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M.J Research Inc multiscribe reverse transcriptase (50u/µl)
Multiscribe Reverse Transcriptase (50u/µl), supplied by M.J Research Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multiscribe+reverse+transcriptase/multiscribe+reverse+transcriptase++50u+%CE%BCl+/pmc04960001-139-16-31
Average 90 stars, based on 1 article reviews
multiscribe reverse transcriptase (50u/µl) - by Bioz Stars, 2026-09
90/100 stars

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Reverse Transcription:

Article Title: QRFP in Female Rats: Effects on High Fat Food Intake and Hypothalamic Gene Expression across the Estrous Cycle
Article Snippet: Reverse transcription (RT) was conducted using the High-Capacity cDNA Reverse Transcriptase Kit (Applied Biosystems, Foster City, CA, USA). .. For RT, 1.0μg of RNA from each sample was added to random primers (10×), dNTP (25×), MultiScribe Reverse Transcriptase (50U/μl) and RT buffer (10×) and incubated in a thermal cycler (PTC-100, MJ Research, Inc, Watertown, MA, USA) for 10 min at 27°C, then for 120 min at 37°C. .. Taqman Gene Expression Assays (Applied Biosystems) were used to assess levels of prepro-QRFP (Rn01644297_s1), GPR103a (Rn01644293_mH), GPR103b (Rn01425102_m1) and the housekeeping gene, cyclophilin (Rn00574762_m1), therefore primer sequence information is unavailable.

Article Title: CD36 mRNA in the Gastrointestinal Tract Is Differentially Regulated by Dietary Fat Intake in Obesity-Prone and Obesity-Resistant Rats
Article Snippet: Reverse transcription (RT) was conducted using the High-Capacity cDNA Reverse Transcriptase Kit (Applied Biosystems, Foster City, CA, USA). .. For RT, 2.0 μg RNA from each sample was added to random primers (10×), dNTP (25×), MultiScribe Reverse Transcriptase (50U/μl), and RT buffer (10×) and incubated in a thermal cycler (PTC-100; MJ Research, Inc., Watertown, MA, USA) for 10 min at 27 °C, then for 120 min at 37 °C. .. Primers were designed using Primer Express (Applied Biosystems, Foster City, CA, USA).

Incubation:

Article Title: QRFP in Female Rats: Effects on High Fat Food Intake and Hypothalamic Gene Expression across the Estrous Cycle
Article Snippet: Reverse transcription (RT) was conducted using the High-Capacity cDNA Reverse Transcriptase Kit (Applied Biosystems, Foster City, CA, USA). .. For RT, 1.0μg of RNA from each sample was added to random primers (10×), dNTP (25×), MultiScribe Reverse Transcriptase (50U/μl) and RT buffer (10×) and incubated in a thermal cycler (PTC-100, MJ Research, Inc, Watertown, MA, USA) for 10 min at 27°C, then for 120 min at 37°C. .. Taqman Gene Expression Assays (Applied Biosystems) were used to assess levels of prepro-QRFP (Rn01644297_s1), GPR103a (Rn01644293_mH), GPR103b (Rn01425102_m1) and the housekeeping gene, cyclophilin (Rn00574762_m1), therefore primer sequence information is unavailable.

Article Title: The effects of high fat diet and estradiol on hypothalamic prepro-QRFP mRNA expression in female rats
Article Snippet: Reverse transcription (RT) was conducted using the High-Capacity cDNA Reverse Transcriptase Kit (Applied Biosystems/Life Technologies, Foster City, CA, USA). .. For RT, 1.0µg of RNA from each sample was added to random primers (10×), dNTP (25×), MultiScribe Reverse Transcriptase (50U/µl) and RT buffer (10×) and incubated in a thermal cycler (PTC-100, MJ Research, Inc, Watertown, MA, USA) for 10 min at 27°C, then for 120 min at 37°C. .. Taqman Gene Expression Assays (Applied Biosystems/Life Technologies) were used to assess levels of prepro-QRFP mRNA (Rn01644297_s1), QRFP-r1 (Rn01644293_mH), QRFP-r2 (Rn01425102_m1) and the housekeeping gene, cyclophilin (Rn00452692_m1).

Article Title: CD36 mRNA in the Gastrointestinal Tract Is Differentially Regulated by Dietary Fat Intake in Obesity-Prone and Obesity-Resistant Rats
Article Snippet: Reverse transcription (RT) was conducted using the High-Capacity cDNA Reverse Transcriptase Kit (Applied Biosystems, Foster City, CA, USA). .. For RT, 2.0 μg RNA from each sample was added to random primers (10×), dNTP (25×), MultiScribe Reverse Transcriptase (50U/μl), and RT buffer (10×) and incubated in a thermal cycler (PTC-100; MJ Research, Inc., Watertown, MA, USA) for 10 min at 27 °C, then for 120 min at 37 °C. .. Primers were designed using Primer Express (Applied Biosystems, Foster City, CA, USA).



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