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nih3t3 l1  (ATCC)


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    Structured Review

    ATCC nih3t3 l1
    Nih3t3 L1, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 7072 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nih3t3/3T3-L1/pm42225060-782-22-23
    Average 99 stars, based on 7072 article reviews
    nih3t3 l1 - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Insulin-stimulated phosphorylation of CLASP2 by ERK regulates microtubule plus-end binding of CLASP2 and insulin-regulated microtubule stabilization.
    Article Snippet: .. Mouse 3T3-L1 fibroblasts (ATCC, cat no CL-173) were cultured and differentiated into adipocytes exactly as previously described (Kruse et al., 2017; Parker et al., 2019). .. Chinese hamster ovary (CHO) cells expressing the human IR (CHO/IR) cells have been described previously (Liu and Roth, 1995; Dong et al., 1997) and were grown in HAM'S F-12 medium (ThermoFisher Scientific, cat. # 31765035, Waltham, MA), supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin.

    Article Title: Multi-omics profiling and cellular validation reveal distinct lipid networks of krill oil and its predicted mechanisms against insulin resistance
    Article Snippet: LC-MS grade reagents such as methanol and acetonitrile were purchased from Merck; L-2-chlorophenylalanine (≥98%) was purchased from Shanghai Aladdin; formic acid was purchased from TCI; methyl tert-butyl ether (MTBE) was purchased from CNW Technologies. .. Mouse 3T3-L1 preadipocytes (American Type Culture Collection, Manassas, VA, USA) were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Carlsbad, CA, USA) supplemented with 10% newborn calf serum (NBCS; Gibco), 100 U/mL penicillin, and 0.1 μg/mL streptomycin (Gibco) at 37 °C in a humidified atmosphere containing 5% CO 2 . ..

    Article Title: Mesothelial cells maintain energy homeostasis in adipose tissue through the production of interleukin-18.
    Article Snippet: Background: Mesothelial cells, which have long been regarded as an inert cell population, form a depot-specific mesothelium structure surrounding visceral adipose tissue.. Although emerging evidence has elucidated the previously unrecognized functionality of mesothelial cells in adipose tissue, their role in metabolic regulation remains largely unknown.. Methods: Single-cell transcriptomes of mesothelial cells obtained from human omental adipose tissues were analyzed to determine cellular secretory function.

    Article Title: Adipocyte–Tumor Crosstalk and CSC-Associated Doxorubicin Response in Breast Cancer Mammospheres: Role of Licorice Root Extract
    Article Snippet: .. Murine 3T3-L1 preadipocytes (ATCC CL-173, American Type Culture Collection, Manassas, VA, USA) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with fetal bovine serum and antibiotics under standard conditions. ..

    Modification:

    Article Title: Multi-omics profiling and cellular validation reveal distinct lipid networks of krill oil and its predicted mechanisms against insulin resistance
    Article Snippet: LC-MS grade reagents such as methanol and acetonitrile were purchased from Merck; L-2-chlorophenylalanine (≥98%) was purchased from Shanghai Aladdin; formic acid was purchased from TCI; methyl tert-butyl ether (MTBE) was purchased from CNW Technologies. .. Mouse 3T3-L1 preadipocytes (American Type Culture Collection, Manassas, VA, USA) were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Carlsbad, CA, USA) supplemented with 10% newborn calf serum (NBCS; Gibco), 100 U/mL penicillin, and 0.1 μg/mL streptomycin (Gibco) at 37 °C in a humidified atmosphere containing 5% CO 2 . ..

    Article Title: Mesothelial cells maintain energy homeostasis in adipose tissue through the production of interleukin-18.
    Article Snippet: Background: Mesothelial cells, which have long been regarded as an inert cell population, form a depot-specific mesothelium structure surrounding visceral adipose tissue.. Although emerging evidence has elucidated the previously unrecognized functionality of mesothelial cells in adipose tissue, their role in metabolic regulation remains largely unknown.. Methods: Single-cell transcriptomes of mesothelial cells obtained from human omental adipose tissues were analyzed to determine cellular secretory function.

    Article Title: Inhibition of adipocyte-macrophage interaction via CSF1-CSF1R by curcumin.
    Article Snippet: 1 Department of Food Science and Biotechnology, Ewha Womans University, 52 Ewhayeodae-gil, Seodaemun-gu, Seoul 03760, Republic of Korea Abstract Adipocytes (Ad), macrophages (Mac), and lipopolysaccharide (LPS) in the adipose tissue (AT) of individuals with obesity may jointly contribute to obesity-induced pathogenesis.. This study aims to examine the roles of adipocyte− macrophage interaction (AMI) and LPS in obesity-induced pathogenesis, as well as the effects of curcumin (Cur) on these interactions.. An indirect co-culture model using conditioned media (CM) from 3T3-L1 Ad and J774A.1 Mac, with LPS, was used.

    Article Title: Adipocyte–Tumor Crosstalk and CSC-Associated Doxorubicin Response in Breast Cancer Mammospheres: Role of Licorice Root Extract
    Article Snippet: .. Murine 3T3-L1 preadipocytes (ATCC CL-173, American Type Culture Collection, Manassas, VA, USA) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with fetal bovine serum and antibiotics under standard conditions. ..

    Incubator:

    Article Title: Mesothelial cells maintain energy homeostasis in adipose tissue through the production of interleukin-18.
    Article Snippet: Background: Mesothelial cells, which have long been regarded as an inert cell population, form a depot-specific mesothelium structure surrounding visceral adipose tissue.. Although emerging evidence has elucidated the previously unrecognized functionality of mesothelial cells in adipose tissue, their role in metabolic regulation remains largely unknown.. Methods: Single-cell transcriptomes of mesothelial cells obtained from human omental adipose tissues were analyzed to determine cellular secretory function.

    other:

    Article Title: A stromal β-defensin, DEFB124, contributes to cutaneous host defense against Staphylococcus aureus .
    Article Snippet: nih.gov/geo/I) Experimental models: Cell lines 3T3-L1 ATCC Cat# CL-173; RRID:CVCL_0123 Human fibroblast like preadipocytes Cell Applications Cat#802 s-05a Experimental models: Organisms/strains C57BL/6J (C57 Black 6) mice The Jackson Laboratory RRID:IMSR_JAX:000664 Oligonucleotides Defb25 siRNA −1 Invitrogen Part number AM16708, ID #506022 Lot #ASO2MUIW Defb25 siRNA −2 Invitrogen Part number AM16708, ID #506023 Lot #ASO2MZAE Negative control siRNA Invitrogen #AM4613 Il4ra siRNA Invitrogen Cat# s68281 Software and algorithms



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