primescript rt pcr kit (Servicebio Inc)
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Primescript Rt Pcr Kit, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primescript+rt+pcr+kit/kit+primescript+reagent+rt/pm42277399-101-9-12
Average 86 stars, based on 1 article reviews
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Synthesized:Article Title: Integrated targeted metabolomics and transcriptomics analysis reveals heterogeneity of subcutaneous and pericardial adipose tissues in Yili horses Article Snippet: Total RNA was extracted from adipose tissues using an RNA Extraction Kit (Servicebio, Wuhan, China) and quantified using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, Wilmington, DE, USA). .. First-strand cDNA was synthesized from 1 μL total RNA using an Incubation:Article Title: Integrated targeted metabolomics and transcriptomics analysis reveals heterogeneity of subcutaneous and pericardial adipose tissues in Yili horses Article Snippet: Total RNA was extracted from adipose tissues using an RNA Extraction Kit (Servicebio, Wuhan, China) and quantified using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, Wilmington, DE, USA). .. First-strand cDNA was synthesized from 1 μL total RNA using an Real-time Polymerase Chain Reaction:Article Title: Integrated targeted metabolomics and transcriptomics analysis reveals heterogeneity of subcutaneous and pericardial adipose tissues in Yili horses Article Snippet: Total RNA was extracted from adipose tissues using an RNA Extraction Kit (Servicebio, Wuhan, China) and quantified using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, Wilmington, DE, USA). .. First-strand cDNA was synthesized from 1 μL total RNA using an SYBR Green Assay:Article Title: Integrated targeted metabolomics and transcriptomics analysis reveals heterogeneity of subcutaneous and pericardial adipose tissues in Yili horses Article Snippet: Total RNA was extracted from adipose tissues using an RNA Extraction Kit (Servicebio, Wuhan, China) and quantified using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, Wilmington, DE, USA). .. First-strand cDNA was synthesized from 1 μL total RNA using an Reverse Transcription Polymerase Chain Reaction:Article Title: Cauloside A interacts with TLR4 to induce JNK/Caspase-3/GSDME-dependent pyroptosis in non-small cell lung cancer cells. Article Snippet: With non-small cell lung cancer (NSCLC) accounts for the majority of diagnoses, lung cancer continues to be the most common and deadly type of cancer worldwide.. While treatment options have advanced, therapeutic efficacy remains constrained by drug resistance and side effects, highlighting the demand for novel therapeutic agents.. Cauloside A, a natural compound isolated from Fructus Akebiae, has shown anti-tumor potential; however, its precise mechanism of action in NSCLC has remained unclear. Article Title: ASAP3 activates the NF-κB signaling pathway to promote the progression of esophageal squamous cell carcinoma. Article Snippet: RT-qPCR Total RNA was extracted from cultured cells using FreeZol reagent (Vazyme, Nanjing, China) according to the manufacturer’s protocol. .. RNA (0.5 μg) was reverse-transcribed into cDNA using a Reverse Transcription:Article Title: ASAP3 activates the NF-κB signaling pathway to promote the progression of esophageal squamous cell carcinoma. Article Snippet: RT-qPCR Total RNA was extracted from cultured cells using FreeZol reagent (Vazyme, Nanjing, China) according to the manufacturer’s protocol. .. RNA (0.5 μg) was reverse-transcribed into cDNA using a Article Title: Total flavonoids from Abrus cantoniensis alleviate fatty liver hemorrhagic syndrome in laying hens by regulating inflammation, oxidative stress, and cecal metabolites and microbiota Article Snippet: .. Then, 1 μg of total RNA was reverse-transcribed into cDNA in a 20 μL reaction volume using a commercial Article Title: DCLK1-dependent NF-κB activation mediates p-STAT3-induced osteoarthritis progression. Article Snippet: Chondrocytes were maintained in dulbecco’s modified eagle’s medium (DMEM) complete medium (Gibco) and maintained in a 5% CO2 humidified incubator at 37◦C.All cellular experimentswere conducted using chondrocytes at passages 1–2. .. Whole cellular RNA was isolated from chondrocytes with TRIzol reagent and reverse-transcribed into cDNA with the Staining:Article Title: RvD1/FPR2 attenuates cerebral ischemic injury through inhibiting inflammation and oxidative stress. Article Snippet: The primary antibodies used in this study were as the followings: FPR2 (1:1000; NLS1878, Novus, United States), Bcl-2 (1:1000; sc-7382, Santa Cruz, United States), Bax (1:1000; sc-7480, Santa Cruz, United States), cleaved Caspase-3 (1:1000; 9664S, CST, United States), and β-actin (1:5000; ab8226, Abcam, United States). .. The frozen sections of brains were stained with Microscopy:Article Title: RvD1/FPR2 attenuates cerebral ischemic injury through inhibiting inflammation and oxidative stress. Article Snippet: The primary antibodies used in this study were as the followings: FPR2 (1:1000; NLS1878, Novus, United States), Bcl-2 (1:1000; sc-7382, Santa Cruz, United States), Bax (1:1000; sc-7480, Santa Cruz, United States), cleaved Caspase-3 (1:1000; 9664S, CST, United States), and β-actin (1:5000; ab8226, Abcam, United States). .. The frozen sections of brains were stained with Lactate Assay:Article Title: Integrating single-cell and bulk transcriptomic analyses to explore key lactylation-related genes in benign prostatic hyperplasia. Article Snippet: .. Endogenous lactate concentrations were quantified using a Isolation:Article Title: DCLK1-dependent NF-κB activation mediates p-STAT3-induced osteoarthritis progression. Article Snippet: Chondrocytes were maintained in dulbecco’s modified eagle’s medium (DMEM) complete medium (Gibco) and maintained in a 5% CO2 humidified incubator at 37◦C.All cellular experimentswere conducted using chondrocytes at passages 1–2. .. Whole cellular RNA was isolated from chondrocytes with TRIzol reagent and reverse-transcribed into cDNA with the Concentration Assay:Article Title: Lactobacillus johnsonii DM2420 Alleviates Dyslipidemia, Remodels Gut Microbiota, and Modulates the Intestinal CD36/SREBP1 Signaling Axis. Article Snippet: 1 Department of Pathogen Biology and Microecology, College of Basic Medical Sciences, Dalian Medical University, Dalian, People’s Republic of China 2 Department of Immunology, College of Basic Medical Sciences, Dalian Medical University, Dalian, People’s Republic of China 3 Department of Laboratory Animal Centre, Jinjiang Municipal Hospital (Shanghai sixth People’s Hospital Fujian), Quanzhou, People’s Republic of China Abstract Dyslipidemia is a core pathological manifestation of lipid metabolism disorders, and dysbiosis of gut microbiota plays a key role in its onset and progression.. Building upon our previous findings on the anti-inflammatory and gut microbiota-modulating effects of Lactobacillus johnsonii DM2420, this study further investigated the regulatory effects and underlying mechanisms of this strain in high-fat diet (HFD)-induced dyslipidemia mice.. In vitro, L. johnsonii DM2420 significantly inhibited the accumulation of lipids in 3T3-L1 adipocytes. |
