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Incyte corporation cdna microarray experiment
Cdna Microarray Experiment, supplied by Incyte corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/oligonucleotide+microarray+experiments/cdna+microarray/pm15520318-40-5-12
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cdna microarray experiment - by Bioz Stars, 2026-09
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Related Articles

Microarray:

Article Title: High-Throughput Transcriptome Profiling in Drug and Biomarker Discovery
Article Snippet: .. They used cDNA microarray tools (Incyte, Inc., MO, U.S.A.) to identify the candidate genes responsible for enhancing memory and evaluate the specific gene expression patterns by real-time PCR in SD rat model of passive avoidance task. ..

Article Title: ASBMR 23nd annual meeting.
Article Snippet: .. In this study, cDNA microarray analysis was conducted by Incyte Genomic Systems on 8,734 gene accessions using mRNA from mouse femurs (free of marrow) of both the congenic and B6 strains to test two hypotheses: 1) microarray analysis will identify candidate genes for bone density; and 2) by comparing the gene expression profile outside of the QTL region between B6 and the congenic mice, we can better characterize the molecular phenotypic mechanisms that contribute to the high bone density QTL. ..

Article Title: Identification of Novel Peroxisome Proliferator-Activated Receptor α (PPARα) Target Genes in Mouse Liver Using cDNA Microarray Analysis
Article Snippet: Poly(A) + selected RNA was isolated using the Oligotex kit (Qiagen, Valencia, CA) from wild-type untreated mice, and wild-type mice treated with Wy-14,643 (0.125%, w/w) for 2 weeks. .. Poly(A) + RNA was labeled with Cy3 and Cy5 fluorescent dyes for microarray hybridization on UniGene mouse cDNA microarray (Incyte Genomics, St Louis, MO), as described elsewhere ( 77 ). cDNA mouse clones, corresponding to spotted cDNAs on the microarray, were obtained from Incyte Genomics (St. Louis, MO), amplified and sequenced to verify the correct identity of each clone. .. The data were analyzed using Incyte GEMTools vision 2.4.2a software (Incyte).

Article Title: Differentially expressed genes in large granular lymphocyte leukemia
Article Snippet: .. Overexpression of a variety cytotoxic genes was observed in leukemic LGL utilizing cDNA microarray from Incyte Genomics (FIG. 1 and Table 4). .. To verify the identity of these overexpressed genes, clones containing cDNA fragments of the selected genes were obtained from Incyte Genomics and confirmed by sequencing.

Article Title: A review of Omics research in acupuncture: the relevance and future prospects for understanding the nature of meridians and acupoints.
Article Snippet: .. Insulin-like growth factor-1 (IGF-1) was one of the most notable up-regulated J. Jia et al. / Journal of Ethnopharm acology 140 (2012) 594– 603 597 598 J. Jia et al. / Journal of Ethnopharm acology 140 (2012) 594– 603 Table 1 (Continued) Ref/Year Acupoints Treatment Subject groups Targeted disease/symptom Tissue sample Technique Verification/ validation Identified candidate gene Implication Ko/2002 ST36 (Zusanli) EA SD rats (n = 7/group): normal/neuropathic pain/EA treatment/ Neuropathic pain RNA from spinal nerves [pooled RNA samples] cDNA microarray (Incyte genomics) Dot-blot Up: opioid receptor, MAP kinase, zinc finger protein, and tyrosine phosphatase related genes Pain and analgesic Pan/2011 MS6 (scalp acu motor areas)/BL10(Tianzhu)/GB20 (Fengchi)/ LI4 (Hegu)/PC6 (Neiguan)/BL40 (Weizhong)/SP6 (Sanyinjiao)/S36 (Zusanli) EA 35 human: drug treatment/EA + drug treatment/normal Acute ischemic stroke Serum protein 2-DE + MS/MS Western blot Up: Gelsolin, C3, down: Serpin G1 Neuroprotection Kim/2010 HT8 (Sobu) Acupuncture SD rats (n = 34): pup+ mother/pup + mother +acupuncture/maternally separated(MS)/MS + acupuncture/ Maternal separation Protein sample from hypothalamic area 2-DE + MALDITOF/TOF MS N.A. ..

Article Title: Ectonucleoside triphosphate diphosphohydrolase 6 expression in testis and testicular cancer and its implication in cisplatin resistance.
Article Snippet: After 48 h, the surviving cells were stained with the alamarBlue assay (treK Diagnostic systems, cleveland, oH, UsA) for 180 min at 37°c. the absorbances of the wells were measured using a plate reader (ArVoTM MX; perkin elmer Inc., Waltham, MA, UsA). cDNA microarray analysis. the procedure of cDnA microarray analysis was performed as described previously (18). .. Briefly, cDNA generation, hybridization, and data collection for cDnA microarray analyses were performed by the Incyte corporation (palo Alto, cA, UsA). ..

Gene Expression:

Article Title: High-Throughput Transcriptome Profiling in Drug and Biomarker Discovery
Article Snippet: .. They used cDNA microarray tools (Incyte, Inc., MO, U.S.A.) to identify the candidate genes responsible for enhancing memory and evaluate the specific gene expression patterns by real-time PCR in SD rat model of passive avoidance task. ..

Article Title: ASBMR 23nd annual meeting.
Article Snippet: .. In this study, cDNA microarray analysis was conducted by Incyte Genomic Systems on 8,734 gene accessions using mRNA from mouse femurs (free of marrow) of both the congenic and B6 strains to test two hypotheses: 1) microarray analysis will identify candidate genes for bone density; and 2) by comparing the gene expression profile outside of the QTL region between B6 and the congenic mice, we can better characterize the molecular phenotypic mechanisms that contribute to the high bone density QTL. ..

Real-time Polymerase Chain Reaction:

Article Title: High-Throughput Transcriptome Profiling in Drug and Biomarker Discovery
Article Snippet: .. They used cDNA microarray tools (Incyte, Inc., MO, U.S.A.) to identify the candidate genes responsible for enhancing memory and evaluate the specific gene expression patterns by real-time PCR in SD rat model of passive avoidance task. ..

Labeling:

Article Title: Identification of Novel Peroxisome Proliferator-Activated Receptor α (PPARα) Target Genes in Mouse Liver Using cDNA Microarray Analysis
Article Snippet: Poly(A) + selected RNA was isolated using the Oligotex kit (Qiagen, Valencia, CA) from wild-type untreated mice, and wild-type mice treated with Wy-14,643 (0.125%, w/w) for 2 weeks. .. Poly(A) + RNA was labeled with Cy3 and Cy5 fluorescent dyes for microarray hybridization on UniGene mouse cDNA microarray (Incyte Genomics, St Louis, MO), as described elsewhere ( 77 ). cDNA mouse clones, corresponding to spotted cDNAs on the microarray, were obtained from Incyte Genomics (St. Louis, MO), amplified and sequenced to verify the correct identity of each clone. .. The data were analyzed using Incyte GEMTools vision 2.4.2a software (Incyte).

Hybridization:

Article Title: Identification of Novel Peroxisome Proliferator-Activated Receptor α (PPARα) Target Genes in Mouse Liver Using cDNA Microarray Analysis
Article Snippet: Poly(A) + selected RNA was isolated using the Oligotex kit (Qiagen, Valencia, CA) from wild-type untreated mice, and wild-type mice treated with Wy-14,643 (0.125%, w/w) for 2 weeks. .. Poly(A) + RNA was labeled with Cy3 and Cy5 fluorescent dyes for microarray hybridization on UniGene mouse cDNA microarray (Incyte Genomics, St Louis, MO), as described elsewhere ( 77 ). cDNA mouse clones, corresponding to spotted cDNAs on the microarray, were obtained from Incyte Genomics (St. Louis, MO), amplified and sequenced to verify the correct identity of each clone. .. The data were analyzed using Incyte GEMTools vision 2.4.2a software (Incyte).

Article Title: Ectonucleoside triphosphate diphosphohydrolase 6 expression in testis and testicular cancer and its implication in cisplatin resistance.
Article Snippet: After 48 h, the surviving cells were stained with the alamarBlue assay (treK Diagnostic systems, cleveland, oH, UsA) for 180 min at 37°c. the absorbances of the wells were measured using a plate reader (ArVoTM MX; perkin elmer Inc., Waltham, MA, UsA). cDNA microarray analysis. the procedure of cDnA microarray analysis was performed as described previously (18). .. Briefly, cDNA generation, hybridization, and data collection for cDnA microarray analyses were performed by the Incyte corporation (palo Alto, cA, UsA). ..

Clone Assay:

Article Title: Identification of Novel Peroxisome Proliferator-Activated Receptor α (PPARα) Target Genes in Mouse Liver Using cDNA Microarray Analysis
Article Snippet: Poly(A) + selected RNA was isolated using the Oligotex kit (Qiagen, Valencia, CA) from wild-type untreated mice, and wild-type mice treated with Wy-14,643 (0.125%, w/w) for 2 weeks. .. Poly(A) + RNA was labeled with Cy3 and Cy5 fluorescent dyes for microarray hybridization on UniGene mouse cDNA microarray (Incyte Genomics, St Louis, MO), as described elsewhere ( 77 ). cDNA mouse clones, corresponding to spotted cDNAs on the microarray, were obtained from Incyte Genomics (St. Louis, MO), amplified and sequenced to verify the correct identity of each clone. .. The data were analyzed using Incyte GEMTools vision 2.4.2a software (Incyte).

Amplification:

Article Title: Identification of Novel Peroxisome Proliferator-Activated Receptor α (PPARα) Target Genes in Mouse Liver Using cDNA Microarray Analysis
Article Snippet: Poly(A) + selected RNA was isolated using the Oligotex kit (Qiagen, Valencia, CA) from wild-type untreated mice, and wild-type mice treated with Wy-14,643 (0.125%, w/w) for 2 weeks. .. Poly(A) + RNA was labeled with Cy3 and Cy5 fluorescent dyes for microarray hybridization on UniGene mouse cDNA microarray (Incyte Genomics, St Louis, MO), as described elsewhere ( 77 ). cDNA mouse clones, corresponding to spotted cDNAs on the microarray, were obtained from Incyte Genomics (St. Louis, MO), amplified and sequenced to verify the correct identity of each clone. .. The data were analyzed using Incyte GEMTools vision 2.4.2a software (Incyte).

Over Expression:

Article Title: Differentially expressed genes in large granular lymphocyte leukemia
Article Snippet: .. Overexpression of a variety cytotoxic genes was observed in leukemic LGL utilizing cDNA microarray from Incyte Genomics (FIG. 1 and Table 4). .. To verify the identity of these overexpressed genes, clones containing cDNA fragments of the selected genes were obtained from Incyte Genomics and confirmed by sequencing.

other:

Article Title: Three microarray platforms: an analysis of their concordance in profiling gene expression
Article Snippet: Incyte cDNA arrays and Operon long oligonucleotide (70-mer) arrays were printed in house, and Affymetrix 25-mer HG U133A arrays were purchased from the manufacturer.

Article Title: Multifactorial regulation of E-cadherin expression: an integrative study
Article Snippet: We have previously described our processing and normalization of NCI-60 transcript expression data from pin-spotted cDNA arrays (Incyte, Inc., Palo Alto, CA) ( 27 , 28 ), Affymetrix Hu-6800 arrays (Affymetrix, Sunnyvale, CA) ( 29 ), Affymetrix HG-U95 arrays ( 30 ), and Affymetrix HG-U133 ( 30 ).

Biomarker Discovery:

Article Title: A review of Omics research in acupuncture: the relevance and future prospects for understanding the nature of meridians and acupoints.
Article Snippet: .. Insulin-like growth factor-1 (IGF-1) was one of the most notable up-regulated J. Jia et al. / Journal of Ethnopharm acology 140 (2012) 594– 603 597 598 J. Jia et al. / Journal of Ethnopharm acology 140 (2012) 594– 603 Table 1 (Continued) Ref/Year Acupoints Treatment Subject groups Targeted disease/symptom Tissue sample Technique Verification/ validation Identified candidate gene Implication Ko/2002 ST36 (Zusanli) EA SD rats (n = 7/group): normal/neuropathic pain/EA treatment/ Neuropathic pain RNA from spinal nerves [pooled RNA samples] cDNA microarray (Incyte genomics) Dot-blot Up: opioid receptor, MAP kinase, zinc finger protein, and tyrosine phosphatase related genes Pain and analgesic Pan/2011 MS6 (scalp acu motor areas)/BL10(Tianzhu)/GB20 (Fengchi)/ LI4 (Hegu)/PC6 (Neiguan)/BL40 (Weizhong)/SP6 (Sanyinjiao)/S36 (Zusanli) EA 35 human: drug treatment/EA + drug treatment/normal Acute ischemic stroke Serum protein 2-DE + MS/MS Western blot Up: Gelsolin, C3, down: Serpin G1 Neuroprotection Kim/2010 HT8 (Sobu) Acupuncture SD rats (n = 34): pup+ mother/pup + mother +acupuncture/maternally separated(MS)/MS + acupuncture/ Maternal separation Protein sample from hypothalamic area 2-DE + MALDITOF/TOF MS N.A. ..

Tandem Mass Spectroscopy:

Article Title: A review of Omics research in acupuncture: the relevance and future prospects for understanding the nature of meridians and acupoints.
Article Snippet: .. Insulin-like growth factor-1 (IGF-1) was one of the most notable up-regulated J. Jia et al. / Journal of Ethnopharm acology 140 (2012) 594– 603 597 598 J. Jia et al. / Journal of Ethnopharm acology 140 (2012) 594– 603 Table 1 (Continued) Ref/Year Acupoints Treatment Subject groups Targeted disease/symptom Tissue sample Technique Verification/ validation Identified candidate gene Implication Ko/2002 ST36 (Zusanli) EA SD rats (n = 7/group): normal/neuropathic pain/EA treatment/ Neuropathic pain RNA from spinal nerves [pooled RNA samples] cDNA microarray (Incyte genomics) Dot-blot Up: opioid receptor, MAP kinase, zinc finger protein, and tyrosine phosphatase related genes Pain and analgesic Pan/2011 MS6 (scalp acu motor areas)/BL10(Tianzhu)/GB20 (Fengchi)/ LI4 (Hegu)/PC6 (Neiguan)/BL40 (Weizhong)/SP6 (Sanyinjiao)/S36 (Zusanli) EA 35 human: drug treatment/EA + drug treatment/normal Acute ischemic stroke Serum protein 2-DE + MS/MS Western blot Up: Gelsolin, C3, down: Serpin G1 Neuroprotection Kim/2010 HT8 (Sobu) Acupuncture SD rats (n = 34): pup+ mother/pup + mother +acupuncture/maternally separated(MS)/MS + acupuncture/ Maternal separation Protein sample from hypothalamic area 2-DE + MALDITOF/TOF MS N.A. ..

Western Blot:

Article Title: A review of Omics research in acupuncture: the relevance and future prospects for understanding the nature of meridians and acupoints.
Article Snippet: .. Insulin-like growth factor-1 (IGF-1) was one of the most notable up-regulated J. Jia et al. / Journal of Ethnopharm acology 140 (2012) 594– 603 597 598 J. Jia et al. / Journal of Ethnopharm acology 140 (2012) 594– 603 Table 1 (Continued) Ref/Year Acupoints Treatment Subject groups Targeted disease/symptom Tissue sample Technique Verification/ validation Identified candidate gene Implication Ko/2002 ST36 (Zusanli) EA SD rats (n = 7/group): normal/neuropathic pain/EA treatment/ Neuropathic pain RNA from spinal nerves [pooled RNA samples] cDNA microarray (Incyte genomics) Dot-blot Up: opioid receptor, MAP kinase, zinc finger protein, and tyrosine phosphatase related genes Pain and analgesic Pan/2011 MS6 (scalp acu motor areas)/BL10(Tianzhu)/GB20 (Fengchi)/ LI4 (Hegu)/PC6 (Neiguan)/BL40 (Weizhong)/SP6 (Sanyinjiao)/S36 (Zusanli) EA 35 human: drug treatment/EA + drug treatment/normal Acute ischemic stroke Serum protein 2-DE + MS/MS Western blot Up: Gelsolin, C3, down: Serpin G1 Neuroprotection Kim/2010 HT8 (Sobu) Acupuncture SD rats (n = 34): pup+ mother/pup + mother +acupuncture/maternally separated(MS)/MS + acupuncture/ Maternal separation Protein sample from hypothalamic area 2-DE + MALDITOF/TOF MS N.A. ..



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A) The stacked bar chart represents a summary of total upregulated (red) and downregulated (green) genes representing 22 important signaling and disease pathways in AD subjects compared to controls. The output core analysis, reflecting the differential gene expressions obtained from the microarrays (gene sets with≥2-fold change, t -test, p < 0.05). B) Ingenuity Pathway Analysis (IPA)-derived Amyloid Processing network of differentially expressed genes derived from microarray analysis. IPA analysis identified a group of genes expression status and their potential interactive links in the context of Amyloid Processing, Neuronal Death. We noted activation of Gamma Secretase, Beta Secretase, upregulation of ERK1/2 CK1/2 P38MAPK, PKA, PRKCE, CDK5 , and CDK5R1 and downregulation of MAPT , and GSK3B .

Journal: Journal of Alzheimer's Disease Reports

Article Title: Transcriptomic Analysis of Alzheimer’s Disease Pathways in a Pakistani Population 1

doi: 10.3233/ADR-230146

Figure Lengend Snippet: A) The stacked bar chart represents a summary of total upregulated (red) and downregulated (green) genes representing 22 important signaling and disease pathways in AD subjects compared to controls. The output core analysis, reflecting the differential gene expressions obtained from the microarrays (gene sets with≥2-fold change, t -test, p < 0.05). B) Ingenuity Pathway Analysis (IPA)-derived Amyloid Processing network of differentially expressed genes derived from microarray analysis. IPA analysis identified a group of genes expression status and their potential interactive links in the context of Amyloid Processing, Neuronal Death. We noted activation of Gamma Secretase, Beta Secretase, upregulation of ERK1/2 CK1/2 P38MAPK, PKA, PRKCE, CDK5 , and CDK5R1 and downregulation of MAPT , and GSK3B .

Article Snippet: The oligonucleotide microarray experiments were conducted by EpigenDx (Boston, MA) using the Affymetrix U133 Plus 2.0 Array platform, which has comprehensive coverage of the whole transcribed human genome on a single array.

Techniques: Derivative Assay, Microarray, Expressing, Activation Assay

Comparison of transcript quantification by Affymetrix microarray with real time RT-PCR. A 200 ng aliquot total RNA from dDAVP- and vehicle-exposed mpkCCD cells was used for quantification of transcript abundances with real time RT-PCR. *, statistically significant versus no change i.e. log2(dDAVP/vehicle) = 0 (p < 0.05, n = 3). The RefSeq accession numbers are: Akap12, NM_031185; Aqp2, NM_009699; Asap2, NM_001004364; C3, NM_009778; Clmn, NM_001040682; Cpt1a, NM_013495; Fth1, NM_010239; Gsdmc1, NM_031378; Gsdmc2, NM_177912; Gsdmc4, XM_001474104; Gstt3, NM_133994; Idh1, NM_001111320; Mon2, NM_153395; Osbpl1a, NM_207530; Spnb3, NM_021287; and Trip11, XM_001001171.

Journal: Molecular & Cellular Proteomics : MCP

Article Title: Quantitative Protein and mRNA Profiling Shows Selective Post-Transcriptional Control of Protein Expression by Vasopressin in Kidney Cells *

doi: 10.1074/mcp.M110.004036

Figure Lengend Snippet: Comparison of transcript quantification by Affymetrix microarray with real time RT-PCR. A 200 ng aliquot total RNA from dDAVP- and vehicle-exposed mpkCCD cells was used for quantification of transcript abundances with real time RT-PCR. *, statistically significant versus no change i.e. log2(dDAVP/vehicle) = 0 (p < 0.05, n = 3). The RefSeq accession numbers are: Akap12, NM_031185; Aqp2, NM_009699; Asap2, NM_001004364; C3, NM_009778; Clmn, NM_001040682; Cpt1a, NM_013495; Fth1, NM_010239; Gsdmc1, NM_031378; Gsdmc2, NM_177912; Gsdmc4, XM_001474104; Gstt3, NM_133994; Idh1, NM_001111320; Mon2, NM_153395; Osbpl1a, NM_207530; Spnb3, NM_021287; and Trip11, XM_001001171.

Article Snippet: To address whether changes in protein abundance measured by SILAC LC-MS/MS are generally because of corresponding changes in mRNA levels, we carried out oligonucleotide microarray experiments (Affymetrix) in mpkCCD cells treated in the same manner (0.1 n m dDAVP or vehicle for 5 days).

Techniques: Microarray, Quantitative RT-PCR