gst raf rbd (Addgene inc)

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Addgene inc gst raf rbd
Gst Raf Rbd, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/raf1/Raf-1+GST+RBD+1-149+(Plasmid+%2313338)/pmc06128481-5-2-4
Average 92 stars, based on 20 article reviews

gst raf rbd - by Bioz Stars, 2026-10

92/100 stars

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Plasmid Preparation:

Article Title: KLHL7 promotes hepatocellular carcinoma progression and molecular therapy resistance by degrading RASA2.
Article Snippet: .. The prokaryotic plasmid expressing the GST-RAF1 RBD was purchased from Addgene (13338). ..

Article Title: KLHL7 promotes hepatocellular carcinoma progression and molecular therapy resistance by degrading RASA2
Article Snippet: pRK5-HA-Ubiquitin-K48 , addgene , Plasmid #17605. .. Raf-1 GST RBD 1-149 , addgene , Plasmid #13338. .. pLKO.1-blast , addgene , Plasmid #26655.

Article Title: KLHL7 promotes hepatocellular carcinoma progression and molecular therapy resistance by degrading RASA2.
Article Snippet: China Cat#CL-0120 HCCLM3 Qiao Xin Zhou Biotechnology Cat#ZQ0023 HEK293T Chinese Academy of Sciences Cat#SCSP-502 Experimental models: Organisms/strains BALB/c nude Mice Charles River Cat#194 Oligonucleotides shKLHL7(1): TCATCAGTGAAGCGCAGTATC This paper N/A shKLHL7(2): CTGCTAGAATTTCCGTGAATA This paper N/A shRASA2: GAATCTGCGCTACTATGTAGA This paper N/A ACTB Forward: GCTCCGGCATGTGCAAGG This paper N/A ACTB Reverse: GGCCTCGTCGCCCACATA This paper N/A (Continued on next page) iScience 26, 106914, June 16, 2023 15 .. KLHL7 Forward: CATCGTGTTGTTCTTGCTGC This paper N/A KLHL7 Reverse: AGCAAATTCCACCAGTTGTTC This paper N/A RASA2 Forward: GCTGTTTTGCCCCTTGTACG This paper N/A RASA2 Reverse: ATCCAGACATCGGGTAGCCA This paper N/A Recombinant DNA pcDNA3.1 (+) Invitrogen Cat#V79020 pLKO.1 puro addgene Plasmid #8453 pMD2.G addgene Plasmid #12259 psPAX2 addgene Plasmid #12260 pRK5-HA-Ubiquitin-WT addgene Plasmid #17608 pRK5-HA-Ubiquitin-K63 addgene Plasmid #17606 pRK5-HA-Ubiquitin-K48 addgene Plasmid #17605 Raf-1 GST RBD 1-149 addgene Plasmid #13338 pLKO.1-blast addgene Plasmid #26655 pLVX-IRES-Puro(Flag-SBP) This paper N/A pLV-Ubc-MCS-IRES-Bsd This paper N/A Software and algorithms Image-Pro Plus Media Cy https://www.mediacy.com/78-products/image-pro-plus Prism GraphPad https://www.graphpad.com/scientific-software/prism/ ..

Article Title: GLUT3 promotes macrophage signaling and function via RAS-mediated endocytosis in atopic dermatitis and wound healing
Article Snippet: .. Raf-1 GST RBD 1–149 was a gift from Channing Der (Addgene plasmid 13338) (65). ..

Expressing:

Bacteria:

Article Title: Baseline expression of c-Myc defines the tissue specificity of oncogenic K-Ras
Article Snippet: .. GST-tagged Ras-binding domain of C-Raf (Raf-RBD; a gift from Channing Der, Addgene_13338) was expressed in BL21-AI cells as described by Taylor et al. with changes adapted from Johnson et al. After induction with 1mM Isopropyl β-D-1-thiogalactopyranoside (IPTG) bacteria were grown at 37°C for 4 h with shaking. .. Absorbance at 450 nm was read using the GloMax Explorer Microplate Reader (Promega, #GM3500).

Recombinant:

Software:

Article Title: Regulation of GTPase function by autophosphorylation.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 50-CTTCCTGGCCGGTGGTAT CCAGAATG-30 Integrated DNA Technologies pET21 KRAS4B A59T Reverse 50-GACATTCTGGATACCGAAGGC CAGGAAGAGTATAG-30 Integrated DNA Technologies pET21 KRAS4B A59E Forward 50-CTATACTCTTCCTGGCCTTCGG TATCCAGAATGTC-30 Integrated DNA Technologies pET21 KRAS4B A59E Reverse 50-CACCGTTCTCGACACAGCAGGT CAAG-30 Integrated DNA Technologies SaCas9 gRNA 1 Forward 50-AAACCTTGACCTGCTGTGTC GAGAAC-30 Integrated DNA Technologies SaCas9 gRNA 1 Reverse 50-TGTGACCATTAGCATTGT TTGC-30 Integrated DNA Technologies mES Ex2 genotyping Forward primer 50-CTTAAACCCACCTATAAT GGTG-30 Integrated DNA Technologies mES Ex2 genotyping Reverse primer 50-AGTAATTGATGGAGAAA CCTG-30 Integrated DNA Technologies mES Ex2 Sequencing Forward primer 50-ATAATGGTGAATATCTT CAAA-30 Integrated DNA Technologies mES Ex2 Sequencing Reverse primer 50-CTGTAATAAT CCAGACTGTG TTTCTCCCTT CTCAGGACTCC TACAGGAAAC AAGTAGTAAT TGATGGAGAA ACCTGTCTCT TGGATATCCT CGACACAACA GGTCAAGAAG AGTACAGTGC AATGAGGGAC CAGTACATGA GAACTGGGGA GGGCTTTCTT TGTGTATT TGCCATAAA TAATACTAA ATCATTTG AAGAT-30 Integrated DNA Technologies Repair template for CRISPR experiments Recombinant DNA 100 bp DNA ladder New England Biolabs Cat#: N3231L 6xHis-GAP (715-1047) This study N/A 6xHis-K-RAS4B This study N/A 6xHis-SOS11 (564-1049) This study N/A Gateway pDEST15 vector Thermo Scientific Cat#: 11802014 H-RAS (1-166) Buhrman et al., 2010 N/A pBABE-hygro vector Addgene Cat#: 1765 pCMV5-Venus-K-Ras4B Terrell et al., 2019 N/A pET-21a(+) vector EMD Biosciences https://www.emdmillipore.com/US/en/ product/pET-21+-DNA-Novagen,EMD_ BIO-69770www.emdmillipore.com/US/ en/product/pET-21+-DNA-Novagen, EMD_BIO-69770 pET28 vector EMD Biosciences https://www.emdmillipore.com/US/en/ product/pET-28a+-DNA-Novagen,EMD_ BIO-69864www.emdmillipore.com/US/ en/product/pET-28a+-DNA-Novagen, EMD_BIO-69864 pGEX-4T2-A-RAF(17-94) Smith and Ikura, 2014 N/A pGEX-4T2-B-RAF(150-233) Smith and Ikura, 2014 N/A pGEX-4T2-C-RAF(53-137) Fang et al., 2020 N/A pGEX-4T2-RASSF5(199-367) Smith and Ikura, 2014 N/A pLHCX-WT-RafReg-Rluc8 (A-, B-, C-Raf) Terrell et al., 2019 N/A (Continued on next page) e4 Molecular Cell 82, 950–968.e1–e14, March 3, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER pX601-AAV-CMV::NLS-SaCas9-NLS3xHA-bGHpA;U6::BsaI-sgRNA Addgene Cat#: 61591 Raf1-RBD Addgene Cat#: 13338 RAS pathway collection v2.0 Addgene Kit#: 1000000070 RASSF1 Addgene Cat#: 70535 RASSF10 Addgene Cat#: 70537 RASSF2 Addgene Cat#: 70539 RASSF3 Addgene Cat#: 70541 RASSF4 Addgene Cat#: 70543 RASSF5 Addgene Cat#: 70545 RASSF6 Addgene Cat#: 70547 RASSF7 Addgene Cat#: 70549 RASSF8 Addgene Cat#: 70551 RASSF9 Addgene Cat#: 70553 Software and algorithms CHARM-GUI (solution builder) Brooks et al., 2009; Jo et al., 2008; Lee et al., 2016 https://charmm-gui.org/?doc=input/ solution Chimera (ver. .. Molecular dynamics simulations were conducted on the HMS O2 High Performance Compute Cluster (https://it.hms.harvard.edu/ our-services/research-computing).

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