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PEPperPRINT gmbh pepperchip © microarray
Panoramic Evaluation of HLA-A*02:01 Reactivity for Peptides Derived from Arbovirus. Peptide <t>microarray</t> was performed with an HLA-A*02:01 dimeric protein in three concentrations (1µg/mL, 10µg/mL and 30µg/mL). Overall fluorescence of peptide-HLA-A*02:01 reactivity (top panels), absolute number of HLA-*02:01-reactive peptides (middle panels) and mean of fluorescence intensity of HLA-*02:01-reactive peptides (lower panels) were evaluated. Chi-square test (χ2) was used to compare the number of HLA-A*02:01-reactive. Obtained p values are indicated above each bar. Comparisons regarding the mean of fluorescence intensity of HLA*02:01-reactive peptides were performed using the Kruskal-Wallis. Statistical significances were considered for p < 0.05 and are indicated by connecting lines.
Pepperchip © Microarray, supplied by PEPperPRINT gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Article Title: In silico and in vitro arboviral MHC class I-restricted-epitope signatures reveal immunodominance and poor overlapping patterns

Journal: Frontiers in Immunology

doi: 10.3389/fimmu.2022.1035515

Panoramic Evaluation of HLA-A*02:01 Reactivity for Peptides Derived from Arbovirus. Peptide microarray was performed with an HLA-A*02:01 dimeric protein in three concentrations (1µg/mL, 10µg/mL and 30µg/mL). Overall fluorescence of peptide-HLA-A*02:01 reactivity (top panels), absolute number of HLA-*02:01-reactive peptides (middle panels) and mean of fluorescence intensity of HLA-*02:01-reactive peptides (lower panels) were evaluated. Chi-square test (χ2) was used to compare the number of HLA-A*02:01-reactive. Obtained p values are indicated above each bar. Comparisons regarding the mean of fluorescence intensity of HLA*02:01-reactive peptides were performed using the Kruskal-Wallis. Statistical significances were considered for p < 0.05 and are indicated by connecting lines.
Figure Legend Snippet: Panoramic Evaluation of HLA-A*02:01 Reactivity for Peptides Derived from Arbovirus. Peptide microarray was performed with an HLA-A*02:01 dimeric protein in three concentrations (1µg/mL, 10µg/mL and 30µg/mL). Overall fluorescence of peptide-HLA-A*02:01 reactivity (top panels), absolute number of HLA-*02:01-reactive peptides (middle panels) and mean of fluorescence intensity of HLA-*02:01-reactive peptides (lower panels) were evaluated. Chi-square test (χ2) was used to compare the number of HLA-A*02:01-reactive. Obtained p values are indicated above each bar. Comparisons regarding the mean of fluorescence intensity of HLA*02:01-reactive peptides were performed using the Kruskal-Wallis. Statistical significances were considered for p < 0.05 and are indicated by connecting lines.

Techniques Used: Derivative Assay, Peptide Microarray, Fluorescence

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Article Title: First-in-human stage III/IV melanoma​ clinical trial of immune priming agent IFx-Hu2.0
Article Snippet: Diluted samples were screened on PEPperPRINT PEPperCHIP® Melanoma Antigen Microarray for 21 melanoma antigens as per manufacturer instructions [18,19].

Article Title: In silico and in vitro arboviral MHC class I-restricted-epitope signatures reveal immunodominance and poor overlapping patterns
Article Snippet: Evaluation of the HLA-A*02-restricted immunodominant peptide repertoire was performed using PEPperCHIP © (PEPperPRINT © , Heidelberg, Germany) custom peptide microarray ( ).

Article Title: In silico and in vitro arboviral MHC class I-restricted-epitope signatures reveal immunodominance and poor overlapping patterns.
Article Snippet: Supplementary Figure 1 shows a schematic compendium for the detection of HLA-A2-peptide binding using PEPperCHIP© microarray (PEPperPRINT©, Heidelberg, Germany).

Microarray:

Article Title: First-in-Human Stage III/IV Melanoma Clinical Trial of Immune Priming Agent IFx-Hu2.0
Article Snippet: .. Diluted samples were screened on PEPperPRINT PEPperCHIP Melanoma Antigen Microarray for 21 melanoma antigens as per the manufacturer’s instructions ( , ). ..

Article Title: In silico and in vitro arboviral MHC class I-restricted-epitope signatures reveal immunodominance and poor overlapping patterns.
Article Snippet: .. The PEPperCHIP© microarray slide (PEPperPRINT©, Heidelberg, Germany) was digitized on the Affymetrix 428 Array Scanner device (Thermo Fisher, California, USA). .. Data regarding HLAA2:b2M:Ig reactivity to adsorbed peptides was pre-analyzed using PepSlide® Analyzer (PEPperPRINT ©, Heidelberg, Germany) and quantified in 16 bit grey-scale images.

Article Title: Funneling modulatory peptide design with generative models: Discovery and characterization of disruptors of calcineurin protein-protein interactions
Article Snippet: .. In parallel, selected peptides were tested for Cn binding on a chip microarray (PEPperPRINT). ..

Produced:

Article Title: A quantitative and site-specific atlas of the citrullinome reveals widespread existence of citrullination and insights into PADI4 substrates
Article Snippet: .. Peptide microarrays were produced in collaboration with PEPperPRINT, according to their custom PEPperCHIP Discovery microarrays workflow. ..

Binding Assay:

Article Title: Funneling modulatory peptide design with generative models: Discovery and characterization of disruptors of calcineurin protein-protein interactions
Article Snippet: .. In parallel, selected peptides were tested for Cn binding on a chip microarray (PEPperPRINT). ..



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Panoramic Evaluation of HLA-A*02:01 Reactivity for Peptides Derived from Arbovirus. Peptide <t>microarray</t> was performed with an HLA-A*02:01 dimeric protein in three concentrations (1µg/mL, 10µg/mL and 30µg/mL). Overall fluorescence of peptide-HLA-A*02:01 reactivity (top panels), absolute number of HLA-*02:01-reactive peptides (middle panels) and mean of fluorescence intensity of HLA-*02:01-reactive peptides (lower panels) were evaluated. Chi-square test (χ2) was used to compare the number of HLA-A*02:01-reactive. Obtained p values are indicated above each bar. Comparisons regarding the mean of fluorescence intensity of HLA*02:01-reactive peptides were performed using the Kruskal-Wallis. Statistical significances were considered for p < 0.05 and are indicated by connecting lines.
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Panoramic Evaluation of HLA-A*02:01 Reactivity for Peptides Derived from Arbovirus. Peptide microarray was performed with an HLA-A*02:01 dimeric protein in three concentrations (1µg/mL, 10µg/mL and 30µg/mL). Overall fluorescence of peptide-HLA-A*02:01 reactivity (top panels), absolute number of HLA-*02:01-reactive peptides (middle panels) and mean of fluorescence intensity of HLA-*02:01-reactive peptides (lower panels) were evaluated. Chi-square test (χ2) was used to compare the number of HLA-A*02:01-reactive. Obtained p values are indicated above each bar. Comparisons regarding the mean of fluorescence intensity of HLA*02:01-reactive peptides were performed using the Kruskal-Wallis. Statistical significances were considered for p < 0.05 and are indicated by connecting lines.

Journal: Frontiers in Immunology

Article Title: In silico and in vitro arboviral MHC class I-restricted-epitope signatures reveal immunodominance and poor overlapping patterns

doi: 10.3389/fimmu.2022.1035515

Figure Lengend Snippet: Panoramic Evaluation of HLA-A*02:01 Reactivity for Peptides Derived from Arbovirus. Peptide microarray was performed with an HLA-A*02:01 dimeric protein in three concentrations (1µg/mL, 10µg/mL and 30µg/mL). Overall fluorescence of peptide-HLA-A*02:01 reactivity (top panels), absolute number of HLA-*02:01-reactive peptides (middle panels) and mean of fluorescence intensity of HLA-*02:01-reactive peptides (lower panels) were evaluated. Chi-square test (χ2) was used to compare the number of HLA-A*02:01-reactive. Obtained p values are indicated above each bar. Comparisons regarding the mean of fluorescence intensity of HLA*02:01-reactive peptides were performed using the Kruskal-Wallis. Statistical significances were considered for p < 0.05 and are indicated by connecting lines.

Article Snippet: Evaluation of the HLA-A*02-restricted immunodominant peptide repertoire was performed using PEPperCHIP © (PEPperPRINT © , Heidelberg, Germany) custom peptide microarray ( ).

Techniques: Derivative Assay, Peptide Microarray, Fluorescence