Review



the reverse transcription enzyme  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90

    Structured Review

    Thermo Fisher the reverse transcription enzyme
    The Reverse Transcription Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reverse+transcript+enzyme/pmc11619524-209-1-10
    Average 90 stars, based on 1 article reviews
    the reverse transcription enzyme - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    other:

    Article Title: Protective effects of methylnissolin and methylnissolin-3-O-β-d-glucopyranoside on TNF-α-induced inflammation in human dermal fibroblasts.
    Article Snippet: Methylnissolin-3-O-β-D-glucopyranoside (MNG) and methylnissolin (MN) are pterocarpan derivatives that are found in plants, such as Astragalus membranaceus.. There are limited existing studies on the potential health benefits of MNG, and currently there is no evidence to suggest that MNG has any impact on skin-aging.. Tumor necrosis factor-alpha (TNF-α) plays a significant role in skin aging by promoting chronic inflammation, damaging skin cells, and impairing the skin’s natural repair mechanisms.

    Article Title: Markers of mitochondrial function and oxidative metabolism in female skeletal muscle do not display intrinsic circadian regulation
    Article Snippet: RNA was extracted with TRIzol (Invitrogen) and RNA concentration and quality was measured (NanoDrop 2000 spectrophotometer), with 500ng of RNA used for cDNA synthesis (Applied Biosystems).

    Article Title: Pseudomonas plecoglossicida infection induces neutrophil autophagy-driven NETosis in large yellow croaker Larimichthys crocea
    Article Snippet: The quantified RNA samples were then used for cDNA synthesis (Invitrogen, Carlsbad, CA, USA).

    Article Title: Complete primer set for amplification and expression of full-length recombinant human monoclonal antibodies from single human B cells.
    Article Snippet: Human monoclonal antibodies (mAbs) are an important segment in precision therapeutics.. Various methodologies are available for generating them.. Recombinant human mAbs expression from sorted single B cells is preferred for its rapid expression using mammalian vectors while maintaining in vivo immunoglobulin (Ig) pairing.

    Article Title: Mitochondrial ATP-Sensitive K + Channels (MitoKATP) Regulate Brown Adipocyte Differentiation and Metabolism
    Article Snippet: 0.6 to 1 μg of RNA were employed for cDNA synthesis (High-Capacity System, Life Technologies, USA).

    Cell Culture:

    Article Title: PLGA-Nano-Encapsulated Disulfiram Inhibits Cancer Stem Cells and Targets Non-Small Cell Lung Cancer In Vitro and In Vivo
    Article Snippet: .. RNA was extracted from cultured NSCLC cells using an RNA purification kit (Norgen Biotek, Thorold, ON, Canada)) following the manufacturer’s instructions, as a template for cDNA synthesis using Multiscribe Reverse Transcriptase (Applied Biosystems, Woburn, MA, USA). .. The following primers/probes were used: TaqmanTM gene expression assay ID P65 Hs01042014_m1, HIF1A Hs00936371_m1, HIF2A Hs01026149_m1, SOX2 Hs01053049_s1, OCT4 Hs00999632_g1, NANOG Hs02387400_g1, HPRT1 Hs99999909_m1.

    Purification:

    Article Title: PLGA-Nano-Encapsulated Disulfiram Inhibits Cancer Stem Cells and Targets Non-Small Cell Lung Cancer In Vitro and In Vivo
    Article Snippet: .. RNA was extracted from cultured NSCLC cells using an RNA purification kit (Norgen Biotek, Thorold, ON, Canada)) following the manufacturer’s instructions, as a template for cDNA synthesis using Multiscribe Reverse Transcriptase (Applied Biosystems, Woburn, MA, USA). .. The following primers/probes were used: TaqmanTM gene expression assay ID P65 Hs01042014_m1, HIF1A Hs00936371_m1, HIF2A Hs01026149_m1, SOX2 Hs01053049_s1, OCT4 Hs00999632_g1, NANOG Hs02387400_g1, HPRT1 Hs99999909_m1.

    cDNA Synthesis:

    Article Title: PLGA-Nano-Encapsulated Disulfiram Inhibits Cancer Stem Cells and Targets Non-Small Cell Lung Cancer In Vitro and In Vivo
    Article Snippet: .. RNA was extracted from cultured NSCLC cells using an RNA purification kit (Norgen Biotek, Thorold, ON, Canada)) following the manufacturer’s instructions, as a template for cDNA synthesis using Multiscribe Reverse Transcriptase (Applied Biosystems, Woburn, MA, USA). .. The following primers/probes were used: TaqmanTM gene expression assay ID P65 Hs01042014_m1, HIF1A Hs00936371_m1, HIF2A Hs01026149_m1, SOX2 Hs01053049_s1, OCT4 Hs00999632_g1, NANOG Hs02387400_g1, HPRT1 Hs99999909_m1.

    Article Title: Exploring mosquito virome dynamics within São Paulo Zoo: insights into mosquito-virus-environment interactions
    Article Snippet: The filtrate was subjected to treatment with a mixture of nuclease enzymes (7 μL of TURBO DNase and 3 μL RNase Cocktail Enzyme Mix, Thermo Fisher Scientific, Waltham, MA, USA) in order to digest unprotected nucleic acids. .. RNA extraction was performed with Maxwell 16 Viral Total Nucleic Acid Purification kit (Promega, Inc., Madison, WI, USA) according to the manufacturer’s instructions and cDNA synthesis was performed with SuperScript IV (Thermo Fisher Scientific, Waltham, MA, USA). .. A second strand of cDNA synthesis was performed using DNA Polymerase I Large (Klenow) Fragment (Promega Inc., Madison, WI, USA).

    Article Title: The role of morphological adaptability in Vibrio cholerae ’s motility
    Article Snippet: Luminescence was measured using a GloMax 20/20 luminometer (Promega). .. Briefly, induction of filamentous V. cholerae was performed, followed by incubation at the specified time points, samples underwent centrifugation and were resuspended in 1 mL TRIzol (Invitrogen, MA, USA) for RNA extraction, utilizing the Qiagen RNeasy Kit for extraction and the TURBO DNA-free Kit (Ambion, TX, USA) for DNA digestion, in accordance with the manufacturers’ instructions. cDNA synthesis was conducted using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, MA, USA), and qPCR analysis was carried out on two 384-well plates employing PowerUp SYBR Green Master Mix (Applied Biosystems) on QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems). ..

    Reverse Transcription:

    Article Title: PLGA-Nano-Encapsulated Disulfiram Inhibits Cancer Stem Cells and Targets Non-Small Cell Lung Cancer In Vitro and In Vivo
    Article Snippet: .. RNA was extracted from cultured NSCLC cells using an RNA purification kit (Norgen Biotek, Thorold, ON, Canada)) following the manufacturer’s instructions, as a template for cDNA synthesis using Multiscribe Reverse Transcriptase (Applied Biosystems, Woburn, MA, USA). .. The following primers/probes were used: TaqmanTM gene expression assay ID P65 Hs01042014_m1, HIF1A Hs00936371_m1, HIF2A Hs01026149_m1, SOX2 Hs01053049_s1, OCT4 Hs00999632_g1, NANOG Hs02387400_g1, HPRT1 Hs99999909_m1.

    Article Title: The role of morphological adaptability in Vibrio cholerae ’s motility
    Article Snippet: Luminescence was measured using a GloMax 20/20 luminometer (Promega). .. Briefly, induction of filamentous V. cholerae was performed, followed by incubation at the specified time points, samples underwent centrifugation and were resuspended in 1 mL TRIzol (Invitrogen, MA, USA) for RNA extraction, utilizing the Qiagen RNeasy Kit for extraction and the TURBO DNA-free Kit (Ambion, TX, USA) for DNA digestion, in accordance with the manufacturers’ instructions. cDNA synthesis was conducted using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, MA, USA), and qPCR analysis was carried out on two 384-well plates employing PowerUp SYBR Green Master Mix (Applied Biosystems) on QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems). ..

    RNA Extraction:

    Article Title: Exploring mosquito virome dynamics within São Paulo Zoo: insights into mosquito-virus-environment interactions
    Article Snippet: The filtrate was subjected to treatment with a mixture of nuclease enzymes (7 μL of TURBO DNase and 3 μL RNase Cocktail Enzyme Mix, Thermo Fisher Scientific, Waltham, MA, USA) in order to digest unprotected nucleic acids. .. RNA extraction was performed with Maxwell 16 Viral Total Nucleic Acid Purification kit (Promega, Inc., Madison, WI, USA) according to the manufacturer’s instructions and cDNA synthesis was performed with SuperScript IV (Thermo Fisher Scientific, Waltham, MA, USA). .. A second strand of cDNA synthesis was performed using DNA Polymerase I Large (Klenow) Fragment (Promega Inc., Madison, WI, USA).

    Article Title: The role of morphological adaptability in Vibrio cholerae ’s motility
    Article Snippet: Luminescence was measured using a GloMax 20/20 luminometer (Promega). .. Briefly, induction of filamentous V. cholerae was performed, followed by incubation at the specified time points, samples underwent centrifugation and were resuspended in 1 mL TRIzol (Invitrogen, MA, USA) for RNA extraction, utilizing the Qiagen RNeasy Kit for extraction and the TURBO DNA-free Kit (Ambion, TX, USA) for DNA digestion, in accordance with the manufacturers’ instructions. cDNA synthesis was conducted using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, MA, USA), and qPCR analysis was carried out on two 384-well plates employing PowerUp SYBR Green Master Mix (Applied Biosystems) on QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems). ..

    Nucleic Acid Purification:

    Article Title: Exploring mosquito virome dynamics within São Paulo Zoo: insights into mosquito-virus-environment interactions
    Article Snippet: The filtrate was subjected to treatment with a mixture of nuclease enzymes (7 μL of TURBO DNase and 3 μL RNase Cocktail Enzyme Mix, Thermo Fisher Scientific, Waltham, MA, USA) in order to digest unprotected nucleic acids. .. RNA extraction was performed with Maxwell 16 Viral Total Nucleic Acid Purification kit (Promega, Inc., Madison, WI, USA) according to the manufacturer’s instructions and cDNA synthesis was performed with SuperScript IV (Thermo Fisher Scientific, Waltham, MA, USA). .. A second strand of cDNA synthesis was performed using DNA Polymerase I Large (Klenow) Fragment (Promega Inc., Madison, WI, USA).

    Incubation:

    Article Title: The role of morphological adaptability in Vibrio cholerae ’s motility
    Article Snippet: Luminescence was measured using a GloMax 20/20 luminometer (Promega). .. Briefly, induction of filamentous V. cholerae was performed, followed by incubation at the specified time points, samples underwent centrifugation and were resuspended in 1 mL TRIzol (Invitrogen, MA, USA) for RNA extraction, utilizing the Qiagen RNeasy Kit for extraction and the TURBO DNA-free Kit (Ambion, TX, USA) for DNA digestion, in accordance with the manufacturers’ instructions. cDNA synthesis was conducted using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, MA, USA), and qPCR analysis was carried out on two 384-well plates employing PowerUp SYBR Green Master Mix (Applied Biosystems) on QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems). ..

    Centrifugation:

    Article Title: The role of morphological adaptability in Vibrio cholerae ’s motility
    Article Snippet: Luminescence was measured using a GloMax 20/20 luminometer (Promega). .. Briefly, induction of filamentous V. cholerae was performed, followed by incubation at the specified time points, samples underwent centrifugation and were resuspended in 1 mL TRIzol (Invitrogen, MA, USA) for RNA extraction, utilizing the Qiagen RNeasy Kit for extraction and the TURBO DNA-free Kit (Ambion, TX, USA) for DNA digestion, in accordance with the manufacturers’ instructions. cDNA synthesis was conducted using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, MA, USA), and qPCR analysis was carried out on two 384-well plates employing PowerUp SYBR Green Master Mix (Applied Biosystems) on QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems). ..

    Extraction:

    Article Title: The role of morphological adaptability in Vibrio cholerae ’s motility
    Article Snippet: Luminescence was measured using a GloMax 20/20 luminometer (Promega). .. Briefly, induction of filamentous V. cholerae was performed, followed by incubation at the specified time points, samples underwent centrifugation and were resuspended in 1 mL TRIzol (Invitrogen, MA, USA) for RNA extraction, utilizing the Qiagen RNeasy Kit for extraction and the TURBO DNA-free Kit (Ambion, TX, USA) for DNA digestion, in accordance with the manufacturers’ instructions. cDNA synthesis was conducted using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, MA, USA), and qPCR analysis was carried out on two 384-well plates employing PowerUp SYBR Green Master Mix (Applied Biosystems) on QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems). ..

    Real-time Polymerase Chain Reaction:

    Article Title: The role of morphological adaptability in Vibrio cholerae ’s motility
    Article Snippet: Luminescence was measured using a GloMax 20/20 luminometer (Promega). .. Briefly, induction of filamentous V. cholerae was performed, followed by incubation at the specified time points, samples underwent centrifugation and were resuspended in 1 mL TRIzol (Invitrogen, MA, USA) for RNA extraction, utilizing the Qiagen RNeasy Kit for extraction and the TURBO DNA-free Kit (Ambion, TX, USA) for DNA digestion, in accordance with the manufacturers’ instructions. cDNA synthesis was conducted using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, MA, USA), and qPCR analysis was carried out on two 384-well plates employing PowerUp SYBR Green Master Mix (Applied Biosystems) on QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems). ..

    SYBR Green Assay:

    Article Title: The role of morphological adaptability in Vibrio cholerae ’s motility
    Article Snippet: Luminescence was measured using a GloMax 20/20 luminometer (Promega). .. Briefly, induction of filamentous V. cholerae was performed, followed by incubation at the specified time points, samples underwent centrifugation and were resuspended in 1 mL TRIzol (Invitrogen, MA, USA) for RNA extraction, utilizing the Qiagen RNeasy Kit for extraction and the TURBO DNA-free Kit (Ambion, TX, USA) for DNA digestion, in accordance with the manufacturers’ instructions. cDNA synthesis was conducted using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, MA, USA), and qPCR analysis was carried out on two 384-well plates employing PowerUp SYBR Green Master Mix (Applied Biosystems) on QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems). ..



    Similar Products

    90
    Toyobo reverse transcript enzyme
    Reverse Transcript Enzyme, supplied by Toyobo, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reverse+transcript+enzyme/reverse+transcript+enzyme/pm40223164-87-1-4
    Average 90 stars, based on 1 article reviews
    reverse transcript enzyme - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Yeasen Biotechnology reverse transcription enzyme
    Reverse Transcription Enzyme, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reverse+transcript+enzyme/reverse+transcription+reagents/pmc10086289__gutjnl___2022___328035supp001-50-48-51
    Average 90 stars, based on 1 article reviews
    reverse transcription enzyme - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Zeesan Biotech Co Ltd lf enzyme 03 reverse transcription kit
    Lf Enzyme 03 Reverse Transcription Kit, supplied by Zeesan Biotech Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reverse+transcript+enzyme/lf+enzyme+03+reverse+transcription+kit/pmc12056533-54-5-12
    Average 90 stars, based on 1 article reviews
    lf enzyme 03 reverse transcription kit - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Promega enzymes for reverse transcription
    Enzymes For Reverse Transcription, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reverse+transcript+enzyme/reverse+transcription+kit/pmc12004191-180-8-18
    Average 90 stars, based on 1 article reviews
    enzymes for reverse transcription - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Fisher Scientific enzymes and reagents for the reverse transcription quantitative polymerase chain reactions (rt-qpcrs)
    Enzymes And Reagents For The Reverse Transcription Quantitative Polymerase Chain Reactions (Rt Qpcrs), supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reverse+transcript+enzyme/enzymes+and+reagents+for+the+reverse+transcription+quantitative+polymerase+chain+reactions++rt+qpcrs+/10__1016_slash_j__pestbp__2025__106391-61-6-15
    Average 90 stars, based on 1 article reviews
    enzymes and reagents for the reverse transcription quantitative polymerase chain reactions (rt-qpcrs) - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    96
    New England Biolabs reverse transcription
    Reverse Transcription, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reverse+transcript+enzyme/Template+Switching+RT+Enzyme+Mix/bio_rxiv__2025__02__07__637038-207-11-21
    Average 96 stars, based on 1 article reviews
    reverse transcription - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    99
    TaKaRa reverse transcription enzyme
    Reverse Transcription Enzyme, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reverse+transcript+enzyme/PrimeScript+RT+Master+Mix/pm39742725-105-1-5
    Average 99 stars, based on 1 article reviews
    reverse transcription enzyme - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    90
    Thermo Fisher superscript iv vilo master mix with ezdnase enzyme reverse transcription kit
    Superscript Iv Vilo Master Mix With Ezdnase Enzyme Reverse Transcription Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reverse+transcript+enzyme/superscript+iv+vilo+master+mix+with+ezdnase+enzyme/pm38500596-101-12-17
    Average 90 stars, based on 1 article reviews
    superscript iv vilo master mix with ezdnase enzyme reverse transcription kit - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher the reverse transcription enzyme
    The Reverse Transcription Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reverse+transcript+enzyme/pmc11619524-209-1-10
    Average 90 stars, based on 1 article reviews
    the reverse transcription enzyme - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher reverse transcription enzyme
    a UMAP showing PST cells from distinct time points. b UMAP showing the fraction of labeled transcripts per cell in vivo 4sU-labeled PST cells. c UMAP showing H-PST and L-PST cell clusters. UMAP visualization of H-PST cells responded to uni-IRI for distinct time points characterized by conventional splicing-based ( d ) or metabolic labeling-based RNA velocity analysis ( e ). Cells are color-coded by treatment time. The streamlines reveal the integration paths of local projections moving from the observed state to the extrapolated future state. f UMAP visualization of L-PST cells responded to uni-IRI for distinct time points characterized by metabolic labeling-based RNA velocity analysis. g Bar plot showing the expression of Havcr1 in H-PST and L-PST cells at distinct time points. n = 2240 cells for L-PST, n = 973 for H-PST. Data shown as mean ± SD. h Phase portraits showing total-new RNA planes of Havcr1 . i UMAP plots colored by smoothed total RNA level based on local averaging. The regulon activity of <t>transcription</t> factors Jun ( j ) and Cebpb ( k ) along with the AKI progression. In new RNA-based analysis, n = 114, 870, 79, 241, and 251 cells for NC, I_30 min, I_50 min, IR_3.8 h, and IR_5.3 h groups; In old RNA-based analysis, n = 54, 708, 44, 19, and 32 cells for NC, I_30 min, I_50 min, IR_3.8 h, and IR_5.3 h groups. Data shown as mean ± SD. l Signaling pathway role heatmap showing THBS signaling pathway network in Fib, C1q- Macro, C1q+ Macro, and H-PST cells. Source data are provided as a Source Data file.
    Reverse Transcription Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reverse+transcript+enzyme/maxima+h+minus+reverse+transcriptase/pmc11564529-242-40-43
    Average 90 stars, based on 1 article reviews
    reverse transcription enzyme - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    Image Search Results


    a UMAP showing PST cells from distinct time points. b UMAP showing the fraction of labeled transcripts per cell in vivo 4sU-labeled PST cells. c UMAP showing H-PST and L-PST cell clusters. UMAP visualization of H-PST cells responded to uni-IRI for distinct time points characterized by conventional splicing-based ( d ) or metabolic labeling-based RNA velocity analysis ( e ). Cells are color-coded by treatment time. The streamlines reveal the integration paths of local projections moving from the observed state to the extrapolated future state. f UMAP visualization of L-PST cells responded to uni-IRI for distinct time points characterized by metabolic labeling-based RNA velocity analysis. g Bar plot showing the expression of Havcr1 in H-PST and L-PST cells at distinct time points. n = 2240 cells for L-PST, n = 973 for H-PST. Data shown as mean ± SD. h Phase portraits showing total-new RNA planes of Havcr1 . i UMAP plots colored by smoothed total RNA level based on local averaging. The regulon activity of transcription factors Jun ( j ) and Cebpb ( k ) along with the AKI progression. In new RNA-based analysis, n = 114, 870, 79, 241, and 251 cells for NC, I_30 min, I_50 min, IR_3.8 h, and IR_5.3 h groups; In old RNA-based analysis, n = 54, 708, 44, 19, and 32 cells for NC, I_30 min, I_50 min, IR_3.8 h, and IR_5.3 h groups. Data shown as mean ± SD. l Signaling pathway role heatmap showing THBS signaling pathway network in Fib, C1q- Macro, C1q+ Macro, and H-PST cells. Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Dyna-vivo-seq unveils cellular RNA dynamics during acute kidney injury via in vivo metabolic RNA labeling-based scRNA-seq

    doi: 10.1038/s41467-024-54202-4

    Figure Lengend Snippet: a UMAP showing PST cells from distinct time points. b UMAP showing the fraction of labeled transcripts per cell in vivo 4sU-labeled PST cells. c UMAP showing H-PST and L-PST cell clusters. UMAP visualization of H-PST cells responded to uni-IRI for distinct time points characterized by conventional splicing-based ( d ) or metabolic labeling-based RNA velocity analysis ( e ). Cells are color-coded by treatment time. The streamlines reveal the integration paths of local projections moving from the observed state to the extrapolated future state. f UMAP visualization of L-PST cells responded to uni-IRI for distinct time points characterized by metabolic labeling-based RNA velocity analysis. g Bar plot showing the expression of Havcr1 in H-PST and L-PST cells at distinct time points. n = 2240 cells for L-PST, n = 973 for H-PST. Data shown as mean ± SD. h Phase portraits showing total-new RNA planes of Havcr1 . i UMAP plots colored by smoothed total RNA level based on local averaging. The regulon activity of transcription factors Jun ( j ) and Cebpb ( k ) along with the AKI progression. In new RNA-based analysis, n = 114, 870, 79, 241, and 251 cells for NC, I_30 min, I_50 min, IR_3.8 h, and IR_5.3 h groups; In old RNA-based analysis, n = 54, 708, 44, 19, and 32 cells for NC, I_30 min, I_50 min, IR_3.8 h, and IR_5.3 h groups. Data shown as mean ± SD. l Signaling pathway role heatmap showing THBS signaling pathway network in Fib, C1q- Macro, C1q+ Macro, and H-PST cells. Source data are provided as a Source Data file.

    Article Snippet: The barcoded beads were then resuspended in a reverse transcription mix, consisting of 1× RT buffer, 1 mM dNTP (TransGen Biotech, cat# AD101-12), 2.5 μM Template Switch Oligo (Sangon, 5’-AAGCAGTGGTATCAACGCAGAGTGAATrGrGrG-3’), 1U/μL Recombination RNase Inhibitor (TaKaRa, cat# 2313 A), 10 U/μL reverse transcription enzyme (Thermo Fisher, cat# EP0753), 1 mM GTP (Thermo Fisher, cat# R0461), and 5% PEG-8000 (Beyotime, cat# R0056).

    Techniques: Labeling, In Vivo, Expressing, Activity Assay