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reverse transcription enzyme  (TaKaRa)


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    Structured Review

    TaKaRa reverse transcription enzyme
    Reverse Transcription Enzyme, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 26640 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reverse+transcript+enzyme/PrimeScript+RT+Master+Mix/pm39742725-105-1-5
    Average 99 stars, based on 26640 article reviews
    reverse transcription enzyme - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    cDNA Synthesis:

    Article Title: Hematopoietic progenitor kinase 1 inhibitor BGB-15025 induces apoptosis in acute myeloid leukemia cells through the cell cycle pathway and mitogen-activated protein kinase/extracellular signal-regulated kinase pathway signaling axis
    Article Snippet: Monocyte-derived total RNA was extracted using the TransZol Up Plus RNA kit (TransGen Biotech) following manufacturer-recommended protocols. .. First-strand cDNA synthesis was performed with the PrimeScript RT Master Mix (RR037Q; Takara Bio, Shiga, Japan) through reverse transcription of 1 μg total RNA. .. Quantitative PCR amplification was conducted in triplicate reactions (10 μl total volume containing 1 μl cDNA template) using TB Green Premix Ex Taq II (#639676; Takara Bio) on a 7500 Fast Real-Time PCR System (Applied Biosystems, Singapore, Singapore).

    Reverse Transcription:

    Article Title: Hematopoietic progenitor kinase 1 inhibitor BGB-15025 induces apoptosis in acute myeloid leukemia cells through the cell cycle pathway and mitogen-activated protein kinase/extracellular signal-regulated kinase pathway signaling axis
    Article Snippet: Monocyte-derived total RNA was extracted using the TransZol Up Plus RNA kit (TransGen Biotech) following manufacturer-recommended protocols. .. First-strand cDNA synthesis was performed with the PrimeScript RT Master Mix (RR037Q; Takara Bio, Shiga, Japan) through reverse transcription of 1 μg total RNA. .. Quantitative PCR amplification was conducted in triplicate reactions (10 μl total volume containing 1 μl cDNA template) using TB Green Premix Ex Taq II (#639676; Takara Bio) on a 7500 Fast Real-Time PCR System (Applied Biosystems, Singapore, Singapore).

    Article Title: Extracellular biogenic nanoscale mitochondria reprogram the wound microenvironment via ROS scavenging independent of cellular uptake
    Article Snippet: Total RNA was extracted from cultured cells and peri-wound tissue samples using the RNeasy Mini Kit (Qiagen, Cat# 74014), following the manufacturer's protocol. .. Reverse transcription was performed using the PrimeScriptTM RT Master Mix (Takara, Cat# RR036A). .. Quantitative real-time PCR (qPCR) was conducted using SYBR Green chemistry on an ABI 7500 Real-Time PCR System (Applied Biosystems).

    Article Title: Inhalable PD-L1-engineered hybrid cellular vesicles suppress excessive neutrophil activation and restore mitochondrial homeostasis to alleviate ischemia–reperfusion lung injury and pneumonia
    Article Snippet: .. Total RNA was extracted using an RNA extraction kit (RC112, Vazyme, China) and reverse-transcribed into cDNA with a PCR kit (RR036A, Takara, Japan). .. Amplification reactions were carried out using the SYBR Green method on a real-time PCR system (Bio-Rad, USA).

    Synthesized:

    Article Title: AdMSC spheroids encapsulating antioxidant hybrid protein carrier for irradiation-damaged salivary gland repair
    Article Snippet: Total RNA was isolated from SG tissue using TRIzolTM LS Reagent (Invitrogen, Cat. No. 10296010) according to the manufacturer's protocol. .. Complementary DNA (cDNA) was synthesized from 500 to 1000 ng of total RNA using PrimeScript RT Master Mix (TaKaRa, Cat. No. RR036A), and quantitative PCR was performed with TB Green® Premix Ex TaqTM (TaKaRa, Cat. No. RR420A) on a real-time PCR system using gene-specific primers for VEGF, HGF, CD31, and vWF, with GAPDH as the internal reference gene. ..

    Real-time Polymerase Chain Reaction:

    Article Title: AdMSC spheroids encapsulating antioxidant hybrid protein carrier for irradiation-damaged salivary gland repair
    Article Snippet: Total RNA was isolated from SG tissue using TRIzolTM LS Reagent (Invitrogen, Cat. No. 10296010) according to the manufacturer's protocol. .. Complementary DNA (cDNA) was synthesized from 500 to 1000 ng of total RNA using PrimeScript RT Master Mix (TaKaRa, Cat. No. RR036A), and quantitative PCR was performed with TB Green® Premix Ex TaqTM (TaKaRa, Cat. No. RR420A) on a real-time PCR system using gene-specific primers for VEGF, HGF, CD31, and vWF, with GAPDH as the internal reference gene. ..

    Article Title: Adaptable sliding hydrogels enable pericellular pocket formation while enhancing MSC chondrogenesis and survival in 3D
    Article Snippet: Hydrogels were transferred to TRIzol (Thermo Fisher Scientific), crushed, and homogenized for phenol-chloroform RNA extraction and reverse transcript PCR (RT-PCR). .. Following preparation of cDNA using Primescript RT Master Mix (Takara Bio) in a MiniAmp Plus Thermal Cycler, q-PCR was conducted using Power SYBR Green PCR Master Mix (Applied Biosciences) and an Applied Biosystems QuantStudio 6 Pro Real-Time PCR system. ..

    Article Title: Intelligent-responsive hydrogel synergistically mediates immune remodel-antibacterial-angiogenesis cascade for diabetic foot ulcer repair
    Article Snippet: .. Methyl alcohol, Dimethyl sulfoxide (DMSO; General-Reagent, Titan, Shanghai, China); Deferoxamine mesylate (DFO; MCE, New Jersey, USA); Zn(NO 3 ) 2 ·6H 2 O, Mn(NO 3 ) 2 ·4H 2 O, 2-methylimidazole (Aladdin, Shanghai, China); UCMSC-exo (Langfang Kangbao Huitai Biotechnology, Langfang, China); Chitosan (CS; Deacetylation degree ≥95%, Macklin, Shanghai, China), 4-carboxyphenylboronic acid; polyvinyl alcohol (4-CPBA, PVA; Aladdin, Shanghai, China); N-(3-Dimethylaminopropyl)-N′-ethyl carbodiimide hydrochloride (EDC; Aladdin, Shanghai, China); N-Hydroxysuccinimide (NHS; Aladdin, Shanghai, China); PrimeScript RT Master Mix (Takara, Beijing, China); qPCR SYBR Green Master Mix (Yeasen, Shanghai, China); CD9 antibody (Selleck, USA); TSG101 antibody (Abmart, Shanghai, China); CD86 flow antibody and CD206 flow antibody (BioLegend, USA); IL-6, CD206, HIF-1α, CK14, Ki67, CD31, VEGF and α-SMA (AiFang biological, China). ..

    RNA Extraction:

    Article Title: Inhalable PD-L1-engineered hybrid cellular vesicles suppress excessive neutrophil activation and restore mitochondrial homeostasis to alleviate ischemia–reperfusion lung injury and pneumonia
    Article Snippet: .. Total RNA was extracted using an RNA extraction kit (RC112, Vazyme, China) and reverse-transcribed into cDNA with a PCR kit (RR036A, Takara, Japan). .. Amplification reactions were carried out using the SYBR Green method on a real-time PCR system (Bio-Rad, USA).

    Polymerase Chain Reaction:

    Article Title: Inhalable PD-L1-engineered hybrid cellular vesicles suppress excessive neutrophil activation and restore mitochondrial homeostasis to alleviate ischemia–reperfusion lung injury and pneumonia
    Article Snippet: .. Total RNA was extracted using an RNA extraction kit (RC112, Vazyme, China) and reverse-transcribed into cDNA with a PCR kit (RR036A, Takara, Japan). .. Amplification reactions were carried out using the SYBR Green method on a real-time PCR system (Bio-Rad, USA).

    Article Title: Adaptable sliding hydrogels enable pericellular pocket formation while enhancing MSC chondrogenesis and survival in 3D
    Article Snippet: Hydrogels were transferred to TRIzol (Thermo Fisher Scientific), crushed, and homogenized for phenol-chloroform RNA extraction and reverse transcript PCR (RT-PCR). .. Following preparation of cDNA using Primescript RT Master Mix (Takara Bio) in a MiniAmp Plus Thermal Cycler, q-PCR was conducted using Power SYBR Green PCR Master Mix (Applied Biosciences) and an Applied Biosystems QuantStudio 6 Pro Real-Time PCR system. ..

    other:

    Article Title: Interplay between nuclear survivin and the PRC2 complex and its impact on H3K27me3-directed transcriptional repression
    Article Snippet: The samples were treated with DNase I, using the RNase free DNase kit (Qiagen1023460) and RNA concentration determined with a Nanodrop 2000 (Thermo Fisher Scientific).

    SYBR Green Assay:

    Article Title: Adaptable sliding hydrogels enable pericellular pocket formation while enhancing MSC chondrogenesis and survival in 3D
    Article Snippet: Hydrogels were transferred to TRIzol (Thermo Fisher Scientific), crushed, and homogenized for phenol-chloroform RNA extraction and reverse transcript PCR (RT-PCR). .. Following preparation of cDNA using Primescript RT Master Mix (Takara Bio) in a MiniAmp Plus Thermal Cycler, q-PCR was conducted using Power SYBR Green PCR Master Mix (Applied Biosciences) and an Applied Biosystems QuantStudio 6 Pro Real-Time PCR system. ..

    Article Title: Intelligent-responsive hydrogel synergistically mediates immune remodel-antibacterial-angiogenesis cascade for diabetic foot ulcer repair
    Article Snippet: .. Methyl alcohol, Dimethyl sulfoxide (DMSO; General-Reagent, Titan, Shanghai, China); Deferoxamine mesylate (DFO; MCE, New Jersey, USA); Zn(NO 3 ) 2 ·6H 2 O, Mn(NO 3 ) 2 ·4H 2 O, 2-methylimidazole (Aladdin, Shanghai, China); UCMSC-exo (Langfang Kangbao Huitai Biotechnology, Langfang, China); Chitosan (CS; Deacetylation degree ≥95%, Macklin, Shanghai, China), 4-carboxyphenylboronic acid; polyvinyl alcohol (4-CPBA, PVA; Aladdin, Shanghai, China); N-(3-Dimethylaminopropyl)-N′-ethyl carbodiimide hydrochloride (EDC; Aladdin, Shanghai, China); N-Hydroxysuccinimide (NHS; Aladdin, Shanghai, China); PrimeScript RT Master Mix (Takara, Beijing, China); qPCR SYBR Green Master Mix (Yeasen, Shanghai, China); CD9 antibody (Selleck, USA); TSG101 antibody (Abmart, Shanghai, China); CD86 flow antibody and CD206 flow antibody (BioLegend, USA); IL-6, CD206, HIF-1α, CK14, Ki67, CD31, VEGF and α-SMA (AiFang biological, China). ..



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    Thermo Fisher reverse transcription enzyme
    a UMAP showing PST cells from distinct time points. b UMAP showing the fraction of labeled transcripts per cell in vivo 4sU-labeled PST cells. c UMAP showing H-PST and L-PST cell clusters. UMAP visualization of H-PST cells responded to uni-IRI for distinct time points characterized by conventional splicing-based ( d ) or metabolic labeling-based RNA velocity analysis ( e ). Cells are color-coded by treatment time. The streamlines reveal the integration paths of local projections moving from the observed state to the extrapolated future state. f UMAP visualization of L-PST cells responded to uni-IRI for distinct time points characterized by metabolic labeling-based RNA velocity analysis. g Bar plot showing the expression of Havcr1 in H-PST and L-PST cells at distinct time points. n = 2240 cells for L-PST, n = 973 for H-PST. Data shown as mean ± SD. h Phase portraits showing total-new RNA planes of Havcr1 . i UMAP plots colored by smoothed total RNA level based on local averaging. The regulon activity of <t>transcription</t> factors Jun ( j ) and Cebpb ( k ) along with the AKI progression. In new RNA-based analysis, n = 114, 870, 79, 241, and 251 cells for NC, I_30 min, I_50 min, IR_3.8 h, and IR_5.3 h groups; In old RNA-based analysis, n = 54, 708, 44, 19, and 32 cells for NC, I_30 min, I_50 min, IR_3.8 h, and IR_5.3 h groups. Data shown as mean ± SD. l Signaling pathway role heatmap showing THBS signaling pathway network in Fib, C1q- Macro, C1q+ Macro, and H-PST cells. Source data are provided as a Source Data file.
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    Image Search Results


    a UMAP showing PST cells from distinct time points. b UMAP showing the fraction of labeled transcripts per cell in vivo 4sU-labeled PST cells. c UMAP showing H-PST and L-PST cell clusters. UMAP visualization of H-PST cells responded to uni-IRI for distinct time points characterized by conventional splicing-based ( d ) or metabolic labeling-based RNA velocity analysis ( e ). Cells are color-coded by treatment time. The streamlines reveal the integration paths of local projections moving from the observed state to the extrapolated future state. f UMAP visualization of L-PST cells responded to uni-IRI for distinct time points characterized by metabolic labeling-based RNA velocity analysis. g Bar plot showing the expression of Havcr1 in H-PST and L-PST cells at distinct time points. n = 2240 cells for L-PST, n = 973 for H-PST. Data shown as mean ± SD. h Phase portraits showing total-new RNA planes of Havcr1 . i UMAP plots colored by smoothed total RNA level based on local averaging. The regulon activity of transcription factors Jun ( j ) and Cebpb ( k ) along with the AKI progression. In new RNA-based analysis, n = 114, 870, 79, 241, and 251 cells for NC, I_30 min, I_50 min, IR_3.8 h, and IR_5.3 h groups; In old RNA-based analysis, n = 54, 708, 44, 19, and 32 cells for NC, I_30 min, I_50 min, IR_3.8 h, and IR_5.3 h groups. Data shown as mean ± SD. l Signaling pathway role heatmap showing THBS signaling pathway network in Fib, C1q- Macro, C1q+ Macro, and H-PST cells. Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Dyna-vivo-seq unveils cellular RNA dynamics during acute kidney injury via in vivo metabolic RNA labeling-based scRNA-seq

    doi: 10.1038/s41467-024-54202-4

    Figure Lengend Snippet: a UMAP showing PST cells from distinct time points. b UMAP showing the fraction of labeled transcripts per cell in vivo 4sU-labeled PST cells. c UMAP showing H-PST and L-PST cell clusters. UMAP visualization of H-PST cells responded to uni-IRI for distinct time points characterized by conventional splicing-based ( d ) or metabolic labeling-based RNA velocity analysis ( e ). Cells are color-coded by treatment time. The streamlines reveal the integration paths of local projections moving from the observed state to the extrapolated future state. f UMAP visualization of L-PST cells responded to uni-IRI for distinct time points characterized by metabolic labeling-based RNA velocity analysis. g Bar plot showing the expression of Havcr1 in H-PST and L-PST cells at distinct time points. n = 2240 cells for L-PST, n = 973 for H-PST. Data shown as mean ± SD. h Phase portraits showing total-new RNA planes of Havcr1 . i UMAP plots colored by smoothed total RNA level based on local averaging. The regulon activity of transcription factors Jun ( j ) and Cebpb ( k ) along with the AKI progression. In new RNA-based analysis, n = 114, 870, 79, 241, and 251 cells for NC, I_30 min, I_50 min, IR_3.8 h, and IR_5.3 h groups; In old RNA-based analysis, n = 54, 708, 44, 19, and 32 cells for NC, I_30 min, I_50 min, IR_3.8 h, and IR_5.3 h groups. Data shown as mean ± SD. l Signaling pathway role heatmap showing THBS signaling pathway network in Fib, C1q- Macro, C1q+ Macro, and H-PST cells. Source data are provided as a Source Data file.

    Article Snippet: The barcoded beads were then resuspended in a reverse transcription mix, consisting of 1× RT buffer, 1 mM dNTP (TransGen Biotech, cat# AD101-12), 2.5 μM Template Switch Oligo (Sangon, 5’-AAGCAGTGGTATCAACGCAGAGTGAATrGrGrG-3’), 1U/μL Recombination RNase Inhibitor (TaKaRa, cat# 2313 A), 10 U/μL reverse transcription enzyme (Thermo Fisher, cat# EP0753), 1 mM GTP (Thermo Fisher, cat# R0461), and 5% PEG-8000 (Beyotime, cat# R0056).

    Techniques: Labeling, In Vivo, Expressing, Activity Assay