raf1 antibody (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc raf1 antibody
Raf1 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 75 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/raf1/c-Raf+Mouse+mAb/pm41638654-40-70-73
Average 93 stars, based on 75 article reviews

raf1 antibody - by Bioz Stars, 2026-10

93/100 stars

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Article Title: Identification and characterization of binders to a novel cryptic and functional pocket in KRAS
Article Snippet: Detection was performed using the WesternBright ECL kit (Advansta K-551 12045-D20) with a Fusion Fx device.

Article Title: CAMK2 Expression and Its Regulation on Testosterone Synthesis in Mouse Testis.
Article Snippet: Antibodies and Inhibitors CAMK2γ antibody (12666- 2-AP; Proteintech Group, Inc.; Wuhan, China), phosphoCAMK2β/γ/δ antibody (YP0781; ImmunoWay; San Jose, CA, U.S.A.), 3β-HSD antibody (sc-30820; Santa Cruz Biotechnology; Santa Cruz, CA, U.S.A.), StAR antibody (12225-1-AP; Proteintech Group, Inc.), GAPDH antibody (M20006; Abmart, Shanghai, China), Phospho-p44/42 MAPK(ERK1/2) antibody (9101, Cell Signaling Technology, Boston, MA, U.S.A.), ERK1/2 antibody (4695, Cell Signaling Technology), PhosphoEGFR Antibody (30277-1-AP; Proteintech Group, Inc.), EGFR Antibody (18986-1-AP; Proteintech Group, Inc.), RAF1 Antibody (12552; Cell Signaling Technology).

Article Title: Structure of SHOC2-KRAS-PP1C complex reveals RAS isoform-specific determinants and insights into targeting complex assembly by RAS inhibitors.
Article Snippet: Table 2: Antibodies used in this study Antibodies Dilution Source Myc-tag Rabbit polyclonal Ab 1:2000 Cell Signaling Technology Cat.# 2272 PP1 alpha Rabbit polyclonal Ab 1:2000 Upstate Cat.# 06-221 FLAG Rabbit polyclonal Ab 1:4000 Sigma-Aldrich Cat.# F7425 CRAF Mouse mAb 1:2000 BD Biosciences Cat.# 610152 P-S259 RAF1 Rabbit polyclonal Ab 1:2000 Cell Signaling Technology Cat.# 9421 Anti-Rabbit IgG (H&L) Antibody DyLight 800 1:15,000 Invitrogen Cat.# SA5-35571 Anti-Mouse IgG (light chain) antibody Alexa Fluor 680 1:15,000 Jackson ImmunoResearch Cat.# 115-625174 Data availability The atomic coordinates and structure factors have been deposited in the Protein Data Bank and can be accessed using accession numbers 9O65 [http://doi.org/10.2210/pdb9O65/pdb], EMD70159 [https://www.ebi.ac.uk/emdb/EMD-70159] (stabilized SKP complex), 9O0N [http://doi.org/10.2210/pdb9O0N/pdb] (KRAS(1-169)GDP with MRTX1133), 9O0O [http://doi.org/10.2210/pdb9O0O/pdb] (KRAS (1-169)GMPPNP with MRTX1133), 9O0P [http://doi.org/10.2210/pdb9O0P/pdb] (MRASmut(1-178)GDP with MRTX1133) and 9O0Q [http://doi.org/10.2210/pdb9O0Q/pdb] (MRASmut(1-178)GMPPNP with MRTX1133).

Article Title: BRAF V600E -Driven Lung Tumorigenesis Requires Ligand-Mediated Activation of ERBB Receptor Signaling
Article Snippet: Anti-pY1068/pY1173-EGFR (1H12/2236S); Cell Signaling (Catalog# 53A5/4407S; RRID: AB_331795).

Article Title: KRAS-driven pancreatic ductal adenocarcinoma cell lines harbour putative cancer stem cells
Article Snippet: The primary antibodies used include p-CRAF (Cat# 9427, RRID: AB_2067317, Cell Signalling Technology, USA), CRAF (Cat# 12552, RRID: AB_2728706, Cell Signalling Technology), p-ERK1/2 (Cat# 9106, RRID: AB_331768, Cell Signalling Technology), ERK1/2 (Cat# 4696, RRID: AB_390780, Cell Signalling Technology), p-AKT (Cat# 4060, RRID: AB_2315049, Cell Signalling Technology), AKT (Cat# 4685, RRID: AB_2225340, Cell Signalling Technology), p-NF-κB P65 (Cat# 3033, RRID: AB_331284, Cell Signalling Technology), NF-κB P65 (Cat# 8242, RRID: AB_10859369, Cell Signalling Technology), p-GSK3β (Cat# 5558, RRID: AB_10013750, Cell Signalling Technology), GSK3β (Cat# 12456, RRID: AB_2636978, Cell Signalling Technology), SOX2 (Cat# 3579, RRID: AB_2195767, Cell Signalling Technology), OCT4 (Cat# 2750, RRID: AB_823583, Cell Signalling Technology), GAPDH (Cat# 5174, RRID: AB_10622025, Cell Signalling Technology), and β-actin (Cat# 4970, RRID: AB_2223172, Cell Signalling Technology).

Article Title: Dual-specificity phosphatase 6 interferes with the repressive activity of forkhead box O1 towards CYP4A11 that mediates lipid accumulation in the liver
Article Snippet: Anti-phospho-p44/42 MAPK (Erk1/2) (1:1000, Thr202/Tyr204; p-ERK) (D13.14.4E, #4370S), anti-ERK (1:1000, 137F5, #4695S), anti-phospho-AKT (1:1000, Ser473; p-AKT) (D9E, #4060S), anti-AKT (1:1000, C67E7, #4691S), anti-FOXO1 (1:1000, C29H4, #2880S), anti-phospho-FOXO1 (Ser256) (1:1000, #9461S), anti-GAPDH (1:1000, D16H11, #5174S), anti-MTOR (1:1000, 7C10, #2983S), anti-phospho-c-RAF (Ser259; p-RAF) (1:1000,#9421S), and anti-RAF (1:1000, D5 × 6R, #12552S) were purchased from Cell Signaling Technology (Danvers, MA, USA).

Article Title: Polo-like Kinase 1 Predicts Lymph Node Metastasis in Middle Eastern Colorectal Cancer Patients; Its Inhibition Reverses 5-Fu Resistance in Colorectal Cancer Cells
Article Snippet: Antibodies against pCRAF (9427), CRAF (12552), PLK1 (4513), MEK1/2 (4694), pMEK1/2 (9121), ERK1/2 (4695), pERK1/2 (4370), E-cadherin (3195), Zeb1 (3396), CD133 (64326), CD44 (3570), NANOG (4903), and GAPDH (5174) were obtained from Cell Signaling Technology (Danvers, MA, USA).

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Article Title: Combination of the Novel RAF Dimer Inhibitor Brimarafenib With the MEK Inhibitor Mirdametinib Is Effective Against NRAS Mutant Melanoma
Article Snippet: Immunoprecipitation was performed using protein A/G magnetic beads (Thermo Fischer Scientific, 78609) pre‐washed with blocking buffer (PBS, 1% bovine serum albumin (BSA) and 0.1% Tween‐20). .. Samples were eluted, heated at 80°C, and analyzed by Western Blotting using antibodies against MEK1/2 (CST, #8727), phospho‐MEK1/2 (CST, #9154) and RAF‐1 (CRAF, CST, #12552S). .. High‐quality RNA was extracted using the “high pure RNA isolation kit” (Roche, Cat#11828665001).

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