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Servicebio Inc primescript rt pcr kit
Primescript Rt Pcr Kit, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primescript rt pcr kit - by Bioz Stars, 2026-09
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Synthesized:

Article Title: Integrated targeted metabolomics and transcriptomics analysis reveals heterogeneity of subcutaneous and pericardial adipose tissues in Yili horses
Article Snippet: Total RNA was extracted from adipose tissues using an RNA Extraction Kit (Servicebio, Wuhan, China) and quantified using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, Wilmington, DE, USA). .. First-strand cDNA was synthesized from 1 μL total RNA using an RT Kit (Servicebio, Wuhan, China) in a 20 μL reaction containing 5× Reaction Buffer, RT Enzyme Mix, Oligo(dT)18, and random hexamers, incubated at 25 °C for 5 min, 42 °C for 30 min, and 85 °C for 5 s. qPCR assays were performed on a CFX Real-Time PCR System (Bio-Rad, Hercules, CA, USA) using a 15 μL reaction mixture consisting of 7.5 μL 2× SYBR Green Master Mix (None ROX; Servicebio, Wuhan, China), 1.5 μL primer mix (2.5 μM), 2.0 μL of cDNA template, and nuclease-free water. ..

Incubation:

Article Title: Integrated targeted metabolomics and transcriptomics analysis reveals heterogeneity of subcutaneous and pericardial adipose tissues in Yili horses
Article Snippet: Total RNA was extracted from adipose tissues using an RNA Extraction Kit (Servicebio, Wuhan, China) and quantified using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, Wilmington, DE, USA). .. First-strand cDNA was synthesized from 1 μL total RNA using an RT Kit (Servicebio, Wuhan, China) in a 20 μL reaction containing 5× Reaction Buffer, RT Enzyme Mix, Oligo(dT)18, and random hexamers, incubated at 25 °C for 5 min, 42 °C for 30 min, and 85 °C for 5 s. qPCR assays were performed on a CFX Real-Time PCR System (Bio-Rad, Hercules, CA, USA) using a 15 μL reaction mixture consisting of 7.5 μL 2× SYBR Green Master Mix (None ROX; Servicebio, Wuhan, China), 1.5 μL primer mix (2.5 μM), 2.0 μL of cDNA template, and nuclease-free water. ..

Real-time Polymerase Chain Reaction:

Article Title: Integrated targeted metabolomics and transcriptomics analysis reveals heterogeneity of subcutaneous and pericardial adipose tissues in Yili horses
Article Snippet: Total RNA was extracted from adipose tissues using an RNA Extraction Kit (Servicebio, Wuhan, China) and quantified using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, Wilmington, DE, USA). .. First-strand cDNA was synthesized from 1 μL total RNA using an RT Kit (Servicebio, Wuhan, China) in a 20 μL reaction containing 5× Reaction Buffer, RT Enzyme Mix, Oligo(dT)18, and random hexamers, incubated at 25 °C for 5 min, 42 °C for 30 min, and 85 °C for 5 s. qPCR assays were performed on a CFX Real-Time PCR System (Bio-Rad, Hercules, CA, USA) using a 15 μL reaction mixture consisting of 7.5 μL 2× SYBR Green Master Mix (None ROX; Servicebio, Wuhan, China), 1.5 μL primer mix (2.5 μM), 2.0 μL of cDNA template, and nuclease-free water. ..

SYBR Green Assay:

Article Title: Integrated targeted metabolomics and transcriptomics analysis reveals heterogeneity of subcutaneous and pericardial adipose tissues in Yili horses
Article Snippet: Total RNA was extracted from adipose tissues using an RNA Extraction Kit (Servicebio, Wuhan, China) and quantified using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, Wilmington, DE, USA). .. First-strand cDNA was synthesized from 1 μL total RNA using an RT Kit (Servicebio, Wuhan, China) in a 20 μL reaction containing 5× Reaction Buffer, RT Enzyme Mix, Oligo(dT)18, and random hexamers, incubated at 25 °C for 5 min, 42 °C for 30 min, and 85 °C for 5 s. qPCR assays were performed on a CFX Real-Time PCR System (Bio-Rad, Hercules, CA, USA) using a 15 μL reaction mixture consisting of 7.5 μL 2× SYBR Green Master Mix (None ROX; Servicebio, Wuhan, China), 1.5 μL primer mix (2.5 μM), 2.0 μL of cDNA template, and nuclease-free water. ..

Reverse Transcription Polymerase Chain Reaction:

Article Title: Cauloside A interacts with TLR4 to induce JNK/Caspase-3/GSDME-dependent pyroptosis in non-small cell lung cancer cells.
Article Snippet: With non-small cell lung cancer (NSCLC) accounts for the majority of diagnoses, lung cancer continues to be the most common and deadly type of cancer worldwide.. While treatment options have advanced, therapeutic efficacy remains constrained by drug resistance and side effects, highlighting the demand for novel therapeutic agents.. Cauloside A, a natural compound isolated from Fructus Akebiae, has shown anti-tumor potential; however, its precise mechanism of action in NSCLC has remained unclear.

Article Title: ASAP3 activates the NF-κB signaling pathway to promote the progression of esophageal squamous cell carcinoma.
Article Snippet: RT-qPCR Total RNA was extracted from cultured cells using FreeZol reagent (Vazyme, Nanjing, China) according to the manufacturer’s protocol. .. RNA (0.5 μg) was reverse-transcribed into cDNA using a PrimeScript RT-PCR Kit (Servicebio). ..

Reverse Transcription:

Article Title: ASAP3 activates the NF-κB signaling pathway to promote the progression of esophageal squamous cell carcinoma.
Article Snippet: RT-qPCR Total RNA was extracted from cultured cells using FreeZol reagent (Vazyme, Nanjing, China) according to the manufacturer’s protocol. .. RNA (0.5 μg) was reverse-transcribed into cDNA using a PrimeScript RT-PCR Kit (Servicebio). ..

Article Title: Total flavonoids from Abrus cantoniensis alleviate fatty liver hemorrhagic syndrome in laying hens by regulating inflammation, oxidative stress, and cecal metabolites and microbiota
Article Snippet: .. Then, 1 μg of total RNA was reverse-transcribed into cDNA in a 20 μL reaction volume using a commercial reagent kit ( G3337-50, Servicebio Technology Co., Ltd., Wuhan, Hubei, China) according to the manufacturer’s instructions. .. RT-qPCR for target gene was performed by using the CFX 96 Connect Real-Time System (Bio-Rad Laboratories, Inc. Temecula, CA, USA) and the SYBR real-time PCR mix ( G33626-1, Servicebio Technology Co., Ltd., Wuhan, Hubei, China).

Article Title: DCLK1-dependent NF-κB activation mediates p-STAT3-induced osteoarthritis progression.
Article Snippet: Chondrocytes were maintained in dulbecco’s modified eagle’s medium (DMEM) complete medium (Gibco) and maintained in a 5% CO2 humidified incubator at 37◦C.All cellular experimentswere conducted using chondrocytes at passages 1–2. .. Whole cellular RNA was isolated from chondrocytes with TRIzol reagent and reverse-transcribed into cDNA with the PrimeScript RT Reagent Kit (Servicebio). .. Quantitative real-time PCR (RT-qPCR) was then executed on the Roche Light Cycler 480 system with the SYBR Premix Ex Taq Kit (Novoprotein).

Staining:

Article Title: RvD1/FPR2 attenuates cerebral ischemic injury through inhibiting inflammation and oxidative stress.
Article Snippet: The primary antibodies used in this study were as the followings: FPR2 (1:1000; NLS1878, Novus, United States), Bcl-2 (1:1000; sc-7382, Santa Cruz, United States), Bax (1:1000; sc-7480, Santa Cruz, United States), cleaved Caspase-3 (1:1000; 9664S, CST, United States), and β-actin (1:5000; ab8226, Abcam, United States). .. The frozen sections of brains were stained with H&E Assay Kit (G1005, Servicebio, China) and captured under microscope (OLYMPUS, Japan). .. Fluoro-Jade C (FJC) staining was performed according to the manufacturer’s protocol (BSS-TY-100-FJT, Biosensis, United States).

Microscopy:

Article Title: RvD1/FPR2 attenuates cerebral ischemic injury through inhibiting inflammation and oxidative stress.
Article Snippet: The primary antibodies used in this study were as the followings: FPR2 (1:1000; NLS1878, Novus, United States), Bcl-2 (1:1000; sc-7382, Santa Cruz, United States), Bax (1:1000; sc-7480, Santa Cruz, United States), cleaved Caspase-3 (1:1000; 9664S, CST, United States), and β-actin (1:5000; ab8226, Abcam, United States). .. The frozen sections of brains were stained with H&E Assay Kit (G1005, Servicebio, China) and captured under microscope (OLYMPUS, Japan). .. Fluoro-Jade C (FJC) staining was performed according to the manufacturer’s protocol (BSS-TY-100-FJT, Biosensis, United States).

Lactate Assay:

Article Title: Integrating single-cell and bulk transcriptomic analyses to explore key lactylation-related genes in benign prostatic hyperplasia.
Article Snippet: .. Endogenous lactate concentrations were quantified using a Lactate Assay Kit (Servicebio, G4308). ..

Isolation:

Article Title: DCLK1-dependent NF-κB activation mediates p-STAT3-induced osteoarthritis progression.
Article Snippet: Chondrocytes were maintained in dulbecco’s modified eagle’s medium (DMEM) complete medium (Gibco) and maintained in a 5% CO2 humidified incubator at 37◦C.All cellular experimentswere conducted using chondrocytes at passages 1–2. .. Whole cellular RNA was isolated from chondrocytes with TRIzol reagent and reverse-transcribed into cDNA with the PrimeScript RT Reagent Kit (Servicebio). .. Quantitative real-time PCR (RT-qPCR) was then executed on the Roche Light Cycler 480 system with the SYBR Premix Ex Taq Kit (Novoprotein).

Concentration Assay:

Article Title: Lactobacillus johnsonii DM2420 Alleviates Dyslipidemia, Remodels Gut Microbiota, and Modulates the Intestinal CD36/SREBP1 Signaling Axis.
Article Snippet: 1 Department of Pathogen Biology and Microecology, College of Basic Medical Sciences, Dalian Medical University, Dalian, People’s Republic of China 2 Department of Immunology, College of Basic Medical Sciences, Dalian Medical University, Dalian, People’s Republic of China 3 Department of Laboratory Animal Centre, Jinjiang Municipal Hospital (Shanghai sixth People’s Hospital Fujian), Quanzhou, People’s Republic of China Abstract Dyslipidemia is a core pathological manifestation of lipid metabolism disorders, and dysbiosis of gut microbiota plays a key role in its onset and progression.. Building upon our previous findings on the anti-inflammatory and gut microbiota-modulating effects of Lactobacillus johnsonii DM2420, this study further investigated the regulatory effects and underlying mechanisms of this strain in high-fat diet (HFD)-induced dyslipidemia mice.. In vitro, L. johnsonii DM2420 significantly inhibited the accumulation of lipids in 3T3-L1 adipocytes.



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Image Search Results


Construction and screening of phage display library . A PBMCs were isolated from camels, and RNA was extracted as a template for RT-PCR to amplify the gene sequence containing the leader signal sequence before the CH2 region. The amplification yielded two fragments: a 900-bp fragment (VH–CH1–CH2) and a 600-bp fragment (VHH–CH2). Lane M represents the DNA marker (2000–100 bp), and lanes 1–4 represent the amplified fragments. B The 600-bp fragment obtained from the first round of PCR was used as a template for the second round of PCR, which successfully generated the full-length VHH gene (spanning FR1 to FR4) with an approximate size of 400 bp. Lane M represents the DNA marker (1000–200 bp), and lanes 1–4 represent the amplified fragments. C A total of 16 clones were randomly selected from the constructed phage antibody library for identification. D Overall, 92 recombinant phage clones were randomly selected from the third round of panning and added to microplates coated with BRV. HRP-conjugated anti-M13 monoclonal antibody was used to detect the bound phages. M13K07 helper phage and PBS served as the negative control and blank control, respectively. Positive clones were defined as those with a sample-to-negative control ratio (P/N) ≥ 2.1, i.e., clones above the reference line were identified as positive.

Journal: Veterinary Research

Article Title: Potent neutralization and therapeutic efficacy of bovine rotavirus-specific VHH antibodies in infected calves

doi: 10.1186/s13567-026-01765-3

Figure Lengend Snippet: Construction and screening of phage display library . A PBMCs were isolated from camels, and RNA was extracted as a template for RT-PCR to amplify the gene sequence containing the leader signal sequence before the CH2 region. The amplification yielded two fragments: a 900-bp fragment (VH–CH1–CH2) and a 600-bp fragment (VHH–CH2). Lane M represents the DNA marker (2000–100 bp), and lanes 1–4 represent the amplified fragments. B The 600-bp fragment obtained from the first round of PCR was used as a template for the second round of PCR, which successfully generated the full-length VHH gene (spanning FR1 to FR4) with an approximate size of 400 bp. Lane M represents the DNA marker (1000–200 bp), and lanes 1–4 represent the amplified fragments. C A total of 16 clones were randomly selected from the constructed phage antibody library for identification. D Overall, 92 recombinant phage clones were randomly selected from the third round of panning and added to microplates coated with BRV. HRP-conjugated anti-M13 monoclonal antibody was used to detect the bound phages. M13K07 helper phage and PBS served as the negative control and blank control, respectively. Positive clones were defined as those with a sample-to-negative control ratio (P/N) ≥ 2.1, i.e., clones above the reference line were identified as positive.

Article Snippet: The extracted RNA was directly subjected to one-step reverse-transcription polymerase chain reaction (RT-PCR) amplification using the PrimeScriptTM One Step RT-PCR Kit (TaKaRa, Shiga, Japan), which allows reverse transcription and PCR amplification to be conducted in a single reaction.

Techniques: Isolation, Reverse Transcription Polymerase Chain Reaction, Sequencing, Amplification, Marker, Generated, Clone Assay, Construct, Recombinant, Negative Control, Control