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WholeGenome LLC mpseq
Mpseq, supplied by WholeGenome LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mpseq/mpseq/pmc10119592-183-0-11
Average 90 stars, based on 1 article reviews
mpseq - by Bioz Stars, 2026-09
90/100 stars

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Article Title: Chromosomal Junction Detection from Whole-Genome Sequencing on Formalin-Fixed, Paraffin-Embedded Tumors.
Article Snippet: Chromosomal Junction Detection from Whole-Genome Sequencing on Formalin-Fixed, Paraffin-Embedded Tumors Stephen Murphy,* James Smadbeck,* Bruce Eckloff,y Yean Lee,y Sarah Johnson,* Giannoula Karagouga,* Vishnu Serla,*z Anurag Sharma,* Robert Sikkink,y Jesse Voss,z Faye Harris,* Janet Schaefer Kline,* Farhad Kosari,* Andrew Feldman,z Eric Wieben,yx Marie Christine Aubry,z Benjamin Kipp,z Jin Jen,*yz John Cheville,*z and George Vasmatzis*

Sequencing:

Article Title: Optical genome mapping in acute myeloid leukemia: a multicenter evaluation
Article Snippet: .. MPseq is a powerful technique for detecting genomic fusions in a wholegenome approach and obviates some of the ambiguity associated with short-read sequencing. ..



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( A ) Blood is drawn before and after <t>surgery.</t> <t>DNA</t> from tumor is sequenced using the next-generation mate-pair sequencing <t>(MPseq)</t> protocol to identify chromosomal rearrangements. Several junctions are chosen to construct a personalized panel for each tumor. Percent of ctDNA out of total cfDNA is calculated at each time point of blood collection. ( B ) Number of junctions identified in cohort of 10 cases of serous stage 3 ovarian cancer. Count numbers are above the bars with the expected false negative count in parenthesis. cfDNA: cell-free DNA; ctDNA: circulating tumor DNA.
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( A ) Blood is drawn before and after surgery. DNA from tumor is sequenced using the next-generation mate-pair sequencing (MPseq) protocol to identify chromosomal rearrangements. Several junctions are chosen to construct a personalized panel for each tumor. Percent of ctDNA out of total cfDNA is calculated at each time point of blood collection. ( B ) Number of junctions identified in cohort of 10 cases of serous stage 3 ovarian cancer. Count numbers are above the bars with the expected false negative count in parenthesis. cfDNA: cell-free DNA; ctDNA: circulating tumor DNA.

Journal: Scientific Reports

Article Title: Quantification of Somatic Chromosomal Rearrangements in Circulating Cell-Free DNA from Ovarian Cancers

doi: 10.1038/srep29831

Figure Lengend Snippet: ( A ) Blood is drawn before and after surgery. DNA from tumor is sequenced using the next-generation mate-pair sequencing (MPseq) protocol to identify chromosomal rearrangements. Several junctions are chosen to construct a personalized panel for each tumor. Percent of ctDNA out of total cfDNA is calculated at each time point of blood collection. ( B ) Number of junctions identified in cohort of 10 cases of serous stage 3 ovarian cancer. Count numbers are above the bars with the expected false negative count in parenthesis. cfDNA: cell-free DNA; ctDNA: circulating tumor DNA.

Article Snippet: DNA from primary tumor was prepared using the Illumina MPseq Kit following the manufacturer’s instructions and sequenced as two libraries per lane on the Illumina HiSeq2000 platform .

Techniques: Sequencing, Construct