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nebnext companion module  (New England Biolabs)


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    Structured Review

    New England Biolabs nebnext companion module
    Nebnext Companion Module, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/module/NEBNext+Companion+Module+for+Oxford/pm41872458-301-12-12
    Average 97 stars, based on 34 article reviews
    nebnext companion module - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Ligation:

    Article Title: Genome sequence of Escherichia coli H1G2: a B1 lineage isolated from the gut of Hilsa shad ( Tenualosa ilisha ) of Bangladesh.
    Article Snippet: Genomic DNA from an overnight (37°C) EMB culture was extracted using the FavorPrep Tissue Genomic DNA Extraction HE Mini Kit (Favorgen, Taiwan) and quantified with a Qubit 4.0 fluorometer (Thermo Fisher Scientific). .. Libraries were prepared using the Oxford Nanopore Technologies (ONT) Ligation Sequencing Kit SQK-NBD114.24 with the NEBNext companion module (New England Biolabs) and Native Barcoding Kit 96 (v14) (no shearing and no size selection) and sequenced on an ONT MinION using an R10.4.1 flow cell (6). ..

    Article Title: Enhancing low-level genome-edited crop detection and identification in food mixtures using nanopore adaptive sampling: Rice-Soybean mixture as proof-of-concept.
    Article Snippet: Using the NEB Bio Calculator 39, the observed fragment size was used to calculate the sample dilution needed for optimal molarity (50-60 fmol per load) compatible with ONT AS sequencing requirements 18. .. The library preparation was performed with Ligation sequencing kit V14 (SQK-LSK114) (Oxford Nanopore Technologies (ONT), Oxford, United Kingdom) and the NebNext Companion module v2 (New England Biolabs, Ipswich, Massachusetts, United States of America), according to the manufacturer’s instructions. ..

    Article Title: Psychrobacillus syltrankelensis sp. nov., a new species isolated from soil in the North Caucasus, Russia.
    Article Snippet: Genomic DNA for Oxford Nanopore sequencing was isolated using the MagBeads FastDNA Kit for Microbiome (MP Biomedicals, Santa Ana, CA, USA) according to the manufacturer’s instructions. .. Libraries were prepared following the manufacturer’s protocol with the Native Barcoding Kit 24 V14 (Oxford Nanopore Technologies Ltd, Oxford, UK), the NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing (New England Biolabs, Ipswich, MA, USA), NEBNext Quick Ligation Reaction Buffer, and Blunt/TA Ligase Master Mix (New England Biolabs). .. Sequencing was performed on a MinION Mk1B platform (Oxford Nanopore Technologies Ltd) using an R10 flow cell.

    Article Title: Nested mobile genetic elements mediating antimicrobial resistance genes mobility within and between Acinetobacter isolates
    Article Snippet: Blunt/TA Ligase Master Mix , New England BioLabs , M03367L. .. NEBNext® Companion Module for Oxford Nanopore Technologies Ligation Sequencing , New England BioLabs , E7180S. .. NEBNext® Quick Ligation Reaction Buffer , New England BioLabs , B6058S.

    Article Title: Long-Read Haplotype Phasing Resolves Allelic Configuration as a Missing Layer of Precision Oncology
    Article Snippet: Samples with a high proportion of short DNA fragments were further processed using the BluePippin High-Pass DNA Size Selection System (Sage Science) to enrich for fragments greater than 5 kb, suitable for nanopore sequencing. .. Sequencing libraries were prepared using the Ligation Sequencing Kit V14 (SQK-LSK114; Oxford Nanopore Technologies) in combination with the NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing (E7180S; New England Biolabs), following the manufacturers’ protocols. ..

    Article Title: Phenotypic divergence is driven by mobile genetic elements in a heritable insect symbiont
    Article Snippet: .. We extracted DNA using a Qiagen DNeasy kit and conducted library prep using Oxford Nanopore Technologies v.14 ligation kit and the NEB companion module v.2 and sequenced on an R10.4.1 flow cell for 24 h to generate sequence data. ..

    Article Title: Genome sequence of the ornamental plant Digitalis purpurea reveals the molecular basis of flower color and morphology variation
    Article Snippet: .. The initial DNA repair was performed with two enzyme mixes of the NEBNext ® Companion Module Oxford Nanopore Technologies ® (ONT) Ligation Sequencing (E7180L) following the suppliers’ instructions. ..

    Sequencing:

    Article Title: Genome sequence of Escherichia coli H1G2: a B1 lineage isolated from the gut of Hilsa shad ( Tenualosa ilisha ) of Bangladesh.
    Article Snippet: Genomic DNA from an overnight (37°C) EMB culture was extracted using the FavorPrep Tissue Genomic DNA Extraction HE Mini Kit (Favorgen, Taiwan) and quantified with a Qubit 4.0 fluorometer (Thermo Fisher Scientific). .. Libraries were prepared using the Oxford Nanopore Technologies (ONT) Ligation Sequencing Kit SQK-NBD114.24 with the NEBNext companion module (New England Biolabs) and Native Barcoding Kit 96 (v14) (no shearing and no size selection) and sequenced on an ONT MinION using an R10.4.1 flow cell (6). ..

    Article Title: Enhancing low-level genome-edited crop detection and identification in food mixtures using nanopore adaptive sampling: Rice-Soybean mixture as proof-of-concept.
    Article Snippet: Using the NEB Bio Calculator 39, the observed fragment size was used to calculate the sample dilution needed for optimal molarity (50-60 fmol per load) compatible with ONT AS sequencing requirements 18. .. The library preparation was performed with Ligation sequencing kit V14 (SQK-LSK114) (Oxford Nanopore Technologies (ONT), Oxford, United Kingdom) and the NebNext Companion module v2 (New England Biolabs, Ipswich, Massachusetts, United States of America), according to the manufacturer’s instructions. ..

    Article Title: Psychrobacillus syltrankelensis sp. nov., a new species isolated from soil in the North Caucasus, Russia.
    Article Snippet: Genomic DNA for Oxford Nanopore sequencing was isolated using the MagBeads FastDNA Kit for Microbiome (MP Biomedicals, Santa Ana, CA, USA) according to the manufacturer’s instructions. .. Libraries were prepared following the manufacturer’s protocol with the Native Barcoding Kit 24 V14 (Oxford Nanopore Technologies Ltd, Oxford, UK), the NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing (New England Biolabs, Ipswich, MA, USA), NEBNext Quick Ligation Reaction Buffer, and Blunt/TA Ligase Master Mix (New England Biolabs). .. Sequencing was performed on a MinION Mk1B platform (Oxford Nanopore Technologies Ltd) using an R10 flow cell.

    Article Title: Benchmarking of shotgun sequencing depth reveals the potential and limitations of shallow metagenomics and strain-level analysis
    Article Snippet: .. The Mock-stag-70 DNA (200 fmol) was prepared for long-read sequencing with the SQK-LSK114 kit (Oxford Nanopore Technologies), following the manufacturer’s protocol and the NEBNext Companion module v2 (New England Biomedicals). .. Approximately 80 fmol of a >20-kb library was loaded onto an R10.4.1 flowcell (FLO-PRO114, Oxford Nanopore Technologies) and sequenced for 72 h on a Promethion P2 solo (Oxford Nanopore Technologies) using MinKNOW v.25.05.12.

    Article Title: Nested mobile genetic elements mediating antimicrobial resistance genes mobility within and between Acinetobacter isolates
    Article Snippet: Blunt/TA Ligase Master Mix , New England BioLabs , M03367L. .. NEBNext® Companion Module for Oxford Nanopore Technologies Ligation Sequencing , New England BioLabs , E7180S. .. NEBNext® Quick Ligation Reaction Buffer , New England BioLabs , B6058S.

    Article Title: Long-Read Haplotype Phasing Resolves Allelic Configuration as a Missing Layer of Precision Oncology
    Article Snippet: Samples with a high proportion of short DNA fragments were further processed using the BluePippin High-Pass DNA Size Selection System (Sage Science) to enrich for fragments greater than 5 kb, suitable for nanopore sequencing. .. Sequencing libraries were prepared using the Ligation Sequencing Kit V14 (SQK-LSK114; Oxford Nanopore Technologies) in combination with the NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing (E7180S; New England Biolabs), following the manufacturers’ protocols. ..

    Article Title: Phenotypic divergence is driven by mobile genetic elements in a heritable insect symbiont
    Article Snippet: .. We extracted DNA using a Qiagen DNeasy kit and conducted library prep using Oxford Nanopore Technologies v.14 ligation kit and the NEB companion module v.2 and sequenced on an R10.4.1 flow cell for 24 h to generate sequence data. ..

    Article Title: Genome sequence of the ornamental plant Digitalis purpurea reveals the molecular basis of flower color and morphology variation
    Article Snippet: .. The initial DNA repair was performed with two enzyme mixes of the NEBNext ® Companion Module Oxford Nanopore Technologies ® (ONT) Ligation Sequencing (E7180L) following the suppliers’ instructions. ..

    Size Selection:

    Article Title: Genome sequence of Escherichia coli H1G2: a B1 lineage isolated from the gut of Hilsa shad ( Tenualosa ilisha ) of Bangladesh.
    Article Snippet: Genomic DNA from an overnight (37°C) EMB culture was extracted using the FavorPrep Tissue Genomic DNA Extraction HE Mini Kit (Favorgen, Taiwan) and quantified with a Qubit 4.0 fluorometer (Thermo Fisher Scientific). .. Libraries were prepared using the Oxford Nanopore Technologies (ONT) Ligation Sequencing Kit SQK-NBD114.24 with the NEBNext companion module (New England Biolabs) and Native Barcoding Kit 96 (v14) (no shearing and no size selection) and sequenced on an ONT MinION using an R10.4.1 flow cell (6). ..



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    Image Search Results


    Journal: iScience

    Article Title: Gapmers targeting conserved SARS-CoV-2 single-stranded regions reduce viral load and reshape inflammatory macrophage responses

    doi: 10.1016/j.isci.2026.117639

    Figure Lengend Snippet:

    Article Snippet: End Prep library kit , NEB , E7546.

    Techniques: Virus, Variant Assay, Recombinant, Reverse Transcription, Diagnostic Assay, Luminex, Ligation, Multiplex Assay, Generated, Software

    Journal: iScience

    Article Title: Gapmers targeting conserved SARS-CoV-2 single-stranded regions reduce viral load and reshape inflammatory macrophage responses

    doi: 10.1016/j.isci.2026.117639

    Figure Lengend Snippet:

    Article Snippet: Libraries were prepared by blunting and dA-tailing the amplicons using the End Prep library kit (NEB; Ref: E7546) followed by NEBNext Ultra II Ligation Module kit (NEB, Ref: E7595) with the adapters obtained from NEBNext Multiplex Oligos for Illumina (NEB, Ref:E7600) following standard protocols.

    Techniques: Virus, Variant Assay, Recombinant, Reverse Transcription, Diagnostic Assay, Luminex, Ligation, Multiplex Assay, Generated, Software

    Journal: iScience

    Article Title: A method for expressing two proteins at a tunable ratio allows for studying the kinetics of creatine transport by MCT12

    doi: 10.1016/j.isci.2026.117719

    Figure Lengend Snippet:

    Article Snippet: Clampfit software , Molecular Devices , RRID: SCR_011323.

    Techniques: Cell-Signaling, Virus, Recombinant, Clinical Proteomics, Membrane, Staining, Cloning, Software