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step primescript rt pcr kit  (TaKaRa)


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    TaKaRa step primescript rt pcr kit
    Step Primescript Rt Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 10918 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescript+rt+pcr+kit/PrimeScript+RT-PCR+Kit/pm42139316-359-8-12
    Average 98 stars, based on 10918 article reviews
    step primescript rt pcr kit - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: KRAS (G12D)-selective inhibitor MRTX1133 suppresses proliferation and differentially modulates chemosensitivity in ovarian mucinous carcinoma
    Article Snippet: Cellular mRNA was extracted using the RNeasy Mini Kit (Qiagen, Inc.) according to the manufacturer's instructions. .. Reverse transcription and subsequent qPCR were performed using the One Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time; cat. no. RR066A; Takara Bio, Inc.), which includes TB Green as the fluorophore, according to the manufacturer's protocol. .. RT-qPCR was performed using the Thermal Cycler Dice Real Time System II (Takara Bio, Inc.) following the manufacturer's instructions.

    Article Title: DHT-Induced lncRNA AC092718.4 Promotes Prostate Cancer Cell Proliferation via ceRNA Mechanism
    Article Snippet: Total RNA was isolated using the RNeasy®Plus mini Kit (QIAGEN, Dusseldorf, Germany) according to the manufacturer’s protocol. .. Reverse transcription of total RNA was carried out using the PrimeScriptTM RT-PCR Kit (Takara, Tokyo, Japan), while reverse transcription of miRNAs was carried out with the miRNA 1st Strand cDNA Synthesis Kit (Vazyme, Nanjing, China). qPCR was conducted by using TB Green® Premix Ex TaqTM II (Tli RNaseH Plus) (Takara, Tokyo, Japan). ..

    Article Title: Potent neutralization and therapeutic efficacy of bovine rotavirus-specific VHH antibodies in infected calves
    Article Snippet: Total RNA was extracted from PBMCs using TRIzol reagent (Ambion, Austin, TX, USA) according to the manufacturer’s instructions. .. The extracted RNA was directly subjected to one-step reverse-transcription polymerase chain reaction (RT-PCR) amplification using the PrimeScriptTM One Step RT-PCR Kit (TaKaRa, Shiga, Japan), which allows reverse transcription and PCR amplification to be conducted in a single reaction. ..

    Article Title: MYC promotes myocardial fibrosis via METTL1-mediated m7G modification of HILPDA.
    Article Snippet: Quantitative real-time PCR (qRT-PCR) Total RNA was extracted from cardiac tissues and MCFs using TRIzol reagent (Servicebio), and its concentration and purity were AR TIC LE IN PR ES S determined using a spectrophotometer. .. Subsequently, 1 μg of total RNA from each sample was reverse-transcribed into cDNA using the PrimeScriptTM RT Kit (Takara, Japan) according to the manufacturer's instructions. qRT-PCR was then performed in triplicate using TB Green® Premix Ex TaqTM II Kit (Takara) on a realtime PCR detection system. ..

    Real-time Polymerase Chain Reaction:

    Article Title: KRAS (G12D)-selective inhibitor MRTX1133 suppresses proliferation and differentially modulates chemosensitivity in ovarian mucinous carcinoma
    Article Snippet: Cellular mRNA was extracted using the RNeasy Mini Kit (Qiagen, Inc.) according to the manufacturer's instructions. .. Reverse transcription and subsequent qPCR were performed using the One Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time; cat. no. RR066A; Takara Bio, Inc.), which includes TB Green as the fluorophore, according to the manufacturer's protocol. .. RT-qPCR was performed using the Thermal Cycler Dice Real Time System II (Takara Bio, Inc.) following the manufacturer's instructions.

    Article Title: Food Structure Modulation of Antioxidant Bioaccessibility and Gut Epithelial Protection: The Case of Bread and Pasta Made With Pigmented Wheat
    Article Snippet: Total RNA was extracted from the Caco‐2 pellet using the Total RNA Purification Plus Kit (Norgen Biotek, Thorold, ON, Canada), according to the manufacturer's instructions and quantified (NanoDrop, Thermo Fisher Scientific, Italy). .. RNA (1 μg) was retrotranscribed to cDNA using the PrimeScript RT‐PCR Kit (Takara Bio, Göteborg, Sweden), and quantitative real‐time PCR (Biorad CFX96) was used to perform the gene expression analysis using TB Green Premix Ex Taq (Takara Bio, Göteborg, Sweden). ..

    Article Title: DHT-Induced lncRNA AC092718.4 Promotes Prostate Cancer Cell Proliferation via ceRNA Mechanism
    Article Snippet: Total RNA was isolated using the RNeasy®Plus mini Kit (QIAGEN, Dusseldorf, Germany) according to the manufacturer’s protocol. .. Reverse transcription of total RNA was carried out using the PrimeScriptTM RT-PCR Kit (Takara, Tokyo, Japan), while reverse transcription of miRNAs was carried out with the miRNA 1st Strand cDNA Synthesis Kit (Vazyme, Nanjing, China). qPCR was conducted by using TB Green® Premix Ex TaqTM II (Tli RNaseH Plus) (Takara, Tokyo, Japan). ..

    Article Title: Molecular basis of copper homeostasis in Trichoderma asperellum Ts93: roles of a peroxidase and Ctr-family transporters.
    Article Snippet: agents, fungi exhibit superior metal chelation capabilities for the removal of heavy metals (Rosa et al. 2024; Vaksmaa et al. 2023).. Consequently, investigating the copper tolerance mechanisms in fungi is essential for developing effective bioremediation strategies.. The genus Trichoderma comprises filamentous fungi renowned for their ecological versatility and biotechnological applications, including their ability to tolerate elevated copper levels (Ramírez-Valdespino & Orrantia-Borunda, 2021).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: KRAS (G12D)-selective inhibitor MRTX1133 suppresses proliferation and differentially modulates chemosensitivity in ovarian mucinous carcinoma
    Article Snippet: Cellular mRNA was extracted using the RNeasy Mini Kit (Qiagen, Inc.) according to the manufacturer's instructions. .. Reverse transcription and subsequent qPCR were performed using the One Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time; cat. no. RR066A; Takara Bio, Inc.), which includes TB Green as the fluorophore, according to the manufacturer's protocol. .. RT-qPCR was performed using the Thermal Cycler Dice Real Time System II (Takara Bio, Inc.) following the manufacturer's instructions.

    Article Title: DHT-Induced lncRNA AC092718.4 Promotes Prostate Cancer Cell Proliferation via ceRNA Mechanism
    Article Snippet: Total RNA was isolated using the RNeasy®Plus mini Kit (QIAGEN, Dusseldorf, Germany) according to the manufacturer’s protocol. .. Reverse transcription of total RNA was carried out using the PrimeScriptTM RT-PCR Kit (Takara, Tokyo, Japan), while reverse transcription of miRNAs was carried out with the miRNA 1st Strand cDNA Synthesis Kit (Vazyme, Nanjing, China). qPCR was conducted by using TB Green® Premix Ex TaqTM II (Tli RNaseH Plus) (Takara, Tokyo, Japan). ..

    Article Title: CD36/ANXA1/TLR4/NF-κB Axis Orchestrates Lipid Metabolism, Chronic Inflammation, and Insulin Resistance in Obese Children.
    Article Snippet: Background: Childhood obesity is tightly linked to dyslipidemia, chronic low-grade inflammation, and insulin resistance (IR).. CD36 has been implicated in metabolic disease, yet its pediatric mechanisms remain unclear.. This study aims to investigate the CD36/ANXA1/TLR4/NF-κB axis as a potential therapeutic target for lipid metabolism and IR in childhood obesity.

    Article Title: Characterization of the virus-host RNA-RNA interactome across important human pathogenic RNA viruses.
    Article Snippet: .. SYBR Green RT-qPCR was performed using the One step primescript RT-PCR kit (Takara) according to manufacturer’s instructions. ..

    Article Title: Molecular basis of copper homeostasis in Trichoderma asperellum Ts93: roles of a peroxidase and Ctr-family transporters.
    Article Snippet: agents, fungi exhibit superior metal chelation capabilities for the removal of heavy metals (Rosa et al. 2024; Vaksmaa et al. 2023).. Consequently, investigating the copper tolerance mechanisms in fungi is essential for developing effective bioremediation strategies.. The genus Trichoderma comprises filamentous fungi renowned for their ecological versatility and biotechnological applications, including their ability to tolerate elevated copper levels (Ramírez-Valdespino & Orrantia-Borunda, 2021).

    Gene Expression:

    Article Title: Food Structure Modulation of Antioxidant Bioaccessibility and Gut Epithelial Protection: The Case of Bread and Pasta Made With Pigmented Wheat
    Article Snippet: Total RNA was extracted from the Caco‐2 pellet using the Total RNA Purification Plus Kit (Norgen Biotek, Thorold, ON, Canada), according to the manufacturer's instructions and quantified (NanoDrop, Thermo Fisher Scientific, Italy). .. RNA (1 μg) was retrotranscribed to cDNA using the PrimeScript RT‐PCR Kit (Takara Bio, Göteborg, Sweden), and quantitative real‐time PCR (Biorad CFX96) was used to perform the gene expression analysis using TB Green Premix Ex Taq (Takara Bio, Göteborg, Sweden). ..

    cDNA Synthesis:

    Article Title: DHT-Induced lncRNA AC092718.4 Promotes Prostate Cancer Cell Proliferation via ceRNA Mechanism
    Article Snippet: Total RNA was isolated using the RNeasy®Plus mini Kit (QIAGEN, Dusseldorf, Germany) according to the manufacturer’s protocol. .. Reverse transcription of total RNA was carried out using the PrimeScriptTM RT-PCR Kit (Takara, Tokyo, Japan), while reverse transcription of miRNAs was carried out with the miRNA 1st Strand cDNA Synthesis Kit (Vazyme, Nanjing, China). qPCR was conducted by using TB Green® Premix Ex TaqTM II (Tli RNaseH Plus) (Takara, Tokyo, Japan). ..

    Article Title: Molecular basis of copper homeostasis in Trichoderma asperellum Ts93: roles of a peroxidase and Ctr-family transporters.
    Article Snippet: agents, fungi exhibit superior metal chelation capabilities for the removal of heavy metals (Rosa et al. 2024; Vaksmaa et al. 2023).. Consequently, investigating the copper tolerance mechanisms in fungi is essential for developing effective bioremediation strategies.. The genus Trichoderma comprises filamentous fungi renowned for their ecological versatility and biotechnological applications, including their ability to tolerate elevated copper levels (Ramírez-Valdespino & Orrantia-Borunda, 2021).

    Polymerase Chain Reaction:

    Article Title: Potent neutralization and therapeutic efficacy of bovine rotavirus-specific VHH antibodies in infected calves
    Article Snippet: Total RNA was extracted from PBMCs using TRIzol reagent (Ambion, Austin, TX, USA) according to the manufacturer’s instructions. .. The extracted RNA was directly subjected to one-step reverse-transcription polymerase chain reaction (RT-PCR) amplification using the PrimeScriptTM One Step RT-PCR Kit (TaKaRa, Shiga, Japan), which allows reverse transcription and PCR amplification to be conducted in a single reaction. ..

    Article Title: MYC promotes myocardial fibrosis via METTL1-mediated m7G modification of HILPDA.
    Article Snippet: Quantitative real-time PCR (qRT-PCR) Total RNA was extracted from cardiac tissues and MCFs using TRIzol reagent (Servicebio), and its concentration and purity were AR TIC LE IN PR ES S determined using a spectrophotometer. .. Subsequently, 1 μg of total RNA from each sample was reverse-transcribed into cDNA using the PrimeScriptTM RT Kit (Takara, Japan) according to the manufacturer's instructions. qRT-PCR was then performed in triplicate using TB Green® Premix Ex TaqTM II Kit (Takara) on a realtime PCR detection system. ..

    Amplification:

    Article Title: Potent neutralization and therapeutic efficacy of bovine rotavirus-specific VHH antibodies in infected calves
    Article Snippet: Total RNA was extracted from PBMCs using TRIzol reagent (Ambion, Austin, TX, USA) according to the manufacturer’s instructions. .. The extracted RNA was directly subjected to one-step reverse-transcription polymerase chain reaction (RT-PCR) amplification using the PrimeScriptTM One Step RT-PCR Kit (TaKaRa, Shiga, Japan), which allows reverse transcription and PCR amplification to be conducted in a single reaction. ..

    One Step RT-PCR:

    Article Title: Potent neutralization and therapeutic efficacy of bovine rotavirus-specific VHH antibodies in infected calves
    Article Snippet: Total RNA was extracted from PBMCs using TRIzol reagent (Ambion, Austin, TX, USA) according to the manufacturer’s instructions. .. The extracted RNA was directly subjected to one-step reverse-transcription polymerase chain reaction (RT-PCR) amplification using the PrimeScriptTM One Step RT-PCR Kit (TaKaRa, Shiga, Japan), which allows reverse transcription and PCR amplification to be conducted in a single reaction. ..

    Quantitative RT-PCR:

    Article Title: MYC promotes myocardial fibrosis via METTL1-mediated m7G modification of HILPDA.
    Article Snippet: Quantitative real-time PCR (qRT-PCR) Total RNA was extracted from cardiac tissues and MCFs using TRIzol reagent (Servicebio), and its concentration and purity were AR TIC LE IN PR ES S determined using a spectrophotometer. .. Subsequently, 1 μg of total RNA from each sample was reverse-transcribed into cDNA using the PrimeScriptTM RT Kit (Takara, Japan) according to the manufacturer's instructions. qRT-PCR was then performed in triplicate using TB Green® Premix Ex TaqTM II Kit (Takara) on a realtime PCR detection system. ..

    Article Title: Characterization of the virus-host RNA-RNA interactome across important human pathogenic RNA viruses.
    Article Snippet: .. SYBR Green RT-qPCR was performed using the One step primescript RT-PCR kit (Takara) according to manufacturer’s instructions. ..

    Article Title: Molecular basis of copper homeostasis in Trichoderma asperellum Ts93: roles of a peroxidase and Ctr-family transporters.
    Article Snippet: agents, fungi exhibit superior metal chelation capabilities for the removal of heavy metals (Rosa et al. 2024; Vaksmaa et al. 2023).. Consequently, investigating the copper tolerance mechanisms in fungi is essential for developing effective bioremediation strategies.. The genus Trichoderma comprises filamentous fungi renowned for their ecological versatility and biotechnological applications, including their ability to tolerate elevated copper levels (Ramírez-Valdespino & Orrantia-Borunda, 2021).

    Synthesized:

    Article Title: CD36/ANXA1/TLR4/NF-κB Axis Orchestrates Lipid Metabolism, Chronic Inflammation, and Insulin Resistance in Obese Children.
    Article Snippet: Background: Childhood obesity is tightly linked to dyslipidemia, chronic low-grade inflammation, and insulin resistance (IR).. CD36 has been implicated in metabolic disease, yet its pediatric mechanisms remain unclear.. This study aims to investigate the CD36/ANXA1/TLR4/NF-κB axis as a potential therapeutic target for lipid metabolism and IR in childhood obesity.

    Article Title: Molecular basis of copper homeostasis in Trichoderma asperellum Ts93: roles of a peroxidase and Ctr-family transporters.
    Article Snippet: agents, fungi exhibit superior metal chelation capabilities for the removal of heavy metals (Rosa et al. 2024; Vaksmaa et al. 2023).. Consequently, investigating the copper tolerance mechanisms in fungi is essential for developing effective bioremediation strategies.. The genus Trichoderma comprises filamentous fungi renowned for their ecological versatility and biotechnological applications, including their ability to tolerate elevated copper levels (Ramírez-Valdespino & Orrantia-Borunda, 2021).

    SYBR Green Assay:

    Article Title: Characterization of the virus-host RNA-RNA interactome across important human pathogenic RNA viruses.
    Article Snippet: .. SYBR Green RT-qPCR was performed using the One step primescript RT-PCR kit (Takara) according to manufacturer’s instructions. ..

    RNA Sequencing:

    Article Title: Molecular basis of copper homeostasis in Trichoderma asperellum Ts93: roles of a peroxidase and Ctr-family transporters.
    Article Snippet: agents, fungi exhibit superior metal chelation capabilities for the removal of heavy metals (Rosa et al. 2024; Vaksmaa et al. 2023).. Consequently, investigating the copper tolerance mechanisms in fungi is essential for developing effective bioremediation strategies.. The genus Trichoderma comprises filamentous fungi renowned for their ecological versatility and biotechnological applications, including their ability to tolerate elevated copper levels (Ramírez-Valdespino & Orrantia-Borunda, 2021).



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    Construction and screening of phage display library . A PBMCs were isolated from camels, and RNA was extracted as a template <t>for</t> <t>RT-PCR</t> to amplify the gene sequence containing the leader signal sequence before the CH2 region. The amplification yielded two fragments: a 900-bp fragment (VH–CH1–CH2) and a 600-bp fragment (VHH–CH2). Lane M represents the DNA marker (2000–100 bp), and lanes 1–4 represent the amplified fragments. B The 600-bp fragment obtained from the first round of PCR was used as a template for the second round of PCR, which successfully generated the full-length VHH gene (spanning FR1 to FR4) with an approximate size of 400 bp. Lane M represents the DNA marker (1000–200 bp), and lanes 1–4 represent the amplified fragments. C A total of 16 clones were randomly selected from the constructed phage antibody library for identification. D Overall, 92 recombinant phage clones were randomly selected from the third round of panning and added to microplates coated with BRV. HRP-conjugated anti-M13 monoclonal antibody was used to detect the bound phages. M13K07 helper phage and PBS served as the negative control and blank control, respectively. Positive clones were defined as those with a sample-to-negative control ratio (P/N) ≥ 2.1, i.e., clones above the reference line were identified as positive.
    Primescript Rt Pcr Master Mix Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Construction and screening of phage display library . A PBMCs were isolated from camels, and RNA was extracted as a template for RT-PCR to amplify the gene sequence containing the leader signal sequence before the CH2 region. The amplification yielded two fragments: a 900-bp fragment (VH–CH1–CH2) and a 600-bp fragment (VHH–CH2). Lane M represents the DNA marker (2000–100 bp), and lanes 1–4 represent the amplified fragments. B The 600-bp fragment obtained from the first round of PCR was used as a template for the second round of PCR, which successfully generated the full-length VHH gene (spanning FR1 to FR4) with an approximate size of 400 bp. Lane M represents the DNA marker (1000–200 bp), and lanes 1–4 represent the amplified fragments. C A total of 16 clones were randomly selected from the constructed phage antibody library for identification. D Overall, 92 recombinant phage clones were randomly selected from the third round of panning and added to microplates coated with BRV. HRP-conjugated anti-M13 monoclonal antibody was used to detect the bound phages. M13K07 helper phage and PBS served as the negative control and blank control, respectively. Positive clones were defined as those with a sample-to-negative control ratio (P/N) ≥ 2.1, i.e., clones above the reference line were identified as positive.

    Journal: Veterinary Research

    Article Title: Potent neutralization and therapeutic efficacy of bovine rotavirus-specific VHH antibodies in infected calves

    doi: 10.1186/s13567-026-01765-3

    Figure Lengend Snippet: Construction and screening of phage display library . A PBMCs were isolated from camels, and RNA was extracted as a template for RT-PCR to amplify the gene sequence containing the leader signal sequence before the CH2 region. The amplification yielded two fragments: a 900-bp fragment (VH–CH1–CH2) and a 600-bp fragment (VHH–CH2). Lane M represents the DNA marker (2000–100 bp), and lanes 1–4 represent the amplified fragments. B The 600-bp fragment obtained from the first round of PCR was used as a template for the second round of PCR, which successfully generated the full-length VHH gene (spanning FR1 to FR4) with an approximate size of 400 bp. Lane M represents the DNA marker (1000–200 bp), and lanes 1–4 represent the amplified fragments. C A total of 16 clones were randomly selected from the constructed phage antibody library for identification. D Overall, 92 recombinant phage clones were randomly selected from the third round of panning and added to microplates coated with BRV. HRP-conjugated anti-M13 monoclonal antibody was used to detect the bound phages. M13K07 helper phage and PBS served as the negative control and blank control, respectively. Positive clones were defined as those with a sample-to-negative control ratio (P/N) ≥ 2.1, i.e., clones above the reference line were identified as positive.

    Article Snippet: The extracted RNA was directly subjected to one-step reverse-transcription polymerase chain reaction (RT-PCR) amplification using the PrimeScriptTM One Step RT-PCR Kit (TaKaRa, Shiga, Japan), which allows reverse transcription and PCR amplification to be conducted in a single reaction.

    Techniques: Isolation, Reverse Transcription Polymerase Chain Reaction, Sequencing, Amplification, Marker, Generated, Clone Assay, Construct, Recombinant, Negative Control, Control