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multiscribe reverse transcriptase (high capacity cdna archive kit)  (Thermo Fisher)


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    Structured Review

    Thermo Fisher multiscribe reverse transcriptase (high capacity cdna archive kit)
    Multiscribe Reverse Transcriptase (High Capacity Cdna Archive Kit), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multiscribe+reverse+transcriptase+kit/multiscribe+reverse+transcriptase/pm39965324-78-15-20
    Average 90 stars, based on 1 article reviews
    multiscribe reverse transcriptase (high capacity cdna archive kit) - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Method and system for photoacoustic imaging of tissue and organ fibrosis
    Article Snippet: Statistical analysis was performed using Matlab 2018b (MathWorks, Inc., Natick, MA). .. RNA was then reverse transcribed into cDNA with Multiscribe Reverse Transcriptase (Thermo Fisher Scientific, Cat. #4311235), and levels of Col1a1, Col3a1, Col4a1, Acta2, and/or Gapdh were quantified. ..

    Article Title: Expression of a protein disulfide isomerase A3 variant associated with amyotrophic lateral sclerosis triggers disease features in mice.
    Article Snippet: Chemiluminescence signal and protein ladder images were acquired using the ChemiDoc Imaging System (BioRad). .. For RT-PCR, cDNA was synthesized from a total of 1 μg of RNA using Multiscribe Reverse Transcriptase (ThermoFisher Scientific) and random primers p(dN)6 (Roche) according to manufacturer’s instructions. .. Conventional RT-PCR was performed using GoTaq Green Master Mix (Promega).

    Article Title: Dipeptidyl peptidase DPF-3 is a gatekeeper of microRNA Argonaute compensation in animals
    Article Snippet: .. We have included a description in the figure legend regarding how our RT-qPCR data is normalized and clarified it in the Methods section as follows (line 528): “The RNA was reverse transcribed using Multiscribe reverse transcriptase (ThermoFisher). .. TaqMan probes specific to the assessed miRNAs were used to measure miRNA levels, while SYBR Green master mix (Biorad) with target-specific oligonucleotides was employed to assess the expression levels of the miRNA targets.

    Article Title: Statins may enhance the chemosensitivity of R-CHOP in diffuse large B-cell lymphoma
    Article Snippet: [ 24 ] Briefly, total RNA was isolated with the Purelink RNA kit (Invitrogen) according to the manufactureŕs instructions. .. One microgram RNA was reverse transcribed by using the Multiscribe Reverse Transcriptase (Thermo Fischer) and the complementary DNA (cDNA) was used for a FAM-based real-time PCR. .. A standard protocol was used for the analysis of gene expressions (Applied Biosystems).

    Article Title: Lifetime Variations of Prolactin Receptor Isoforms mRNA in the Hippocampus and Dentate Gyrus of the Rat-Effects of Aging.
    Article Snippet: Isolation was performed using RNeasy Mini and QIAshredder Kits (Qiagen N.V., Venlo, The Netherlands #74104 and #79654) according to the manufacturer’s instructions. .. RNA was stored at −80 ◦C and used for cDNA synthesis using random primers and MultiScribe Reverse Transcriptase (Thermo Fisher Scientific). ..

    Article Title: Lifetime Variations of Prolactin Receptor Isoforms mRNA in the Hippocampus and Dentate Gyrus of the Rat—Effects of Aging
    Article Snippet: Isolation was performed using RNeasy Mini and QIAshredder Kits (Qiagen N.V., Venlo, The Netherlands #74104 and #79654) according to the manufacturer’s instructions. .. RNA was stored at −80 °C and used for cDNA synthesis using random primers and MultiScribe Reverse Transcriptase (Thermo Fisher Scientific). ..

    Article Title: Dipeptidyl peptidase DPF-3 is a gatekeeper of microRNA Argonaute compensation in animals.
    Article Snippet: .. The RNA was reverse transcribed with Multiscribe reverse transcriptase (Thermo Fisher Scientific). .. TaqMan probes specific for the assessedmiRNAswere used for themeasurement ofmiRNA levels, and SYBR green master mix (Biorad) with target-specific oligonucleotides were used to measure miRNA targets expression levels.

    Article Title: Regulation of MORC-1 is key to the CSR-1–mediated germline gene licensing mechanism in C. elegans
    Article Snippet: .. cDNA for quantitation of mRNA levels was made from 500 ng of total RNA using Multiscribe Reverse Transcriptase (Applied Biosystems) and random hexamer primers in an Eppendorf Mastercycler Pro S6325 (Eppendorf). qPCR for mRNA levels was performed with Absolute Blue SYBR Green (Thermo Fisher Scientific) and normalized to eft-2 or him-3 [in experiments using the MORC-1 overexpression strain ( morcOE )] using a CFX63 Real Time System Thermocycler (Bio-Rad). ..

    Synthesized:

    Article Title: Expression of a protein disulfide isomerase A3 variant associated with amyotrophic lateral sclerosis triggers disease features in mice.
    Article Snippet: Chemiluminescence signal and protein ladder images were acquired using the ChemiDoc Imaging System (BioRad). .. For RT-PCR, cDNA was synthesized from a total of 1 μg of RNA using Multiscribe Reverse Transcriptase (ThermoFisher Scientific) and random primers p(dN)6 (Roche) according to manufacturer’s instructions. .. Conventional RT-PCR was performed using GoTaq Green Master Mix (Promega).

    Quantitative RT-PCR:

    Article Title: Dipeptidyl peptidase DPF-3 is a gatekeeper of microRNA Argonaute compensation in animals
    Article Snippet: .. We have included a description in the figure legend regarding how our RT-qPCR data is normalized and clarified it in the Methods section as follows (line 528): “The RNA was reverse transcribed using Multiscribe reverse transcriptase (ThermoFisher). .. TaqMan probes specific to the assessed miRNAs were used to measure miRNA levels, while SYBR Green master mix (Biorad) with target-specific oligonucleotides was employed to assess the expression levels of the miRNA targets.

    Real-time Polymerase Chain Reaction:

    Article Title: Statins may enhance the chemosensitivity of R-CHOP in diffuse large B-cell lymphoma
    Article Snippet: [ 24 ] Briefly, total RNA was isolated with the Purelink RNA kit (Invitrogen) according to the manufactureŕs instructions. .. One microgram RNA was reverse transcribed by using the Multiscribe Reverse Transcriptase (Thermo Fischer) and the complementary DNA (cDNA) was used for a FAM-based real-time PCR. .. A standard protocol was used for the analysis of gene expressions (Applied Biosystems).

    Article Title: Regulation of MORC-1 is key to the CSR-1–mediated germline gene licensing mechanism in C. elegans
    Article Snippet: .. cDNA for quantitation of mRNA levels was made from 500 ng of total RNA using Multiscribe Reverse Transcriptase (Applied Biosystems) and random hexamer primers in an Eppendorf Mastercycler Pro S6325 (Eppendorf). qPCR for mRNA levels was performed with Absolute Blue SYBR Green (Thermo Fisher Scientific) and normalized to eft-2 or him-3 [in experiments using the MORC-1 overexpression strain ( morcOE )] using a CFX63 Real Time System Thermocycler (Bio-Rad). ..

    cDNA Synthesis:

    Article Title: Lifetime Variations of Prolactin Receptor Isoforms mRNA in the Hippocampus and Dentate Gyrus of the Rat-Effects of Aging.
    Article Snippet: Isolation was performed using RNeasy Mini and QIAshredder Kits (Qiagen N.V., Venlo, The Netherlands #74104 and #79654) according to the manufacturer’s instructions. .. RNA was stored at −80 ◦C and used for cDNA synthesis using random primers and MultiScribe Reverse Transcriptase (Thermo Fisher Scientific). ..

    Article Title: Lifetime Variations of Prolactin Receptor Isoforms mRNA in the Hippocampus and Dentate Gyrus of the Rat—Effects of Aging
    Article Snippet: Isolation was performed using RNeasy Mini and QIAshredder Kits (Qiagen N.V., Venlo, The Netherlands #74104 and #79654) according to the manufacturer’s instructions. .. RNA was stored at −80 °C and used for cDNA synthesis using random primers and MultiScribe Reverse Transcriptase (Thermo Fisher Scientific). ..

    Quantitation Assay:

    Article Title: Regulation of MORC-1 is key to the CSR-1–mediated germline gene licensing mechanism in C. elegans
    Article Snippet: .. cDNA for quantitation of mRNA levels was made from 500 ng of total RNA using Multiscribe Reverse Transcriptase (Applied Biosystems) and random hexamer primers in an Eppendorf Mastercycler Pro S6325 (Eppendorf). qPCR for mRNA levels was performed with Absolute Blue SYBR Green (Thermo Fisher Scientific) and normalized to eft-2 or him-3 [in experiments using the MORC-1 overexpression strain ( morcOE )] using a CFX63 Real Time System Thermocycler (Bio-Rad). ..

    Random Hexamer:

    Article Title: Regulation of MORC-1 is key to the CSR-1–mediated germline gene licensing mechanism in C. elegans
    Article Snippet: .. cDNA for quantitation of mRNA levels was made from 500 ng of total RNA using Multiscribe Reverse Transcriptase (Applied Biosystems) and random hexamer primers in an Eppendorf Mastercycler Pro S6325 (Eppendorf). qPCR for mRNA levels was performed with Absolute Blue SYBR Green (Thermo Fisher Scientific) and normalized to eft-2 or him-3 [in experiments using the MORC-1 overexpression strain ( morcOE )] using a CFX63 Real Time System Thermocycler (Bio-Rad). ..

    SYBR Green Assay:

    Article Title: Regulation of MORC-1 is key to the CSR-1–mediated germline gene licensing mechanism in C. elegans
    Article Snippet: .. cDNA for quantitation of mRNA levels was made from 500 ng of total RNA using Multiscribe Reverse Transcriptase (Applied Biosystems) and random hexamer primers in an Eppendorf Mastercycler Pro S6325 (Eppendorf). qPCR for mRNA levels was performed with Absolute Blue SYBR Green (Thermo Fisher Scientific) and normalized to eft-2 or him-3 [in experiments using the MORC-1 overexpression strain ( morcOE )] using a CFX63 Real Time System Thermocycler (Bio-Rad). ..

    Over Expression:

    Article Title: Regulation of MORC-1 is key to the CSR-1–mediated germline gene licensing mechanism in C. elegans
    Article Snippet: .. cDNA for quantitation of mRNA levels was made from 500 ng of total RNA using Multiscribe Reverse Transcriptase (Applied Biosystems) and random hexamer primers in an Eppendorf Mastercycler Pro S6325 (Eppendorf). qPCR for mRNA levels was performed with Absolute Blue SYBR Green (Thermo Fisher Scientific) and normalized to eft-2 or him-3 [in experiments using the MORC-1 overexpression strain ( morcOE )] using a CFX63 Real Time System Thermocycler (Bio-Rad). ..



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