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multiscribe-reverse transcriptase kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher multiscribe-reverse transcriptase kit
    Multiscribe Reverse Transcriptase Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multiscribe+reverse+transcriptase+kit/pm39692827-245-7-10
    Average 90 stars, based on 1 article reviews
    multiscribe-reverse transcriptase kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Functional screening identifies miRNAs with a novel function inhibiting Vascular Smooth Muscle Cell proliferation.
    Article Snippet: 24 Proliferation of vascular smooth muscle cells (vSMCs) is a crucial contributor to pathological 25 vascular remodelling.. MicroRNAs (miRNAs) are powerful gene regulators and attractive 26 therapeutic agents.. Here, we aim to systematically identify and characterise miRNAs with 27 therapeutic potential in targeting vSMC proliferation.

    Article Title: Functional screening identifies novel miRNAs inhibiting Vascular Smooth Muscle Cell proliferation
    Article Snippet: All RNA samples were stored at −80°C until required. miRNA reverse transcription (RT) to cDNA utilized the Applied Biosystems miRNA Reverse Transcription Kit. cDNA was synthesised from total RNA (2ng/μl RNA per reaction) using the MultiScribeTM Reverse Transcriptase kit (Life Technologies, Paisley, UK) and miRNA-specific RT probes.

    Article Title: HSP and CD279 gene expression as candidate biomarkers in symptomatic LGLL patients.
    Article Snippet: Synthesis of cDNA was performed with the MultiScribe-Reverse Transcriptase kit (Applied Biosystems).

    Article Title: Association of antipsychotic drugs on type 2 diabetes mellitus risk in patients with schizophrenia: a population-based cohort and in vitro glucose homeostasis-related gene expression study
    Article Snippet: RNA quality and quantity were determined by calculating the absorbance at 260/280 nm. cDNA was synthesized from 1 μg of total RNA using a MultiScribeTM reverse transcriptase kit (Thermo Fisher Scientific).

    Article Title: The role of ALDH1A1 in glioblastoma proliferation and invasion.
    Article Snippet: SYBR® Green PCR Master Mix and the MultiScribeTM Reverse Transcriptase Kit were sourced from Applied Biosystems (Foster City, CA, USA).

    Synthesized:

    Article Title: Identification of RNA-based cell-type markers for stem-cell manufacturing systems with a statistical scoring function
    Article Snippet: Total RNA was extracted from cultured cells using Trizol reagent (Invitrogen, USA). .. First-strand cDNA was synthesized from total RNA using M-MLV reverse transcriptase kit with the stem-loop RT primer of miRNAs and using Multiscribe Reverse Transcriptase kit (Applied Biosystems) with random primers according to the manufacturer's protocol. .. Real-time qRT-PCR was performed for miRNAs and U6 using the SYBR Green master mix (Bimake).

    Reverse Transcription:

    Article Title: Identification of RNA-based cell-type markers for stem-cell manufacturing systems with a statistical scoring function
    Article Snippet: Total RNA was extracted from cultured cells using Trizol reagent (Invitrogen, USA). .. First-strand cDNA was synthesized from total RNA using M-MLV reverse transcriptase kit with the stem-loop RT primer of miRNAs and using Multiscribe Reverse Transcriptase kit (Applied Biosystems) with random primers according to the manufacturer's protocol. .. Real-time qRT-PCR was performed for miRNAs and U6 using the SYBR Green master mix (Bimake).

    Article Title: Activation of Peroxisome Proliferator-Activated Receptor-β/δ (PPARβ/δ) in Keratinocytes by Endogenous Fatty Acids
    Article Snippet: .. The cDNA was generated using 2.5 μg total RNA with the MultiScribe Reverse Transcriptase kit (Applied Biosystems, Foster City, CA, USA). .. Primers were designed for real-time PCR using the Primer Express software (Version 3.0, Applied Biosystems, Foster City, CA, USA).

    Article Title: Interaction between a J-domain co-chaperone and a specific Argonaute protein contributes to microRNA function in animals
    Article Snippet: .. For mRNA quantification, reverse transcription was performed using 400 ng of RNA with Multiscribe Reverse Transcriptase Kit (Applied Biosystems) (25°C for 10 min, 37°C for 120 min and 85°C for 5 min) followed by RNase H treatment (1 ul per RT) at 37°C for 30 min. SYBR Green RT-qPCR was performed using 2 ul of cDNA with the SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) on a QuantStudio 3 machine with regular settings (95°C for 10 min and 40 cycles at 95°C for 15 s and 60°C for 60 s). ..

    Generated:

    Article Title: Activation of Peroxisome Proliferator-Activated Receptor-β/δ (PPARβ/δ) in Keratinocytes by Endogenous Fatty Acids
    Article Snippet: .. The cDNA was generated using 2.5 μg total RNA with the MultiScribe Reverse Transcriptase kit (Applied Biosystems, Foster City, CA, USA). .. Primers were designed for real-time PCR using the Primer Express software (Version 3.0, Applied Biosystems, Foster City, CA, USA).

    SYBR Green Assay:

    Article Title: Interaction between a J-domain co-chaperone and a specific Argonaute protein contributes to microRNA function in animals
    Article Snippet: .. For mRNA quantification, reverse transcription was performed using 400 ng of RNA with Multiscribe Reverse Transcriptase Kit (Applied Biosystems) (25°C for 10 min, 37°C for 120 min and 85°C for 5 min) followed by RNase H treatment (1 ul per RT) at 37°C for 30 min. SYBR Green RT-qPCR was performed using 2 ul of cDNA with the SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) on a QuantStudio 3 machine with regular settings (95°C for 10 min and 40 cycles at 95°C for 15 s and 60°C for 60 s). ..

    Quantitative RT-PCR:

    Article Title: Interaction between a J-domain co-chaperone and a specific Argonaute protein contributes to microRNA function in animals
    Article Snippet: .. For mRNA quantification, reverse transcription was performed using 400 ng of RNA with Multiscribe Reverse Transcriptase Kit (Applied Biosystems) (25°C for 10 min, 37°C for 120 min and 85°C for 5 min) followed by RNase H treatment (1 ul per RT) at 37°C for 30 min. SYBR Green RT-qPCR was performed using 2 ul of cDNA with the SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) on a QuantStudio 3 machine with regular settings (95°C for 10 min and 40 cycles at 95°C for 15 s and 60°C for 60 s). ..



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