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protoscript first strand cdna synthesis kit  (New England Biolabs)


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    Structured Review

    New England Biolabs protoscript first strand cdna synthesis kit
    Protoscript First Strand Cdna Synthesis Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1843 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/protoscript+kit/ProtoScript+II+First+Strand+cDNA+Synthesis+Kit/pm42129323-306-26-32
    Average 99 stars, based on 1843 article reviews
    protoscript first strand cdna synthesis kit - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Chromatin modifiers KMT2D, cBAF, and p300 cooperatively facilitate de novo binding of transcription factors on enhancers
    Article Snippet: .. Total RNA was extracted using TRIzol (#15596018, Invitrogen) and reverse transcribed using ProtoScript II first-strand cDNA synthesis kit (#E6560,NEB), followingmanufacturer’s instructions.QuantitativeRTPCR (qRT-PCR) was performed in triplicate with the Luna Universal qPCR Master Mix (#M3003, NEB) using QuantStudio 5 Real-Time PCR System (Applied Biosystems). ..

    Article Title: IGF-1Eb Isoform Immunoreactivity May Be Associated with Placental Dysfunction and Vascular Pathology in Fetal Growth Restriction (FGR).
    Article Snippet: .. Complementary DNA (cDNA) synthesis was carried out by reverse transcription using the ProtoScript II First Strand cDNA Synthesis Kit (New England BioLabs, Ipswich, MA, USA; cat. no. E6560L) according to the manufacturer’s instructions. .. The quality and purity of the extracted total RNA were assessed spectrophotometrically using a NanoDrop 2000 spectrophotometer (Thermo Scientific, Waltham, MA, USA) by measuring absorbance at 260 and 280 nm.

    cDNA Synthesis:

    Article Title: Chromatin modifiers KMT2D, cBAF, and p300 cooperatively facilitate de novo binding of transcription factors on enhancers
    Article Snippet: .. Total RNA was extracted using TRIzol (#15596018, Invitrogen) and reverse transcribed using ProtoScript II first-strand cDNA synthesis kit (#E6560,NEB), followingmanufacturer’s instructions.QuantitativeRTPCR (qRT-PCR) was performed in triplicate with the Luna Universal qPCR Master Mix (#M3003, NEB) using QuantStudio 5 Real-Time PCR System (Applied Biosystems). ..

    Article Title: Dietary α-ketoglutarate modulates lung adenocarcinoma growth and epigenetic remodeling in a sex-dependent fashion.
    Article Snippet: .. First-strand cDNA synthesis was performed using the NEB ProtoScript II First Strand cDNA Synthesis Kit (E6560) according to the manufacturer’s protocol. ..

    Article Title: Investigation of the Effect of the Tri-Set Training Method on Plasma mir-1, mir-128a, mir-133, and mir-206 Levels
    Article Snippet: .. To quantify miRNA expression levels, cDNA was synthesized from total RNA using stem-loop primers and the Proto Script II First Strand cDNA Synthesis (NEB) kit. ..

    Article Title: Exposure and Bioaccumulation of Mercury Impacted the Extent of Cellular Damage and Oxidative Stress in Local Chickens Around Ilesha Gold Mining Areas of Nigeria.
    Article Snippet: Mercury (Hg) is a wellknown global pollutant of environmental concern.. Uncontrolled mining activities represent an avenue for its release and possible environmental contamination.. Ilesha gold mining areas are characterized by extensive artisan and smallscale gold mining (ASGM) activities.

    Article Title: The NORAD -Pumilio regulatory axis in the evolution of inclusion body myositis
    Article Snippet: Total RNA was extracted from frozen muscle blocks (20-μm-thick sections, 20 sections per block) using Mini RNeasy Plus Universal Kit (Qiagen, 74134). .. cDNAs were synthesized using ProtoScript II First Strand cDNA Synthesis Kit (New England BioLabs, E6560L). .. Primer sets (synthesized by IDT) for NORAD (human, F: AAGCTGCTCTCAACTCCACC, R: GGACGTATCGCTTCCAGAGG; mouse, F: AGACCTCATCCTGTGAACCT, R: CCTCTTGAA CATGGAACTGCTA), PUM1 (human, F: CTTCAAGACACATGCAACGC, R: TTGTCGGAGTGAAAGGTAAGG; mouse, F: CTTCAAGACACATGCAACGC, R: TTGTCGGAGTGAAAGGTAAGG); and PUM2 (human, F: TCGACCACTACCATTTGTCAG, R: TTCCTCCTCTCCAAGTCCAA; mouse, F: AGAGATATACCGACCACTACCG, R: CAAGCCAAACTCCAGGACAT).

    Article Title: Csi-miR482a protected by food matrix ameliorates dextran sulfate sodium-induced colitis in mice via modulating gut microbes
    Article Snippet: .. The total RNA from the bacteria was extracted using TRNzol Universal reagent (Tiangen, Beijing, China), based on which cDNAs were synthesized using ProtoScript II First Strand cDNA Synthesis kit (New England Biolabs, Ipswich, USA). .. The qPCR was performed on a Bio-rad CFX96 Real-Time PCR Detection System (Hercules, California, USA), using TB Green Premix Ex Taq kit (Takara Bio, Nojihigashi, Japan) and gene specific primers.

    Article Title: A splicing factor-cohesin association links RNA processing to cohesin-dependent genome folding.
    Article Snippet: RNA concentration and purity were assessed using a NanoDrop 2000 spectrophotometer (Thermo). .. Firststrand cDNA was synthesized from 1 μg total RNA using the ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs, #E6560). .. Quantitative PCR was performed using FastStart Universal SYBR Green Master (ROX) (Roche, #04913914001) on a qTOWER3 G touch real-time PCR system (Analytik Jena).

    Article Title: IGF-1Eb Isoform Immunoreactivity May Be Associated with Placental Dysfunction and Vascular Pathology in Fetal Growth Restriction (FGR).
    Article Snippet: .. Complementary DNA (cDNA) synthesis was carried out by reverse transcription using the ProtoScript II First Strand cDNA Synthesis Kit (New England BioLabs, Ipswich, MA, USA; cat. no. E6560L) according to the manufacturer’s instructions. .. The quality and purity of the extracted total RNA were assessed spectrophotometrically using a NanoDrop 2000 spectrophotometer (Thermo Scientific, Waltham, MA, USA) by measuring absorbance at 260 and 280 nm.

    Real-time Polymerase Chain Reaction:

    Article Title: Chromatin modifiers KMT2D, cBAF, and p300 cooperatively facilitate de novo binding of transcription factors on enhancers
    Article Snippet: .. Total RNA was extracted using TRIzol (#15596018, Invitrogen) and reverse transcribed using ProtoScript II first-strand cDNA synthesis kit (#E6560,NEB), followingmanufacturer’s instructions.QuantitativeRTPCR (qRT-PCR) was performed in triplicate with the Luna Universal qPCR Master Mix (#M3003, NEB) using QuantStudio 5 Real-Time PCR System (Applied Biosystems). ..

    Expressing:

    Article Title: Investigation of the Effect of the Tri-Set Training Method on Plasma mir-1, mir-128a, mir-133, and mir-206 Levels
    Article Snippet: .. To quantify miRNA expression levels, cDNA was synthesized from total RNA using stem-loop primers and the Proto Script II First Strand cDNA Synthesis (NEB) kit. ..

    Synthesized:

    Article Title: Investigation of the Effect of the Tri-Set Training Method on Plasma mir-1, mir-128a, mir-133, and mir-206 Levels
    Article Snippet: .. To quantify miRNA expression levels, cDNA was synthesized from total RNA using stem-loop primers and the Proto Script II First Strand cDNA Synthesis (NEB) kit. ..

    Article Title: The NORAD -Pumilio regulatory axis in the evolution of inclusion body myositis
    Article Snippet: Total RNA was extracted from frozen muscle blocks (20-μm-thick sections, 20 sections per block) using Mini RNeasy Plus Universal Kit (Qiagen, 74134). .. cDNAs were synthesized using ProtoScript II First Strand cDNA Synthesis Kit (New England BioLabs, E6560L). .. Primer sets (synthesized by IDT) for NORAD (human, F: AAGCTGCTCTCAACTCCACC, R: GGACGTATCGCTTCCAGAGG; mouse, F: AGACCTCATCCTGTGAACCT, R: CCTCTTGAA CATGGAACTGCTA), PUM1 (human, F: CTTCAAGACACATGCAACGC, R: TTGTCGGAGTGAAAGGTAAGG; mouse, F: CTTCAAGACACATGCAACGC, R: TTGTCGGAGTGAAAGGTAAGG); and PUM2 (human, F: TCGACCACTACCATTTGTCAG, R: TTCCTCCTCTCCAAGTCCAA; mouse, F: AGAGATATACCGACCACTACCG, R: CAAGCCAAACTCCAGGACAT).

    Article Title: Csi-miR482a protected by food matrix ameliorates dextran sulfate sodium-induced colitis in mice via modulating gut microbes
    Article Snippet: .. The total RNA from the bacteria was extracted using TRNzol Universal reagent (Tiangen, Beijing, China), based on which cDNAs were synthesized using ProtoScript II First Strand cDNA Synthesis kit (New England Biolabs, Ipswich, USA). .. The qPCR was performed on a Bio-rad CFX96 Real-Time PCR Detection System (Hercules, California, USA), using TB Green Premix Ex Taq kit (Takara Bio, Nojihigashi, Japan) and gene specific primers.

    Article Title: A splicing factor-cohesin association links RNA processing to cohesin-dependent genome folding.
    Article Snippet: RNA concentration and purity were assessed using a NanoDrop 2000 spectrophotometer (Thermo). .. Firststrand cDNA was synthesized from 1 μg total RNA using the ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs, #E6560). .. Quantitative PCR was performed using FastStart Universal SYBR Green Master (ROX) (Roche, #04913914001) on a qTOWER3 G touch real-time PCR system (Analytik Jena).

    Bacteria:

    Article Title: Csi-miR482a protected by food matrix ameliorates dextran sulfate sodium-induced colitis in mice via modulating gut microbes
    Article Snippet: .. The total RNA from the bacteria was extracted using TRNzol Universal reagent (Tiangen, Beijing, China), based on which cDNAs were synthesized using ProtoScript II First Strand cDNA Synthesis kit (New England Biolabs, Ipswich, USA). .. The qPCR was performed on a Bio-rad CFX96 Real-Time PCR Detection System (Hercules, California, USA), using TB Green Premix Ex Taq kit (Takara Bio, Nojihigashi, Japan) and gene specific primers.



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