, with colour‐coding for the cell line/cluster assignment. Heatmaps show regulation of growth and repair, metabolism and immune and signalling pathways in the control versus treated cell line models. Tile colour corresponds to the relative activity of the gene signatures. (K) Pathway analysis showing western blot bands, analysing protein expression levels of EGFR and HER2 in the different cluster cell lines, and the protein expression normalised by the housekeeper GAPDH. Points represent mean measurements for three independent biological replicates with error bars representing the SEM.

Article Snippet: RT112, obtained from the German Collection of Microorganisms and Cell Cultures GmbH (DSMZ), and 5637 cells (kindly provided by Prof. Holm, Department of Oral and Maxillofacial Surgery, Medical University Innsbruck) were cultured in RPMI 1640 medium (PAN‐Biotech).

Techniques: In Vitro, RNA Sequencing, Control, Activity Assay, Western Blot, Expressing

Journal: Molecules

Article Title: Exploring EGFR, Nectin-4, and TROP-2 as Therapeutic Targets for Bladder Cancer Photoimmunotherapy

doi: 10.3390/molecules30244802

Figure Lengend Snippet: ( a ) Western blot analysis of EGFR, Nectin-4, and TROP-2 expression in the BC cell lines RT4 and RT112 and in the antigen-negative control cell line CHO. ( b ) Cell binding of the antibodies and conjugates to the target cells as demonstrated by flow cytometry. Binding of the isotype control antibody is shown in gray.

Article Snippet: RT4 cells were cultivated in EMEM medium (Cytion, Eppelheim, Germany), RT112 cells in RPMI1640 medium (Gibco), and CHO cells in F-12 Nutrient Mixture Medium (Gibco) at 37 °C and 5% CO 2 .

Techniques: Western Blot, Expressing, Negative Control, Binding Assay, Flow Cytometry, Control

Journal: Molecules

Article Title: Exploring EGFR, Nectin-4, and TROP-2 as Therapeutic Targets for Bladder Cancer Photoimmunotherapy

doi: 10.3390/molecules30244802

Figure Lengend Snippet: Cytotoxicity of the conjugates Cmb T120C/D265C -WB692-CB2, Enf T120C/D265C -WB692-CB2, and Sac T120C/D265C -WB692-CB2 after irradiation with different light doses in RT4 and RT112 cells. CHO cells served as antigen-negative controls. Mean values ± SD of three independent biological experiments. Statistical analyses were performed using unpaired, parametric Student’s t -tests with Welch’s correction (* p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0.001).

Article Snippet: RT4 cells were cultivated in EMEM medium (Cytion, Eppelheim, Germany), RT112 cells in RPMI1640 medium (Gibco), and CHO cells in F-12 Nutrient Mixture Medium (Gibco) at 37 °C and 5% CO 2 .

Techniques: Irradiation

Journal: Molecules

Article Title: Exploring EGFR, Nectin-4, and TROP-2 as Therapeutic Targets for Bladder Cancer Photoimmunotherapy

doi: 10.3390/molecules30244802

Figure Lengend Snippet: Cytotoxicity of the conjugates Cmb T120C/D265C -WB692-CB2, Enf T120C/D265C -WB692-CB2 and Sac 120T/265C -WB692-CB2 alone and in combination after irradiation with a light dose of 64 J/cm 2 in RT4 and RT112 cells. Control samples were treated with the uncoupled antibodies or the free dye. Mean values ± SD of three independent biological experiments. Statistical analyses were performed using unpaired, parametric Student’s t -tests with Welch’s correction (* p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0.001).

Article Snippet: RT4 cells were cultivated in EMEM medium (Cytion, Eppelheim, Germany), RT112 cells in RPMI1640 medium (Gibco), and CHO cells in F-12 Nutrient Mixture Medium (Gibco) at 37 °C and 5% CO 2 .

Techniques: Irradiation, Control