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primescript rt pcr master mix kit  (TaKaRa)


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    TaKaRa primescript rt pcr master mix kit
    Primescript Rt Pcr Master Mix Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 26665 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescript+rt+pcr+kit/PrimeScript+RT+Master+Mix/pm42129801-331-18-23
    Average 99 stars, based on 26665 article reviews
    primescript rt pcr master mix kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    cDNA Synthesis:

    Article Title: Hematopoietic progenitor kinase 1 inhibitor BGB-15025 induces apoptosis in acute myeloid leukemia cells through the cell cycle pathway and mitogen-activated protein kinase/extracellular signal-regulated kinase pathway signaling axis
    Article Snippet: Monocyte-derived total RNA was extracted using the TransZol Up Plus RNA kit (TransGen Biotech) following manufacturer-recommended protocols. .. First-strand cDNA synthesis was performed with the PrimeScript RT Master Mix (RR037Q; Takara Bio, Shiga, Japan) through reverse transcription of 1 μg total RNA. .. Quantitative PCR amplification was conducted in triplicate reactions (10 μl total volume containing 1 μl cDNA template) using TB Green Premix Ex Taq II (#639676; Takara Bio) on a 7500 Fast Real-Time PCR System (Applied Biosystems, Singapore, Singapore).

    Reverse Transcription:

    Article Title: Hematopoietic progenitor kinase 1 inhibitor BGB-15025 induces apoptosis in acute myeloid leukemia cells through the cell cycle pathway and mitogen-activated protein kinase/extracellular signal-regulated kinase pathway signaling axis
    Article Snippet: Monocyte-derived total RNA was extracted using the TransZol Up Plus RNA kit (TransGen Biotech) following manufacturer-recommended protocols. .. First-strand cDNA synthesis was performed with the PrimeScript RT Master Mix (RR037Q; Takara Bio, Shiga, Japan) through reverse transcription of 1 μg total RNA. .. Quantitative PCR amplification was conducted in triplicate reactions (10 μl total volume containing 1 μl cDNA template) using TB Green Premix Ex Taq II (#639676; Takara Bio) on a 7500 Fast Real-Time PCR System (Applied Biosystems, Singapore, Singapore).

    Article Title: Extracellular biogenic nanoscale mitochondria reprogram the wound microenvironment via ROS scavenging independent of cellular uptake
    Article Snippet: Total RNA was extracted from cultured cells and peri-wound tissue samples using the RNeasy Mini Kit (Qiagen, Cat# 74014), following the manufacturer's protocol. .. Reverse transcription was performed using the PrimeScriptTM RT Master Mix (Takara, Cat# RR036A). .. Quantitative real-time PCR (qPCR) was conducted using SYBR Green chemistry on an ABI 7500 Real-Time PCR System (Applied Biosystems).

    Article Title: Inhalable PD-L1-engineered hybrid cellular vesicles suppress excessive neutrophil activation and restore mitochondrial homeostasis to alleviate ischemia–reperfusion lung injury and pneumonia
    Article Snippet: .. Total RNA was extracted using an RNA extraction kit (RC112, Vazyme, China) and reverse-transcribed into cDNA with a PCR kit (RR036A, Takara, Japan). .. Amplification reactions were carried out using the SYBR Green method on a real-time PCR system (Bio-Rad, USA).

    Synthesized:

    Article Title: AdMSC spheroids encapsulating antioxidant hybrid protein carrier for irradiation-damaged salivary gland repair
    Article Snippet: Total RNA was isolated from SG tissue using TRIzolTM LS Reagent (Invitrogen, Cat. No. 10296010) according to the manufacturer's protocol. .. Complementary DNA (cDNA) was synthesized from 500 to 1000 ng of total RNA using PrimeScript RT Master Mix (TaKaRa, Cat. No. RR036A), and quantitative PCR was performed with TB Green® Premix Ex TaqTM (TaKaRa, Cat. No. RR420A) on a real-time PCR system using gene-specific primers for VEGF, HGF, CD31, and vWF, with GAPDH as the internal reference gene. ..

    Real-time Polymerase Chain Reaction:

    Article Title: AdMSC spheroids encapsulating antioxidant hybrid protein carrier for irradiation-damaged salivary gland repair
    Article Snippet: Total RNA was isolated from SG tissue using TRIzolTM LS Reagent (Invitrogen, Cat. No. 10296010) according to the manufacturer's protocol. .. Complementary DNA (cDNA) was synthesized from 500 to 1000 ng of total RNA using PrimeScript RT Master Mix (TaKaRa, Cat. No. RR036A), and quantitative PCR was performed with TB Green® Premix Ex TaqTM (TaKaRa, Cat. No. RR420A) on a real-time PCR system using gene-specific primers for VEGF, HGF, CD31, and vWF, with GAPDH as the internal reference gene. ..

    Article Title: Adaptable sliding hydrogels enable pericellular pocket formation while enhancing MSC chondrogenesis and survival in 3D
    Article Snippet: Hydrogels were transferred to TRIzol (Thermo Fisher Scientific), crushed, and homogenized for phenol-chloroform RNA extraction and reverse transcript PCR (RT-PCR). .. Following preparation of cDNA using Primescript RT Master Mix (Takara Bio) in a MiniAmp Plus Thermal Cycler, q-PCR was conducted using Power SYBR Green PCR Master Mix (Applied Biosciences) and an Applied Biosystems QuantStudio 6 Pro Real-Time PCR system. ..

    Article Title: Intelligent-responsive hydrogel synergistically mediates immune remodel-antibacterial-angiogenesis cascade for diabetic foot ulcer repair
    Article Snippet: .. Methyl alcohol, Dimethyl sulfoxide (DMSO; General-Reagent, Titan, Shanghai, China); Deferoxamine mesylate (DFO; MCE, New Jersey, USA); Zn(NO 3 ) 2 ·6H 2 O, Mn(NO 3 ) 2 ·4H 2 O, 2-methylimidazole (Aladdin, Shanghai, China); UCMSC-exo (Langfang Kangbao Huitai Biotechnology, Langfang, China); Chitosan (CS; Deacetylation degree ≥95%, Macklin, Shanghai, China), 4-carboxyphenylboronic acid; polyvinyl alcohol (4-CPBA, PVA; Aladdin, Shanghai, China); N-(3-Dimethylaminopropyl)-N′-ethyl carbodiimide hydrochloride (EDC; Aladdin, Shanghai, China); N-Hydroxysuccinimide (NHS; Aladdin, Shanghai, China); PrimeScript RT Master Mix (Takara, Beijing, China); qPCR SYBR Green Master Mix (Yeasen, Shanghai, China); CD9 antibody (Selleck, USA); TSG101 antibody (Abmart, Shanghai, China); CD86 flow antibody and CD206 flow antibody (BioLegend, USA); IL-6, CD206, HIF-1α, CK14, Ki67, CD31, VEGF and α-SMA (AiFang biological, China). ..

    RNA Extraction:

    Article Title: Inhalable PD-L1-engineered hybrid cellular vesicles suppress excessive neutrophil activation and restore mitochondrial homeostasis to alleviate ischemia–reperfusion lung injury and pneumonia
    Article Snippet: .. Total RNA was extracted using an RNA extraction kit (RC112, Vazyme, China) and reverse-transcribed into cDNA with a PCR kit (RR036A, Takara, Japan). .. Amplification reactions were carried out using the SYBR Green method on a real-time PCR system (Bio-Rad, USA).

    Polymerase Chain Reaction:

    Article Title: Inhalable PD-L1-engineered hybrid cellular vesicles suppress excessive neutrophil activation and restore mitochondrial homeostasis to alleviate ischemia–reperfusion lung injury and pneumonia
    Article Snippet: .. Total RNA was extracted using an RNA extraction kit (RC112, Vazyme, China) and reverse-transcribed into cDNA with a PCR kit (RR036A, Takara, Japan). .. Amplification reactions were carried out using the SYBR Green method on a real-time PCR system (Bio-Rad, USA).

    Article Title: Adaptable sliding hydrogels enable pericellular pocket formation while enhancing MSC chondrogenesis and survival in 3D
    Article Snippet: Hydrogels were transferred to TRIzol (Thermo Fisher Scientific), crushed, and homogenized for phenol-chloroform RNA extraction and reverse transcript PCR (RT-PCR). .. Following preparation of cDNA using Primescript RT Master Mix (Takara Bio) in a MiniAmp Plus Thermal Cycler, q-PCR was conducted using Power SYBR Green PCR Master Mix (Applied Biosciences) and an Applied Biosystems QuantStudio 6 Pro Real-Time PCR system. ..

    other:

    Article Title: Interplay between nuclear survivin and the PRC2 complex and its impact on H3K27me3-directed transcriptional repression
    Article Snippet: The samples were treated with DNase I, using the RNase free DNase kit (Qiagen1023460) and RNA concentration determined with a Nanodrop 2000 (Thermo Fisher Scientific).

    SYBR Green Assay:

    Article Title: Adaptable sliding hydrogels enable pericellular pocket formation while enhancing MSC chondrogenesis and survival in 3D
    Article Snippet: Hydrogels were transferred to TRIzol (Thermo Fisher Scientific), crushed, and homogenized for phenol-chloroform RNA extraction and reverse transcript PCR (RT-PCR). .. Following preparation of cDNA using Primescript RT Master Mix (Takara Bio) in a MiniAmp Plus Thermal Cycler, q-PCR was conducted using Power SYBR Green PCR Master Mix (Applied Biosciences) and an Applied Biosystems QuantStudio 6 Pro Real-Time PCR system. ..

    Article Title: Intelligent-responsive hydrogel synergistically mediates immune remodel-antibacterial-angiogenesis cascade for diabetic foot ulcer repair
    Article Snippet: .. Methyl alcohol, Dimethyl sulfoxide (DMSO; General-Reagent, Titan, Shanghai, China); Deferoxamine mesylate (DFO; MCE, New Jersey, USA); Zn(NO 3 ) 2 ·6H 2 O, Mn(NO 3 ) 2 ·4H 2 O, 2-methylimidazole (Aladdin, Shanghai, China); UCMSC-exo (Langfang Kangbao Huitai Biotechnology, Langfang, China); Chitosan (CS; Deacetylation degree ≥95%, Macklin, Shanghai, China), 4-carboxyphenylboronic acid; polyvinyl alcohol (4-CPBA, PVA; Aladdin, Shanghai, China); N-(3-Dimethylaminopropyl)-N′-ethyl carbodiimide hydrochloride (EDC; Aladdin, Shanghai, China); N-Hydroxysuccinimide (NHS; Aladdin, Shanghai, China); PrimeScript RT Master Mix (Takara, Beijing, China); qPCR SYBR Green Master Mix (Yeasen, Shanghai, China); CD9 antibody (Selleck, USA); TSG101 antibody (Abmart, Shanghai, China); CD86 flow antibody and CD206 flow antibody (BioLegend, USA); IL-6, CD206, HIF-1α, CK14, Ki67, CD31, VEGF and α-SMA (AiFang biological, China). ..



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    Image Search Results


    Construction and screening of phage display library . A PBMCs were isolated from camels, and RNA was extracted as a template for RT-PCR to amplify the gene sequence containing the leader signal sequence before the CH2 region. The amplification yielded two fragments: a 900-bp fragment (VH–CH1–CH2) and a 600-bp fragment (VHH–CH2). Lane M represents the DNA marker (2000–100 bp), and lanes 1–4 represent the amplified fragments. B The 600-bp fragment obtained from the first round of PCR was used as a template for the second round of PCR, which successfully generated the full-length VHH gene (spanning FR1 to FR4) with an approximate size of 400 bp. Lane M represents the DNA marker (1000–200 bp), and lanes 1–4 represent the amplified fragments. C A total of 16 clones were randomly selected from the constructed phage antibody library for identification. D Overall, 92 recombinant phage clones were randomly selected from the third round of panning and added to microplates coated with BRV. HRP-conjugated anti-M13 monoclonal antibody was used to detect the bound phages. M13K07 helper phage and PBS served as the negative control and blank control, respectively. Positive clones were defined as those with a sample-to-negative control ratio (P/N) ≥ 2.1, i.e., clones above the reference line were identified as positive.

    Journal: Veterinary Research

    Article Title: Potent neutralization and therapeutic efficacy of bovine rotavirus-specific VHH antibodies in infected calves

    doi: 10.1186/s13567-026-01765-3

    Figure Lengend Snippet: Construction and screening of phage display library . A PBMCs were isolated from camels, and RNA was extracted as a template for RT-PCR to amplify the gene sequence containing the leader signal sequence before the CH2 region. The amplification yielded two fragments: a 900-bp fragment (VH–CH1–CH2) and a 600-bp fragment (VHH–CH2). Lane M represents the DNA marker (2000–100 bp), and lanes 1–4 represent the amplified fragments. B The 600-bp fragment obtained from the first round of PCR was used as a template for the second round of PCR, which successfully generated the full-length VHH gene (spanning FR1 to FR4) with an approximate size of 400 bp. Lane M represents the DNA marker (1000–200 bp), and lanes 1–4 represent the amplified fragments. C A total of 16 clones were randomly selected from the constructed phage antibody library for identification. D Overall, 92 recombinant phage clones were randomly selected from the third round of panning and added to microplates coated with BRV. HRP-conjugated anti-M13 monoclonal antibody was used to detect the bound phages. M13K07 helper phage and PBS served as the negative control and blank control, respectively. Positive clones were defined as those with a sample-to-negative control ratio (P/N) ≥ 2.1, i.e., clones above the reference line were identified as positive.

    Article Snippet: The extracted RNA was directly subjected to one-step reverse-transcription polymerase chain reaction (RT-PCR) amplification using the PrimeScriptTM One Step RT-PCR Kit (TaKaRa, Shiga, Japan), which allows reverse transcription and PCR amplification to be conducted in a single reaction.

    Techniques: Isolation, Reverse Transcription Polymerase Chain Reaction, Sequencing, Amplification, Marker, Generated, Clone Assay, Construct, Recombinant, Negative Control, Control