Journal: International Journal of Dentistry

Article Title: Acinar Cell Proliferation Promoted by BMP2 in Injured Mouse Parotid Gland: BMP2 Promotes Cell Proliferation in Parotid Gland

doi: 10.1155/2023/1765317

Figure Lengend Snippet: Mouse parotid acinar cells in primary culture. (a) Cell proliferative capacity of BMP2-added (100 ng/mL) and nonadded (control) groups after 48 hr of culture. The proliferative capacity of BMP2-added cultured cells was significantly increased compared to that in the control group. ∗∗ Indicates significance at P < 0.01, ∗ P < 0.05. Data were shown as mean ± SD. Three independent experiments were performed. (b) Protein expression of E-cadherin (an epithelial marker) and vimentin (a mesenchymal marker) in cultured cells of each group at 48 hr after the addition of BMP2. NIH3T3 (3T3) was used as a positive control for mesenchymal markers. RS: rat serum.

Article Snippet: NIH3T3 whole cell lysate (Novus Biologicals, Centennial, CO, USA) was used as a positive control for mesenchymal markers.

Techniques: Control, Cell Culture, Expressing, Marker, Positive Control

Journal: Translational Oncology

Article Title: Prognostic Significance and Functional Role of CEP57 in Prostate Cancer 1

doi: 10.1016/j.tranon.2015.11.004

Figure Lengend Snippet: CEP57 overexpression induces microtubule bundling and promotes centriole overduplication. (A) Co-immunofluorescence microscopic analysis of CEP57 and α-tubulin to visualize CEP57-induced microtubule bundles (“basket”) in mouse NIH3T3 cells overexpressing either empty vector (control) or murine (mCEP57). Nuclei stained with DAPI. Scale bar = 10 μm. (B) Immunofluorescence microscopic analysis of centrioles in LNCaP cells expressing centrin-GFP as a centriole marker and transfected with either empty vector (control) or mCEP57. A DsRed encoding plasmid was used as transfection control. Nuclei stained with DAPI. Scale bar = 10 μm. (C) Quantification of overduplicated centrioles (> 4 centrioles per cell, > 1 daughter at a single maternal centriole) in LNCaP cells transfected with centrin-GFP together with an empty vector (control) or mCEP57. Statistical analysis was performed using Student's two-tailed t test for independent samples.

Article Snippet: Human prostate cancer cell lines LNCaP and PC-3 and mouse NIH3T3 cells were obtained from CLS Cell Line Service (Eppelheim, Germany) and maintained according to the distributor's recommendations.

Techniques: Over Expression, Immunofluorescence, Plasmid Preparation, Staining, Expressing, Marker, Transfection, Two Tailed Test

Journal:

Article Title: Biochemical and Proteomic Analysis of the Magnetosome Membrane in Magnetospirillum gryphiswaldense

doi: 10.1128/AEM.70.2.1040-1050.2004

Figure Lengend Snippet: Fatty acid compositions of whole cells and magnetosomes of M. gryphiswaldense MSR-1 as determined by gas chromatography-MS

Article Snippet: M. gryphiswaldense MSR-1 (= DSM 6361), M. magnetotacticum MS-1 (= ATCC 31632), and Magnetospirillum sp. strain AMB-1 (= ATCC 700264) were used in this study.

Techniques: Chromatography