nebnext companion module (New England Biolabs)
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Nebnext Companion Module, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/module/NEBNext+Companion+Module+for+Oxford/pm41872458-301-12-12
Average 97 stars, based on 34 article reviews
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Ligation:Article Title: Genome sequence of Escherichia coli H1G2: a B1 lineage isolated from the gut of Hilsa shad ( Tenualosa ilisha ) of Bangladesh. Article Snippet: Genomic DNA from an overnight (37°C) EMB culture was extracted using the FavorPrep Tissue Genomic DNA Extraction HE Mini Kit (Favorgen, Taiwan) and quantified with a Qubit 4.0 fluorometer (Thermo Fisher Scientific). .. Libraries were prepared using the Oxford Nanopore Technologies (ONT) Ligation Sequencing Kit SQK-NBD114.24 with the Article Title: Enhancing low-level genome-edited crop detection and identification in food mixtures using nanopore adaptive sampling: Rice-Soybean mixture as proof-of-concept. Article Snippet: Using the NEB Bio Calculator 39, the observed fragment size was used to calculate the sample dilution needed for optimal molarity (50-60 fmol per load) compatible with ONT AS sequencing requirements 18. .. The library preparation was performed with Ligation sequencing kit V14 (SQK-LSK114) (Oxford Nanopore Technologies (ONT), Oxford, United Kingdom) and the Article Title: Psychrobacillus syltrankelensis sp. nov., a new species isolated from soil in the North Caucasus, Russia. Article Snippet: Genomic DNA for Oxford Nanopore sequencing was isolated using the MagBeads FastDNA Kit for Microbiome (MP Biomedicals, Santa Ana, CA, USA) according to the manufacturer’s instructions. .. Libraries were prepared following the manufacturer’s protocol with the Native Barcoding Kit 24 V14 (Oxford Nanopore Technologies Ltd, Oxford, UK), the Article Title: Nested mobile genetic elements mediating antimicrobial resistance genes mobility within and between Acinetobacter isolates Article Snippet: Blunt/TA Ligase Master Mix , New England BioLabs , M03367L. .. Article Title: Long-Read Haplotype Phasing Resolves Allelic Configuration as a Missing Layer of Precision Oncology Article Snippet: Samples with a high proportion of short DNA fragments were further processed using the BluePippin High-Pass DNA Size Selection System (Sage Science) to enrich for fragments greater than 5 kb, suitable for nanopore sequencing. .. Sequencing libraries were prepared using the Ligation Sequencing Kit V14 (SQK-LSK114; Oxford Nanopore Technologies) in combination with the Article Title: Phenotypic divergence is driven by mobile genetic elements in a heritable insect symbiont Article Snippet: .. We extracted DNA using a Qiagen DNeasy kit and conducted library prep using Oxford Nanopore Technologies v.14 ligation kit and the Article Title: Genome sequence of the ornamental plant Digitalis purpurea reveals the molecular basis of flower color and morphology variation Article Snippet: .. The initial DNA repair was performed with two enzyme mixes of the Sequencing:Article Title: Genome sequence of Escherichia coli H1G2: a B1 lineage isolated from the gut of Hilsa shad ( Tenualosa ilisha ) of Bangladesh. Article Snippet: Genomic DNA from an overnight (37°C) EMB culture was extracted using the FavorPrep Tissue Genomic DNA Extraction HE Mini Kit (Favorgen, Taiwan) and quantified with a Qubit 4.0 fluorometer (Thermo Fisher Scientific). .. Libraries were prepared using the Oxford Nanopore Technologies (ONT) Ligation Sequencing Kit SQK-NBD114.24 with the Article Title: Enhancing low-level genome-edited crop detection and identification in food mixtures using nanopore adaptive sampling: Rice-Soybean mixture as proof-of-concept. Article Snippet: Using the NEB Bio Calculator 39, the observed fragment size was used to calculate the sample dilution needed for optimal molarity (50-60 fmol per load) compatible with ONT AS sequencing requirements 18. .. The library preparation was performed with Ligation sequencing kit V14 (SQK-LSK114) (Oxford Nanopore Technologies (ONT), Oxford, United Kingdom) and the Article Title: Psychrobacillus syltrankelensis sp. nov., a new species isolated from soil in the North Caucasus, Russia. Article Snippet: Genomic DNA for Oxford Nanopore sequencing was isolated using the MagBeads FastDNA Kit for Microbiome (MP Biomedicals, Santa Ana, CA, USA) according to the manufacturer’s instructions. .. Libraries were prepared following the manufacturer’s protocol with the Native Barcoding Kit 24 V14 (Oxford Nanopore Technologies Ltd, Oxford, UK), the Article Title: Benchmarking of shotgun sequencing depth reveals the potential and limitations of shallow metagenomics and strain-level analysis Article Snippet: .. The Mock-stag-70 DNA (200 fmol) was prepared for long-read sequencing with the SQK-LSK114 kit (Oxford Nanopore Technologies), following the manufacturer’s protocol and the Article Title: Nested mobile genetic elements mediating antimicrobial resistance genes mobility within and between Acinetobacter isolates Article Snippet: Blunt/TA Ligase Master Mix , New England BioLabs , M03367L. .. Article Title: Long-Read Haplotype Phasing Resolves Allelic Configuration as a Missing Layer of Precision Oncology Article Snippet: Samples with a high proportion of short DNA fragments were further processed using the BluePippin High-Pass DNA Size Selection System (Sage Science) to enrich for fragments greater than 5 kb, suitable for nanopore sequencing. .. Sequencing libraries were prepared using the Ligation Sequencing Kit V14 (SQK-LSK114; Oxford Nanopore Technologies) in combination with the Article Title: Phenotypic divergence is driven by mobile genetic elements in a heritable insect symbiont Article Snippet: .. We extracted DNA using a Qiagen DNeasy kit and conducted library prep using Oxford Nanopore Technologies v.14 ligation kit and the Article Title: Genome sequence of the ornamental plant Digitalis purpurea reveals the molecular basis of flower color and morphology variation Article Snippet: .. The initial DNA repair was performed with two enzyme mixes of the Size Selection:Article Title: Genome sequence of Escherichia coli H1G2: a B1 lineage isolated from the gut of Hilsa shad ( Tenualosa ilisha ) of Bangladesh. Article Snippet: Genomic DNA from an overnight (37°C) EMB culture was extracted using the FavorPrep Tissue Genomic DNA Extraction HE Mini Kit (Favorgen, Taiwan) and quantified with a Qubit 4.0 fluorometer (Thermo Fisher Scientific). .. Libraries were prepared using the Oxford Nanopore Technologies (ONT) Ligation Sequencing Kit SQK-NBD114.24 with the |


