anti raf1 (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc anti raf1
Anti Raf1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 643 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/raf1/c-Raf+Antibody/pm41664072-84-28-30
Average 96 stars, based on 643 article reviews

anti raf1 - by Bioz Stars, 2026-10

96/100 stars

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Blocking Assay:

Article Title: Bromodomain-containing protein 4 knockdown promotes neuronal ferroptosis in a mouse model of subarachnoid hemorrhage
Article Snippet: The protein samples were then separated using the One-Step PAGE Gel Fast Preparation kit (Vazyme, Nanjin, Jiangsu, China; Cat# E302, E304) and transferred onto polyvinylidene difluoride membranes. .. After blocking at ambient temperature for 2 hours, the membranes were incubated with the following primary antibodies for at least 10 hours at 4°C: rabbit-anti-BRD4 (1:200, Abcam, Cambridge, UK; Cat# ab243862, RRID: AB_3096484), mouse anti-glutathione peroxidase 4 (GPX4; 1:1000, Proteintech, Wuhan, Hubei, China; Cat# 67763-1-Ig, RRID: AB_2909469), rabbit anti-solute carrier family 7 member 11 (SLC7A11; Proteintech; Cat# 26864-1-AP, RRID: AB_2880661), rabbit anti-coenzyme Q10 homolog B (CoQ10B; 1:1000, ThermoFisher Scientific; Cat# PA5-90284, RRID: AB_2806000), rabbit anti-SOSTM1/P62 (1:1000, Abclonal, Chongqing, China; Cat# A19700, RRID: AB_2862742), rabbit anti-microtubule-associated proteins 1A/1B light chain 3B (LC3B; 1:1000, Proteintech; Cat# 14600-1-AP, RRID: AB_2137737), rabbit anti-ferritin heavy chain (FTH1; 1:1000, Abmart, Shanghai, China; Cat# TD6278S, RRID: AB_3096423), rabbit anti-Raf-1 (1:1000, Cell Signaling Technology, Danvers, MA, USA; Cat# 9422T, RRID: AB_390808), rabbit anti-p44/42 MAPK (Erk1/2; 1:3000, Cell Signaling Technology; Cat# 4695T, RRID: AB_390779), rabbit anti-phospho-p44/42 MAPK (Thr202/Tyr204) (p-Erk1/2; 1:5000, Cell Signaling Technology; Cat# 4370S, RRID: AB_2315112), rabbit anti-β-actin (1:2000, Proteintech; Cat# 20536-1-AP, RRID: AB_10700003), and mouse anti-β-actin (1:2000, Proteintech; Cat# 66009-1-Ig, RRID: AB_2687938). .. The membranes were then incubated with HRP-conjugated Affinipure goat anti-rabbit IgG(H+L) (1:2000, Proteintech; Cat# SA00001-2, RRID: AB_2722564) or HRP-conjugated Affinipure goat anti-mouse IgG(H+L) (1:2000, Proteintech; Cat# SA00001-1, RRID: AB_2722565) secondary antibodies for 2 hours at room temperature.

Incubation:

Article Title: GPR35 protects against reperfusion injury in ischemic stroke by binding with the CR2 domain of Raf1.
Article Snippet: Mouse brain protein lysates were resolved using 10% SDS-PAGE and electrophoretically transferred onto Bio TraceTM NT nitrocellulose membranes (Pall, #66485). .. Membranes were blocked at room temperature for 2 h using bovine serum albumin and subsequently incubated overnight at 4°C with the indicated primary antibodies: anti-GPR35 (1:1000, Novus, NBP2-24640), anti-Raf1 (1:1000, CST, 9422S), anti-phospho-ERK/anti-ERK (1:1000, Affinity, AF1015/AF0155), anti-phospho-JNK/anti-JNK (1:1000, Affinity, AF6138/AF3318), anti-phospho-p38 MAPK/anti-p38 MAPK (1:1000, Affinity, AF4001/AF6456), anti-phospho-AKT/anti-AKT (1:1000, HUABIO, HA721870/ET1607), anti-FLAG/anti-HA (1:5000, HUABIO, M1403-2/HA721750), and anti-β-actin/anti-GAPDH (1:100000/1:10000, ABclonal, AC026/AC001). ..

Planar Chromatography:

Article Title: HIIT-induced lactate/GPR81 signaling with dual branches converging on ERK1/2 contributes to hippocampal synaptic remodeling and memory improvement
Article Snippet: After a 15-min blocking step (PS108P, Epizyme, Shanghai, China), the samples were incubated with primary antibodies overnight at 4 °C. .. GPR81, PA5-114741, Thermo; AC (55067-1-AP, Proteintech), PKA (4782S, CST), RAP1 (2399S, CST), PKC (SC-80 Santa), PLC (14247S, CST), Raf1 (9422S, CST), MSK1 (3489S, CST), P90RSK (9355S, CST), ERK (4370T, CST), CREB (9197S, CST), c-Fos (2250S, CST), Zif268 (4154S, CST), NMDAR (5704S, CST), SYN (4329S, CST), BDNF (28205-1-AP, Proteintech), ARC (16290-1-AP, Proteintech), PSD95 (3450S, CST) p-Raf1 (9421S, CST), p-ERK (4370T, CST), p-MSK (9595S, CST), p-P90RSK (11989S, CST), p-CREB (9198S, CST), Tubulin (10068-1-AP, Proteintech), HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H + L) (SA00001-2, Proteintech), HRP-conjugated Affinipure Goat Anti-Mouse IgG (H + L) (SA00001-1, Proteintech). ..

other:

Article Title: Proteomics- and BRET- screens identify SPRY2 as a Ras effector that impacts its membrane organization
Article Snippet: Rabbit polyclonal anti-C-Raf , Cell Signaling Technology , 9422; RRID: AB_390808.

Review