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multiscribe reverse transcriptase kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher multiscribe reverse transcriptase kit
    Multiscribe Reverse Transcriptase Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multiscribe+reverse+transcriptase/multiscribe+reverse+transcriptase+kit/pmc12260516-107-13-17
    Average 90 stars, based on 1 article reviews
    multiscribe reverse transcriptase kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Isolation:

    Article Title: Micelle-Formulated Juglone Effectively Targets Pancreatic Cancer and Remodels the Tumor Microenvironment.
    Article Snippet: .. RNA was isolated using an RNeasy kit with on-column DNase treatment (Qiagen, Germantown, MD, USA). cDNA was generated using the Multiscribe Reverse Transcriptase kit (Thermo Fisher, Waltham, MA, USA). qPCR analysis was performed with Fast SYBR Green reagent (Invitrogen, Waltham, MA, USA) using a StepOne machine (Applied Biosystems, Waltham, MA, USA). ..

    Generated:

    Article Title: Micelle-Formulated Juglone Effectively Targets Pancreatic Cancer and Remodels the Tumor Microenvironment.
    Article Snippet: .. RNA was isolated using an RNeasy kit with on-column DNase treatment (Qiagen, Germantown, MD, USA). cDNA was generated using the Multiscribe Reverse Transcriptase kit (Thermo Fisher, Waltham, MA, USA). qPCR analysis was performed with Fast SYBR Green reagent (Invitrogen, Waltham, MA, USA) using a StepOne machine (Applied Biosystems, Waltham, MA, USA). ..

    Reverse Transcription:

    Article Title: Micelle-Formulated Juglone Effectively Targets Pancreatic Cancer and Remodels the Tumor Microenvironment.
    Article Snippet: .. RNA was isolated using an RNeasy kit with on-column DNase treatment (Qiagen, Germantown, MD, USA). cDNA was generated using the Multiscribe Reverse Transcriptase kit (Thermo Fisher, Waltham, MA, USA). qPCR analysis was performed with Fast SYBR Green reagent (Invitrogen, Waltham, MA, USA) using a StepOne machine (Applied Biosystems, Waltham, MA, USA). ..

    Article Title: Cancer associated fibroblasts serve as an ovarian cancer stem cell niche through noncanonical Wnt5a signaling.
    Article Snippet: .. Reverse Transcription was done using MultiScribe Reverse Transcriptase kit (Thermo Fisher Scientific, Cat. No. 4311235) according to the manufacturer’s protocol using a Veriti 96-Well Thermal Cycler (Thermo Fisher Scientific). .. Quantitative real-time PCR for ALDH1A1, Wnt5a, SOX2, OCT4, NANOG, and PORCN was performed using TaqMan gene expression assay (Applied Biosystems, Cat. Nos.

    Article Title: Dietary intake and glutamine-serine metabolism control pathologic vascular stiffness.
    Article Snippet: In brief Rachedi et al. identify the precise metabolic requirements for pathogenic vascular fibroblast activation, using pulmonary arterial hypertension as an example.. Inflammation, hypoxia, and mechanical stress converge on YAP and TAZ activation.. YAP and TAZ control glutamine and serine catabolism to generate proline and glycine for collagen biosynthesis.

    Article Title: Adipsin-dependent adipocyte maturation induces cancer cell invasion in breast cancer.
    Article Snippet: .. First-strand cDNA extracted from samples was synthesized from total RNA (1 μg) using a MultiScribe Reverse Transcriptase Kit (Thermo Fisher Scientific). ..

    Article Title: Adult rats sired by obese fathers present learning deficits associated with epigenetic and neurochemical alterations linked to impaired brain glutamatergic signaling.
    Article Snippet: .. Thereafter, 1 μg of purified RNA was treated with DNase and reverse transcribed using the MultiScribeTM Reverse Transcriptase kit from Thermo Fisher or polyadenylated and reverse- transcribed using the Applied BiosystemsTM TaqManTM Advanced miRNA cDNA Synthesis Kit. ..

    Article Title: Targeting Intracellular Innate RNA-Sensing Systems Overcomes Resistance to CAR T-cell Therapy in Solid Tumors
    Article Snippet: Total RNA was extracted from sorted tumor cell pellets using the innuPREP RNA mini kit 2.0 (Analytik Jena). .. RNA concentrations were measured using a NanoDrop spectrophotometer. cDNA was reverse-transcribed using the MultiScribe Reverse Transcriptase kit (Thermo Fisher Scientific). .. Using PowerUp SYBR Green Master Mix (Thermo Fisher Scientific), 2 ng of cDNA was analyzed by qPCR in a QuantStudio 5 Real Time PCR System.

    Article Title: Association of antipsychotic drugs on type 2 diabetes mellitus risk in patients with schizophrenia: a population-based cohort and in vitro glucose homeostasis-related gene expression study.
    Article Snippet: Total RNA was extracted from differentiated HepaRGTM and RD cells using the Direct-zolTM RNA MiniPrep kit (ZYMO Research, Irvine, CA, USA), according to the manufacturer’s instructions. .. RNA quality and quantity were determined by calculating the absorbance at 260/280 nm. cDNA was synthesized from 1 μg of total RNA using a MultiScribeTM reverse transcriptase kit (Thermo Fisher Scientific). .. We performed qRT-PCR using the StepOnePlusTM Real-Time PCR System and Luminaris Color HiGreen qPCR Master Mix (Thermo Fisher Scientific) to measure the expression of hepatic G6Pase, PEPCK, muscular IR-A, IR-B, GLUT4, and ANGPTL4.

    Article Title: Diet-Induced Obesity in the Rat Impairs Sphingolipid Metabolism in the Brain and This Metabolic Dysfunction Is Transmitted to the Offspring via Both the Maternal and the Paternal Lineage.
    Article Snippet: Obesity leads to a number of health problems, including learning and memory deficits that can be passed on to the offspring via a developmental programming process.. However, the mechanisms involved in the deleterious effects of obesity on cognition remain largely unknown.. This study aimed to assess the impact of obesity on the production of sphingolipids (ceramides and sphingomyelins) in the brain and its relationship with the learning deficits displayed by obese individuals.

    Real-time Polymerase Chain Reaction:

    Article Title: Micelle-Formulated Juglone Effectively Targets Pancreatic Cancer and Remodels the Tumor Microenvironment.
    Article Snippet: .. RNA was isolated using an RNeasy kit with on-column DNase treatment (Qiagen, Germantown, MD, USA). cDNA was generated using the Multiscribe Reverse Transcriptase kit (Thermo Fisher, Waltham, MA, USA). qPCR analysis was performed with Fast SYBR Green reagent (Invitrogen, Waltham, MA, USA) using a StepOne machine (Applied Biosystems, Waltham, MA, USA). ..

    SYBR Green Assay:

    Article Title: Micelle-Formulated Juglone Effectively Targets Pancreatic Cancer and Remodels the Tumor Microenvironment.
    Article Snippet: .. RNA was isolated using an RNeasy kit with on-column DNase treatment (Qiagen, Germantown, MD, USA). cDNA was generated using the Multiscribe Reverse Transcriptase kit (Thermo Fisher, Waltham, MA, USA). qPCR analysis was performed with Fast SYBR Green reagent (Invitrogen, Waltham, MA, USA) using a StepOne machine (Applied Biosystems, Waltham, MA, USA). ..

    Lysis:

    Article Title: Dietary intake and glutamine-serine metabolism control pathologic vascular stiffness.
    Article Snippet: In brief Rachedi et al. identify the precise metabolic requirements for pathogenic vascular fibroblast activation, using pulmonary arterial hypertension as an example.. Inflammation, hypoxia, and mechanical stress converge on YAP and TAZ activation.. YAP and TAZ control glutamine and serine catabolism to generate proline and glycine for collagen biosynthesis.

    Collagen Assay:

    Article Title: Dietary intake and glutamine-serine metabolism control pathologic vascular stiffness.
    Article Snippet: In brief Rachedi et al. identify the precise metabolic requirements for pathogenic vascular fibroblast activation, using pulmonary arterial hypertension as an example.. Inflammation, hypoxia, and mechanical stress converge on YAP and TAZ activation.. YAP and TAZ control glutamine and serine catabolism to generate proline and glycine for collagen biosynthesis.

    Negative Control:

    Article Title: Dietary intake and glutamine-serine metabolism control pathologic vascular stiffness.
    Article Snippet: In brief Rachedi et al. identify the precise metabolic requirements for pathogenic vascular fibroblast activation, using pulmonary arterial hypertension as an example.. Inflammation, hypoxia, and mechanical stress converge on YAP and TAZ activation.. YAP and TAZ control glutamine and serine catabolism to generate proline and glycine for collagen biosynthesis.

    Recombinant:

    Article Title: Dietary intake and glutamine-serine metabolism control pathologic vascular stiffness.
    Article Snippet: In brief Rachedi et al. identify the precise metabolic requirements for pathogenic vascular fibroblast activation, using pulmonary arterial hypertension as an example.. Inflammation, hypoxia, and mechanical stress converge on YAP and TAZ activation.. YAP and TAZ control glutamine and serine catabolism to generate proline and glycine for collagen biosynthesis.

    Plasmid Preparation:

    Article Title: Dietary intake and glutamine-serine metabolism control pathologic vascular stiffness.
    Article Snippet: In brief Rachedi et al. identify the precise metabolic requirements for pathogenic vascular fibroblast activation, using pulmonary arterial hypertension as an example.. Inflammation, hypoxia, and mechanical stress converge on YAP and TAZ activation.. YAP and TAZ control glutamine and serine catabolism to generate proline and glycine for collagen biosynthesis.

    Software:

    Article Title: Dietary intake and glutamine-serine metabolism control pathologic vascular stiffness.
    Article Snippet: In brief Rachedi et al. identify the precise metabolic requirements for pathogenic vascular fibroblast activation, using pulmonary arterial hypertension as an example.. Inflammation, hypoxia, and mechanical stress converge on YAP and TAZ activation.. YAP and TAZ control glutamine and serine catabolism to generate proline and glycine for collagen biosynthesis.

    Synthesized:

    Article Title: Adipsin-dependent adipocyte maturation induces cancer cell invasion in breast cancer.
    Article Snippet: .. First-strand cDNA extracted from samples was synthesized from total RNA (1 μg) using a MultiScribe Reverse Transcriptase Kit (Thermo Fisher Scientific). ..

    Article Title: Association of antipsychotic drugs on type 2 diabetes mellitus risk in patients with schizophrenia: a population-based cohort and in vitro glucose homeostasis-related gene expression study.
    Article Snippet: Total RNA was extracted from differentiated HepaRGTM and RD cells using the Direct-zolTM RNA MiniPrep kit (ZYMO Research, Irvine, CA, USA), according to the manufacturer’s instructions. .. RNA quality and quantity were determined by calculating the absorbance at 260/280 nm. cDNA was synthesized from 1 μg of total RNA using a MultiScribeTM reverse transcriptase kit (Thermo Fisher Scientific). .. We performed qRT-PCR using the StepOnePlusTM Real-Time PCR System and Luminaris Color HiGreen qPCR Master Mix (Thermo Fisher Scientific) to measure the expression of hepatic G6Pase, PEPCK, muscular IR-A, IR-B, GLUT4, and ANGPTL4.

    Purification:

    Article Title: Adult rats sired by obese fathers present learning deficits associated with epigenetic and neurochemical alterations linked to impaired brain glutamatergic signaling.
    Article Snippet: .. Thereafter, 1 μg of purified RNA was treated with DNase and reverse transcribed using the MultiScribeTM Reverse Transcriptase kit from Thermo Fisher or polyadenylated and reverse- transcribed using the Applied BiosystemsTM TaqManTM Advanced miRNA cDNA Synthesis Kit. ..

    Article Title: Diet-Induced Obesity in the Rat Impairs Sphingolipid Metabolism in the Brain and This Metabolic Dysfunction Is Transmitted to the Offspring via Both the Maternal and the Paternal Lineage.
    Article Snippet: Obesity leads to a number of health problems, including learning and memory deficits that can be passed on to the offspring via a developmental programming process.. However, the mechanisms involved in the deleterious effects of obesity on cognition remain largely unknown.. This study aimed to assess the impact of obesity on the production of sphingolipids (ceramides and sphingomyelins) in the brain and its relationship with the learning deficits displayed by obese individuals.

    cDNA Synthesis:

    Article Title: Adult rats sired by obese fathers present learning deficits associated with epigenetic and neurochemical alterations linked to impaired brain glutamatergic signaling.
    Article Snippet: .. Thereafter, 1 μg of purified RNA was treated with DNase and reverse transcribed using the MultiScribeTM Reverse Transcriptase kit from Thermo Fisher or polyadenylated and reverse- transcribed using the Applied BiosystemsTM TaqManTM Advanced miRNA cDNA Synthesis Kit. ..

    Spectrophotometry:

    Article Title: Targeting Intracellular Innate RNA-Sensing Systems Overcomes Resistance to CAR T-cell Therapy in Solid Tumors
    Article Snippet: Total RNA was extracted from sorted tumor cell pellets using the innuPREP RNA mini kit 2.0 (Analytik Jena). .. RNA concentrations were measured using a NanoDrop spectrophotometer. cDNA was reverse-transcribed using the MultiScribe Reverse Transcriptase kit (Thermo Fisher Scientific). .. Using PowerUp SYBR Green Master Mix (Thermo Fisher Scientific), 2 ng of cDNA was analyzed by qPCR in a QuantStudio 5 Real Time PCR System.



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