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mirnas microarray analysis microrna 4.0 array  (Thermo Fisher)


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    Thermo Fisher mirnas microarray analysis microrna 4.0 array
    Validation of candidate <t>miRNAs.</t> Among the top rated nine miRNAs screened from our miRNA <t>microarray,</t> four miRNAs involving (A) hsa-miR-145-5p, (B) hsa-miR-497-5p, (C) hsa-miR-29a-3p and (D) hsa-miR-204-5p were also significantly altered in GSE40355. miRNA, microRNA.
    Mirnas Microarray Analysis Microrna 4.0 Array, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microrna+microarray+analysis/pmc05779952-163-7-10
    Average 90 stars, based on 1 article reviews
    mirnas microarray analysis microrna 4.0 array - by Bioz Stars, 2026-09
    90/100 stars

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    1) Product Images from "Identification and bioinformatics analysis of miRNAs associated with human muscle invasive bladder cancer"

    Article Title: Identification and bioinformatics analysis of miRNAs associated with human muscle invasive bladder cancer

    Journal: Molecular Medicine Reports

    doi: 10.3892/mmr.2017.7726

    Validation of candidate miRNAs. Among the top rated nine miRNAs screened from our miRNA microarray, four miRNAs involving (A) hsa-miR-145-5p, (B) hsa-miR-497-5p, (C) hsa-miR-29a-3p and (D) hsa-miR-204-5p were also significantly altered in GSE40355. miRNA, microRNA.
    Figure Legend Snippet: Validation of candidate miRNAs. Among the top rated nine miRNAs screened from our miRNA microarray, four miRNAs involving (A) hsa-miR-145-5p, (B) hsa-miR-497-5p, (C) hsa-miR-29a-3p and (D) hsa-miR-204-5p were also significantly altered in GSE40355. miRNA, microRNA.

    Techniques Used: Microarray

    Related Articles

    Microarray:

    Article Title: Identification and bioinformatics analysis of miRNAs associated with human muscle invasive bladder cancer
    Article Snippet: In order to remove genomic DNA, DNase I digestion (cat. no. 79253, Qiagen GmbH), DNase I digestion (cat. no. 79254, Qiagen GmbH) was used in each RNA preparation. .. After assessing RNA quality and quantity, the miRNAs microarray analysis (Affymetrix microRNA 4.0 Array, Affymetrix, Inc., Santa Clara, CA, USA) was performed according to the manufacturer's instructions. .. Briefly, 1 μg of total RNA was labeled with Biotin using the FlashTag Biotin HSR RNA Labeling kit (Genisphere LLC, Hatfield, PA, USA) and then hybridized overnight with the array, which was washed, stained, and read by an GeneChip Scanner 3000 7G (Affymetrix, Inc.).

    Article Title: MicroRNA-455-3p as a potential peripheral biomarker for Alzheimer’s disease
    Article Snippet: .. Primary miRNAs screening by Affymetrix microarray Detailed miRNAs screening of the serum samples were conducted in the University of Texas Southwestern Medical Center, Genomics and Microarray Core Facility, Dallas. .. The miRNA expression profiles were generated with Affymetrix GeneChip miRNA array v. 4.0 (Affymetrix).

    Article Title: miRNome landscape analysis reveals a 30 miRNA core in retinoblastoma
    Article Snippet: .. Total RNA was tailed and biotinylated using Affymetrix Flash-tag biotin for miRNAs microarray (Affymetrtix, USA) and spike-in control probes were added according to manufacturer instructions. ..

    Article Title: Smad proteins bind a conserved RNA sequence to promote microRNA maturation by Drosha
    Article Snippet: .. To identify miRNAs regulated by R-Smads similarly to miR-21 and miR-199a ( Davis et al., 2008 ), we performed a miRNA microarray profiling analysis (Applied Biosystems) of PASMCs stimulated with BMP4 or TGFβ for 24 hr. ..

    Article Title: Inhibiting MicroRNA-503 and MicroRNA-181d with Losartan Ameliorates Diabetic Nephropathy in KKAy Mice
    Article Snippet: .. The purity (A 260/280 ≥1.80) and the quantity of extracted glomerulus RNA meet the requirements of Affymetrix miRNAs microarray experiments. .. miRNA expression profiling was assessed by Affymetrix GeneChip ® miRNAs arrays according to the manufacturer’s instructions (Affymetrix).

    Article Title: The use of miRNA microarrays for the analysis of cancer samples with global miRNA decrease
    Article Snippet: .. To determine whether miRNA microarray profiling could accurately identify samples with global miRNA decrease, we decided to analyze miRNA levels following Dicer1 deletion ( , ), using a single-color miRNA microarray platform (Affymetrix). .. The robust multichip average (RMA) algorithm is a standard method for background correcting, summarizing, and normalizing data from Affymetrix gene expression GeneChips ( ).

    other:

    Article Title: Identification of microRNA-mRNA networks involved in cisplatin-induced renal tubular epithelial cells injury.
    Article Snippet: Cisplatin is a widely used chemotherapeutic drug that often causes acute kidney injury (AKI) in cancer patients.. The contribution of miRNAs to the cisplatin-induced renal tubular epithelial cell injury remains largely unknown.. Here we performed an

    Article Title: Differences in expression of genes related to drug resistance and miRNAs regulating their expression in skin fibroblasts exposed to adalimumab and cyclosporine A
    Article Snippet: Next, in order to select miRNAs that differentiate the anti-TNF drug-exposed culture from the control culture, a miRNA microarray expression profile (GeneChip® miRNA 2.0 Arrays; Affymetrix, Santa Clara, CA) was assessed.

    Control:

    Article Title: miRNome landscape analysis reveals a 30 miRNA core in retinoblastoma
    Article Snippet: .. Total RNA was tailed and biotinylated using Affymetrix Flash-tag biotin for miRNAs microarray (Affymetrtix, USA) and spike-in control probes were added according to manufacturer instructions. ..



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    Validation of candidate <t>miRNAs.</t> Among the top rated nine miRNAs screened from our miRNA <t>microarray,</t> four miRNAs involving (A) hsa-miR-145-5p, (B) hsa-miR-497-5p, (C) hsa-miR-29a-3p and (D) hsa-miR-204-5p were also significantly altered in GSE40355. miRNA, microRNA.
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    Image Search Results


    Flowchart of microarray data analysis. Swine monocytes were exposed to Bb12 with or without the addition of a blocking antibody anti-TLR2. Groups were as follows: unstimulated swine monocytes, monocytes stimulated with Bb12 for 4 h, and swine monocytes incubated with anti-TLR2 antibody and Bb12 for 4 h. Microarray was performed, and 40 microRNAs with a MFI > 500 were selected for analysis of molecular interactions (KEGG analysis). The microRNAs were also analyzed for miRNA–mRNA interactions within TLR2 pathway targets (miRTarBase). After this, 15 miRNAs with a reported interaction with TLR2 pathway–related target proteins were selected to analyze with a multiple t test

    Journal: Probiotics and Antimicrobial Proteins

    Article Title: Immunomodulation by Bifidobacterium animalis subsp. lactis Bb12: Integrative Analysis of miRNA Expression and TLR2 Pathway–Related Target Proteins in Swine Monocytes

    doi: 10.1007/s12602-021-09816-1

    Figure Lengend Snippet: Flowchart of microarray data analysis. Swine monocytes were exposed to Bb12 with or without the addition of a blocking antibody anti-TLR2. Groups were as follows: unstimulated swine monocytes, monocytes stimulated with Bb12 for 4 h, and swine monocytes incubated with anti-TLR2 antibody and Bb12 for 4 h. Microarray was performed, and 40 microRNAs with a MFI > 500 were selected for analysis of molecular interactions (KEGG analysis). The microRNAs were also analyzed for miRNA–mRNA interactions within TLR2 pathway targets (miRTarBase). After this, 15 miRNAs with a reported interaction with TLR2 pathway–related target proteins were selected to analyze with a multiple t test

    Article Snippet: A total of 2 μg RNA from each sample was sent for genome-wide microRNA microarray analysis using μParaflo® microfluidic biochip technology; this service was provided by LC Sciences (Houston, TX, USA).

    Techniques: Microarray, Blocking Assay, Incubation

    Comparison of qRT-PCR and microarray results. Log2 fold change expression between unstimulated monocytes and Bb12-stimulated cells by qRT-PCR and microarray

    Journal: Probiotics and Antimicrobial Proteins

    Article Title: Immunomodulation by Bifidobacterium animalis subsp. lactis Bb12: Integrative Analysis of miRNA Expression and TLR2 Pathway–Related Target Proteins in Swine Monocytes

    doi: 10.1007/s12602-021-09816-1

    Figure Lengend Snippet: Comparison of qRT-PCR and microarray results. Log2 fold change expression between unstimulated monocytes and Bb12-stimulated cells by qRT-PCR and microarray

    Article Snippet: A total of 2 μg RNA from each sample was sent for genome-wide microRNA microarray analysis using μParaflo® microfluidic biochip technology; this service was provided by LC Sciences (Houston, TX, USA).

    Techniques: Comparison, Quantitative RT-PCR, Microarray, Expressing

    Mir-92b promoted osteogenesis in MSCs (A–B) The top three up/down-regulated microRNAs in De-Os-MSCs were listed, and verified by qPCR (B) (C–E) The scrambled control, let-7e, mir-10b, mir-20a, mir-92b, mir-371 and mir-373 were transduced into MSCs with lentiviruses. The overexpression of each microRNA was verified by qPCR (C). The infected MSCs were induced to undergo osteogenic differentiation for 10 days, then the calcium deposits were stained with Alizarin Red S (D), and quantified (E) (F) Total RNA was extracted from MSCs infected with mir-92b or scrambled control. The mRNA expression levels of Osterix, Runx2, OPN and ALP were detected by qPCR. β-actin was used as an internal control. The data was expressed as mean ​± ​SD (n ​= ​3). ∗p ​< ​0.05 (G–I) Total proteins were extracted from MSCs transduced with scrambled control or mir-92b. Then the proteins were analyzed by western blot using indicated antibodies. The protein levels of pERK (H) and pJNK (I) was normalized to ERK and JNK1 respectively. All the data represent mean ​± ​SD of three independent experiments. ∗p ​< ​0.05 (J–K) The mir-92b antagmir was transfected into MSCs, then the cells were treated with osteogenic induction medium for 10 days, the calcium deposits were stained with Alizarin Red S (J), the changes of osteogenesis-related genes was checked by qPCR (K).

    Journal: Journal of Orthopaedic Translation

    Article Title: De-osteogenic-differentiated mesenchymal stem cells accelerate fracture healing by mir-92b

    doi: 10.1016/j.jot.2020.10.009

    Figure Lengend Snippet: Mir-92b promoted osteogenesis in MSCs (A–B) The top three up/down-regulated microRNAs in De-Os-MSCs were listed, and verified by qPCR (B) (C–E) The scrambled control, let-7e, mir-10b, mir-20a, mir-92b, mir-371 and mir-373 were transduced into MSCs with lentiviruses. The overexpression of each microRNA was verified by qPCR (C). The infected MSCs were induced to undergo osteogenic differentiation for 10 days, then the calcium deposits were stained with Alizarin Red S (D), and quantified (E) (F) Total RNA was extracted from MSCs infected with mir-92b or scrambled control. The mRNA expression levels of Osterix, Runx2, OPN and ALP were detected by qPCR. β-actin was used as an internal control. The data was expressed as mean ​± ​SD (n ​= ​3). ∗p ​< ​0.05 (G–I) Total proteins were extracted from MSCs transduced with scrambled control or mir-92b. Then the proteins were analyzed by western blot using indicated antibodies. The protein levels of pERK (H) and pJNK (I) was normalized to ERK and JNK1 respectively. All the data represent mean ​± ​SD of three independent experiments. ∗p ​< ​0.05 (J–K) The mir-92b antagmir was transfected into MSCs, then the cells were treated with osteogenic induction medium for 10 days, the calcium deposits were stained with Alizarin Red S (J), the changes of osteogenesis-related genes was checked by qPCR (K).

    Article Snippet: The microRNA microarray analysis was performed by the Annoroad Gene Technology Corporation (Beijing, China).

    Techniques: Control, Over Expression, Infection, Staining, Expressing, Transduction, Western Blot, Transfection

    Validation of candidate miRNAs. Among the top rated nine miRNAs screened from our miRNA microarray, four miRNAs involving (A) hsa-miR-145-5p, (B) hsa-miR-497-5p, (C) hsa-miR-29a-3p and (D) hsa-miR-204-5p were also significantly altered in GSE40355. miRNA, microRNA.

    Journal: Molecular Medicine Reports

    Article Title: Identification and bioinformatics analysis of miRNAs associated with human muscle invasive bladder cancer

    doi: 10.3892/mmr.2017.7726

    Figure Lengend Snippet: Validation of candidate miRNAs. Among the top rated nine miRNAs screened from our miRNA microarray, four miRNAs involving (A) hsa-miR-145-5p, (B) hsa-miR-497-5p, (C) hsa-miR-29a-3p and (D) hsa-miR-204-5p were also significantly altered in GSE40355. miRNA, microRNA.

    Article Snippet: After assessing RNA quality and quantity, the miRNAs microarray analysis (Affymetrix microRNA 4.0 Array, Affymetrix, Inc., Santa Clara, CA, USA) was performed according to the manufacturer's instructions.

    Techniques: Microarray