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multiscribe reverse transcriptase using random hexamers  (Thermo Fisher)


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    Structured Review

    Thermo Fisher multiscribe reverse transcriptase using random hexamers
    Multiscribe Reverse Transcriptase Using Random Hexamers, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multiscribe+reverse+transcriptase/multiscribe+reverse+transcriptase+using+random+hexamers/pm40289073-144-5-7
    Average 90 stars, based on 1 article reviews
    multiscribe reverse transcriptase using random hexamers - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Roles of GDF9 and BMP15 in equine follicular development: in vivo content and in vitro effects of IGF1 and cortisol on granulosa cells.
    Article Snippet: Then, 1.3 μg of total RNA from each sample was treated with DNase using the RQ1 RNase-free DNase kit (Promega, Duebendorf, Switzerland). .. The MultiScribe Reverse Transcriptase using random hexamers (Applied Biosystems by Thermo Fisher, Foster City, CA, USA) was applied to synthesize cDNA. .. Using 5 μl of the obtained cDNA corresponding to 100 ng RNA per sample and FastStart Universal Probe Master (ROX, Roche Diagnostics), semiquantitative real time (TaqMan) PCR was performed in duplicate in 96-well optical plates.

    Article Title: Investigation of the Effect of Diabetes on Radiculopathy Induced by Nucleus Pulposus Application to the DRG in a Spontaneously Diabetic Rat Model
    Article Snippet: Absorbance ratios of 260/280 nm and concentrations of total RNA were measured using a NanoDrop 2000 (ThermoFisher Scientific), andRNA integrity numbers29 were assessed on an Agilent 2100 Bioanalyzer (Agilent Technologies) to confirm the quality of total RNA (Table 2). .. For each sample, 1 μg RNA was reverse-transcribed to complementary DNA (cDNA) using random hexamers and MultiScribe reverse transcriptase (ThermoFisher Scientific). qPCR was performed on a Mx3000P system (ThermoFisher Scientific). .. With ahydrolysis probe and primer mixture (TaqMan Gene Expression Assays; ThermoFisher Scientific), the following gene expression was detected: GAP43: Rn01474579_m1, glyceraldehyde-3-phosphate (GAPDH): Rn01775763_g1.

    Real-time Polymerase Chain Reaction:

    Article Title: Investigation of the Effect of Diabetes on Radiculopathy Induced by Nucleus Pulposus Application to the DRG in a Spontaneously Diabetic Rat Model
    Article Snippet: Absorbance ratios of 260/280 nm and concentrations of total RNA were measured using a NanoDrop 2000 (ThermoFisher Scientific), andRNA integrity numbers29 were assessed on an Agilent 2100 Bioanalyzer (Agilent Technologies) to confirm the quality of total RNA (Table 2). .. For each sample, 1 μg RNA was reverse-transcribed to complementary DNA (cDNA) using random hexamers and MultiScribe reverse transcriptase (ThermoFisher Scientific). qPCR was performed on a Mx3000P system (ThermoFisher Scientific). .. With ahydrolysis probe and primer mixture (TaqMan Gene Expression Assays; ThermoFisher Scientific), the following gene expression was detected: GAP43: Rn01474579_m1, glyceraldehyde-3-phosphate (GAPDH): Rn01775763_g1.



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