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protoscript ii first strand cdna synthesis kit  (New England Biolabs)


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    Structured Review

    New England Biolabs protoscript ii first strand cdna synthesis kit
    Protoscript Ii First Strand Cdna Synthesis Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1843 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/protoscript+kit/ProtoScript+II+First+Strand+cDNA+Synthesis+Kit/pmc13612924-68-4-11
    Average 99 stars, based on 1843 article reviews
    protoscript ii first strand cdna synthesis kit - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    cDNA Synthesis:

    Article Title: Pampa biome plant extracts and flavonoids show antiviral activity against Bovine Herpesvirus and Bovine Viral Diarrhea Virus.
    Article Snippet: The final RNA was eluted in 30 μL of ultrapure sterile water (RMBIO) and quantified using a NanoDropTM One Spectrophotometer (Thermo ScientificTM). .. Samples were stored at –80 °C until use. cDNA synthesis was performed using the ProtoScript® II First Strand cDNA Synthesis Kit (New England Biolabs Inc.), following the manufacturer’s instructions. ..

    Article Title: Functional Characterization of OTU Domain-Containing Deubiquitinases from Plant Pathogenic Fungi Reveals Distinct Immune Modulatory Mechanisms
    Article Snippet: .. First-strand cDNA was synthesized from 750 ng of total RNA using random primers and reverse transcriptase (ProtoScript II First Strand cDNA Synthesis Kit, NEB, Ipswich, MA, USA). ..

    Article Title: Chromatin modifiers KMT2D, cBAF, and p300 cooperatively facilitate de novo binding of transcription factors on enhancers
    Article Snippet: .. Total RNA was extracted using TRIzol (#15596018, Invitrogen) and reverse transcribed using ProtoScript II first-strand cDNA synthesis kit (#E6560,NEB), followingmanufacturer’s instructions.QuantitativeRTPCR (qRT-PCR) was performed in triplicate with the Luna Universal qPCR Master Mix (#M3003, NEB) using QuantStudio 5 Real-Time PCR System (Applied Biosystems). ..

    Article Title: Dietary α-ketoglutarate modulates lung adenocarcinoma growth and epigenetic remodeling in a sex-dependent fashion.
    Article Snippet: .. First-strand cDNA synthesis was performed using the NEB ProtoScript II First Strand cDNA Synthesis Kit (E6560) according to the manufacturer’s protocol. ..

    Article Title: The NORAD -Pumilio regulatory axis in the evolution of inclusion body myositis
    Article Snippet: Total RNA was extracted from frozen muscle blocks (20-μm-thick sections, 20 sections per block) using Mini RNeasy Plus Universal Kit (Qiagen, 74134). .. cDNAs were synthesized using ProtoScript II First Strand cDNA Synthesis Kit (New England BioLabs, E6560L). .. Primer sets (synthesized by IDT) for NORAD (human, F: AAGCTGCTCTCAACTCCACC, R: GGACGTATCGCTTCCAGAGG; mouse, F: AGACCTCATCCTGTGAACCT, R: CCTCTTGAA CATGGAACTGCTA), PUM1 (human, F: CTTCAAGACACATGCAACGC, R: TTGTCGGAGTGAAAGGTAAGG; mouse, F: CTTCAAGACACATGCAACGC, R: TTGTCGGAGTGAAAGGTAAGG); and PUM2 (human, F: TCGACCACTACCATTTGTCAG, R: TTCCTCCTCTCCAAGTCCAA; mouse, F: AGAGATATACCGACCACTACCG, R: CAAGCCAAACTCCAGGACAT).

    Article Title: Premature cleavage and polyadenylation in the minor intron of PTEN modulate its expression and generate a functional long non-coding RNA
    Article Snippet: Protein concentrations were measured using the Bio-Rad protein assay. .. For reverse transcription, 1 μg of RNA was converted to cDNA using the ProtoScript II First Strand cDNA Synthesis Kit (New England BioLabs), according to the manufacturer’s protocol. cDNA was then diluted 10-fold in nuclease-free water and used as input for PCR. ..

    Article Title: A splicing factor-cohesin association links RNA processing to cohesin-dependent genome folding.
    Article Snippet: RNA concentration and purity were assessed using a NanoDrop 2000 spectrophotometer (Thermo). .. Firststrand cDNA was synthesized from 1 μg total RNA using the ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs, #E6560). .. Quantitative PCR was performed using FastStart Universal SYBR Green Master (ROX) (Roche, #04913914001) on a qTOWER3 G touch real-time PCR system (Analytik Jena).

    Article Title: IGF-1Eb Isoform Immunoreactivity May Be Associated with Placental Dysfunction and Vascular Pathology in Fetal Growth Restriction (FGR).
    Article Snippet: .. Complementary DNA (cDNA) synthesis was carried out by reverse transcription using the ProtoScript II First Strand cDNA Synthesis Kit (New England BioLabs, Ipswich, MA, USA; cat. no. E6560L) according to the manufacturer’s instructions. .. The quality and purity of the extracted total RNA were assessed spectrophotometrically using a NanoDrop 2000 spectrophotometer (Thermo Scientific, Waltham, MA, USA) by measuring absorbance at 260 and 280 nm.

    Synthesized:

    Article Title: Functional Characterization of OTU Domain-Containing Deubiquitinases from Plant Pathogenic Fungi Reveals Distinct Immune Modulatory Mechanisms
    Article Snippet: .. First-strand cDNA was synthesized from 750 ng of total RNA using random primers and reverse transcriptase (ProtoScript II First Strand cDNA Synthesis Kit, NEB, Ipswich, MA, USA). ..

    Article Title: The NORAD -Pumilio regulatory axis in the evolution of inclusion body myositis
    Article Snippet: Total RNA was extracted from frozen muscle blocks (20-μm-thick sections, 20 sections per block) using Mini RNeasy Plus Universal Kit (Qiagen, 74134). .. cDNAs were synthesized using ProtoScript II First Strand cDNA Synthesis Kit (New England BioLabs, E6560L). .. Primer sets (synthesized by IDT) for NORAD (human, F: AAGCTGCTCTCAACTCCACC, R: GGACGTATCGCTTCCAGAGG; mouse, F: AGACCTCATCCTGTGAACCT, R: CCTCTTGAA CATGGAACTGCTA), PUM1 (human, F: CTTCAAGACACATGCAACGC, R: TTGTCGGAGTGAAAGGTAAGG; mouse, F: CTTCAAGACACATGCAACGC, R: TTGTCGGAGTGAAAGGTAAGG); and PUM2 (human, F: TCGACCACTACCATTTGTCAG, R: TTCCTCCTCTCCAAGTCCAA; mouse, F: AGAGATATACCGACCACTACCG, R: CAAGCCAAACTCCAGGACAT).

    Article Title: A splicing factor-cohesin association links RNA processing to cohesin-dependent genome folding.
    Article Snippet: RNA concentration and purity were assessed using a NanoDrop 2000 spectrophotometer (Thermo). .. Firststrand cDNA was synthesized from 1 μg total RNA using the ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs, #E6560). .. Quantitative PCR was performed using FastStart Universal SYBR Green Master (ROX) (Roche, #04913914001) on a qTOWER3 G touch real-time PCR system (Analytik Jena).

    Reverse Transcription:

    Article Title: Functional Characterization of OTU Domain-Containing Deubiquitinases from Plant Pathogenic Fungi Reveals Distinct Immune Modulatory Mechanisms
    Article Snippet: .. First-strand cDNA was synthesized from 750 ng of total RNA using random primers and reverse transcriptase (ProtoScript II First Strand cDNA Synthesis Kit, NEB, Ipswich, MA, USA). ..

    Article Title: Chromatin modifiers KMT2D, cBAF, and p300 cooperatively facilitate de novo binding of transcription factors on enhancers
    Article Snippet: .. Total RNA was extracted using TRIzol (#15596018, Invitrogen) and reverse transcribed using ProtoScript II first-strand cDNA synthesis kit (#E6560,NEB), followingmanufacturer’s instructions.QuantitativeRTPCR (qRT-PCR) was performed in triplicate with the Luna Universal qPCR Master Mix (#M3003, NEB) using QuantStudio 5 Real-Time PCR System (Applied Biosystems). ..

    Article Title: Premature cleavage and polyadenylation in the minor intron of PTEN modulate its expression and generate a functional long non-coding RNA
    Article Snippet: Protein concentrations were measured using the Bio-Rad protein assay. .. For reverse transcription, 1 μg of RNA was converted to cDNA using the ProtoScript II First Strand cDNA Synthesis Kit (New England BioLabs), according to the manufacturer’s protocol. cDNA was then diluted 10-fold in nuclease-free water and used as input for PCR. ..

    Article Title: IGF-1Eb Isoform Immunoreactivity May Be Associated with Placental Dysfunction and Vascular Pathology in Fetal Growth Restriction (FGR).
    Article Snippet: .. Complementary DNA (cDNA) synthesis was carried out by reverse transcription using the ProtoScript II First Strand cDNA Synthesis Kit (New England BioLabs, Ipswich, MA, USA; cat. no. E6560L) according to the manufacturer’s instructions. .. The quality and purity of the extracted total RNA were assessed spectrophotometrically using a NanoDrop 2000 spectrophotometer (Thermo Scientific, Waltham, MA, USA) by measuring absorbance at 260 and 280 nm.

    Real-time Polymerase Chain Reaction:

    Article Title: Chromatin modifiers KMT2D, cBAF, and p300 cooperatively facilitate de novo binding of transcription factors on enhancers
    Article Snippet: .. Total RNA was extracted using TRIzol (#15596018, Invitrogen) and reverse transcribed using ProtoScript II first-strand cDNA synthesis kit (#E6560,NEB), followingmanufacturer’s instructions.QuantitativeRTPCR (qRT-PCR) was performed in triplicate with the Luna Universal qPCR Master Mix (#M3003, NEB) using QuantStudio 5 Real-Time PCR System (Applied Biosystems). ..

    Polymerase Chain Reaction:

    Article Title: Premature cleavage and polyadenylation in the minor intron of PTEN modulate its expression and generate a functional long non-coding RNA
    Article Snippet: Protein concentrations were measured using the Bio-Rad protein assay. .. For reverse transcription, 1 μg of RNA was converted to cDNA using the ProtoScript II First Strand cDNA Synthesis Kit (New England BioLabs), according to the manufacturer’s protocol. cDNA was then diluted 10-fold in nuclease-free water and used as input for PCR. ..



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