reverse transcription enzyme (Thermo Fisher)
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Thermo Fisher
reverse transcription enzyme
Reverse Transcription Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reverse+transcript+enzyme/maxima+h+minus+reverse+transcriptase/pmc11564529-242-40-43
Average 90 stars, based on 1 article reviews
Reverse Transcription Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reverse+transcript+enzyme/maxima+h+minus+reverse+transcriptase/pmc11564529-242-40-43
Average 90 stars, based on 1 article reviews
reverse transcription enzyme - by Bioz Stars,
2026-09
90/100 stars
Images
1) Product Images from "Dyna-vivo-seq unveils cellular RNA dynamics during acute kidney injury via in vivo metabolic RNA labeling-based scRNA-seq"
Article Title: Dyna-vivo-seq unveils cellular RNA dynamics during acute kidney injury via in vivo metabolic RNA labeling-based scRNA-seq
Journal: Nature Communications
doi: 10.1038/s41467-024-54202-4
Figure Legend Snippet: a UMAP showing PST cells from distinct time points. b UMAP showing the fraction of labeled transcripts per cell in vivo 4sU-labeled PST cells. c UMAP showing H-PST and L-PST cell clusters. UMAP visualization of H-PST cells responded to uni-IRI for distinct time points characterized by conventional splicing-based ( d ) or metabolic labeling-based RNA velocity analysis ( e ). Cells are color-coded by treatment time. The streamlines reveal the integration paths of local projections moving from the observed state to the extrapolated future state. f UMAP visualization of L-PST cells responded to uni-IRI for distinct time points characterized by metabolic labeling-based RNA velocity analysis. g Bar plot showing the expression of Havcr1 in H-PST and L-PST cells at distinct time points. n = 2240 cells for L-PST, n = 973 for H-PST. Data shown as mean ± SD. h Phase portraits showing total-new RNA planes of Havcr1 . i UMAP plots colored by smoothed total RNA level based on local averaging. The regulon activity of transcription factors Jun ( j ) and Cebpb ( k ) along with the AKI progression. In new RNA-based analysis, n = 114, 870, 79, 241, and 251 cells for NC, I_30 min, I_50 min, IR_3.8 h, and IR_5.3 h groups; In old RNA-based analysis, n = 54, 708, 44, 19, and 32 cells for NC, I_30 min, I_50 min, IR_3.8 h, and IR_5.3 h groups. Data shown as mean ± SD. l Signaling pathway role heatmap showing THBS signaling pathway network in Fib, C1q- Macro, C1q+ Macro, and H-PST cells. Source data are provided as a Source Data file.
Techniques Used: Labeling, In Vivo, Expressing, Activity Assay
Related Articles
Reverse Transcription:Article Title: Optogenetic Clustering of Human IRE1 Reveals Differential Regulation of Transcription and mRNA Splice Isoform Abundance by the UPR Article Snippet: .. The reaction was then quickly mixed with 0.5 μl RNaseOUT (Thermo Fisher #10777019), 1 μl of 20mM strand-switching primer, 1 μl Article Title: Deciphering direct transcriptional effects of epigenetic compounds through large-scale new RNA profiling Article Snippet: Briefly, 2 μl purified RNA from each sample was transferred to a 384 well plate and 2 μl alkylation mix (50 mM Tris-HCl pH 8.4, 45% DMSO (Sigma), 200 mM iodoacetamide (Sigma)) was added to each well and the plate was incubated at 50 °C for 15 min. To quench the alkylation reaction, 2 μl quenching mix (35 mM DTT (Thermo Scientific), 0.5 mM dNTPs (Thermo Scientific), 0.5 μM Smart-seq3 oligo-dT primer (5′-biotin-ACGAGCATCAGCAGCATACGA T30VN-3′; IDT), 0.4 U/μl RRI (Takara)) was added and the plate was incubated for 5 min at 21 °C, then 10 min at 72 °C to denature any RNA secondary structures. .. Reverse transcription master mix (25 mM Tris-HCl pH 8.0, 35 mM NaCl (Sigma), 1 mM GTP (Thermo Scientific), 3.6 mM MgCl 2 (invitrogen), 5% PEG (Sigma), 2 mM DTT (Thermo Scientific), 0,4 U/μl RNase Inhibitor (Takara), Smart-seq3 TSO oligo (5′-biotin-AGAGACAGATTGCGCAATGNNNNNNNNrGrGrG-3′; IDT), 2 U/μl Article Title: Nucleosome Positioning Shapes Cryptic Antisense Transcription Article Snippet: .. Reverse transcription was then performed using Article Title: The rewiring of cAMP/cGMP and LDH signalling drives cardiac hypertrophy in Pde5a -/- mice. Article Snippet: The yield and purity of RNA was determined with the NanoDrop OneC microvolume UV-Vis Spectrophotometer (Thermo Fisher Scientific). .. RNA was reverse transcribed by Article Title: Genetically encoded and modular subcellular organelle probes reveal dysfunction in lysosomes and mitochondria driven by PRKN knockout Article Snippet: RNA extraction kit , Zymo , Cat# R2062. .. Article Title: Genetically encoded and modular subcellular organelle probes reveal dysfunction in lysosomes and mitochondria driven by PRKN knockout Article Snippet: RNA was extracted from samples using TRIzol (Invitrogen 15596018) and an RNA extraction kit following the manufacturer’s recommended protocol (Zymo R2062). .. RNA concentration and purity were measured on a spectrophotometer. cDNA was produced from 1 μg of RNA per sample, using Article Title: Nanoscale 3D DNA tracing in non-denatured cells resolves the Cohesin-dependent loop architecture of the genome in situ Article Snippet: .. At this point, 1 μl of this sample was set-aside for quality control. ssDNA amplification from template RNA was performed by reverse transcription, using: 150 μl of unpurified in vitro transcription reaction, 1.7 mM dNTP mix, 19 μM forward primer, 240 U RNAsin Plus RNAse inhibitor (Promega), 1200 U Incubation:Article Title: Deciphering direct transcriptional effects of epigenetic compounds through large-scale new RNA profiling Article Snippet: Briefly, 2 μl purified RNA from each sample was transferred to a 384 well plate and 2 μl alkylation mix (50 mM Tris-HCl pH 8.4, 45% DMSO (Sigma), 200 mM iodoacetamide (Sigma)) was added to each well and the plate was incubated at 50 °C for 15 min. To quench the alkylation reaction, 2 μl quenching mix (35 mM DTT (Thermo Scientific), 0.5 mM dNTPs (Thermo Scientific), 0.5 μM Smart-seq3 oligo-dT primer (5′-biotin-ACGAGCATCAGCAGCATACGA T30VN-3′; IDT), 0.4 U/μl RRI (Takara)) was added and the plate was incubated for 5 min at 21 °C, then 10 min at 72 °C to denature any RNA secondary structures. .. Reverse transcription master mix (25 mM Tris-HCl pH 8.0, 35 mM NaCl (Sigma), 1 mM GTP (Thermo Scientific), 3.6 mM MgCl 2 (invitrogen), 5% PEG (Sigma), 2 mM DTT (Thermo Scientific), 0,4 U/μl RNase Inhibitor (Takara), Smart-seq3 TSO oligo (5′-biotin-AGAGACAGATTGCGCAATGNNNNNNNNrGrGrG-3′; IDT), 2 U/μl Polymerase Chain Reaction:Article Title: Nucleosome Positioning Shapes Cryptic Antisense Transcription Article Snippet: .. Reverse transcription was then performed using other:Article Title: Deciphering direct transcriptional effects of epigenetic compounds through large-scale new RNA profiling Article Snippet: Following the quenching reaction, the cells were denatured at 72 °C for 10 min and 3 μl RT reaction (2 μl TSO (5’-Biotin-AGAGACAGATTGCGCAATGNNNNNNNNrGrGrG-3’; IDT), 25 mM Tris-HCl (pH 8.0), 35 mM NaCl, 1 mM Real-time Polymerase Chain Reaction:Article Title: The rewiring of cAMP/cGMP and LDH signalling drives cardiac hypertrophy in Pde5a -/- mice. Article Snippet: The yield and purity of RNA was determined with the NanoDrop OneC microvolume UV-Vis Spectrophotometer (Thermo Fisher Scientific). .. RNA was reverse transcribed by Article Title: Genetically encoded and modular subcellular organelle probes reveal dysfunction in lysosomes and mitochondria driven by PRKN knockout Article Snippet: RNA was extracted from samples using TRIzol (Invitrogen 15596018) and an RNA extraction kit following the manufacturer’s recommended protocol (Zymo R2062). .. RNA concentration and purity were measured on a spectrophotometer. cDNA was produced from 1 μg of RNA per sample, using SYBR Green Assay:Article Title: The rewiring of cAMP/cGMP and LDH signalling drives cardiac hypertrophy in Pde5a -/- mice. Article Snippet: The yield and purity of RNA was determined with the NanoDrop OneC microvolume UV-Vis Spectrophotometer (Thermo Fisher Scientific). .. RNA was reverse transcribed by Article Title: Genetically encoded and modular subcellular organelle probes reveal dysfunction in lysosomes and mitochondria driven by PRKN knockout Article Snippet: RNA was extracted from samples using TRIzol (Invitrogen 15596018) and an RNA extraction kit following the manufacturer’s recommended protocol (Zymo R2062). .. RNA concentration and purity were measured on a spectrophotometer. cDNA was produced from 1 μg of RNA per sample, using Concentration Assay:Article Title: Genetically encoded and modular subcellular organelle probes reveal dysfunction in lysosomes and mitochondria driven by PRKN knockout Article Snippet: RNA was extracted from samples using TRIzol (Invitrogen 15596018) and an RNA extraction kit following the manufacturer’s recommended protocol (Zymo R2062). .. RNA concentration and purity were measured on a spectrophotometer. cDNA was produced from 1 μg of RNA per sample, using Spectrophotometry:Article Title: Genetically encoded and modular subcellular organelle probes reveal dysfunction in lysosomes and mitochondria driven by PRKN knockout Article Snippet: RNA was extracted from samples using TRIzol (Invitrogen 15596018) and an RNA extraction kit following the manufacturer’s recommended protocol (Zymo R2062). .. RNA concentration and purity were measured on a spectrophotometer. cDNA was produced from 1 μg of RNA per sample, using Produced:Article Title: Genetically encoded and modular subcellular organelle probes reveal dysfunction in lysosomes and mitochondria driven by PRKN knockout Article Snippet: RNA was extracted from samples using TRIzol (Invitrogen 15596018) and an RNA extraction kit following the manufacturer’s recommended protocol (Zymo R2062). .. RNA concentration and purity were measured on a spectrophotometer. cDNA was produced from 1 μg of RNA per sample, using Control:Article Title: Nanoscale 3D DNA tracing in non-denatured cells resolves the Cohesin-dependent loop architecture of the genome in situ Article Snippet: .. At this point, 1 μl of this sample was set-aside for quality control. ssDNA amplification from template RNA was performed by reverse transcription, using: 150 μl of unpurified in vitro transcription reaction, 1.7 mM dNTP mix, 19 μM forward primer, 240 U RNAsin Plus RNAse inhibitor (Promega), 1200 U Amplification:Article Title: Nanoscale 3D DNA tracing in non-denatured cells resolves the Cohesin-dependent loop architecture of the genome in situ Article Snippet: .. At this point, 1 μl of this sample was set-aside for quality control. ssDNA amplification from template RNA was performed by reverse transcription, using: 150 μl of unpurified in vitro transcription reaction, 1.7 mM dNTP mix, 19 μM forward primer, 240 U RNAsin Plus RNAse inhibitor (Promega), 1200 U In Vitro:Article Title: Nanoscale 3D DNA tracing in non-denatured cells resolves the Cohesin-dependent loop architecture of the genome in situ Article Snippet: .. At this point, 1 μl of this sample was set-aside for quality control. ssDNA amplification from template RNA was performed by reverse transcription, using: 150 μl of unpurified in vitro transcription reaction, 1.7 mM dNTP mix, 19 μM forward primer, 240 U RNAsin Plus RNAse inhibitor (Promega), 1200 U |