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human cartilage oligomeric matrix protein  (OriGene)


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    Structured Review

    OriGene human cartilage oligomeric matrix protein
    Human Cartilage Oligomeric Matrix Protein, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/psectag2b+expression+vector/COMP+(NM_000095)+Human+Recombinant+Protein/pm37338522-224-59-31
    Average 91 stars, based on 1 article reviews
    human cartilage oligomeric matrix protein - by Bioz Stars, 2026-10
    91/100 stars

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    Related Articles

    Expressing:

    Article Title: Polydom/SVEP1 binds to Tie1 and promotes migration of lymphatic endothelial cells.
    Article Snippet: A cDNA segment encoding the extracellular region of human Podoplanin (amino acids 23–131) was amplified by PCR using MGC clone 4876446 (Open Biosystems) as a template and inserted into pSecTag2B-Fc-FLAG at the HindIII/NotI sites. cDNA segments encoding full-length human Tie1 (amino acids 1–1138) and Tie2 (amino acids 1–1124) were obtained by RT-PCR using RNA extracted from human LECs and inserted with a cDNA segment encoding C-terminal FLAG tag (DYKDDDDK) or HA tag (YPYDVPDYA), respectively, into the pcDNA3.1 vector at the KpnI/ApaI sites. .. The expression vector for COMP-Ang1 was constructed as follows: a cDNA segment encoding human Ang1 linker and fibrinogen-like domain (amino acids 255–498) was amplified by PCR using human cDNA clone SC111587 (OriGene Technologies) as a template and inserted into pSecTag2B at the HindIII/ApaI sites with the cDNA segment encoding an N-terminal FLAG tag and a short coiled-coil domain of human cartilage oligomeric matrix protein (COMP; amino acids 28–73, amplified by PCR using synthetic primers; Cho et al., 2004). .. Expression and purification of recombinant proteins Recombinant Polydom, Polydom fragments, FLAG-tagged Fcfusion proteins, and FLAG-tagged COMP-Ang1 were produced using a Freestyle 293 Expression System (Thermo Fisher Scientific).

    Plasmid Preparation:

    Article Title: Polydom/SVEP1 binds to Tie1 and promotes migration of lymphatic endothelial cells.
    Article Snippet: A cDNA segment encoding the extracellular region of human Podoplanin (amino acids 23–131) was amplified by PCR using MGC clone 4876446 (Open Biosystems) as a template and inserted into pSecTag2B-Fc-FLAG at the HindIII/NotI sites. cDNA segments encoding full-length human Tie1 (amino acids 1–1138) and Tie2 (amino acids 1–1124) were obtained by RT-PCR using RNA extracted from human LECs and inserted with a cDNA segment encoding C-terminal FLAG tag (DYKDDDDK) or HA tag (YPYDVPDYA), respectively, into the pcDNA3.1 vector at the KpnI/ApaI sites. .. The expression vector for COMP-Ang1 was constructed as follows: a cDNA segment encoding human Ang1 linker and fibrinogen-like domain (amino acids 255–498) was amplified by PCR using human cDNA clone SC111587 (OriGene Technologies) as a template and inserted into pSecTag2B at the HindIII/ApaI sites with the cDNA segment encoding an N-terminal FLAG tag and a short coiled-coil domain of human cartilage oligomeric matrix protein (COMP; amino acids 28–73, amplified by PCR using synthetic primers; Cho et al., 2004). .. Expression and purification of recombinant proteins Recombinant Polydom, Polydom fragments, FLAG-tagged Fcfusion proteins, and FLAG-tagged COMP-Ang1 were produced using a Freestyle 293 Expression System (Thermo Fisher Scientific).

    Construct:

    Article Title: Polydom/SVEP1 binds to Tie1 and promotes migration of lymphatic endothelial cells.
    Article Snippet: A cDNA segment encoding the extracellular region of human Podoplanin (amino acids 23–131) was amplified by PCR using MGC clone 4876446 (Open Biosystems) as a template and inserted into pSecTag2B-Fc-FLAG at the HindIII/NotI sites. cDNA segments encoding full-length human Tie1 (amino acids 1–1138) and Tie2 (amino acids 1–1124) were obtained by RT-PCR using RNA extracted from human LECs and inserted with a cDNA segment encoding C-terminal FLAG tag (DYKDDDDK) or HA tag (YPYDVPDYA), respectively, into the pcDNA3.1 vector at the KpnI/ApaI sites. .. The expression vector for COMP-Ang1 was constructed as follows: a cDNA segment encoding human Ang1 linker and fibrinogen-like domain (amino acids 255–498) was amplified by PCR using human cDNA clone SC111587 (OriGene Technologies) as a template and inserted into pSecTag2B at the HindIII/ApaI sites with the cDNA segment encoding an N-terminal FLAG tag and a short coiled-coil domain of human cartilage oligomeric matrix protein (COMP; amino acids 28–73, amplified by PCR using synthetic primers; Cho et al., 2004). .. Expression and purification of recombinant proteins Recombinant Polydom, Polydom fragments, FLAG-tagged Fcfusion proteins, and FLAG-tagged COMP-Ang1 were produced using a Freestyle 293 Expression System (Thermo Fisher Scientific).

    Amplification:

    Article Title: Polydom/SVEP1 binds to Tie1 and promotes migration of lymphatic endothelial cells.
    Article Snippet: A cDNA segment encoding the extracellular region of human Podoplanin (amino acids 23–131) was amplified by PCR using MGC clone 4876446 (Open Biosystems) as a template and inserted into pSecTag2B-Fc-FLAG at the HindIII/NotI sites. cDNA segments encoding full-length human Tie1 (amino acids 1–1138) and Tie2 (amino acids 1–1124) were obtained by RT-PCR using RNA extracted from human LECs and inserted with a cDNA segment encoding C-terminal FLAG tag (DYKDDDDK) or HA tag (YPYDVPDYA), respectively, into the pcDNA3.1 vector at the KpnI/ApaI sites. .. The expression vector for COMP-Ang1 was constructed as follows: a cDNA segment encoding human Ang1 linker and fibrinogen-like domain (amino acids 255–498) was amplified by PCR using human cDNA clone SC111587 (OriGene Technologies) as a template and inserted into pSecTag2B at the HindIII/ApaI sites with the cDNA segment encoding an N-terminal FLAG tag and a short coiled-coil domain of human cartilage oligomeric matrix protein (COMP; amino acids 28–73, amplified by PCR using synthetic primers; Cho et al., 2004). .. Expression and purification of recombinant proteins Recombinant Polydom, Polydom fragments, FLAG-tagged Fcfusion proteins, and FLAG-tagged COMP-Ang1 were produced using a Freestyle 293 Expression System (Thermo Fisher Scientific).

    Polymerase Chain Reaction:

    Article Title: Polydom/SVEP1 binds to Tie1 and promotes migration of lymphatic endothelial cells.
    Article Snippet: A cDNA segment encoding the extracellular region of human Podoplanin (amino acids 23–131) was amplified by PCR using MGC clone 4876446 (Open Biosystems) as a template and inserted into pSecTag2B-Fc-FLAG at the HindIII/NotI sites. cDNA segments encoding full-length human Tie1 (amino acids 1–1138) and Tie2 (amino acids 1–1124) were obtained by RT-PCR using RNA extracted from human LECs and inserted with a cDNA segment encoding C-terminal FLAG tag (DYKDDDDK) or HA tag (YPYDVPDYA), respectively, into the pcDNA3.1 vector at the KpnI/ApaI sites. .. The expression vector for COMP-Ang1 was constructed as follows: a cDNA segment encoding human Ang1 linker and fibrinogen-like domain (amino acids 255–498) was amplified by PCR using human cDNA clone SC111587 (OriGene Technologies) as a template and inserted into pSecTag2B at the HindIII/ApaI sites with the cDNA segment encoding an N-terminal FLAG tag and a short coiled-coil domain of human cartilage oligomeric matrix protein (COMP; amino acids 28–73, amplified by PCR using synthetic primers; Cho et al., 2004). .. Expression and purification of recombinant proteins Recombinant Polydom, Polydom fragments, FLAG-tagged Fcfusion proteins, and FLAG-tagged COMP-Ang1 were produced using a Freestyle 293 Expression System (Thermo Fisher Scientific).

    FLAG-tag:

    Article Title: Polydom/SVEP1 binds to Tie1 and promotes migration of lymphatic endothelial cells.
    Article Snippet: A cDNA segment encoding the extracellular region of human Podoplanin (amino acids 23–131) was amplified by PCR using MGC clone 4876446 (Open Biosystems) as a template and inserted into pSecTag2B-Fc-FLAG at the HindIII/NotI sites. cDNA segments encoding full-length human Tie1 (amino acids 1–1138) and Tie2 (amino acids 1–1124) were obtained by RT-PCR using RNA extracted from human LECs and inserted with a cDNA segment encoding C-terminal FLAG tag (DYKDDDDK) or HA tag (YPYDVPDYA), respectively, into the pcDNA3.1 vector at the KpnI/ApaI sites. .. The expression vector for COMP-Ang1 was constructed as follows: a cDNA segment encoding human Ang1 linker and fibrinogen-like domain (amino acids 255–498) was amplified by PCR using human cDNA clone SC111587 (OriGene Technologies) as a template and inserted into pSecTag2B at the HindIII/ApaI sites with the cDNA segment encoding an N-terminal FLAG tag and a short coiled-coil domain of human cartilage oligomeric matrix protein (COMP; amino acids 28–73, amplified by PCR using synthetic primers; Cho et al., 2004). .. Expression and purification of recombinant proteins Recombinant Polydom, Polydom fragments, FLAG-tagged Fcfusion proteins, and FLAG-tagged COMP-Ang1 were produced using a Freestyle 293 Expression System (Thermo Fisher Scientific).



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    Image Search Results


    Journal: Cell Host & Microbe

    Article Title: A Potent Germline-like Human Monoclonal Antibody Targets a pH-Sensitive Epitope on H7N9 Influenza Hemagglutinin

    doi: 10.1016/j.chom.2017.08.011

    Figure Lengend Snippet:

    Article Snippet: The HA1 gene of A/Shanghai/2/2013 was synthesized (Genscript, Nanjing, China), fused with the Fc fragment of human IgG1 and a C-terminal Avi-tag (GLNDIFEAQKIEWHE), and cloned into pSecTag2B expression vector (Thermo Fisher).

    Techniques: Recombinant, Electron Microscopy, Affinity Chromatography, Integrity Assay, Expressing, Plasmid Preparation, Software