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Santa Cruz Biotechnology raf1
Raf1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 909 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/raf1/Raf-1+Antibody/pm41664072-93-21-24
Average 94 stars, based on 909 article reviews
raf1 - by Bioz Stars, 2026-09
94/100 stars

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Related Articles

Incubation:

Article Title: Spatiotemporal regulation of MKK4 dictates switch-like JNK activation and binary cell-fate decisions.
Article Snippet: .. The cells were washed twice with PBS, permeabilized with 0.1% Triton X-100 for 5min, and incubated in blocking solution BlockAce (Snow BrandMilk Products) for 1 h, and then incubated with the appropriate antibody [anti-Flag M2 (Sigma-Aldrich, F1804, 1:500 dilution); anti-Myc 9E10 (sc-40, 1:500 dilution), anti-MEK1 H-8 (sc6250, 1:200 dilution), anti-Raf-1 E-10 (sc-7267, 1:200 dilution) (Santa Cruz Biotechnology); anti-MKK4 5C10 (Cell Signaling Technology, 3346, 1:100 dilution); and anti-MTK1 antibody (made in-house, 1:200 dilution)] at 4 °C overnight in PBS containing 2% BSA. .. Following three washes in PBS, the cells were incubated with appropriate secondary antibodies [Alexa Fluor 488-conjugated anti-mouse IgG antibody (A11029, 1:500 dilution); Alexa Fluor 568-conjugated anti-mouse IgG antibody (A11019, 1:500 dilution); Alexa Fluor 568-conjugated antirabbit IgG antibody (A11036, 1:500dilution) (ThermoFisher Scientific); Alexa Fluor 488-conjugated anti-rabbit IgG antibody (Jackson ImmunoResearch Labs, Cat# 711-545-152, 1:500 dilution)] and DAPI (25 ng/ ml) for 30min.

Article Title: Ginsenoside Rg3 synergizes with near-infrared photothermal therapy to suppress prostate cancer progression by inhibiting RAS signaling and enhancing ARL11-mediated macrophage reprogramming.
Article Snippet: Prostate cancer (PCa) progression and therapeutic resistance are largely driven by aberrant oncogenic signaling and an immunosuppressive microenvironment.. Ginsenoside Rg3, a natural saponin from Panax ginseng, exhibits antitumor and immunomodulatory activity, but its therapeutic efficacy is limited when used as a monotherapy.. Here, we investigated the synergistic potential of combining Rg3 with near-infrared (NIR) exposure in PCa.

Article Title: GPR35 protects against reperfusion injury in ischemic stroke by binding with the CR2 domain of Raf1.
Article Snippet: .. Sections were incubated with NeuN (1:100, 94403, CST), Iba1 (1:100, MA527726, Invitrogen), GFAP (1:200, GB12096-100, Servicebio), CD68 (1:200, ab283654, Abcam), or Raf1 (1:100, sc7267, Santa Cruz), followed by DyLight-conjugated secondary antibodies (1:200, E032210-01, E032420-01, EarthOx) and DAPI counterstaining. ..

Blocking Assay:

Article Title: Spatiotemporal regulation of MKK4 dictates switch-like JNK activation and binary cell-fate decisions.
Article Snippet: .. The cells were washed twice with PBS, permeabilized with 0.1% Triton X-100 for 5min, and incubated in blocking solution BlockAce (Snow BrandMilk Products) for 1 h, and then incubated with the appropriate antibody [anti-Flag M2 (Sigma-Aldrich, F1804, 1:500 dilution); anti-Myc 9E10 (sc-40, 1:500 dilution), anti-MEK1 H-8 (sc6250, 1:200 dilution), anti-Raf-1 E-10 (sc-7267, 1:200 dilution) (Santa Cruz Biotechnology); anti-MKK4 5C10 (Cell Signaling Technology, 3346, 1:100 dilution); and anti-MTK1 antibody (made in-house, 1:200 dilution)] at 4 °C overnight in PBS containing 2% BSA. .. Following three washes in PBS, the cells were incubated with appropriate secondary antibodies [Alexa Fluor 488-conjugated anti-mouse IgG antibody (A11029, 1:500 dilution); Alexa Fluor 568-conjugated anti-mouse IgG antibody (A11019, 1:500 dilution); Alexa Fluor 568-conjugated antirabbit IgG antibody (A11036, 1:500dilution) (ThermoFisher Scientific); Alexa Fluor 488-conjugated anti-rabbit IgG antibody (Jackson ImmunoResearch Labs, Cat# 711-545-152, 1:500 dilution)] and DAPI (25 ng/ ml) for 30min.



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