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genechip expression analysis—oligonucleotide microarray experiments  (Thermo Fisher)


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    Thermo Fisher genechip expression analysis—oligonucleotide microarray experiments
    Genechip Expression Analysis—Oligonucleotide Microarray Experiments, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/oligonucleotide+microarray+experiments/10__1074_slash_jbc__m302128200-56-1-0
    Average 90 stars, based on 1 article reviews
    genechip expression analysis—oligonucleotide microarray experiments - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Amplification:

    Article Title: Relationship of paroxetine disposition to metoprolol metabolic ratio and CYP2D6*10 genotype of Korean subjects.
    Article Snippet: Objective: To evaluate the relationship between the metabolic ratio (MR) of metoprolol, CYP2D6*10B genotype, and the disposition of paroxetine in Korean subjects.. Methods: A single 40-mg dose of paroxetine was administered orally to one poor metabolizer and 15 healthy subjects recruited from 223 Korean extensive metabolizers whose phenotypes were predetermined by use of the metoprolol MR. Genotypes were determined by allele-specific polymerase chain reaction and the GeneChip microarray technique.. Pharmacokinetic parameters were estimated from plasma concentrations of paroxetine for more than 240 hours after the oral dose.

    Microarray:

    Article Title: Relationship of paroxetine disposition to metoprolol metabolic ratio and CYP2D6*10 genotype of Korean subjects.
    Article Snippet: Objective: To evaluate the relationship between the metabolic ratio (MR) of metoprolol, CYP2D6*10B genotype, and the disposition of paroxetine in Korean subjects.. Methods: A single 40-mg dose of paroxetine was administered orally to one poor metabolizer and 15 healthy subjects recruited from 223 Korean extensive metabolizers whose phenotypes were predetermined by use of the metoprolol MR. Genotypes were determined by allele-specific polymerase chain reaction and the GeneChip microarray technique.. Pharmacokinetic parameters were estimated from plasma concentrations of paroxetine for more than 240 hours after the oral dose.

    Article Title: Method for amplification of nucleotide sequence
    Article Snippet: The group of labeled nucleic acids may be applied to a series of oligonucleotide microarray GeneChip (manufactured by Affymetrix, Inc.) that is able to simultaneously measure the expression levels of plural genes, and is currently being a main stream. .. The group of labeled nucleic acids may be applied to a series of oligonucleotide microarray GeneChip (manufactured by Affymetrix, Inc.) that is able to simultaneously measure the expression levels of plural genes, and is currently being a main stream. .. In another aspect, the present invention provides an method for amplification of the nucleotide sequence including steps (a) to (e) in the method for preparing the group of nucleic acids of the present invention.

    Article Title: Correlation network analysis for data integration and biomarker selection.
    Article Snippet: RNA was isolated from frozen liver tissue using an internal protocol (AstraZeneca, Södertälje, Sweden). .. Affymetrix GeneChip oligonucleotide microarray analysis was carried out on labeled and fragmented cRNA prepared from total RNA with the Affymetrix U34A array, version December 2003, and analyzed using the Affymetrix GeneChip Scanner system (Affymetrix, Santa Clara, CA). .. The CEL files were quantile normalized, and positional-dependent nearest-neighbor output was computed for subsequent analysis as reported elsewhere.31 The resulting quantification from the Affymetrix GeneChip platform is relative and not absolute.

    Article Title: Abstracts
    Article Snippet: .. In order to identify genes associated with meningioma tumorigenesis in general and development of MM in particular, we have used a global survey of gene expression by Affymetrix oligonucleotide microarray GeneChip® technology. ..

    Article Title: Lymphoid Tissue and Emphysema in the Lungs of Transgenic Mice Inducibly Expressing Tumor Necrosis Factor-α
    Article Snippet: To develop a model in which the pathogenic effects of the proinflammatory cytokine tumor necrosis factor(TNF) could be investigated, transgenic mice that express TNF in the lung under the control of a doxycycline-inducible promoter were generated.. TNF transgene message was expressed at a low level in the absence of doxycycline treatment and was induced in the lung by administration of the drug.. Analysis of lung lavage fluid indicated increases in neutrophils and lymphocytes in doxycycline-treated transgenic mice.

    Mutagenesis:

    Article Title: Relationship of paroxetine disposition to metoprolol metabolic ratio and CYP2D6*10 genotype of Korean subjects.
    Article Snippet: Objective: To evaluate the relationship between the metabolic ratio (MR) of metoprolol, CYP2D6*10B genotype, and the disposition of paroxetine in Korean subjects.. Methods: A single 40-mg dose of paroxetine was administered orally to one poor metabolizer and 15 healthy subjects recruited from 223 Korean extensive metabolizers whose phenotypes were predetermined by use of the metoprolol MR. Genotypes were determined by allele-specific polymerase chain reaction and the GeneChip microarray technique.. Pharmacokinetic parameters were estimated from plasma concentrations of paroxetine for more than 240 hours after the oral dose.

    Activity Assay:

    Article Title: Relationship of paroxetine disposition to metoprolol metabolic ratio and CYP2D6*10 genotype of Korean subjects.
    Article Snippet: Objective: To evaluate the relationship between the metabolic ratio (MR) of metoprolol, CYP2D6*10B genotype, and the disposition of paroxetine in Korean subjects.. Methods: A single 40-mg dose of paroxetine was administered orally to one poor metabolizer and 15 healthy subjects recruited from 223 Korean extensive metabolizers whose phenotypes were predetermined by use of the metoprolol MR. Genotypes were determined by allele-specific polymerase chain reaction and the GeneChip microarray technique.. Pharmacokinetic parameters were estimated from plasma concentrations of paroxetine for more than 240 hours after the oral dose.

    Labeling:

    Article Title: Method for amplification of nucleotide sequence
    Article Snippet: The group of labeled nucleic acids may be applied to a series of oligonucleotide microarray GeneChip (manufactured by Affymetrix, Inc.) that is able to simultaneously measure the expression levels of plural genes, and is currently being a main stream. .. The group of labeled nucleic acids may be applied to a series of oligonucleotide microarray GeneChip (manufactured by Affymetrix, Inc.) that is able to simultaneously measure the expression levels of plural genes, and is currently being a main stream. .. In another aspect, the present invention provides an method for amplification of the nucleotide sequence including steps (a) to (e) in the method for preparing the group of nucleic acids of the present invention.

    Article Title: Correlation network analysis for data integration and biomarker selection.
    Article Snippet: RNA was isolated from frozen liver tissue using an internal protocol (AstraZeneca, Södertälje, Sweden). .. Affymetrix GeneChip oligonucleotide microarray analysis was carried out on labeled and fragmented cRNA prepared from total RNA with the Affymetrix U34A array, version December 2003, and analyzed using the Affymetrix GeneChip Scanner system (Affymetrix, Santa Clara, CA). .. The CEL files were quantile normalized, and positional-dependent nearest-neighbor output was computed for subsequent analysis as reported elsewhere.31 The resulting quantification from the Affymetrix GeneChip platform is relative and not absolute.

    Expressing:

    Article Title: Method for amplification of nucleotide sequence
    Article Snippet: The group of labeled nucleic acids may be applied to a series of oligonucleotide microarray GeneChip (manufactured by Affymetrix, Inc.) that is able to simultaneously measure the expression levels of plural genes, and is currently being a main stream. .. The group of labeled nucleic acids may be applied to a series of oligonucleotide microarray GeneChip (manufactured by Affymetrix, Inc.) that is able to simultaneously measure the expression levels of plural genes, and is currently being a main stream. .. In another aspect, the present invention provides an method for amplification of the nucleotide sequence including steps (a) to (e) in the method for preparing the group of nucleic acids of the present invention.

    Article Title: Lymphoid Tissue and Emphysema in the Lungs of Transgenic Mice Inducibly Expressing Tumor Necrosis Factor-α
    Article Snippet: To develop a model in which the pathogenic effects of the proinflammatory cytokine tumor necrosis factor(TNF) could be investigated, transgenic mice that express TNF in the lung under the control of a doxycycline-inducible promoter were generated.. TNF transgene message was expressed at a low level in the absence of doxycycline treatment and was induced in the lung by administration of the drug.. Analysis of lung lavage fluid indicated increases in neutrophils and lymphocytes in doxycycline-treated transgenic mice.

    Gene Expression:

    Article Title: Abstracts
    Article Snippet: .. In order to identify genes associated with meningioma tumorigenesis in general and development of MM in particular, we have used a global survey of gene expression by Affymetrix oligonucleotide microarray GeneChip® technology. ..

    other:

    Article Title: Farnesoid X Receptor Regulates Bile Acid-Amino Acid Conjugation
    Article Snippet: Affymetrix GeneChip Expression Analysis—Oligonucleotide microarray experiments were performed on 10 g of total RNA and analyzed according to protocols developed by Affymetrix (Santa Clarita, CA).



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    A) The stacked bar chart represents a summary of total upregulated (red) and downregulated (green) genes representing 22 important signaling and disease pathways in AD subjects compared to controls. The output core analysis, reflecting the differential gene expressions obtained from the microarrays (gene sets with≥2-fold change, t -test, p < 0.05). B) Ingenuity Pathway Analysis (IPA)-derived Amyloid Processing network of differentially expressed genes derived from <t>microarray</t> analysis. IPA analysis identified a group of genes expression status and their potential interactive links in the context of Amyloid Processing, Neuronal Death. We noted activation of Gamma Secretase, Beta Secretase, upregulation of ERK1/2 CK1/2 P38MAPK, PKA, PRKCE, CDK5 , and CDK5R1 and downregulation of MAPT , and GSK3B .
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    Comparison of transcript quantification by Affymetrix <t>microarray</t> with real time RT-PCR. A 200 ng aliquot total RNA from dDAVP- and vehicle-exposed mpkCCD cells was used for quantification of transcript abundances with real time RT-PCR. *, statistically significant versus no change i.e. log2(dDAVP/vehicle) = 0 (p < 0.05, n = 3). The RefSeq accession numbers are: Akap12, NM_031185; Aqp2, NM_009699; Asap2, NM_001004364; C3, NM_009778; Clmn, NM_001040682; Cpt1a, NM_013495; Fth1, NM_010239; Gsdmc1, NM_031378; Gsdmc2, NM_177912; Gsdmc4, XM_001474104; Gstt3, NM_133994; Idh1, NM_001111320; Mon2, NM_153395; Osbpl1a, NM_207530; Spnb3, NM_021287; and Trip11, XM_001001171.
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    Image Search Results


    A) The stacked bar chart represents a summary of total upregulated (red) and downregulated (green) genes representing 22 important signaling and disease pathways in AD subjects compared to controls. The output core analysis, reflecting the differential gene expressions obtained from the microarrays (gene sets with≥2-fold change, t -test, p < 0.05). B) Ingenuity Pathway Analysis (IPA)-derived Amyloid Processing network of differentially expressed genes derived from microarray analysis. IPA analysis identified a group of genes expression status and their potential interactive links in the context of Amyloid Processing, Neuronal Death. We noted activation of Gamma Secretase, Beta Secretase, upregulation of ERK1/2 CK1/2 P38MAPK, PKA, PRKCE, CDK5 , and CDK5R1 and downregulation of MAPT , and GSK3B .

    Journal: Journal of Alzheimer's Disease Reports

    Article Title: Transcriptomic Analysis of Alzheimer’s Disease Pathways in a Pakistani Population 1

    doi: 10.3233/ADR-230146

    Figure Lengend Snippet: A) The stacked bar chart represents a summary of total upregulated (red) and downregulated (green) genes representing 22 important signaling and disease pathways in AD subjects compared to controls. The output core analysis, reflecting the differential gene expressions obtained from the microarrays (gene sets with≥2-fold change, t -test, p < 0.05). B) Ingenuity Pathway Analysis (IPA)-derived Amyloid Processing network of differentially expressed genes derived from microarray analysis. IPA analysis identified a group of genes expression status and their potential interactive links in the context of Amyloid Processing, Neuronal Death. We noted activation of Gamma Secretase, Beta Secretase, upregulation of ERK1/2 CK1/2 P38MAPK, PKA, PRKCE, CDK5 , and CDK5R1 and downregulation of MAPT , and GSK3B .

    Article Snippet: The oligonucleotide microarray experiments were conducted by EpigenDx (Boston, MA) using the Affymetrix U133 Plus 2.0 Array platform, which has comprehensive coverage of the whole transcribed human genome on a single array.

    Techniques: Derivative Assay, Microarray, Expressing, Activation Assay

    Comparison of transcript quantification by Affymetrix microarray with real time RT-PCR. A 200 ng aliquot total RNA from dDAVP- and vehicle-exposed mpkCCD cells was used for quantification of transcript abundances with real time RT-PCR. *, statistically significant versus no change i.e. log2(dDAVP/vehicle) = 0 (p < 0.05, n = 3). The RefSeq accession numbers are: Akap12, NM_031185; Aqp2, NM_009699; Asap2, NM_001004364; C3, NM_009778; Clmn, NM_001040682; Cpt1a, NM_013495; Fth1, NM_010239; Gsdmc1, NM_031378; Gsdmc2, NM_177912; Gsdmc4, XM_001474104; Gstt3, NM_133994; Idh1, NM_001111320; Mon2, NM_153395; Osbpl1a, NM_207530; Spnb3, NM_021287; and Trip11, XM_001001171.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: Quantitative Protein and mRNA Profiling Shows Selective Post-Transcriptional Control of Protein Expression by Vasopressin in Kidney Cells *

    doi: 10.1074/mcp.M110.004036

    Figure Lengend Snippet: Comparison of transcript quantification by Affymetrix microarray with real time RT-PCR. A 200 ng aliquot total RNA from dDAVP- and vehicle-exposed mpkCCD cells was used for quantification of transcript abundances with real time RT-PCR. *, statistically significant versus no change i.e. log2(dDAVP/vehicle) = 0 (p < 0.05, n = 3). The RefSeq accession numbers are: Akap12, NM_031185; Aqp2, NM_009699; Asap2, NM_001004364; C3, NM_009778; Clmn, NM_001040682; Cpt1a, NM_013495; Fth1, NM_010239; Gsdmc1, NM_031378; Gsdmc2, NM_177912; Gsdmc4, XM_001474104; Gstt3, NM_133994; Idh1, NM_001111320; Mon2, NM_153395; Osbpl1a, NM_207530; Spnb3, NM_021287; and Trip11, XM_001001171.

    Article Snippet: To address whether changes in protein abundance measured by SILAC LC-MS/MS are generally because of corresponding changes in mRNA levels, we carried out oligonucleotide microarray experiments (Affymetrix) in mpkCCD cells treated in the same manner (0.1 n m dDAVP or vehicle for 5 days).

    Techniques: Microarray, Quantitative RT-PCR