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mate pair sequencing (mpseq)  (Illumina Inc)


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    Illumina Inc mate pair sequencing (mpseq)
    Mate Pair Sequencing (Mpseq), supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mpseq/mate+pair+sequencing/pmc05091324-83-19-9
    Average 90 stars, based on 1 article reviews
    mate pair sequencing (mpseq) - by Bioz Stars, 2026-11
    90/100 stars

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    other:

    Article Title: De Novo Genome Sequencing, Annotation, and Taxonomy of Unknown Bacteria
    Article Snippet: A complete genome can usually be obtained for bacteria by using “mate-pair” sequencing ( Weber and Myers 1997 ; see Protocol: Preparation of an 8-kb Mate-Pair Library for Illumina Sequencing [ Mardis and McCombie 2017 ]), in which long DNA molecules are circularized and the sequences flanking the ligation junctions are sequenced.

    Article Title: Advances in Genome Sequencing and Natural Rubber Biosynthesis in Rubber-Producing Plants
    Article Snippet: , RRIM600 , 1.55 Gb , 84,440 , 67.24 Kb , Illumina and mate-pair sequencing , 2016 , [ ] .

    Article Title: A Chromosome-Level Assembly of Blunt Snout Bream ( Megalobrama amblycephala ) Genome Reveals an Expansion of Olfactory Receptor Genes in Freshwater Fish
    Article Snippet: We produced a high quality reference genome using a combination of PacBio long reads ( , online) and Illumina mate pair and paired end sequencing.

    Article Title: SICaRiO: short indel call filtering with boosting.
    Article Snippet: Illumina [mate pair (https:// www.illumina.com/science/technology/next-generation-seque ncing/mate-pair-sequencing.html)] with BWA-MEM [53] and GATK comes next with a base precision of around 62.33%.

    Article Title: A novel Bacteroides metallo-β-lactamase (MBL) and its gene ( crxA ) in Bacteroides xylanisolvens revealed by genomic sequencing and functional analysis
    Article Snippet: WGS of B. xylanisolvens 14880 was performed using Illumina mate-paired sequencing technology, the resulting sequence was submitted to GenBank ( www.ncbi.nlm.nih.gov , acc. no. PRJNA481227) and the WGS data were further analysed using ResFinder and IslandViewer 4 for the presence of antibiotic resistance genes (80% coverage and 30% homology parameters) and genomic islands, respectively.,

    Sequencing:

    Article Title: ForageGrassBase: molecular resource for the forage grass meadow fescue ( Festuca pratensis Huds.)
    Article Snippet: .. De novo sequencing of the meadow fescue genomes were performed using Illumina mate pair sequencing and assembly was performed by the SOAPdenovo2 assembler ( ). .. De novo sequencing of the meadow fescue genomes were performed using Illumina mate pair sequencing and assembly was performed by the SOAPdenovo2 assembler ( ).

    Article Title: Next generation sequencing
    Article Snippet: .. This is not possible using existing technologies such as the ILLUMINA® mate pair sequencing, which allows distant sequences to be identified, yet does not reliably and unequivocally identify the entire junction sequence due to the presence of gaps, especially in the repeat regions, and the reliance on a reference mandated by short read NGS. .. Random N primer extension kits are commonly available from NEB (Ipswich, MA), Thermo Fisher Scientific (Carlsbad, CA), Stratagene (La Jolla, CA), and Agilent (Santa Clara, CA), to name but a few, and the extension conducted according to the manufacturer's directions.

    Next-Generation Sequencing:

    Article Title: Next generation sequencing
    Article Snippet: .. This is not possible using existing technologies such as the ILLUMINA® mate pair sequencing, which allows distant sequences to be identified, yet does not reliably and unequivocally identify the entire junction sequence due to the presence of gaps, especially in the repeat regions, and the reliance on a reference mandated by short read NGS. .. Random N primer extension kits are commonly available from NEB (Ipswich, MA), Thermo Fisher Scientific (Carlsbad, CA), Stratagene (La Jolla, CA), and Agilent (Santa Clara, CA), to name but a few, and the extension conducted according to the manufacturer's directions.



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    illumina mpseq kit  (Illumina Inc)
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    Illumina Inc illumina mpseq kit
    ( A ) Blood is drawn before and after <t>surgery.</t> <t>DNA</t> from tumor is sequenced using the next-generation mate-pair sequencing <t>(MPseq)</t> protocol to identify chromosomal rearrangements. Several junctions are chosen to construct a personalized panel for each tumor. Percent of ctDNA out of total cfDNA is calculated at each time point of blood collection. ( B ) Number of junctions identified in cohort of 10 cases of serous stage 3 ovarian cancer. Count numbers are above the bars with the expected false negative count in parenthesis. cfDNA: cell-free DNA; ctDNA: circulating tumor DNA.
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    ( A ) Blood is drawn before and after surgery. DNA from tumor is sequenced using the next-generation mate-pair sequencing (MPseq) protocol to identify chromosomal rearrangements. Several junctions are chosen to construct a personalized panel for each tumor. Percent of ctDNA out of total cfDNA is calculated at each time point of blood collection. ( B ) Number of junctions identified in cohort of 10 cases of serous stage 3 ovarian cancer. Count numbers are above the bars with the expected false negative count in parenthesis. cfDNA: cell-free DNA; ctDNA: circulating tumor DNA.

    Journal: Scientific Reports

    Article Title: Quantification of Somatic Chromosomal Rearrangements in Circulating Cell-Free DNA from Ovarian Cancers

    doi: 10.1038/srep29831

    Figure Lengend Snippet: ( A ) Blood is drawn before and after surgery. DNA from tumor is sequenced using the next-generation mate-pair sequencing (MPseq) protocol to identify chromosomal rearrangements. Several junctions are chosen to construct a personalized panel for each tumor. Percent of ctDNA out of total cfDNA is calculated at each time point of blood collection. ( B ) Number of junctions identified in cohort of 10 cases of serous stage 3 ovarian cancer. Count numbers are above the bars with the expected false negative count in parenthesis. cfDNA: cell-free DNA; ctDNA: circulating tumor DNA.

    Article Snippet: DNA from primary tumor was prepared using the Illumina MPseq Kit following the manufacturer’s instructions and sequenced as two libraries per lane on the Illumina HiSeq2000 platform .

    Techniques: Sequencing, Construct